Removal of Norfloxacin Antibiotic from Contaminated Solution by Microalgae Chlorella sp.

Document Type : Research Paper


1 M.Sc. Student. Department of Soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Assistant Professor, Department of soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Assistant Professor, Department of Soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

4 Associate Professor, Department of Horticulture Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran


In this study, the removal potential of norfloxacin antibiotic from water was investigated using Chlorella sp. microalga. Also, the toxicological effects of norfloxacin on Chlorella sp. was examined by studying the growth and biochemical characteristics of the microalga including cell dry weight, total chlorophyll and carotenoid content. A multi-level factorial experiment with complete randomized design was performed with three replications. The experimental factors included 5 levels of antibiotic (0, 20, 50, 80 and 120 mg L-1) and 6 sampling times (0, 1, 3, 6, 9 and 14 day). The cell dry weight of Chlorella sp. was significantly reduced at increased concentrations of norfloxacin, showing 12.61, 42.41 and 55.23% reduction at 50, 80 and 120 mg L1 norfloxacin, respectively, as compared to the control treatment after 14 days of incubation. The total chlorophyll and carotenoid contents were significantly increased by prolonging incubation time as a result of norfloxacine stress. The effective concentration (EC50) of norfloxacin was calculated to be 453.71 mg L1 for Chlorella sp. at the end of day 14. Chlorella sp. was able to eliminate norfloxacine up to 69.15, 51.78, 28.41 and 18.18% from 20, 50, 80 and 120 mg L1 water solutions, respectively, after 14 days of incubation.


Main Subjects

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