Document Type : Research Paper
Authors
1
Department of Agricultural Sciences, Faculty of Engineering, Islamic Azad University, Mashhad Branch, Mashhad< Iran
2
Department of Agricultural Science, Mashad Branch, Islamic Azad University, Mashhad, Iran.
3
Seed and Plant Improvement Department, Agricultural and Natural Resources Research and ‎Education Center of Khorasan-e Razavi, Agricultural-Research- Education & Extension ‎Organization (AREEO), Mashhad, Iran.
4
Department of Agricultural Science, Mashhad Branch, Islamic Azad University, Mashad, Iran
Abstract
In order to study the effect of deficit irrigation on RWC, proline concentration, antioxidant enzymes content, chlorophyll concentration and forage yield of five clover species an experiment was conducted in split plots arranged based on randomized complete block design with three replications at the Education-Research Farm, Mashhad Branch, Islamic Azad University, Mashhad, Iran, during the cropping season 2019-2020. Deficit irrigation at three levels (0 (Full irrigation), 20 and 40% less than the irrigation requirement) were considered as main plots and five clover species (Trifolium resupinatum ، T. alexandrium ، T. lapaceaum ، T. echinatum و T. diffusum) were allocated as sub plots.The results showed that with increasing of deficit irrigation (increasing stress), proline concentration and antioxidant enzymes content increased. However, RWC, concentration of chlorophyll a and b and yield of fresh and dry fodder decreased. The highest RWC, chlorophyll concentration and content of antioxidant enzymes were observed without significant difference in T. resupinatum and T. alexandrium species. The investigation of the effect of two factors showed that, with the increase of deficit irrigation, fresh and dry forage yield of all five species decreased significantly. In all three levels of deficit irrigation, In general, the results showed that all 5 species do not have the required stability under stress conditions. Therefore, applying the method of deficit irrigation in the studied species it will be justified only if a 20 and 40 percent reduction in the amount of water used for this product is more beneficial than the reduction in yield.
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