تحقیقات آب و خاک ایران

تحقیقات آب و خاک ایران

ارزیابی پاسخ‌های رشدی و فیزیولوژیک برموداگراس در سامانه پالایش الکتروسینتیک–زیست‌پالایی خاک آلوده به نفت با منحنی سطح پاسخ

نوع مقاله : مقاله پژوهشی

نویسندگان
1 بخش علوم خاک، دانشکده کشاورزی، دانشگاه شیراز، ایران
2 دانشکده مهندسی پایدار و محیطزیست، دانشگاه ایالتی آریزونا، ایالات متحده آمریکا، آمریکا
3 دانشکده معدن و علوم زمین، دانشگاه نظربایف، آستانه، قزاقستان
10.22059/ijswr.2026.413084.670124
چکیده
آلودگی خاک به نفت خام از پیچیده‌ترین چالش‌های محیط‌زیستی و نیازمند بهره‌گیری از رویکردهای جدیده برای اصلاح خاک می‌باشد. در این پژوهش، یک سامانه چندفازی و هم‌افزا شامل الکتروسینتیک (ولتاژ الکتریکی در سطوح 0، 5/1 و 3 ولت بر سانتی‌متر)، گیاه‌پالایی با گیاه برموداگراس و زیست‌پالایی با کنسرسیوم باکتری–جلبک (باکتری سودوموناس فلئورسانس و جلبک کلرولا) و در سطوح رطوبتی 60، 80 و 100 درصد ظرفیت مزرعه طراحی و اجرا شد تا کارایی آنها در پالایش خاک آلوده به سطوح 5/1، 3 و 5/4 درصد وزنی نفت خام ارزیابی شود. برای دستیابی به شرایط بهینه و مدل‌سازی اثر تیمارها بر شاخص‌های رشد و فیزیولوژیک گیاه (وزن تر و وزن خشک، ارتفاع گیاه، مساحت برگ و شاخص سبزینگی) از روش منحنی سطح پاسخ (RSM) استفاده شد. تحلیل‌ها نشان داد تعامل بین شدت میدان الکتریکی، سطح آلودگی و ساختار زیستی کنسرسیوم، الگوی پیچیده‌ای از پاسخ‌های گیاهی را رقم می‌زند و مدل‌های به‌دست‌آمده دارای R² تنظیم و پیش‌بینی‌شده بالا و بین 82/0 تا 99/0 بودند. در شرایط بهینه تمامی شاخص‌های رشد و فیزیولوژیک با کاربرد سیستم تلفیقی الکتروسینتیک-زیست‌پالایی افزایش یافت و بیشترین بهبود در متغیرها مورد مطالعه در نمونه‌های حاوی کنسرسیوم جلبک–باکتری به عنوان مهم‌ترین جنبه نوآورانه این پژوهش مشاهده شد. بیشینه میزان وزن ‌تر و ‌خشک، شاخص سبزینگی، ارتفاع گیاه و مساحت برگ در اجرای ۲۲ (آلودگی نفتی 5/1 درصد، جریان الکتریکی 5/1 ولت بر سانتی‌متر، تیمار بیولوژیکی 3 درصد کنسرسیوم جلبک (کلرلا)-باکتری (سودوموناس فلورسنس)) و رطوبت 80 درصد ظرفیت مزرعه) مشاهده شد که به‌ترتیب برابر با 10/50 و 67/20 گرم در گلدان، 50/70 و 50/45 سانتی‌متر و 90/4 سانتیمتر مربع در گلدان بودند. نتایج نشان داد بهره‌گیری از سامانه یکپارچه الکتروسینتیک–زیستی–گیاهی در کنار روش‌های مدل‌سازی مانند RSM، می‌تواند رویکردی مؤثر و قابل توسعه برای مدیریت خاک‌های آلوده به نفت و افزایش تاب‌آوری گیاه در این خاک‌ها فراهم آورد.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Evaluation of Growth and Physiological Responses of Bermudagrass in an Electrokinetic Bioremediation System for Oil-Contaminated Soil with A Response Surface Curve

نویسندگان English

Raziyeh Daryaee 1
Ali Akbar Moosavi 1
Reza Ghasemi 1
Amin Mojiri 2
Masoud Riazi 3
1 Department of Soil Science, College of Agriculture, Shiraz University, Shiraz, Iran
2 School of Sustainable Engineering and The Built Environment, Arizona State University, USA.
3 School of Mining and Geosciences, Nazarbayev University, Astana, Kazakhstan
چکیده English

Crude oil contamination of soil is a complex environmental challenge requiring novel remediation approaches. This study designed a synergistic system combining electrokinetics (0, 1.5, and 3 V/cm), phytoremediation with Bermuda grass, and bioremediation using a bacterial–algal consortium (Pseudomonas fluorescens and Chlorella) at moisture levels of 60%, 80%, and 100% of field capacity. The system was evaluated for remediating soil contaminated with 1.5%, 3%, and 4.5% crude oil. Response Surface Methodology (RSM) was used to optimize and model treatment effects on plant growth indices (fresh and dry weight, height, leaf area, and greenness index). RSM analyses showed complex interactions between electric field intensity, contamination level, and the biological consortium, with models achieving high R² values (0.82–0.99). Under optimal conditions, all plant indices increased with the application of the integrated electrokinetic-bioremediation system, and the greatest improvement in the studied variables was observed in samples containing the algal–bacterial consortium, representing the innovative aspect of this research. The maximum values of fresh and dry weight, greenness index, plant height, and leaf area were observed in treatment No. 22 (1.5% crude oil contamination, electric current of 1.5 V cm-1, biological treatment with 3% algal (Chlorella)-bacterial (Pseudomonas fluorescens) consortium, and soil moisture at 80% of field capacity), reaching 50.10 and 20.67 g pot-1, 70.50 and 45.50 cm, and 4.90 cm² pot-1, respectively. The results demonstrated that employing an integrated electrokinetic–biological–phytoremediation system alongside modeling approaches (e.g., RSM) can provide an effective and scalable strategy for managing oil-contaminated soils and enhancing plant resilience in such environments.

