Comparison of structural diversity of humic acid types extracted from conventional organic sources in agriculture

Document Type : Research Paper

Authors

1 Soil Science and Engineering department, College of Agriculture, Razi university, Kermanshah, Iran

2 Soil Science and Engineering department, College of Agriculture, Razi University, Kermanshah, Iran

3 Soil Science and Engineering Department, College of Agriculture, Razi University, Kemanshah, Iran

Abstract

Humic acid is an organic compound that can improve the physical, chemical and biological properties of soil. In addition, it plays an important role in increasing plant growth and yield. This composition can be extracted from various sources that are rich in organic materials such as composts, coal, humus materials and soil. In order to know the spectral and structural characteristics of this compound, humic acid was extracted from different organic sources in 2021. The amounts of carbon, oxygen, hydrogen and nitrogen elements in the extracted humic acids were determined by elemental analyzer, functional groups by FTIR and the spectrophotometric absorption ratios of E3/E5 and E4/E6 were determined by spectrophotometer in Department of Soil Science and Engineering laboratory, Razi University, Kermanshah. The lowest H/C ratio in the extracted humic acids was observed from municipal waste compost (1.05) and vermicompost (1.09), the highest O/C ratio was in humic acid extracted from municipal waste compost (0.70) and vermicompost (0.59), and the lowest C/N ratio was in humic acid of chicken manure (11.84). According to the results of FTIR analysis, the strongest absorption bands were observed at 3400 cm-1, 2900-3000 cm-1, 1600- 1700 cm-1 and 1000-1100 cm-1. The highest ratio of E3/E5 was found in humic acid extracted from chicken manure (12.81) and sheep manure (10.61) and the lowest amount was observed in municipal waste compost (2.00). The highest ratio of E4/E6 was obtained in humic acid extracted from chicken manure (7.31) and the lowest amount of E4/E6 was observed in humic acid obtained from municipal waste compost (4.70). 

Keywords

Main Subjects

EXTENDED ABSTRACT

 

Introduction:

Humic acid is an organic compound that can improve the physical, chemical and biological properties of soil. In addition, it plays an important role in increasing plant growth and yield. This composition can be extracted from various sources that are rich in organic materials such as composts, coal, humus materials and soil.

In order to know the spectral and structural characteristics of this compound, humic acid was extracted from different organic sources in the Department of Soil Science and Engineering of Razi University, Kermanshah, Iran in 2021.

 

Materials & Methods

Two organic sources of cow manure compost and vermicompost were the result of the composting process in previous researches in the Faculty of Agriculture. Municipal waste compost was prepared from Kermanshah city waste recycling and compost production factory. Sheep and chicken manure were also obtained from the farm of the Faculty of Agriculture. The amounts of carbon, oxygen, hydrogen and nitrogen elements in the extracted humic acids were determined by elemental analyzer, functional groups by FTIR and the spectrophotometric absorption ratios of E3/E5 and E4/E6 were determined by spectrophotometer.

 

Results

The amount of carbon was between 47.61 and 57.67 %, hydrogen between 4.19 and 7.16 %, nitrogen between 3.30 and 5.68 %, and oxygen between 30.18 and 44.90 %. The lowest H/C ratio in the extracted humic acids was observed from municipal waste compost (1.05) and vermicompost (1.09), the highest O/C ratio was in humic acid extracted from municipal waste compost (0.70) and vermicompost (0.59), and the lowest C/N ratio was in humic acid of chicken manure (11.84). According to the results of FTIR analysis, the strongest absorption bands were observed at 3400 cm-1, 2900-3000 cm-1, 1600- 1700 cm-1 and 1000-1100 cm-1. The results of this part showed that the extracted humic acids had aromatic rings in addition to aliphatic compounds and included COOH, COO-, OH, C=O and C-O functional groups in their structure. The results of analysis of variance showed that there was a significant difference between the absorbance ratios of E3/E5 and E4/E6 in humic acids extracted from different sources at 1% level. The highest ratio of E3/E5 was found in humic acid extracted from chicken manure (12.81) and sheep manure (10.61) and the lowest amount was observed in municipal waste compost (2.00). The highest ratio of E4/E6 was obtained in humic acid extracted from chicken manure (7.31) and the lowest amount of E4/E6 was observed in humic acid obtained from municipal waste compost (4.70).

