جداسازی و شناسایی سیانوباکترها از خاک‌های فوق شور پارک ملی کویر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه تهران

2 دانشگاه الزهرا

چکیده

سیانوباکترها یک گروه منحصر به فرد از باکتری­های فتواتوتروف هستند که برخی از آن‌ها به دلیل ویژگی­های ساختاری، تحمل قابل توجهی به تنش شوری نشان می­دهند. این موجودات نقش مهمی در محیط­های خاکی به ویژه در نواحی خشک و نیمه­خشک ایفا می­کنند. در مطالعه حاضر سیانوباکترهای خاک از مناطق بیابانی ایران جداسازی شده و سپس جدایه­های مقاوم به شرایط فوق شور شناسایی شدند. 40 نمونه خاک از پارک ملی کویر تهیه شد. نمونه­ها پس از کشت در محیط BG11 و ASN III (5/3، 5، 6 و 7 درصد کلرید سدیم) و انکوباسیون در شرایط مناسب دمایی و نور، جداسازی شده و با استفاده از کلیدهای مورفولوژیکی به طور اولیه و سپس با روش مولکولی مورد شناسایی قرار گرفتند. از مجموع 40 مکان نمونه­برداری فقط از 4 مکان سویه­های سیانوباکتر مقاوم به شرایط فوق شور جداسازی شد. در محیط کشت فاقد کلرید سدیم 27 مورفوتایپ شناسایی شد که پنج سویه متعلق به دو جنس قادر به رشد در حضور 5/3 درصد کلرید سدیم بودند. در میان این پنج سویه، سه سویه در غلظت 5 و دو سویه در غلظت 6 درصد کلرید سدیم رشد کردند. تنها سویه Phormidium autumnale 61et قادر به تحمل غلظت 7 درصد کلرید سدیم بود که از مکانی با شوری 80/87 دسی زیمنس بر متر جداسازی شده بود. نتایج نشان داد که برخی از سیانوباکترهای جدا شده از خاک پارک ملی کویر قادرند در شرایط سخت مانند تنش شوری رشد کنند و توده بیولوژیکی فعالی را ایجاد نمایند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Isolation and identification of cyanobacteria from Kavir National Park hypersaline soils

نویسندگان [English]

  • Atefeh Etemadi-Khah 1
  • AhmadAli Poorbabaei 1
  • Hoseinali Alikhani 1
  • Mostafa Noroozi 2
1 University of Tehran
2 Ahzahra University
چکیده [English]

Cyanobacteria are a unique group of photoautotrophic bacteria that some of them due to their structural characteristics show a significant tolerance to salinity. These organisms play an important role in terrestrial environments, especially in arid and semi-arid region. In this study, soil cyanobacteria were isolated from the desert regions of Iran and then isolates resistant to hypersaline conditions identified. 40 soil samples were collected from the Kavir National Park. Samples were separated after culture in BG11 and ASN III (3.5, 5, 6 and 7% of NaCl) mediums and incubated under appropriate conditions of temperature and light and identified initially using morphological keys and then by molecular methods. Out of 40 sampling sites cyanobacteria were isolated only from four hypersaline sites. Twenty-seven morphotypes were identified in medium without sodium chloride that five strains belonging to two genera were able to grow in the presence of 3.5% sodium chloride. Among the five strains, three strains in 5% and two strains in 6% of sodium chloride grew. Only Phormidium autumnale 61et was able to tolerate 7% sodium chloride that was isolated from the site with 87.80 dS/m. The results showed that some isolated cyanobacteria from soils of Kavir National Park can grow in extreme conditions such as salinity stress and create a biologically active mass.

کلیدواژه‌ها [English]

  • 16S rRNA
  • Cyanobacteria
  • Desert
  • soil
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