کلیدواژه‌ها English

Field capacity moisture, Microbial–algal consortium bioremediation, Oil pollution, Phytoremediation, Response Surface Methodology

Introduction

Soil is a complex mixture of mineral materials, organic matter, gases, liquids, and living organisms that, through continuous interactions, sustain life on Earth. Given its foundational role in engineering and agriculture, and particularly in securing food for the rapidly growing global population, the soil is one of the most important natural resources. Consequently, protecting soil and preventing contamination have become critical global priorities. Over the past decade, environmental pollution from different contaminants has emerged as one of the most serious challenges for human society, necessitating the application of eco-friendly, innovative remediation approaches. A comprehensive evaluation of the multi-dimensional impacts of these methods on soil and plant characteristics, along with comparing their efficacies and establishing operational priorities, has not been sufficiently evaluated. Therefore, this research aimed to design and evaluate an integrated, synergistic system combining electrokinetics, phytoremediation, and bioremediation. The primary innovation was applying a dried, powdered microbial-algal consortium (Chlorella biomass with Pseudomonas fluorescens) rather than live algae, contributing to nutrient supply, soil structure improvement, and microbial activity support. For the first time in such a triple system, Response Surface Methodology (RSM) was employed to model and optimize operational conditions with a specific focus on plant growth and physiological indices. This study offers a novel, sustainable solution to one of the most challenging environmental issues and enhances understanding of complex remediation mechanisms in oil-contaminated soils.

Materials and Methods

This greenhouse study used Response Surface Methodology (RSM) with a Box-Behnken design, implemented in Design-Expert software version 11. The experimental treatments comprised three levels of crude oil contamination: 1.5%, 3%, and 4.5%wt. Electrical potential was applied at three levels: 0, 1.5, and 3 V cm-1 of soil. Biological treatments included three levels: 0%, 3% Pseudomonas fluorescens bacterium alone, and a 3% consortium of dried Chlorella algae biomass and Pseudomonas fluorescens bacterium. Additionally, three soil moisture levels of 60%, 80%, and 100% of field capacity (FC) were imposed. Bermuda grass (Cynodon dactylon) was cultivated in calcareous loam-textured soil. The effects of treatments on plant growth and physiological indices, including fresh and dry weights, plant height, leaf area, and greenness index, were investigated. Measurements were taken before harvest. Plant height and leaf area were measured using a ruler. Greenness index was directly measured on healthy and mature leaves using a SPAD-502 chlorophyll meter. Subsequently, plants were harvested, and the fresh weight of shoots was recorded. Subsamples of harvested shoots were dried in an oven at 65°C for 48 hours to determine shoot dry weight. Finally, modeling, optimization, and validation were performed using the RSM approach in the Design-Expert 11 software environment.

Results and discussion

RSM analysis revealed that the interaction between electrical field intensity, contamination level, and consortium composition creates a complex pattern of plant responses. All resulting models were statistically significant, exhibiting high adjusted and predicted R² values ranging from 0.82 to 0.99. Under optimal conditions, all examined plant growth and physiological indices increased with the application of the integrated electrokinetic-bioremediation system. The most significant improvements in growth variables were observed in samples containing the algae-bacteria consortium (dried Chlorella biomass with Pseudomonas fluorescens solution), which constitutes the primary innovative aspect of this study. The maximum values for the studied traits were observed in Run 22 (1.5% crude oil contamination, 1.5 V cm-1 electrical potential, 3% algae-bacteria consortium treatment, and 80% FC soil moisture). These values were 50.10 and 20.67 g pot-1 for shoot fresh and dry weights, 70.50 for greenness index, 45.50 cm for plant height, and 4.90 cm² pot-1 for leaf area. Optimization results demonstrated minimal deviations between experimental and predicted values, with percentage errors falling within acceptable limits. Consequently, the RSM model effectively predicts fresh and dry weights, greenness index, plant height, and leaf area of Bermuda grass by integrating electrical potential, biological agent type, and soil moisture at varying crude oil contamination levels. The integrated electrokinetic–bio–phytoremediation system, combined with the RSM modeling, provides an effective and scalable approach for managing oil-contaminated soils and enhancing plant resilience.