 

Conclusion

In general, humic acid extracted from municipal waste compost had higher degree of polymerization, aromatic compounds, degree of condensation and humification than the other organic sources, and humic acid obtained from chicken manure had the lowest degree of humification and decomposition.

 

Keywords: Elemental analysis E3/E5, E4/E6, FTIR, Humic acid

References

Abbt Braun, G. & Frimmel, F. H. (1999). Basic characterization of Norwegian NOM samples similarities and differences. Environment International, 25 (2/3), 161 –180.
Alikhani, H. & Hemati, A. (2014). Effect of Vermicompost Enrichment with Chemical Fertilizer and Bacterial Treatments on Humification and Acid Humic Properties. Journal of Agricultural Science and Sustainable Production, 24 (1), 113- 125. (In Persian)
Aranganathana, L., Radhika Rajasreea, S. R. Sumana, T. Y. Remyaa, R. R. Gayathria, S. Jayaseelana, C.,Karthiha, M. G. & Gobalakrishnana, M. (2019). Comparison of molecular characteristics of Type A humic acids derived from fish waste and sugarcane bagasse co-compost influenced by various alkaline extraction protocols. Microchemical Journal, 149, 1-8.
Behravan, H. R., Voroney, P. Khorassani, R. Fotovat, A. Moezei, A. A. & Taghavi, M. (2019). Chemical and Spectroscopic Characterization of Humic Acids Extracted from Filter Cake using Different Basic Solutions. Society for Sugar Research and Promotion. Sugar Tech, 1-8.
Chen, Y., Senesi, N. & Schnitzer, M. (1977). Information provided on humic substances by E4 /E6 ratios. Soil Science Society of American Journal, 41, 352 –358.
Demirbas, A., Yakup, K. & Huseyin, D. (2006). Humic Substances and Nitrogen-Containing Compounds from Low Rank Brown Coals, Energy Sources Part A: Recovery, Utilization, and Environmental Effects, 28 (4), 341-351.
Doskočil, L., Burdíková-Szewieczková, J. Enev, V. Kalina, L. & Wasserbauer, J. (2018). Spectral characterization and comparison of humic acids isolated from some European lignites. Fuel, 213,123–132.
Eshwar, M., Srilatha, M. Bhanu Rekha, K. & Harish Kumar Sharma, S. (2017). Characterization of humic substances by functional groups and spectroscopics methods. International Journal of Current Microbiology and Applied Sciences, 6 (10), 1768- 1774.
Fong, S.S., Seng, L., Chong, W.N., Asing, J., Faizal, M., Nor, M. & Mohd Pauzana, A.S. (2006). Characterization of the coal derived humic acids from Mukah, Sarawak as soil conditioner. Journal of the Brazilian Chemical Society, 17(3), 582–587.
Fuentes, M., Baigorri, R. Gonzalez-Gaitano, G. & Garcia-Mina, J. M. (2016). New methodology to assess the quantity and quality of humic substances in organic materials and commercial products for agriculture. Journal of Soil Sediments, 18, 1-11.
Giovanela, M., Crespo, J.S., Antunes, M., Adametti, D.S. & Fernandes, A.N. (2010). Chemical and spectroscopic characterization of humic acids extracted from the bottom sediments of a Brazilian subtropical microbasin. Journal of Molecular Structure, 981(1–3), 111–119.
Gondar, D., Lopez, R. Fiol, S. Antelo, J. M. & Arce, F. (2005). Characterization and acid–base properties of fulvic and humic acids isolated from two horizons of an ombrotrophic peat bog. Geoderma, 126, 367–374.
Gonzalez-vila., F. J. & Martin, F. (1985). Chemical strucral characteristics of humic acids extracted from composted municipal refuse. Agriculture, Ecosystems and Environment, 14, 267-278.
Hanc, A., Enev, V. Hrebeckova, T. Klucakova, M. & Pekar, M. (2019). Characterization of humic acids in a continuous-feeding vermicomposting system with horse manure. Wast Management, 99, 1–11.
