پیامد رسوب‌گذاری زیستی کربنات کلسیم بر آب‌شویی باکتری اشریشیا کولی در ستون شنی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 علوم و مهندسی خاک، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

2 گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

3 گروه علوم و مهندسی خاک، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

4 دانشیار گروه خاکشناسى، دانشکده کشاورزى، دانشگاه گیلان، رشت

چکیده

پیش­بینی انتقال باکتری­ها به آب­های زیرزمینی برای جلوگیری از آلودگی منابع آب به باکتری­های کلی­فرم بسیار حائز اهمیت است. این پژوهش با هدف بررسی کارایی روش رسوب­گذاری زیستی کربنات کلسیم (MICCP)‌ در جلوگیری از انتقال باکتری اشریشیا کولی به عنوان باکتری شناساگر آلودگی زیستی انجام شده است. برای انجام فرایند MICCP نمونه شن در لوله PVC (قطر داخلی 8/4 و بلندی 92/14 سانتی­متر) ریخته شد و برای سه روز همراه با باکتری اسپوروسارسینا پاستئوری انکوباسیون شد. پس از پیدایش جریان ماندگار اشباع، به اندازه 1/0 حجم منفذی از سوسپانسیون باکتری اشریشیا کولی (108 CFU/mL) به ستون افزوده شد و آب­شویی با آب مقطر انجام شد. از زه­آب خروجی از ستون­ها در حجم­های منفذی 1/0 تا 5 نمونه­برداری شد. پس از پایان آب­شویی، ستون شن به 5 برش 3 سانتی­متری تقسیم شد و شمار باکتری­های به دام افتاده در ستون اندازه­گیری شدند. شمارش باکتری­ها در محیط کشت EMB و به روش شمارش کلنی انجام شد. ستون شن کنترل نیز آب­شویی شد. پیامد تیمار MICCP و برهم­کنش آن با حجم منفذی بر شمار  اشریشیا کولی در زه­آب معنی­دار شد (05/0>p). پیامد عمق و برهم­کنش آن با تیمار نیز بر شمار ­مانده باکتری اشریشیا کولی در ستون و کربنات کلسیم معنی­دار بود (05/0>p). روی­هم­رفته، کارکرد کربنات کلسیم فراهم شده زیستی در پالایش باکتری­ها به ویژه در لایه­های بالایی ستون قابل توجه بوده و با کاهش ضریب آبگذری ستون شن، غلظت باکتری اشریشیا کولی در زه­آب کاهش یافت.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effect of Calcium Carbonate Bioprecipitation on Escherichia coli Leaching in Sand Column

نویسندگان [English]

  • Reyhaneh Shokati 1
  • Nasrin Ghorbanzadeh 2
  • Mohammad Bagher Farhangi 3
  • Mahmoud Shabanpour 4
1 Department of Soil Science, University of Guilan, Rasht, Iran
2 Department of soil Science, University of Guilan, Rasht, Iran
3 Department of Soil Science, University of Guilan, Rasht, Iran
4 Associate Professor, Department of Soil Science, University of Guilan, Rasht, Iran
چکیده [English]

Prediction of bacteria transport to groundwater is very important to prevent water resources from coliform bacteria pollusion. The objective of this study was to investigate the effectiveness of microbially induced calcium carbonate precipitation (MICCP) in preventing the transport of Escherichia coli as an indicator coliform. For the MICCP process, the sand sample was poured into PVC tubes (with inside diameter of 4.8 and height of 14.92 cm) and incubated for 3 days in the presence of Sporosarcina pasteurii. After the stablishment of steady-state flow, 0.1 pore volume of Escherichia coli suspension (108 CFU/mL) was added to the sand column and the leaching was followed with distilled water. The column effluent was sampled in 0.1 to 5 pore volume. After leaching, the sand column was sliced into five sections (~3 cm), and the number of E. coli trapped in each slice was measured using the plate count method in EMB agar culture medium. Leaching was also carried out in control sand column. The effect of MICCP treatment and its interaction with pore volume was significant (p<0.05) on Escherichia coli count in effluent. The effect of depth and its interaction with MICCP treatment were significant (p<0.05) on E. coli residuals and bioprecipitated calcium carbonate in the column. Overall, the role of biopreciptated calcium carbonate was significant in bacteria filtration, as it decreased the sand column hydraulic conductivity and reduced the number of E. coli in column effluents.

کلیدواژه‌ها [English]

  • Biomineralization
  • Urease
  • Hydraulic conductivity
  • Sporosarcina pasteurii
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