Conclusion

The findings demonstrated that the integrated electrokinetic-bioremediation system is an effective and sustainable approach for enhancing plant growth in oil-contaminated soils. The synergy between electrical current and biological treatment, particularly the algae-bacteria consortium, effectively mitigated the adverse effects of oil contamination and created favorable conditions for plant development. Combining physical and biological processes overcomes limitations inherent in each method when applied independently. The simultaneous improvement in plant growth and physiological indices indicated that the system increases biomass and resilience under stress while providing a conducive environment for phytoremediation. This study also revealed that soil moisture stress does not necessarily lead to a significant decrease in plant performance. In other words, the system maintained its efficiency even under water-stress conditions, strengthening operational stability in arid regions where water shortage is common. The RSM-based multi-phase modeling proved to be a precise tool for designing remediation systems while reducing laboratory costs. Future research should investigate long-term sustainability at field scales and examine efficacy with various organic pollutants and different plant species. In summary, these results revealed the significant potential of the electrokinetic-bioremediation system as a novel, eco-friendly, scalable approach for remediating oil-contaminated soils, providing a scientific basis for practical strategies in soil conservation and restoration.

Funding

The study was funded by the University of Shiraz, Iran and National Iranian South Oilfields Company (53-180-04/0005).

 

Authorship contribution

Conceptualization, Ali Akbar Moosavi, Razieyeh Daryaee, Reza Ghasemi, Masoud Riazi, and Amin Mojiri; methodology, Razieyeh Daryaee, Ali Akbar Moosavi, Reza Ghasemi, Masoud Riazi, and Amin Mojiri; software, Razieyeh Daryaee and Ali Akbar Moosavi; validation, Razieyeh Daryaee, Ali Akbar Moosavi, Reza Ghasemi, Masoud Riazi, and Amin Mojiri; formal analysis, Razieyeh Daryaee and Ali Akbar Moosavi; investigation, Razieyeh Daryaee, Ali Akbar Moosavi, Reza Ghasemi, Masoud Riazi, and Amin Mojiri; resources, Ali Akbar Moosavi, Reza Ghasemi, and Masoud Riazi; data curation, Razieyeh Daryaee and Ali Akbar Moosavi; writing—original draft preparation, Razieyeh Daryaee and Ali Akbar Moosavi; writing—review and editing, Ali Akbar Moosavi, Reza Ghasemi, Masoud Riazi, and Amin Mojiri; visualization, Razieyeh Daryaee, Ali Akbar Moosavi, Reza Ghasemi, Masoud Riazi, and Amin Mojiri; supervision, Ali Akbar Moosavi; project administration, Ali Akbar Moosavi; funding acquisition, Ali Akbar Moosavi and Masoud Riazi.

All authors have read and agreed to the published version of the manuscript

Declaration of Generative AI and AI-assisted technologies in the writing process

In preparing this work, the authors used Grammarly and ChatGPT for grammar checking, improving the English abstract, and formatting references. After using these tools/services, the authors reviewed and edited the content as needed and take full responsibility for the content of the publication.

Data availability statement

 All information and results supporting the findings of this study are presented within the article.

Acknowledgements

The authors sincerely thank Shiraz University and National Iranian South Oilfields Company (53-180-04/0005) for their financial and technical support. The authors would like to thank anonymous reviewers for their valuable suggestions in manuscript revision.

Ethical considerations

The authors avoided data fabrication, falsification, plagiarism, and any form of misconduct.

Conflict of interest

The authors declare no conflict of interest.

