حذف آنتی‌بیوتیک نورفلوکساسین از محلول آلوده بوسیله ریزجلبک کلرلا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

2 استادیار، گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

3 استادیار،گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

4 دانشیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

چکیده

در این پژوهش توانایی حذف آنتی­بیوتیک نورفلوکساسین از محلول آبی توسط ریزجلبک کلرلا مورد ارزیابی قرار گرفت. همچنین پیامدهای سمی این آنتی­بیوتیک بر روی ریزجلبک کلرلا با مطالعه ویژگی­های رشدی و بیوشیمیایی آن شامل وزن خشک سلول، کلروفیل کل و محتوای کارتنوئید بررسی شد. آزمایش در قالب طرح کاملاً تصادفی با آرایش فاکتوریل و با سه تکرار انجام شد. فاکتورهای آزمایش شامل پنج سطح آنتی­بیوتیک (صفر، 20، 50، 80، 120 میلی­گرم بر لیتر) و شش زمان نمونه­برداری (صفر، 1، 3، 6، 9 و ۱۴ روز) بود. وزن خشک سلولی ریزجلبک با افزایش غلظت نورفلوکساسین به طور معنی­داری کاهش یافت و در پایان ۱۴ روز انکوباسیون در غلظت­های ۵۰، ۸۰ و ۱۲۰میلی­گرم بر لیتر آنتی­بیوتیک در مقایسه با شاهد به ترتیب ۶1/21، 41/42 و 23/55 درصد کاهش نشان داد. مقادیر کلروفیل کل و کارتنوئید با افزایش زمان انکوباسیون به طور معنی­داری به دلیل تنش ناشی از نورفلوکساسین افزایش پیدا کردند. غلظت مؤثر (EC50) نورفلوکساسین برای ریزجلبک کلرلا 71/453 میلی­گرم بر لیتر در پایان 14 روز انکوباسیون محاسبه شد. کلرلا توانست پس از دو هفته انکوباسیون به ترتیب 15/69، 78/51، 41/28 و 18/18 درصد آنتی­بیوتیک نورفلوکساسین را از محلول آبی دارای غلظت­های 20، 50، 80 و 120 میلی­گرم بر لیتر حذف کند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Removal of Norfloxacin Antibiotic from Contaminated Solution by Microalgae Chlorella sp.

نویسندگان [English]

  • Zahra Darabi 1
  • Nasrin Ghorbanzadeh 2
  • Mohammad Bagher Farhangi 3
  • Davood Bakhshi 4
1 M.Sc. Student. Department of Soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Assistant Professor, Department of soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3 Assistant Professor, Department of Soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
4 Associate Professor, Department of Horticulture Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
چکیده [English]

In this study, the removal potential of norfloxacin antibiotic from water was investigated using Chlorella sp. microalga. Also, the toxicological effects of norfloxacin on Chlorella sp. was examined by studying the growth and biochemical characteristics of the microalga including cell dry weight, total chlorophyll and carotenoid content. A multi-level factorial experiment with complete randomized design was performed with three replications. The experimental factors included 5 levels of antibiotic (0, 20, 50, 80 and 120 mg L-1) and 6 sampling times (0, 1, 3, 6, 9 and 14 day). The cell dry weight of Chlorella sp. was significantly reduced at increased concentrations of norfloxacin, showing 12.61, 42.41 and 55.23% reduction at 50, 80 and 120 mg L−1 norfloxacin, respectively, as compared to the control treatment after 14 days of incubation. The total chlorophyll and carotenoid contents were significantly increased by prolonging incubation time as a result of norfloxacine stress. The effective concentration (EC50) of norfloxacin was calculated to be 453.71 mg L−1 for Chlorella sp. at the end of day 14. Chlorella sp. was able to eliminate norfloxacine up to 69.15, 51.78, 28.41 and 18.18% from 20, 50, 80 and 120 mg L−1 water solutions, respectively, after 14 days of incubation.

کلیدواژه‌ها [English]

  • Emerging contaminants
  • Algea
  • Bioaccumulation
  • Fluoroquinolone

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تحقیقات آب و خاک ایران
دوره 50، شماره 6
آبان 1398
صفحه 1307-1316

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