بررسی تجزیه زیستی BTEX توسط Bacillus thuringiensis و Bacillus sp. در شرایط کاهشی نیترات

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه مهندسی آبیاری و آبادانی، دانشکده مهندسی و فناوری کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

2 استاد، گروه مهندسی آبیاری و آبادانی، دانشکده مهندسی و فناوری کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

3 دانشیار، گروه مهندسی آبیاری و آبادانی، دانشکده مهندسی و فناوری کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

4 دانشیار، گروه مهندسی علوم خاک، دانشکده مهندسی و فناوری کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

چکیده

در محیط‌هایی با کمبود اکسیژن و نسبتاً شور تجزیه هیدروکربن‌های نفتی به سختی صورت می‌گیرد که با استفاده از پذیرنده‌های الکترون مانند نیترات و کاربرد باکتری‌های تجزیه‌گر و قادر به رشد در محیط‌های شور، سعی در افزایش تجزیه زیستی هیدروکربن‌ها می‌شود.  این مطالعه با هدف بررسی تجزیه زیستی BTEX با استفاده ازsp.  Bacillus وBacillus thuringiensisتحت شرایط کاهشی نیترات در محیط آئروفیلیک و شور انجام شد. در ابتدا توانایی باکتری‌های فوق در تجزیه زیستی BTEX مورد تائید قرار گرفت. سپس با استفاده از مدل تاگوچی و نرم‌افزار Design Expert تجزیه BTEX در شرایط محیطی مختلف با تغییر در عواملی مانند غلظت اولیه نیترات (500-100 میلی‌گرم بر لیتر)، BTEX (200-800 میلی‌گرم بر لیتر)، شوری (5/9- 5/0درصد) و جمعیت سلولی (107×5 – 1سلول در میلی‌لیتر) بررسی شد. نتایج حاصل از تحقیق توانایی Bacillusthuringiensis وsp. Bacillus رادر تجزیه زیستی BTEX نشان داد و شرایط بهینه را به‌منظور حداکثر تجزیه زیستی BTEX برای این دو باکتری‌ به ترتیب در غلظت اولیه BTEX برابر 200 میلی‌گرم بر لیتر، غلظت اولیه نیترات500 میلی‌گرم بر لیتر، شوری 5 و 5/9 درصد و جمعیت سلولی 1و 107×5 گزارش نمود.‌

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Assessment of the BTEX biodegradation by Bacillus thuringiensis and Bacillus sp. under nitrate reducing condition.

نویسندگان [English]

  • Mina Shakiba 1
  • Teymour Sohrabi 2
  • Farhad Mirzaei 3
  • Ahmad Ali Pourbabaee 4
1 PhD Student, Irrigation and Reclamation Engineering Department, Faculty of Agricultural Engineering and Technology, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Irrigation and Reclamation Engineering Department, Faculty of Agricultural Engineering and Technology, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professor, Irrigation and Reclamation Engineering Department, Faculty of Agricultural Engineering and Technology, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 Associate Professor, Soil Sciences Engineering Department, Faculty of Agricultural Engineering and Technology, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

Lack of oxygen and high salinity are factors that limit the biodegradation of the hydrocarbon. The electron accepters such as nitrate and salt tolerant bacteria can be used to increase the biodegradation rate in saline environment with low level of oxygen. In this paper, BTEX biodegradation by Bacillus thuringiensis andBacillus sp. is investigated in microaerophilic and saline environment under nitrate reducing condition. Firstly, the ability of the above- mentioned bacteria in BTEX biodegradation was confirmed. Then, the effect of Nitrate, BTEX concentration, salinity and cell mass on BTEX degradation was studied using Taguchi method and design expert software. The results of this study showed the ability of the Bacillus thuringiensis andBacillus sp. on BTEX degradation. For 200 mg/L BTEX concentration, the high efficiency of degradation by Bacillus thuringiensis andBacillus sp. could be achieved in optimum conditions; Nitrate Concentration (200 mg/L), salinity (1 and 5 %) and cell mass (1and 5×107 cell/ml) respectively.

کلیدواژه‌ها [English]

  • biodegradation
  • BTEX
  • Nitrate
  • salinity
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