Adam, G., & Duncan, H. J. (2002). Influence of diesel fuel on seed germination. Environmental Pollution, 120(2), 363-370. https://doi.org/10.1016/S0269-7491(02)00119-7 
Agbogidi, O. M., & Eshegbeyi, O. F. (2006). Performance of Dacryodes edulis (Don. G. Lam H. J.) seeds and seedlings in a crude oil contaminated soil. Journal of Sustainable Forestry, 22(3-4), 1-13. 
Agbogidi, O. M., Eruotor, P. G., & Akparobi, S. O. (2007). Effects of crude oil levels on the growth of maize (Zea mays L.). American Journal of Food Technology, 2(6), 529–535. 
Akpobaro, A., & Adeyemi, O. (2026). Morphophysiological and redox modulation of Abelmoschus esculentus under graded crude oil soil contamination.‏ IKR Journal of Agriculture and Biosciences, 2(1), 59-66.
Alanazi, F., Almugbel, R., Maher, H. M., Alodaib, F. M., & Alzoman, N. Z. (2021). Determination of tetracycline, oxytetracycline, and chlortetracycline residues in seafood products of Saudi Arabia using high-performance liquid chromatography–photo diode array detection. Saudi Pharmaceutical Journal, 29(6), 566–575. https://doi.org/10.1016/j.jsps.2021.04.017
Alavi, E., Tajadod, G., Jafari Marandi, S., & Arbabian, S. (2023). Vicia faba seed: a bioindicator of phytotoxicity, genotoxicity, and cytotoxicity of light crude oil. Environmental Science and Pollution Research, 30(8), 21043-21051.‏
Alharbi, B. H., Pasha, M. J., Alhudhudi, A. H., & Alduwais, A. K. (2018). Assessment of soil contamination caused by underground fuel leakage from selected gas stations in Riyadh, Saudi Arabia. Soil and Sediment Contamination, 27, 674–691. https://doi.org/10.1080/15320383.2018.1503228 
Ali, M. H., Khan, M. I., Naveed, M., & Tanvir, M. A. (2023). Microbe-assisted rhizoremediation of hydrocarbons and growth promotion of chickpea plants in petroleum hydrocarbons-contaminated soil. Sustainability, 15(7), 6081.‏
Ali, M. H., Khan, M. I., Naveed, M., & Tanvir, M. A. (2024). Microbe-assisted rhizodegradation of hydrocarbons and growth enhancement of wheat plants in hydrocarbons contaminated soil. International Journal of Environmental Science and Technology, 21(3), 3169-3184.‏
Almeida, Â., Cotas, J., Pereira, L., & Carvalho, P. (2023). Potential role of Spirogyra sp. and Chlorella sp. in bioremediation of mine drainage: A review. Phycology, 3(1), 186–201.
Alves, A. R., Yin, Q., Oliveira, R. S., Silva, E. F., & Novo, L. A. (2022). Plant growth-promoting bacteria in phytoremediation of metal-polluted soils: Current knowledge and future directions. Science of the Total Environment, 838, Article 156435. https://doi.org/10.1016/j.scitotenv.2022.156435
Anigboro, A. A., & Tonukari, N. J. (2008). Effect of crude oil on invertase and amylase activities in cassava leaf extract and germinating cowpea seedlings. Asian Journal of Biological Sciences, 1, 56–60.
Askari Mehrabadi, M., Noori, M., Amini, F., & Beigi, F. (2011). Evaluation of germination, chlorophyll content, and growth of black locust (Robinia pseudoacacia L.) in response to crude oil pollution. Iranian Journal of Plant Biology, 3(7), 41–54. (in Persian)
Babich, O., Sukhikh, S., Larina, V., Kalashnikova, O., Kashirskikh, E., Prosekov, A., ... & Dolganyuk, V. (2022). Algae: Study of edible and biologically active fractions, their properties and applications. Plants, 11(6), Article 780.
Baker, J. M. (1970). The effects of oils on plants. Environmental Pollution, 1, 27-44. https://doi.org/10.1016/0013-9327(70)90004-2 
Bakina, L. G., Polyak, Y. M., Gerasimov, A. O., Mayachkina, N. V., Chugunova, M. V., Khomyakov, Y. V., & Vertebny, V. A. (2022). Mutual effects of crude oil and plants in contaminated soil: a field study. Environmental Geochemistry and Health, 44(1), 69-82.‏
Bezerra, M. A., Santelli, R. E., Oliveira, E. P., Villar, L. S., & Escaleira, L. A. (2008). Response surface methodology (RSM) as a tool for optimization in analytical chemistry. Talanta, 76(5), 965–977.
Chaineau, C. H., Morel, J. L., & Oudot, J. (1997). Phytotoxicity and plant uptake of fuel oil hydrocarbons. Journal of Environmental Quality, 26, 1478-1483. 
Chattopadhyay, P., Karthick, A., & Roy, B. (2019). A review on the application of chemical surfactant and surfactant foam for remediation of petroleum oil contaminated soil. Journal of Environmental Management, 243, 187–205.
Chaudhary, P., Xu, M., Ahamad, L., Chaudhary, A., Kumar, G., Adeleke, B. S., ... & Abou Fayssal, S. (2023). Application of synthetic consortia for improvement of soil fertility, pollution remediation, and agricultural productivity: A review. Agronomy, 13(3), Article 643.
Cherian, S., & Oliveira, M. M. (2005). Transgenic plants in phytoremediation: Recent advances and new possibilities. Environmental Science & Technology, 39(24), 9377–9390.
Clementina, O. A., Olusola, J. O., & Luqman, S. K. (2008). Effect of spent engine oil on the growth parameters and chlorophyll content of Corchorus olitorius L. Environment Systems and Decisions, 28(4), 446-450. https://doi.org/10.1007/s10669-008-9165-5 
