Improving the yield of button mushroom (Agaricus bisporus) by changing the chemical properties of casing soil under the influence of biochar application

Document Type : Research Paper

Authors

1 PhD. student Department of Soil Science, College of Agriculture, Shahrekord. University, Shahrekord, Iran

2 Associate Professor, Department of Soil Science, College of Agriculture, Shahrekord University, Shahrekord, Iran

3 Professor, Department of Soil Science, College of Agriculture, Shahrekord University, Shahrekord, Iran

4 Director of Development and Research of Negin Fasl Agro-Industry Company, Shahrekord, Iran

10.22059/ijswr.2025.395712.669947

Abstract

In Iran, natural peat casing soil resources are limited and its harvesting has many environmental consequences. This study aimed to investigate the use of biochar in casing soil composition and its effect on the chemical properties of casing soil and the yield of mushrooms cultivated in it as a factorial in a completely randomized design with 29 treatments in 2024. Accordingly, the initial casing soil compositions including north production peat, northern production peat vermicompost, cow manure vermicompost, and spent mushroom vermicompost were prepared. Then, 5, 10, and 15 percent of biochar was added to each composition and different treatments were prepared. After preparing the casing soil, treatments usage and cultivating the mushroom. The characteristics of the pH, EC, and OM of the soil and the mushroom yield were measured. The results of this study showed that in all the studied soils, with increasing biochar, the pH, EC, and OM of the casing soil increased, and the mushroom yield had a significant positive correlation with the pH and OM of the casing soil and a significant negative correlation with the EC. The overall results showed that treatments mixed with cow manure vermicompost were superior to other treatments in terms of lower salinity and high yield. The use of biochar is importance in increasing soil organic matter, and economically, among different levels of biochar, adding 5% by weight of biochar to the soil is effective in increasing organic matter, and high organic matter in the casing soil leads to improved mushroom yield.

Keywords

Main Subjects


Introduction

Casing soil is an important input in the production of the button mushroom, which is usually prepared from non-renewable organic sources (peat). This study was conducted to investigate the use of biochar in casing soil composition and its effect on the chemical properties of casing soil and the yield of cultivated mushrooms.

Method

This study was conducted in a completely randomized design with 29 treatments and three replications on an industrial scale in Negin Fasl Agro-industry. Accordingly, the initial soil casing compositions including north production peat, north production peat vermicompost, cow manure vermicompost, and SMC vermicompost were prepared. Then, biochar was added to each soil casing composition to prepare experimental treatments and different soil casing treatments were prepared.

Experimental design and treatment application

The experiment was conducted in a completely randomized factorial design with three factors: type of casing soil at four levels, biochar application rate at four levels, and biochar particle size at two levels, with three replications. A control treatment (the casing soil used in the mushroom production facility).

The casing soil type factor consisted of four levels (S1, S2, S3, and S4), which were respectively S1: mixture of north production peat and SMC vermicompost in a weight ratio of 50 percent, S2: mixture of north production peat and cow manure vermicompost in a weight ratio of 50 percent, S3: vermicompost mixture of north production peat and SMC in a weight ratio of 50 percent, and S4 :vermicompost mixture of north production peat and cow manure in a weight ratio of 50 percent. The biochar amount factor consisted of four levels L0, L1, L2 and L3 with values ​​of 0, 5, 10 and 15 Weight percent respectively and the biochar particle size factor consisted of two levels Z1 and Z2 (0-1 and 1-2 mm particles).

Measuring casing soil properties

In this study, immediately after Step of adding casing soil, a soil sample was taken from each experimental plot. The pH and EC were measured using a pH meter and EC meter after air-drying the samples and passing them through a 2 mm sieve. The organic matter content was determined by the dry combustion method.

Evaluation of the overall performance of button mushrooms

At each harvest stage, yield parameters of first-grade mushrooms (firm texture, closed cap, and good to excellent appearance) and second-grade mushrooms (semi-firm texture, open cap, and average appearance) were measured using a scale with 0.01 g precision. Total yield was calculated as the sum of the yields of first-grade and second-grade mushrooms.

Statistical analyses

Statistical analyses and determination of correlation coefficients between measured casing soil characteristics and button mushroom yield were performed using SPSS software.

Results

The effect of biochar application on casing soil pH showed an increasing trend in all four soil types S1, S2, S3 and S4, but this increase was not significant. In all studied soils, with increasing biochar content, casing soil EC increased. The highest electrical conductivity was associated with the S1l3z1 treatment with a value of 2.99 dS.m-1 and the lowest electrical conductivity was associated with the S4l2z1 treatment Which has not significant difference with most treatments containing cow manure vermicompost. Therefore, casing soil with lower electrical conductivity was more effective in increasing yield with biochar application. Also, the highest average organic matter content was obtained in the S4l1z1 treatment (60.31%) and the lowest organic matter content was obtained in the S0 treatment (21.84%). The high soil organic matter content led to improved mushroom performance.

Conclusions

Overall, the results indicated that the highest EC values were observed in treatments containing soil S3, while the lowest EC values were found in treatments with soil S2. Therefore, the mixtures containing vermicompost from cow manure exhibited lower soil salinity and higher yield compared to the commercial casing soil and other treatments. The application of biochar in the casing soil mixture can play a significant role in increasing the soil organic matter content. Economically, among the different biochar levels tested, the addition of 5% (w/w) biochar to the soil was effective in enhancing organic matter. Increased organic matter in the casing soil.

