مقایسه سمیت و جذب کادمیوم از دو ترکیب پلیمری و نیتراتی توسط ذرت تلقیح شده با قارچ Glomus caledonium

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشدبیولوژی و بیوتکنولوژی خاک- گروه خاکشناسی- دانشکده کشاورزی- دانشگاه شهید چمران اهواز- اهواز- ایران

2 استادیار بیولوژی و بیوتکنولوژی خاک- گروه خاکشناسی- دانشکده کشاورزی- دانشگاه شهید چمران اهواز- اهواز- ایران

3 استادیار گروه شیمی- دانشکده علوم- دانشگاه شهید چمران اهواز- اهواز- ایران

چکیده

در دهه‌های اخیر رشد صنعت سبب افزایش آلودگی خاک‌های کشاورزی به فلزات سنگین شده است و استفاده از قارچ میکوریزا می‌تواند بر نحوه جذب این فلزات به گیاه تأثیرگذار باشد. لذا این تحقیق با استفاده از گیاه ذرت در شرایط گلخانه‌ای با دو فاکتور کادمیوم (شامل سطوح صفر، mg Cd kg-150 با پلیمر Poly-hydroxybutanamide و mg Cd kg-150 با نیترات کادمیوم) و فاکتور قارچ (با قارچ میکوریزا Glomus Caledonium و بدون قارچ) به‌صورت فاکتوریل و در قالب طرح پایۀ کاملاً تصادفی در سه تکرار انجام شد. آلودگی کادمیوم منجر به کاهش معنی‌دار (0.05≥P) عملکرد اندام هوایی (از g pot-105/31 به 34/26 و 10/27)، غلظت فسفر شاخساره (از g kg-1 37/0 به 36/0 و 36/0)، کربوهیدرات خاک (از  mg g-167/12 به 40/10 و 81/9) و نیز منجر به افزایش معنی‌دار گلومالین خاک (از  ug g-1 56/458 به 37/600 و 635) به ترتیب از تیمار شاهد به پلیمر-Cd و نیترات-Cd مشاهده گردید. استفاده از قارچ میکوریزا سبب کاهش جذب کادمیوم به گیاه، افزایش گلومالین خاک و بهبود پارامترهای موردبررسی شد. نتایج این مطالعه نقش کلیدی و مهم گلومالین را در پاسخ به شرایط تنش آلودگی کادمیومی نشان می‌دهد. کاربرد ترکیب پلیمر-کادمیوم (به‌عنوان یک ترکیب غیر‌ سمی)، دسترسی فلز سنگین برای گیاه را افزایش داد و منجر به جذب بیشتر گیاه (mg kg-191/34) نسبت به تیمار نیترات کادمیوم (mg kg-183/19) شد که می‌تواند در ارتقاء توان گیاه‌پالایی و به‌عنوان راهکاری مؤثر در افزایش کارآیی گیاه‌پالایی خاک‌های آلوده به فلزات سنگین مورد استفاده قرار گیرد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Comparison of Cadmium Toxicity and Absorption from Polymer-Cd and Nitrate-Cd by Corn Inoculated with Glomus caledonium

نویسندگان [English]

  • Milad Babadi 1
  • Roya Zalaghi 2
  • Mehdi Taghavi 3
1 Master student of soil biology and biotechnology- soil science department- Shahid Chamran university of Ahvaz- Ahvaz- Iran
2 Assistant professor of soil biology and biotechnology-soil science department- Shahid Chamran University of Ahvaz- Ahvaz-Iran
3 Assistant professor of chemistry department- science faculty- Shahid Chamran University of Ahvaz- Ahvaz-Iran
چکیده [English]

In the recent decades, the industrial revolution resulted in contamination of the biosphere by heavy metals and mycorrhizal fungi could affect the metals uptake by the plant. This research was carried out using Zea mays with three levels of soil Cd cotamination (0, 50 mg kg-1 using Polymer (Poly-hydroxybutanamide) – Cd, and 50 mg kg-1 using Cd-nitrate) and two levels of mycorrhizal (inoculanted with Glomus Caledonium and non-inoculanted) with three replications in a factorial experiment as a completely randomized design in greenhouse conditions. Cadmium pollution caused a significant reduction (P≥0.05) in shoot dry weight (from 31.05 to 26.34 and 27.10 g pot-1), shoot phosphorus concentration (from 0.37 to 0.36 and 0.36 g kg-1), soil carbohydrate (from 12.67 to 10.40 and 9.81 mg g-1) and also resulted an increases in soil glomalin (from 458.56 to 600.37 and 635 µg g-1) from control to polymer-Cd and nitrate-Cd respectively. Inoculation with mycorrhizal fungi reduced Cd uptake by the Zea mays, increased the soil glomalin content and improved the soil biological parameters. The results of this study showed that glomalin is an important protein in response to stress condition of Cd contamination. Poly-hydroxybutanamide polymer (a non-toxic compound) increased Cd availability and Cd uptake by plant (34.91 mg kg-1) compared to nitrate-Cd (19.83 mg kg-1) and it could be recommended to improve phytoremediation.

کلیدواژه‌ها [English]

  • Cadmium
  • Corn
  • Arbuscular-vesicular mycorrhiza
  • Glomalin
  • Carbohydrate
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