Daryaee, R., Moosavi, A. A., Ghasemi, R., & Riazi, M. (2024). Review of the effects of oil pollutants on physicochemical and biological soil properties. In H. Sarma & S. Joshi (Eds.), Biotechnology of emerging microbes (pp. 263–297). Elsevier.
da Silva Correa, H., Blum, C. T., Galvão, F., & Maranho, L. T. (2022). Effects of oil contamination on plant growth and development: a review. Environmental Science and Pollution Research, 29(29), 43501-43515.‏
Duan, Q., Han, G., Cui, B., & Rennenberg, H. (2023). The effects of petroleum contaminated soils on the growth, gas exchange and antioxidative level of sea-buckthorn. Plant and Soil, 486(1), 535-550.‏
Erfanmanesh, M., & Afyuni, M. (2007). [Water, Soil and Environmental Pollution] (6th Ed.). Isfahan University of Technology Academic Center for Education, Culture and Research (ACECR) Press. (in Persian)
Etesami, H., & Maheshwari, D. K. (2018). Use of plant growth-promoting rhizobacteria (PGPRs) with multiple plant growth-promoting traits in stress agriculture: Action mechanisms and future prospects. Ecotoxicology and Environmental Safety, 156, 225–246.
Etim, E. E. (2012). Phytoremediation and its mechanisms: A review. International Journal of Environmental and Bioenergy, 2(3), 120–136.
Gavrilescu, M. (2022). Enhancing phytoremediation of soils polluted with heavy metals. Current Opinion in Biotechnology, 74, 21–31.
Gee, G. W., & Bauder, J. W. (1986). Particle-size analysis. In A. Klute (Ed.), Methods of soil analysis: Part 1—Physical and mineralogical methods (2nd ed., pp. 383–411). American Society of Agronomy.
Geraei, H., & Shokrkar, H. (2025). Investigation of the biological removal of nickel and copper ions from aqueous solutions using mixed microalgae. Biofuels, Bioproducts and Biorefining, 19(1), 18-33.‏
Gharib, F. A. E. L., Osama, K., Sattar, A. M. A. E., & Ahmed, E. Z. (2024). Impact of Chlorella vulgaris, Nannochloropsis salina, and Arthrospira platensis as bio-stimulants on common bean plant growth, yield, and antioxidant capacity. Scientific Reports, 14(1), 1398.
Ghoneim, S. S., Dessouky, S. S., Boubakeur, A., Elfaraskoury, A. A., Abou Sharaf, A. B., Mahmoud, K., ... & Darwish, M. M. (2021). Accurate insulating oil breakdown voltage model associated with different barrier effects. Processes, 9(4), Article 657.
Gidudu, B., & Chirwa, E. M. (2022). The role of pH, electrodes, surfactants, and electrolytes in electrokinetic remediation of contaminated soil. Molecules, 27(21), Article 7381.
Gidudu, B., & Chirwa, E. M. N. (2020). Biosurfactants as demulsification enhancers in bio-electrokinetic remediation of petroleum contaminated soil. Process Safety and Environmental Protection, 143, 332–339.
Gnanasundar, V. M., & Raj, R. A. (2021). Remediation of inorganic contaminants in soil using electrokinetics, phytoremediation techniques. Materials Today: Proceedings, 45, 950–956. https://doi.org/10.1016/j.matpr.2020.03.038
Gods' gift, O. E., & Fagade, O. E. (2016). Microalgal-bacterial consortium in polyaromatic hydrocarbon degradation of petroleum–based effluent. Journal of Bioremediation & Biodegradation, 7(4), Article 1000365.
Hamid, F. S., Ossai, I. C., Ahmed, A., & Hassan, A. (2020). Remediation of soil and water contaminated with petroleum hydrocarbon: A review. Environmental Technology & Innovation, 17, Article 100526.
Helmke, P. A., & Sparks, D. L. (1996). Lithium, sodium, potassium, rubidium, and cesium. In D. L. Sparks (Ed.), Methods of soil analysis: Part 3—Chemical methods (pp. 551–574). Soil Science Society of America.
Hewedy, A. M. (1999). Influence of single and multi-bacterial fertilizer on the growth and fruit yield of tomato. Egyptian Journal of Applied Science, 14(7), 508–523.
Hol, W. G., Bezemer, T. M., & Biere, A. (2013). Getting the ecology into interactions between plants and the plant growth-promoting bacterium Pseudomonas fluorescens. Frontiers in Plant Science, 4, Article 81.
Hosseini, S., Azadi, D., & Absalan, A. (2023). Screening and molecular identification of nocardia with ability to biodegradation of Polycyclic Aromatic Hydrocarbons and phenol from Iranian ecosystems. Journal of Cellular and Molecular Research (Iranian Journal of Biology), 36(2), 103-115. (in Persian)
Huang, H., Tang, J., Niu, Z., & Giesy, J. P. (2019). Interactions between electrokinetics and rhizoremediation on the remediation of crude oil-contaminated soil. Chemosphere, 229, 418-425. https://doi.org/10.1016/j.chemosphere.2019.04.150
Huang, X. D., El-Alawi, Y., Penrose, D. R., Glick, B., & Greenberg, B. (2004). Responses of three grass species to creosote during phytoremediation. Environmental Pollution, 130, 453-463.
Huang, X. D., El-Alawi, Y., Penrose, D. M., Glick, B. R., & Greenberg, B. M. (2004). A multi-process phytoremediation system for removal of polycyclic aromatic hydrocarbons from contaminated soils. Environmental Pollution, 130(3), 465–476.
Hu, J., Chen, J., Wang, W., & Zhu, L. (2023). Mechanism of growth inhibition mediated by disorder of chlorophyll metabolism in rice (Oryza sativa) under the stress of three polycyclic aromatic hydrocarbons. Chemosphere, 329, 138554.‏
