تأثیر کاربرد هیومیک اسید و عنصر روی بر برخی ویژگی‌های رشد و آنزیم‌های آنتی‌اکسیدانی گیاهچه ذرت تحت تنش شوری خاک

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم خاک، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران

2 استادیار گروه خاکشناسی دانشگاه شهید چمران اهواز

3 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران

چکیده

یکی از روش­های مقابله با آسیب­های اکسیداتیو گیاهان کشت‌شده در خاک­های شور، استفاده از روش­های تغذیه و افزایش مقاومت گیاهان در برابر تنش شوری است. هدف از انجام این پژوهش بررسی تأثیر کاربرد خاکی هیومیک اسید و سولفات روی بر برخی صفات گیاهچه ذرت تحت تنش شوری خاک بود. بدین منظور آزمایشی به صورت کرت­های خردشده در قالب بلوک­های کامل تصادفی با سه تکرار در گلخانه اجرا گردید. تیمارهای آزمایش شامل شوری (خاک غیرشور و شور به­ترتیب با قابلیت هدایت الکتریکی 2/2 و 8 دسی زیمنس بر متر) به­عنوان فاکتور اصلی و کاربرد 5 کیلوگرم بر هکتار هیومیک اسید [HA]، 40 کیلوگرم بر هکتار سولفات روی [Zn]، 5 کیلوگرم بر هکتار هیومیک اسید+40 کیلوگرم بر هکتار سولفات روی [HA+Zn] و شاهد [Co ] به­عنوان فاکتور فرعی بودند. نتایج نشان داد با افزایش شوری در خاک، ارتفاع بوته­، قطر ساقه، وزن تر و خشک ریشه و اندام هوایی و شاخص کلروفیل ­به­طور معنی‌داری کاهش یافت در حالی­که مقدار پرولین و فعالیت آنزیم­های کاتالاز و سوپراکسیددیسموتاز افزایش یافت. در بین تیمارهای موردبررسی، تیمار HA+Zn بیشترین تأثیر را در بهبود شاخص­های رشد گیاه چه داشت به‌طوری‌که کاربرد این تیمار باعث افزایش 31/18، 76/16، 27/58، 89/59، 62/62، 25/65 و 85/4 درصدی در مقدار پارامترهای ارتفاع ساقه، قطر ساقه، وزن تر و خشک اندام هوایی، وزن تر و خشک ریشه و شاخص کلروفیل نسبت به تیمار شاهد در خاک شور [C1] شد. بیشترین میزان فعالیت آنزیم­های کاتالاز و سوپراکسیددیسموتاز و مقدار پرولین در شرایط تنش­شوری و استفاده از تیمار [HA+Zn] مشاهده شد و همچنین تأثیر هیومیک­اسید نیز بیشتر از تأثیر استفاده از سولفات روی بود. بنابراین می­توان مصرف توأم هیومیک­اسید و سولفات روی را در جهت بهبود رشد گیاهچه ذرت در شرایط تنش شوری توصیه کرد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effect of Humic Acid and Zinc Application on Some Vegetative Traits and Anti-oxidant Enzymes of Corn Seedling under Salinity Stress

نویسندگان [English]

  • atefeh rashidifard 1
  • mostafa chorom 1
  • Mojtaba Norouzi Masir 2
  • habibolah roshanfekr 3
1 Ph.D Student of Soil Science, Department of Soil Science, Faculty of Agriculture, Shahid Chamran University, Ahvaz, Iran
2 Assistant Professor of Soil Science, Department of Soil Science, Faculty of Agriculture, Shahid Chamran University, Ahvaz, Iran
3 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz Ahvaz, Iran
چکیده [English]

One of the methods to decrease the oxidative damage caused by salinity is using the nutrition methods and increasing resistance of plants against salinity stress. The aim of this study was to investigate the effect of soil application of humic acid and zinc sulfat on some properties of corn seedlings under salinity stress. For this purpose, an experiment was conducted as split-plot using a randomized complete block design with three replications in the greenhouse. Experimental treatments were included soil salinity (at two levels included non-saline and saline soils with EC of 2.2 and 8 dSm-1, respectively) as the main factor and application of 5 kg­ha-1 humic acid [HA], 40 kg­ha-1 zinc sulfat  [Zn], 5 kg­ha-1 humic acid+40 kg­ha-1 zinc sulfate [HA+Zn] and control [Co] as sub-factor. Results showed that the stem height and diameter, wet and dry weight of root and shoot and chlorophyll index decreased significantly with increasing salinity in soil, while proline content, catalase and superoxide-dismutase activities increased. Among the studied treatments, HA+Zn treatment had the greatest effect on improvement of seedling growth parameters, so that the application of treatment increased the amount of stem height, stem diameter, wet and dry weight of shoot and root and chlorophyll index, 18.31, 16.76, 58.27, 59.89, 62.62, 65.25 and 4.85%, respectively compared to control treatment in saline soils. The highest level of catalase and superoxide dismutase activities and proline content were observed under salinity stress along with HA+Zn treatment, and the effect of humic acid was greater than the effect of zinc sulfate. Therefore, the combined use of humic acid and zinc was recommended to improve the growth of corn seedlings in stress salinity conditions.

کلیدواژه‌ها [English]

  • anti-oxidant enzymes
  • Corn Seedling
  • Humic Acid
  • Salinity stress
  • Zinc
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