Author Contributions

  For this research article, the individual contributions are as follows: Conceptualization, [Author A] and [Author B], [Author C] and [Author D]; methodology, [Author B]; software, [Author C]; validation, [Author A], [Author B], and [Author C]; formal analysis, [Author A] and [Author D]; investigation, [Author B]; resources, [Author A]; data curation, [Author B]; writing original draft preparation, [Author C]; writing review and editing, [Author B]; visualization, [Author A]; supervision, [Author B]; project administration, [Author C]; funding acquisition ,[Author A]. All authors have read and agreed to the published version of the manuscript.

Data Availability Statement

The data that support the findings of this study are available. For further inquiries regarding the data, please contact author’s email.

Acknowledgements

 The authors would like to thank the reviewers and editor for their critical comments that helped to improve the paper.

Ethical considerations

The authors avoided data fabrication, falsification, plagiarism, and misconduct.

Conflict of interest

The author declares no conflict of interest.

Introduction

Casing soil is an important input in the production of the button mushroom, which is usually prepared from non-renewable organic sources (peat). This study was conducted to investigate the use of biochar in casing soil composition and its effect on the chemical properties of casing soil and the yield of cultivated mushrooms.

Method

This study was conducted in a completely randomized design with 29 treatments and three replications on an industrial scale in Negin Fasl Agro-industry. Accordingly, the initial soil casing compositions including north production peat, north production peat vermicompost, cow manure vermicompost, and SMC vermicompost were prepared. Then, biochar was added to each soil casing composition to prepare experimental treatments and different soil casing treatments were prepared.

Experimental design and treatment application

The experiment was conducted in a completely randomized factorial design with three factors: type of casing soil at four levels, biochar application rate at four levels, and biochar particle size at two levels, with three replications. A control treatment (the casing soil used in the mushroom production facility).
The casing soil type factor consisted of four levels (S1, S2, S3, and S4), which were respectively S1: mixture of north production peat and SMC vermicompost in a weight ratio of 50 percent, S2: mixture of north production peat and cow manure vermicompost in a weight ratio of 50 percent, S3: vermicompost mixture of north production peat and SMC in a weight ratio of 50 percent, and S4 :vermicompost mixture of north production peat and cow manure in a weight ratio of 50 percent. The biochar amount factor consisted of four levels L0, L1, L2 and L3 with values ​​of 0, 5, 10 and 15 Weight percent respectively and the biochar particle size factor consisted of two levels Z1 and Z2 (0-1 and 1-2 mm particles).

Measuring casing soil properties

In this study, immediately after Step of adding casing soil, a soil sample was taken from each experimental plot. The pH and EC were measured using a pH meter and EC meter after air-drying the samples and passing them through a 2 mm sieve. The organic matter content was determined by the dry combustion method.

Evaluation of the overall performance of button mushrooms

At each harvest stage, yield parameters of first-grade mushrooms (firm texture, closed cap, and good to excellent appearance) and second-grade mushrooms (semi-firm texture, open cap, and average appearance) were measured using a scale with 0.01 g precision. Total yield was calculated as the sum of the yields of first-grade and second-grade mushrooms.

Statistical analyses

Statistical analyses and determination of correlation coefficients between measured casing soil characteristics and button mushroom yield were performed using SPSS software.

Results

The effect of biochar application on casing soil pH showed an increasing trend in all four soil types S1, S2, S3 and S4, but this increase was not significant. In all studied soils, with increasing biochar content, casing soil EC increased. The highest electrical conductivity was associated with the S1l3z1 treatment with a value of 2.99 dS.m-1 and the lowest electrical conductivity was associated with the S4l2z1 treatment Which has not significant difference with most treatments containing cow manure vermicompost. Therefore, casing soil with lower electrical conductivity was more effective in increasing yield with biochar application. Also, the highest average organic matter content was obtained in the S4l1z1 treatment (60.31%) and the lowest organic matter content was obtained in the S0 treatment (21.84%). The high soil organic matter content led to improved mushroom performance.

Conclusions

Overall, the results indicated that the highest EC values were observed in treatments containing soil S3, while the lowest EC values were found in treatments with soil S2. Therefore, the mixtures containing vermicompost from cow manure exhibited lower soil salinity and higher yield compared to the commercial casing soil and other treatments. The application of biochar in the casing soil mixture can play a significant role in increasing the soil organic matter content. Economically, among the different biochar levels tested, the addition of 5% (w/w) biochar to the soil was effective in enhancing organic matter. Increased organic matter in the casing soil.

Author Contributions

  For this research article, the individual contributions are as follows: Conceptualization, [Author A] and [Author B], [Author C] and [Author D]; methodology, [Author B]; software, [Author C]; validation, [Author A], [Author B], and [Author C]; formal analysis, [Author A] and [Author D]; investigation, [Author B]; resources, [Author A]; data curation, [Author B]; writing original draft preparation, [Author C]; writing review and editing, [Author B]; visualization, [Author A]; supervision, [Author B]; project administration, [Author C]; funding acquisition ,[Author A]. All authors have read and agreed to the published version of the manuscript.

Data Availability Statement

The data that support the findings of this study are available. For further inquiries regarding the data, please contact author’s email.

Acknowledgements

 The authors would like to thank the reviewers and editor for their critical comments that helped to improve the paper.

Ethical considerations

The authors avoided data fabrication, falsification, plagiarism, and misconduct.

Conflict of interest

The author declares no conflict of interest.