Hussein, Z. S., Hamido, N., Hegazy, A. K., El-Dessouky, M. A., Mohamed, N. H., & Safwat, G. (2022). Phytoremediation of crude petroleum oil pollution: a review. Egyptian Journal of Botany, 62(3), 611-640.‏
Iqbal, S., Ummara, U., Noreen, S., Akhter, M. S., Jaleel, F., Jabeen, S., ... & Aqeel, M. (2024). Enhancing systematic tolerance in Bermuda grass (Cynodon dactylon L.) through amplified alkB gene expression and bacterial-driven hydrocarbon degradation. Environmental Science and Pollution Research, 31(13), 19871–19885.
Jeyasundar, P. G. S. A., Ali, A., Azeem, M., Li, Y., Guo, D., Sikdar, A., ... & Zhang, Z. (2021). Green remediation of toxic metals contaminated mining soil using bacterial consortium and Brassica juncea. Environmental Pollution, 277, Article 116789. https://doi.org/10.1016/j.envpol.2021.116789
Kamari, E., & Farshadfar, M. (2014). Novel phytoremediation technology for achieving a sustainable environment. Journal of Biosafety, 5(2), 107–121. (in Persian)
Keshavarz, S., Ghasemi-Fasaei, R., Ronaghi, A., & Mousavi, A. A. (2021). Innovative assisted phytoremediation of multi-elements contaminated soil by ryegrass: An electro-biochemical approach. Journal of Soils and Sediments, 21(7), 2604–2618.
Khan, S., Masoodi, T. H., Pala, N. A., Murtaza, S., Mugloo, J. A., Sofi, P. A., ... & Kumar, A. (2023). Phytoremediation prospects for restoration of contamination in the natural ecosystems. Water, 15(8), 1498.‏
Kouhkan, H., Golchin, A., Mortazavi, M. S., Shahriari, F., & Hemati, R. (2021). Simultaneous efficiency of phytoremediation and bioremediation in crude oil removal from soil. Journal of Water and Soil Conservation Research, 27(2), 25–45. (in Persian)
Kumar, A., Das, A., Singh, D., Das, M. K., Srivastava, G. P., Singh, J. P., ... & Chakdar, H. (2023). Soil health restoration in degraded lands: A microbiological perspective. Land Degradation & Development, 34(17), 5155-5170.‏
Lal, M. A., & Bhatla, S. C. (2023). Metabolism of storage carbohydrates. In Plant physiology, development and metabolism (pp. 231-259). Singapore: Springer Nature Singapore.‏
Li, J. H., Gao, Y., Wu, S. C., Cheung, K. C., Wang, X. R., & Wong, M. H. (2008). Physiological and biochemical responses of rice (Oryza sativa L.) to phenanthrene and pyrene. International Journal of Phytoremediation, 10(2), 106–118.
Li, M., Huang, Y., Li, K., Yuan, X., Liu, H., Li, M., ... & Xi, Y. (2023). Enhancement of electrokinetic-phytoremediation by Ophiopogon japonicus: stimulation of electrokinetic on root system and improvement of polycyclic aromatic hydrocarbon degradation. Environmental Science and Pollution Research, 30(43), 97591-97600. https://doi.org/10.1007/s11356-023-29342-7
Liu, L., Luo, D., Lu, Y., Huang, X., Liu, Y., Wei, L., ... & Liu, G. (2022). Risk assessment of groundwater pollution during GLDA-assisted phytoremediation of Cd- and Pb-contaminated soil. Ecological Indicators, 139, Article 108913.
Loeppert, R. H., & Suarez, D. L. (1996). Carbonate and gypsum. In D. L. Sparks (Ed.), Methods of soil analysis: Part 3—Chemical methods (pp. 437–474). Soil Science Society of America.
Lorestani, B., Kolahchi, N., Ghasemi, M., & Cheraghi, M. (2013). Effect of different oil concentrations on the growth parameters of Faba vulgaris. In Proceedings of the First National Conference on Plant Stresses (pp. 1–4). University of Isfahan. (in Persian)
Maila, M. P., & Cloete, T. E. (2002). Germination of Lepidium sativum as a method to evaluate polycyclic aromatic hydrocarbons (PAHs) removal from contaminated soil. International Biodeterioration & Biodegradation, 50(2), 107–113.
Malallah, G., Afzal, M., Kurian, M., Gulshan, S., & Dhami, M. S. I. (1998). Impact of oil pollution on some desert plants. Environment International, 24(8), 919–924.
Mandari, T., & Lin, J. (2007). Isolation and characterization of engine oil degrading indigenous microorganisms in wazulu-Natal, South Africa. African Journal of Biotechnology, 6(1), 23-27.
Manzar, M. S., Khan, G., dos Santos Lins, P. V., Zubair, M., Khan, S. U., Selvasembian, R., ... & Kayed, T. S. (2021). RSM-CCD optimization approach for the adsorptive removal of Eriochrome Black T from aqueous system using steel slag-based adsorbent: Characterization, isotherm, kinetic modeling, and thermodynamic analysis. Journal of Molecular Liquids, 339, Article 116714. https://doi.org/10.1016/j.molliq.2021.116714
Medina, V. F., Maestri, E., Marmiroli, M., Dietz, A. C., & McCutcheon, S. C. (2003). Plant tolerances to contaminants. In S. C. McCutcheon & J. L. Schnoor (Eds.), Phytoremediation: Transformation and control of contaminants (pp. 189–232). Wiley.
Meudec, A., Poupart, N., Dussauze, J., & Deslandes, E. (2007). Relationship between heavy fuel oil phytotoxicity and polycyclic aromatic hydrocarbon contamination in Salicornia fragilis. Science of the Total Environment, 381(1–3), 146–156.
Mohammadi, F., Samaei, M. R., Azhdarpoor, A., Teiri, H., Badeenezhad, A., & Rostami, S. (2019). Modelling and optimizing pyrene removal from the soil by phytoremediation using response surface methodology, artificial neural networks, and genetic algorithms. Chemosphere, 237, Article 124486. https://doi.org/10.1016/j.chemosphere.2019.124486
