تاثیر لوتئولین، شوری و ترشحات بذر بر بیان ژن گره‌زایی Rhizobium meliloti L. با استفاده از ارزیابی فعالیت آنزیم β-galactosidase

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران

2 گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

چکیده

فلاونوئیدها نقش مهمی را به عنوان سیگنال‌های مولکولی در تشکیل گره­ها توسط ریزوبیوم­های همزیست بازی می­کنند. لوتئولین یکی از مهمترین فلاونوئیدها است که توسط بذرهای در حال جوانه­زنی یونجه ترشح می­شود. همچنین به نظر می­رسد سطح شوری بالا بدون حضور فلاونوئید موجب افزایش بیان ژن­های گره­زایی می­شود. بنابراین، در این آزمایش تأثیر لوتئولین، ترشحات بذر و شوری بر بیان ژن گره­زایی دو سویه حساس و مقاوم به شوری S. meliloti با استفاده از پلاسمید حامل پروموتور nodA و ژن lacZ از باکتری Escherichiacoli و از طریق فعالیت آنزیم β-galactosidase مورد بررسی قرار گرفت. نتایج نشان داد که پیش تیمار باکتریها با لوتئولین و ترشحات بذر بیان ژن nodA را افزایش داد که تأثیر ترشحات بذر بر بیان ژن بیشتر از لوتئولین بود. همچنین نتایج نشان داد که سطح شوری بالاmM  300 و 400 بدون حضور فلاونوئید منجر به بیان ژن گره­زایی شد و در شرایطی که شوری بالا و القاکننده­ها به طور همزمان وجود داشت، بیان ژن nodA بیشتر بود. به نظر می­رسد ریزوبیوم­ها از یک مکانیسم جایگزین برای بهبود گره­زایی در شرایط تنش استفاده می­کنند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of luteolin, seed exudate and salinity on the nod gene expression of R. meliloti using β-galactosidase activity assay

نویسندگان [English]

  • roghaye mardani 1
  • Kazem Poustini 2
  • AliReza Abbasi 2
  • AhmadAli Pourbabaei 2
1 1Department of Agronomy and Plant Breeding, University of Tehran
2 Department of Agronomy and Plant Breeding, University of Tehran
چکیده [English]

Flavonoids play a crucial role as signal molecules in promoting the formation of nodules by symbiotic bacteria commonly known as rhizobia. Luteolin is one of the most important flavonoids, which release from seeds during the first 4 hours of imbibition. It also seems that high NaCl concentrations induce the nod genes the absence of flavonoid inducers. We studied the effect of luteolin, Seed exudate and salinity on the induction of nod genes in R. Meliloti carrying a plasmid with a translational fusion between R. Meliloti nodA and lacZ of Escherichia coli, and the expression activity was measured by β -galactosidase activity. The results showed that pretreatment of bacteria with luteolin and seed exudate increased the expression of the nodA gene, that the effect of seed exudate on gene expression was more than the effect of luteolin. The results also showed that high salinityو 300 and 400 mM led to the expression nod gene in the absence of flavonoids and when there is high NaCl and inducers simultaneously, the expression of nod gene was greater. It seems that, rhizobium use a possible mechanism to improve nodulation under stress conditions.Due to increasing salinity and synthetic fertilizers problem, it seems searching about salinity and symbiosis of rhizobium and legums is importan and necessary.

کلیدواژه‌ها [English]

  • flavonoids
  • inducer
  • lacZ gene
  • nodA promoter
  • plasmid

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