جداسازی و استفاده از باکتری‌های تولیدکننده اوره‌آز و ال-آسپاراژیناز موثر در تولید زیستی کربنات کلسیم به منظور حذف روی از محلول‌های آلوده

نوع مقاله : مقاله پژوهشی

نویسندگان

1 ﮔﺮوه ﻋﻠﻮم و ﻣﻬﻨﺪﺳﯽ ﺧﺎک، داﻧﺸﮑﺪه ﮐﺸﺎورزی، داﻧﺸﮕﺎه ﮔﯿﻼن، رﺷﺖ، اﯾﺮان

2 استادیار ﮔﺮوه ﻋﻠﻮم و ﻣﻬﻨﺪﺳﯽ ﺧﺎک، داﻧﺸﮑﺪه علوم ﮐﺸﺎورزی، داﻧﺸﮕﺎه ﮔﯿﻼن، رشت، ایران

3 ﮔﺮوه ﻋﻠﻮم و ﻣﻬﻨﺪﺳﯽ ﺧﺎک، داﻧﺸﮑﺪه ﮐﺸﺎورزی، داﻧﺸﮕﺎه ﮔﯿﻼن، رشت، ایران

چکیده

آلودگی منابع خاک و آب به فلزهای سنگین نه تنها در تولید محصولات کشاورزی سالم بلکه در سلامت اکوسیستم نیز به یک مسئله جدی تبدیل شده است. فرایند رسوب کربنات کلسیم تحریک شده میکروبی یک روش کم­هزینه و سازگار با محیط زیست در راستای کاهش آلودگی منابع آب و خاک است. هدف این پژوهش جداسازی باکتری­های بومی و موثر در تولید زیستی کربنات کلسیم به منظور حذف فلز روی از محلول­های آلوده بود. غربال­گری و جداسازی باکتری­های بومی تولیدکننده اوره­آز و ال-آسپاراژیناز انجام شد و سپس تغییرات آمونیاک، pH و قابلیت هدایت الکتریکی و همچنین حذف روی از محلول آلوده با کاربرد دو باکتری جدا شده در حضور باکتری شاخص Sporosarcinapasteuriiمورد مطالعه قرار گرفت. نتایج نشان داد که در حضور هر سه باکتری مقدار آمونیاک تولید شده، pH و قابلیت هدایت الکتریکی نسبت به شاهد (بدون مایه­زنی باکتری) افزایش معنی­داری پیدا کرد (p ≤0.01). کارایی جدایه تولید­کننده اوره­آز جدا شده در حذف روی از محلول آلوده تقریباً به اندازه کارایی باکتری Sporosarcinapasteuriiبود اما کارایی جدایه تولیدکننده ال-آسپاراژیناز بومی بالاتر از آن­ها بود. Sporosarcinapasteuriiحذف 32/51، 94/65، 36/70 درصدی و جدایه تولید­کننده اوره­آز حذف 49/65 و 07/68 و 46/71 درصدی را نسبت به مقدار اولیه روی به­ترتیب در غلظت­های 5/0، 2 و 4 میلی­مولار نشان دادند و جدایه تولیدکننده ال-آسپاراژیناز در غلظت­های 5/0، 2، 4 و 8 میلی­مولار روی به­ترتیب 29/96، 88/93، 06/97 و 32/97 درصد روی را حذف نمود. بنابراین به نظر می­رسد باکتری­های بومی تولیدکننده اوره­آز و ال-آسپاراژیناز می­توانند در زیست­پالایی روی از محیط­های آبی آلوده با فرایند رسوب کربنات کلسیم تحریک شده میکروبی مفید و کارآمد باشند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Isolating and Using Bacteria, Producing Urease and L-asparaginase, and Effective on Calcium Carbonate Bioproduction to Remove Zinc from Contaminated Solutions

نویسندگان [English]

  • Zahra Ghanbari 1
  • Nasrin Ghorbanzadeh 2
  • Mohammad Bagher Farhangi 1
  • Maryam Khalili Rad 3
1 Department of Soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Assistant Professor, Department of Soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3 Department of Soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
چکیده [English]

Heavy metal pollution in soil and water resources has become a serious problem not only in the production of healthy agricultural products, but also in the ecosystem health. Microbially induced calciumcarbonate precipitation (MICP) is a low-cost and environmentally friendly methods for reducing water resources and soil pollution. The aim of this study was to isolate native and efficient bacteria in the biological production of calciumcarbonate in order to remove zinc from contaminated solution. Isolating and screening native bacteria, producing urease and L-asparaginase, was accomplished.  Then, the changes in ammonia, pH and electrical conductivity (EC), as well as removal of zinc from the contaminated solutions were studied using these two efficient isolated bacteria in the presence of sporocarsina pasteurii. The results showed that in the presence of all three bacteria, the amount of produced ammonia, pH and EC in the culture media increased significantly compared to the ones in the control (without bacterial inoculation) (p < /em>≤0.05). The efficiency of isolated urease-producing strain in removal of zinc from the contaminated solution was almost equal to that of sporosarcina pasteurii, while the efficiency of isolated L-asparaginase-producing strain was more. Sporosarsina pasteurii removed 51.32, 65.94 and 70.36% and urease producing strain removed 65.49, 68.07, and 71.46 of zinc in the solutions containing 0.5, 2 and 4 mM Zn, respectively. However, L-asparaginase-producing strain removed 96.29, 93.88, 97.06 and 97.32% of zinc in solution containing 0.5, 2, 4 and 8 mM Zn, respectively. Therefore, it seems native urease- and L-asparaginase-producing bacteria can be useful and efficient in Zn bioremediation of contaminated solutions by MICP process.

کلیدواژه‌ها [English]

  • Biomineralization
  • bioremediation
  • Calcite
  • Heavy metals
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