Mohrazi, A., Ghasemi-Fasaei, R., Mojiri, A., & Shirazi, S. S. (2023). Investigating an electro-bio-chemical phytoremediation of multi-metal polluted soil by maize and sunflower using RSM-based optimization methodology. Environmental and Experimental Botany, 211, Article 105352.
Muthukumaran, M. (2026). Indigenous microalgal biodiversity for efficient phycoremediation and biomass valorization of oil-drilling effluent. Journal of Water Process Engineering, 88, 110190.‏
Nemati, A., Golchin, A., & Ghavidel, A. (2019). Evaluating the effects of co-using phytoremediation and bioremediation in a crude oil contaminated soil. Water and Soil, 33(4), 579–590. https://doi.org/10.22067/jsw.v0i0.76812
Ogundele, O. D., Ogundele, D. T., Popoola, O. J., Adelekun, A. A., & Olagunju, V. A. (2025). Phytoremediation in wetlands. In Wetland Ecosystems: Conservation Strategies, Policy Management and Applications (pp. 199-237). Cham: Springer Nature Switzerland.‏
Olguín, E. J. (2003). Phycoremediation: Key issues for cost-effective nutrient removal processes. Biotechnology Advances, 22(1–2), 81–91.
Omosun, G., Markson, A. A., & Mbanasor, O. (2008). Growth and anatomy of Amaranthus hybridus as affected by different crude oil concentrations. *American-Eurasian Journal of Scientific Research, 3*(1), 70–74.
Otugboyega, J. O., & Adeyemi, O. (2025). Phytotoxic effects of crude oil contamination on maize growth parameters.‏ IKR Journal of Agriculture and Biosciences, 1(4), 174-188.
Palmroth, M. R. T., Pichtel, J., & Puhakka, J. A. (2002). Phytoremediation of subarctic soil contaminated with diesel fuel. Bioresource Technology, 84, 221-228.
Peretiemo-Clarke, B. O., & Achuba, F. I. (2007). Phytochemical effect of petroleum on peanut (Arachis hypogea) seedlings. Journal of Plant Pathology, 6(2), 179–182.
Pinna, M. V., Diquattro, S., Garau, M., Grottola, C. M., Giudicianni, P., Roggero, P. P., ... & Garau, G. (2024). Combining biochar and grass-legume mixture to improve the phytoremediation of soils contaminated with potentially toxic elements (PTEs). Heliyon, 10(5).‏
Polyak, Y. M., Bakina, L. G., Mayachkina, N. V., Chugunova, M. V., Bityutskii, N. P., Yakkonen, K. L., & Shavarda, A. L. (2024). Long-term effects of oil contamination on soil quality and metabolic function. Environmental Geochemistry and Health, 46(1), 13.‏
Priya, A. K., Muruganandam, M., Ali, S. S., & Kornaros, M. (2023). Clean-up of heavy metals from contaminated soil by phytoremediation: A multidisciplinary and eco-friendly approach. Toxics, 11(5), 422.‏
Rath, B. (2012). Microalgal bioremediation: Current practices and perspectives. Journal of Biochemical Technology, 3(3), 299–304.
Rathi, M., & Yogalakshmi, K. N. (2021). Brevundimonas diminuta MYS6 associated Helianthus annuus L. for enhanced copper phytoremediation. Chemosphere, 263, Article 128195.
Razmjoo, K., & Adavi, Z. (2012). Assessment of bermudagrass cultivars for phytoremediation of petroleum contaminated soils. International Journal of Phytoremediation, 14(1), 14–23.
Rhoades, J. D. (1996). Salinity: Electrical conductivity and total dissolved salts. In D. L. Sparks (Ed.), Methods of soil analysis: Part 3—Chemical methods (pp. 417–436). Soil Science Society of America.
Richard, S. L. A. (1954). Diagnosis and improvement of saline and alkaline soils (U.S. Salinity Laboratory Staff, Agriculture Handbook No. 60). U.S. Government Printing Office.
Richards, L. A., & Fireman, M. (1943). Pressure-plate apparatus for measuring moisture sorption and transmission by soils. Soil Science, 56(6), 395–404.
Rocha, I. M. V., Silva, K. N. O., Silva, D. R., Martínez-Huitle, C. A., & Santos, E. V. (2019). Coupling electrokinetic remediation with phytoremediation for depolluting soil with petroleum and the use of electrochemical technologies for treating the effluent generated. Separation and Purification Technology, 208, 194–200.
Rosso, P. H., Pushnik, J. C., Lay, M., & Ustin, S. L. (2005). Reflectance properties and physiological responses of Salicornia virginica to heavy metal and petroleum contamination. Environmental Pollution, 137(2), 241–252.
Ruiz-Marin, A., Canedo-Lopez, Y., Campos-Garcia, S. D. C., Sabido-Perez, M. Y., & Zavala-Loria, J. D. C. (2013). Biodegradation of wastewater pollutants by activated sludge coimmobilized with Scenedesmus obliquus. Agrociencia, 47(5), 429–441.
Saadati, N., Davatgar, N., Roodpeyma, M., Mosaddeghi, M. R., & Bostani, A. (2015). Evaluation of petroleum contamination effects on soil water repellency intensity in Bakhtiardasht of Isfahan. Iranian Journal of Soil Research, 29(3), 359-369. (in Persian)
Saini, A., Bekele, D. N., Chadalavada, S., Fang, C., & Naidu, R. (2021). Electrokinetic remediation of petroleum hydrocarbon contaminated soil (I). Environmental Technology & Innovation, 23, Article 101585.
