بررسی اثرات بیوچار و اسید ‌سالیسیلیک بر کاهش تنش سرب در گیاه ریحان (Ocimum basilicum L)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد گروه علوم و مهندسی خاک دانشگاه رازی، کرمانشاه، ایران

2 استادیار گروه علوم و مهندسی خاک دانشگاه رازی، کرمانشاه، ایران

3 دانشیار گروه زراعت و اصلاح نباتات دانشکده کشاورزی دانشگاه رازی، کرمانشاه، ایران

چکیده

در این تحقیق، اثرات متقابل تنش سرب، اسید­ سالیسیلیک و بیوچار بر ویژگی­های رشدی ریحان (Ocimum basilicum L.) بررسی شد. آزمایش به صورت فاکتوریل، در قالب طرح کاملاٌ تصادفی با سه تکرار، در گلخانه دانشگاه رازی انجام گرفت. تیمارها شامل بیوچار کلزا در سه سطح (0، 1 و 3 درصد وزنی)، ­اسید سالیسیلک در سه سطح (0، 100 و 200 میکرومولار) و سرب در سه سطح (0، 100 و 200 میلی­گرم بر کیلوگرم از نمک نیترات سرب) بودند. نتایج نشان داد که بیشترین ارتفاع بوته، قطرساقه، وزن تر و خشک اندام هوایی، سطح برگ، وزن تر و خشک ریشه و حجم ریشه در تیمار 200 میکرومولار اسید سالیسیلیک، سه درصد بیوچار و شاهد سرب دیده شد. همچنین، کاربرد همزمان اسید ­سالیسیلیک و بیوچار، غلظت سرب اندام هوایی در گیاه ریحان را کاهش داد، بطوری­که کمترین غلظت سرب اندام هوایی (67/0 میلی­گرم بر کیلوگرم) در تیمار 200 میکرومولار اسید ­سالیسیلیک، سه درصد بیوچار و شاهد سرب مشاهده شد. در مقابل، بیشترین مقدار پرولین بخش هوایی (4/44 میلی­مول بر گرم) در تیمار شاهد اسید سالیسیلیک و بیوچار و سطح 200 میلی­گرم بر کیلوگرم سرب به دست آمد. بطورکلی، کاربرد اسید سالیسیلیک و بیوچار توانست بطور قابل ملاحظه­ای اثرات تنش سرب بر ویژگی­های رشدی ریحان را کاهش دهد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effects of Biochar and Salicylic Acid on Reducing Pb-induced Stress in Basil Crop (Ocimum basilicum L.)

نویسندگان [English]

  • Khadijeh Feyzi 1
  • Ali Asshraf Amirinejad 2
  • Mokhtar Ghobadi 3
1 Former MSc student, Department of Soil Science and Engineering, Razi University, Kermanshah, Iran
2 Assistant professor, Department of soil Science and Engineering, Razi University, Kermanshah, Iran
3 Associate professor, Agronomy Department, College of Agriculture, Razi University, Kermanshah, Iran
چکیده [English]

In this research, the interaction of Pb stress, salicylic acid (SA) and biochar (BC) on growth parameters of basil crop (Ocimum basilicum L.) were investigated. This experiment was conducted as a factorial, based on a complete randomized design with three replications in greenhouse of Razi University. Treatments included canola biochar at three levels (0, 1 and 3% by weight), SA at three levels (0, 100 and 200 μM) and Pb at three levels (0, 100 and 200 mg/kg Pb(NO3)2). Results showed that the highest plant height, stem diameter, fresh and dry plant weight, leaf area, fresh and dry root weight, and root volume were observed in the treatment of 200 μM SA, 3% BC and Pb control. Also, the simultaneous application of SA and BC reduced Pb concentration in basil, so that the lowest one (0.67 mg/kg) was found in the treatment of 200 μM SA, 3% BC and Pb control. In contrast, the highest proline content (44.4 mmol/g) was obtained in 200 mg/kg of Pb and control of SA and BC. In general, the application of SA and BC could significantly reduce Pb-induced stress on growth characteristics in basil.

کلیدواژه‌ها [English]

  • growth characteristics
  • Heavy metals
  • Medicinal Herb
  • proline
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