Hoseini, S., Hejazi Mehrizi, M. Sarcheshmehpour, M. & Fekri, M. (2017). Comparison of some spectrophotometric ratios of humic acids extracted from plant residues compost and organic fertilizers. 15 th of Soil Science Congress of Iran. Isfahan University of Technology. (In Persian)
Ibrahim, M. I. M., Awad, E. A. M. Dahdouh, S.M.M. El- Etr,W. M. T. & Ibrahim, A. S. M. (2019). Characterization of some organic materials sources and analysis of the humic acids extracted from them. Zagazig Journal of Agricultural Research, 46 (3), 685- 698.
Kononova, M. M. (1966). Soil organic matter. 2nd ed. Pergamon, Oxford, UK.
Kumada, K. (1965). Studies on the colour of humic acids: part 1 on the concepts of humic substances and humification. Soil Science and Plant Nutrition, 11, 1116.
Kumada, K. (1987). Chemistry of soil organic matter, first ed., Japan Scientific Societies Press, Tokyo, 1987, p. 241.
Kumada, K. & Kawamura, Y. (1968). Viscos metric characteristics of humic acid. Soil Science and Plant Nutrition, 14, 190–197.
Li, X., Xing, M. Yang, J. & Huang, Z. (2011). Compositional and functional features of humic acid-like fractions from vermicomposting of sewage sludge and cow dung. Journal of Hazardous Materials, 18, 740–748.
Malakouti, M. J. (2014). Optimum ferilizer consumption recommendation for Agricultural Products in Iran. Mobaleghan Publisher. Pages, 348. (In Persian).
Omar, M. M., Taha A. A. & Abbas, A. E. (2018). Physicochemical characteristics of humic and fulvic acids extracted from different feedstocks. Soils Dept. Fac. of Agric., Mansoura Univ., Egypt. 9 (7), 277 – 281.
Page, A. L. 1982. Methods of soil analysis. Agronomi 9, ASA, SSSA, Madison, Wiscosin, USA.
Qi, B. C., Aldrich, C. and Lorenzen, L. (2004). Effect of ultra-sonication on the humic acids extracted from lignocellulose substrate decomposed by anaerobic digestion. Chemical Engineering Journal, 98, 153-163
Rajashekhar, D., Srilantha, M. Chandrasekhar Rao, P. Harish Kumar Sharma, S. & Bhanu Rekha, K. (2017). Functional and spectral characterization of humic fractions obtained from organic manures. International Journal of Pure and Applied Bioscience, 5 (6), 1254- 1259.
Sanchez-Monedero, M. A., Roig, A. Cegarra, J. Bernal, M. P. & Paredes, C. (2002). Effects of HCl-HF purification treatment on chemical composition and structure of humic acids. European Journal of Soil Science, 53, 375-381.
Sarlaki, A., Sharif pa ghaleh, A. Kianmehr, M. H. & Shakiba, N. (2017). Spectral, structural and chemical study of humic acids extracted from coals in Iranian mines. Iranian Journal of Soil and Water Research, 48 (5), 1145- 1158. (In Persian)
Shahbazi, K., Marezi, M. & Tabakhian, SH. (2019). The evaluation of methods for determination of humic and fulvic acids in fertilizer materials. Journal of Land Management, 7 (1), 97-114. (In Persian)
Swift, R. S. (1996). Organic matter characterization. In Methods of soil analysis, part 3: Chemical methods, ed. D. L. Sparks, Madison, Wisc.Soil Science Society of America, 1011–1069.
Tale Farahi, F. (2014). Investigation of humic acids extracted from sludge, biochar and animal manure on sunflower yield in calcareous soil. MSc Thesis of Soil Science. Zabol University. (In Persian)
Wali, A., Ben Salah, I. Zerrouki, M. Choukchou-Braham, A. Kamoun, Y. & Ksibi, M. (2019) .A novel humic acid extraction procedure from Tunisian lignite. Euro-Mediterranean Journal for Environmental Integration, 4 (24), 1-9.
Weber, J.W., McGinley, P.M. & Katz, L. E. (1992). A distributed reactivity model for sorption by soils and sediments. 1. Conceptual basis and equilibrium assessments. Environmental Science & Technology, 26, 1955 – 1962.
Iranian Journal of Soil and Water Research
Volume 54, Issue 1
April 2023
Pages 123-134

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