Sakhaei, Z., Daryaee, R., Moosavi, A. A., Carrasco-Marin, F., Betancur, S., Bailón-García, E., Perez-Cadenas, A. F., & Riazi, M. (2024). Chemical-assisted biological methods for in situ remediation of petroleum hydrocarbon-contaminated soils. In H. Sarma & S. Joshi (Eds.), Biotechnology of emerging microbes (pp. 217–261). Elsevier.
Sharma, P. K., & Kumar, S. (2023). Soil air and plant growth. In Soil Physical Environment and Plant Growth: Evaluation and Management (pp. 155-174). Cham: Springer International Publishing.‏
Shen, X., Dai, M., Yang, J., Sun, L., Tan, X., Peng, C., ... & Naz, I. (2021). A critical review on the phytoremediation of heavy metals from the environment: Performance and challenges. Chemosphere, 291(3), Article 132979.
Sivasakthi, S., Usharani, G., & Saranraj, P. (2014). Biocontrol potentiality of plant growth promoting bacteria (PGPR) — Pseudomonas fluorescens and Bacillus subtilis: A review. African Journal of Agricultural Research, 9(16), 1265–1277.
Smit, B., Stachowiak, M., & Van Volkenburgh, E. (1989). Cellular processes limiting leaf growth in plants under hypoxic root stress. Journal of Experimental Botany, 40(1), 89–94.
Song, X., Li, C., & Chen, W. (2022). Phytoremediation potential of Bermuda grass (Cynodon dactylon L.) Pers.) in soils co-contaminated with polycyclic aromatic hydrocarbons and cadmium. Ecotoxicology and Environmental Safety, 234, Article 113389.
Subashchandrabose, S. R., Venkateswarlu, K., Venkidusamy, K., Palanisami, T., Naidu, R., & Megharaj, M. (2019). Bioremediation of soil long-term contaminated with PAHs by algal–bacterial synergy of Chlorella sp. MM3 and Rhodococcus wratislaviensis strain 9 in slurry phase. Science of the Total Environment, 659, 724–731.
Sule, B. T., Olawepo, K. G., & Najeemdeen, B. A. (2025). Impact of kolanut biochar on corchorus olitorius growthand nutritional value in cadmium-polluted soil. African Journal of Geographical Sciences, 7(1), 15-26.‏
Teiri, H., Hajizadeh, Y., Samaei, M. R., Pourzamani, H., & Mohammadi, F. (2020). Modelling the phytoremediation of formaldehyde from indoor air by Chamaedorea elegans using artificial intelligence, genetic algorithm, and response surface methodology. Journal of Environmental Chemical Engineering, 8(5), Article 104225.
Telo da Gama, J. (2023). The role of soils in sustainability, climate change, and ecosystem services: Challenges and opportunities. Ecologies, 4(3), 552-567.‏
Thomas, G. W. (1996). Soil pH and soil acidity. In D. L. Sparks (Ed.), Methods of soil analysis: Part 3—Chemical methods (pp. 475–490). Soil Science Society of America.
Tulkova, E. G., Savchenko, G. E., & Kabashnikova, L. F. (2022). Degradation of chlorophyll in the leaves of Reed Fescue (Festuca arundinacea) under the action of volatile organic compounds and benzo (a) pyrene. Biology Bulletin, 49(4), 282-291.‏
Ugbeni, O. C., Ebite, L. A., & Ikhelegbe-Ojo, D. (2022). Antioxidant enzyme activities of the leaves of Groundnut (Arachis hypogeae L.) planted on crude oil-contaminated soil. African Scientist, 23(4), 240.‏
Walkley, A., & Black, I. A. (1934). An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Science, 37, 29–38.
Wang, J., Liu, X., Zhang, X., Liang, X., & Zhang, W. (2011). Growth response and phytoremediation ability of reed for diesel contaminant. Laboratory of Environmental Remediation, College of Environmental and Chemical Engineering, Shanghai University, China, 8, 68-74.
Wang, Y., Li, Z., Wu, J., Liu, H., Sun, X., Liu, L., & Du, S. (2022). Abscisic acid-catabolizing bacteria: A useful tool for enhancing phytoremediation. Science of the Total Environment, 812, Article 151474.
Wang, Z., Gao, N., Li, Y., Song, C., Kang, E., Wang, S., ... & Shang, Z. (2025). A soil aeration system improved the growth of tomato plants and inhibited the occurrence of Fusarium root rot. Scientia Horticulturae, 349, 114235.‏
Wyszkowski, M., Wyszkowska, J., Kordala, N., & Borowik, A. (2022). Effects of coal and sewage sludge ashes on macronutrient content in maize (Zea mays L.) grown on soil contaminated with Eco-Diesel oil. Materials, 15(2), 525.‏
Yan, H., Zhang, T., Li, Y., Liang, X., He, Y., Zhan, F., ... & Li, Y. (2026). Intercropping Pennisetum giganteum and Medicago sativa for iron-manganese mitigation and control in coal gangue: root system dynamics and phytoremediation potential. Plant and Soil, 521(1), 619-637.‏
Zeb, B. S., Hayat, M. T., Zeb, T., Khan, F. Y., Abbasi, H. Z., Nawaz, I., & Ebadi, A. (2022). Uptake of organic pollutants and the effects on plants. In Sustainable plant nutrition under contaminated environments (pp. 209-234). Cham: Springer International Publishing.‏
Zeng, G., Tang, L., Ren, X., Wang, J., Wan, J., Wang, J., Deng, Y., Liu, Y., & Peng, B. (2018). The potential impact on the biodegradation of organic pollutants from composting technology for soil remediation. Waste Management, 72, 138–149.
Zhang, X., Chen, J., Wang, W., & Zhu, L. (2024). Photosynthetic mechanisms of carbon fixation reduction in rice by cadmium and polycyclic aromatic hydrocarbons. Environmental Pollution, 344, 123436.‏
دوره 57، شماره 8
چاپ شده در اول آبانماه 1405
آبان 1405
صفحه 1928-1956