بررسی شرایط بهینه حذف Zn+2 با استفاده از زیست‌توده باکتری‌های جداسازی شده ازمعدن راونج

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار گروه زمین شناسی، دانشگاه پیام‌نور، تهران

2 استادیار گروه محیط زیست، دانشکده منابع طبیعی، دانشگاه زابل

3 استادیار گروه مهندسی شیمی، دانشکده انرژی، دانشگاه صنعتی کرمانشاه

4 دانش‌آموختۀ کارشناسی ارشد بیوسیستماتیک جانوری، دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی

چکیده

آلودگی محیط به فلزات سنگین از مهم­ترین معضلات زیست‌محیطی بوده و منجر به آسیب‌های جدی بر سلامت انسان می­شود. به منظور کاهش اثرات زیان‌بار این فلزات باید روش‌های تصفیه آن­ها توسعه یابد که در این بین استفاده از جاذب­های زیستی اهمیت ویژه‌ای دارد. هدف از این مطالعه جداسازی باکتری‌های مقاوم به  از معدن سرب و روی راونج و شناسایی کارآمدترین سویه برای جذب روی بود. بدین منظور از رسوبات معدن، نمونه‌برداری انجام شد و باکتری‌های مقاوم به  غنی­سازی و خالص­سازی شدند. پس از تعیین مقاومت جدایه­های خالص‌شده به  ، کارامدترین جدایه شناسایی شد. پس از آن زیست‌توده خشک سویه خالص‌شده تهیه و تأثیر متغیرهای اصلی عملیاتی شامل  محیط، نسبت غلظت فلز روی به زیست‌توده باکتری()  و زمان‌ماند زیست‌توده باکتری در محیط حاوی روی ()  بر میزان حذف  با استفاده از روش سطح پاسخ و مدل Box-Behnken  ارزیابی و  بهینه‌سازی عددی مدل برای رسیدن به بیشترین مقدار حذف  از محیط آبی انجام شد. بر اساس نتایج بدست آمده از بین جدایه­های خالص‌شده، سویه MS3  Delftia lacustris  بیش­ترین توانایی را در تحمل  نشان داد (1200 میلی‌گرم بر لیتر). ماکزیمم درصد حذف  بر پایه مدل درجه دوم، در   (یعنی )،  (یعنی نسبت غلظت  به زیست‌توده باکتری ) و  (یعنی  ) بدست آمد. حداکثر میزان جذب در آزمایش­های انجام شده بر پایه طراحی آزمایشات 860/9 %، بود و در شرایط تحت مدل 490/9 % پیش‌بینی شد که دقت بالای مدل طراحی‌شده را نشان می‌دهد. بنابراین می­توان از سویه MS3 در جذب روی، به عنوان یک جاذب زیستی مؤثر در تصفیه زیستی فلزات سنگین استفاده نمود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Study of the Optimal Conditions for 〖Zn〗^(+2) Removal Using the Biomass of Isolated Bacteria from Ravang Mine

نویسندگان [English]

  • mehdi safari 1
  • mohsen shahriari moghadam 2
  • Mohsen Samimi 3
  • zahra azizi 4
1 Assistant Professor of Department of Geology, Payame Noor University, Tehran, Iran
2 Department of Environment, Faculty of Natural Resources, University of Zabol, Zabol, Iran
3 Assistant professor of Department of Chemical Engineering, Faculty of Energy, Kermanshah University of Technology, Kermanshah, Iran
4 Graduate Student (MSc) of Animal Biosystematics, Faculty of Biological Sciences and Technology, Shahid Behshti University, Tehran
چکیده [English]

Environmental pollution consist of heavy metals is the most important environmental problems and leads to serious damage for human health. In order to reduce the harmful effects of heavy metals, their treatment methods should be developed, of which the use of biological absorbers are particularly important. The objective of this study was to isolate the -resistant bacteria from Ravanj lead- and zinc-Mine in Markazi Province and to find the most efficient strains for zinc adsorption. For this purpose, samples were collected from the mine sediments and the  resistant bacteria were enriched in the medium and isolated. After determining the resistance of isolated bacteria to , the most effective strain (MS3, Delftia lacustris) were detected by 16S rDNA sequencing. Then after the dried strains biomass was prepared and the effect of main operational variables such as, Zinc to Bacteria Biomass concentration ratio (), and the retention time of bacteria biomass in the Zinc medium on Zinc removal has been evaluated and analyzed using the response surface method and Box-Behnken model. A numerical optimization model was performed to obtain the maximum amount of Zinc removal from aqueous solution. Among the isolated strains, the MS3 (Delftia lacustris) was the most tolerance strain to the Zinc (1200 mg/l). The maximum percentage of Zinc removal based on the quadratic model was obtained at (means), (means Zinc to Bacteria Biomass concentration ratio of), and  (means). The maximum amount of Zinc removal percentages based on the experimental design and the simulated model were 9.86% and 9.49% respectively, indicating the high accuracy of the model. Therefore, the MS3 strains can be used as a bio-absorbent for Zinc removal. 

کلیدواژه‌ها [English]

  • Zinc removal
  • biosorption
  • bactria
  • Optimization
  • Response surface

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تحقیقات آب و خاک ایران
دوره 50، شماره 1
فروردین و اردیبهشت 1398
صفحه 149-160

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