ارزیابی روش‌های عصاره‌گیری تعیین آهن قابل‌استفاده گیاه ذرت در خاک‌های آهکی استان آذربایجان شرقی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد

2 دانشیار گروه علوم و مهندسی خاک دانشگاه تبریز

چکیده

این تحقیق برای ارزیابی روش­های عصاره­گیری آهن قابل‌استفاده گیاه ذرت در خاک و اندازه‌گیری آهن فعال گیاه کشت شده در 21 نوع خاک آهکی سطحی از استان آذربایجان شرقی انجام گرفت. در یک آزمایش گلخانه­ای در خاک‌های مورد مطالعه با سه تکرار گیاه ذرت (Zea mays L.) رقم سینگل کراس 704 کشت شد. پس از 60 روز شاخساره و ریشه ذرت برداشت شد. نتایج نشان داد که‌ آهن عصاره‌گیری شده از خاک توسط روش‌های DTPA و AB-DTPA بالاترین ضریب همبستگی را با شاخص­های رشد ذرت مانند وزن تر و خشک، غلظت آهن فعال در شاخساره، شاخص کلروفیل برگ‌ها، مقدار جذب آهن شاخساره و برخی ویژگی­های فیزیکی و شیمیایی خاک‌ها داشتند. روش AB-DTPA به‌دلیل ضریب همبستگی بالاتر نسبت به روش DTPA و عصاره­گیری هم‌زمان چندین عنصر، به‌عنوان عصاره­گیر برتر انتخاب شد. به‌طور میانگین AC-EDTA کمترین مقدار و روش اگزالات آمونیوم سریع بیشترین مقدار آهن را از خاک استخراج کردند. برای اندازه­گیری غلظت آهن فعال گیاه ذرت هر دو روش ارتوفنانترولین %5/1 و HCl یک نرمال مناسب تشخیص داده شدند. بین آهن فعال اندازه­گیری شده توسط ارتوفنانترولین با آهن فعال اندازه­گیری شده توسط HCl ضریب همبستگی خطی معنی­دار مشاهده شد (**66/0 r =، 01/0p<)، اما روش ارتوفنانترولین به دلیل همبستگی نزدیک‌تر با شاخص­های رشد گیاه و آهن قابل‌استفاده خاک عصاره­گیری شده با روش‌های DTPA و AB-DTPA نسبت به روش HCl برتر شناخته شد

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of Some Extraction for Determination of Corn Available Iron in Some Soils of East Azerbaijan Province

نویسندگان [English]

  • Kamal Khalkhal 1
  • Adel Reyhanitabar 2
  • Nosratollah Najafi 2
1
2
چکیده [English]

This research was conducted to evaluation of extraction methods for determining the available iron (Fe) and active Fe in corn plant (Zea mays L.) at 21 calcareous surface soil samples in the East Azerbaijan province. In a greenhouse experiment, corn plant single cross 704 cultivar has been cultivated with 3 replications. After 60 days, the plant growth parameters were measured. According to the results, DTPA and AB-DTPA had the highest linear correlation coefficient with growth indices of corn such as active Fe concentration, chlorophyll index, Fe content, fresh weight and shoot dry weight as well as with some physical and chemical properties of soils. AB-DTPA due to a correlation coefficient greater than DTPA and simultaneous extraction of multiple nutrients was chosen as the best extractant. On the average, rapid ammonium oxalate and AC-EDTA extracted the maximum and minimum amounts of Fe, respectively. The both of 1.5% o-phenanthroline and 1N HCl methods were suitable for measuring corn active Fe concentration. Significant correlation (r=0.66 ,p<0.01) was observed between the active Fe measured by o-phenanthroline and HCl , but o-phenanthroline method compared to HCl due to better correlation with growth indices and the extractable-Fe by DTPA and AB-DTPA methods was found to be superior.

کلیدواژه‌ها [English]

  • AB-DTPA
  • Corn
  • Calcareous soils
  • DTPA
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