Investigation of the phytoremidation of lead in the metallicolous and non- metallicolous species Matthiola.

Document Type : Research Paper


1 Department of Biology, Faculty of Science , Payame Noor University, Tehran, Iran

2 Department of Biology, Faculty of Science and Technology, University of Isfahan, Isfahan , Iran


Lead is one of the most abundant and toxic heavy metals which can have lethal effects on plants. The Pb contamination is produced by mining, industrial, activities and fossil fuel burning. Some metallophytes plants belong to Brassicaceae family are able to grow in soils contaminated with heavy metals. The purpose of this study is to evaluate the resistance and lead accumulation of the metallicolous species Matthiola flavida collected from the vicinity of the Irankouh Pb/Zn mine in Isfahan, Iran, which is compared with its non- metallicolous species Matthiola incana to used Pb phytoremediation. Non-metallicolous (Matthiola incana) and metallicolous plants (Matthiola flavida) were transferred to hydroponic mediums and after proper vegetative growth, they were exposed to 0, 10, 50, 100, 150 mg/L treatments of lead for 14 days. The results showed by increasing lead concentration, the growth of both species significantly decreased, but this reduction in growth was always greater in the non-metallicolous species, so that at the highest stress level, the dry weight of shoots and roots decreased in the metallicolous to %7.1 and % 28.8, but in the non-metallicolous to %69.9 and %60.8 in comparison with their control, respectively. With increasing the concentration of Pb in the medium, the accumulation of lead in the roots of both species are enhanced, but Pb concentration in the roots and translocation factor of the metallicolous species compared to the non-metallicolous species was more than 4-folds at the lowest stress level, which decreased with increasing lead concentration. Then, the compatible mechanisms of the metallicolous species have the ability to control the transfer of lead to the shoot in different concentrations, which makes it suitable for growing in lead-contaminated areas.


Alaboudi, K.A., Ahmed, B. and Brodie, G. (2018). Phytoremediation of Pb and Cd contaminated soils by using sunflower (Helianthus annuus) plant. Annals of agricultural sciences, 63(1), 123-127.
Ali, N., Masood, S., Mukhtar, T., Kamran, M.A., Rafique, M., Munis, M. and Chaudhary, H.J. (2015). Differential effects of cadmium and chromium on growth, photosynthetic activity, and metal uptake of Linum usitatissimum in association with Glomus intraradices. Environmental monitoring and assessment, 187, 1-11.
Ashraf, U., Hussain, S., Anjum, S.A., Abbas, F., Tanveer, M., Noor, M.A. and Tang, X.  (2017). Alterations in growth, oxidative damage, and metal uptake of five aromatic rice cultivars under lead toxicity. Plant Physiology and Biochemistry, 115, 461-471.
Bagheri, H., Pakzad, H. and Timori, F. (2011). Investigation on the genesis of metals and ore bearing fluids in Irankuh Pb-Zn deposit. Journal of Stratigraphy and Sedimentology Researches, 27(3), 83-102. (In Farsi)
Baker, A.J. and Brooks, R. (1989). Terrestrial higher plants which hyperaccumulate metallic elements. A review of their distribution, ecology and phytochemistry. Biorecovery, 1, 81-126.
Baker, M. (1987). Metal Tolerance. New Phytologist, 93-111.
Barceló, J. and Poschenrieder, C. (1990). Plant water relations as affected by heavy metal stress: a review. Journal of plant nutrition, 13, 1-37.
Bhatti, S.S., Kumar, V., Sambyal, V., Singh, J. and Nagpal, A.K. (2018). Comparative analysis of tissue compartmentalized heavy metal uptake by common forage crop: a field experiment. Catena, 160, 185-193.
Brunet, J., Varrault, G., Zuily-Fodil, Y. and Repellin, A. (2009). Accumulation of lead in the roots of grass pea (Lathyrus sativus L.) plants triggers systemic variation in gene expression in the shoots. Chemosphere, 77, 1113-1120.
Clemens, S. (2006). Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants. Biochimie, 88, 1707-1719.
Dalyan, E., Yüzbaşıoğlu, E. and Akpınar, I. (2020). Physiological and biochemical changes in plant growth and different plant enzymes in response to lead stress. Lead in plants and the environment, 129-147.
Dellisanti, F., Rossi, P.L. and Valdrè, G.(2009). In-field remediation of tons of heavy metal-rich waste by Joule heating vitrification. International Journal of Mineral Processing, 93(3-4), 239-245.
Dubey, R. and Sharma, P. (2005). Lead toxicity in plants. Brazilian Journal of Plant Physiology, 17, 35-52.
Egendorf, S.P., Groffman, P., Moore, G. and Cheng, Z. (2020). The limits of lead (Pb) phytoextraction and possibilities of phytostabilization in contaminated soil: a critical review. International Journal of Phytoremediation, 22, 916-930.
Fauziah, S.H., Jayanthi, B., Emenike, C.U. and Agamuthu, C. (2017). Remediation of heavy metal contaminated soil using potential microbes isolated from a closed disposal site. International Journal of Bioscience, Biochemistry and Bioinformatics, 7(4), 230-237.
Gadd, G.M. (1994). Interactions of fungi with toxic metals, The Genus Aspergillus. Springer, Boston, pp. 361-374.
Gichner, T., Žnidar, I. and Száková, J. (2008). Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 652, 186-190.
Gopal, R, and Rizvi, A.H. (2008). Excess lead alters growth, metabolism and translocation of certain nutrients in radish. Chemosphere, 70, 1539-1544.
Gupta, D., Nicoloso, F., Schetinger, M., Rossato, L., Pereira, L., Castro, G., Srivastava, S. and Tripathi, R. (2009). Antioxidant defense mechanism in hydroponically grown Zea mays seedlings under moderate lead stress. Journal of Hazardous Materials, 172, 479-484.
Gupta, D., Huang, H. and Corpas, F. (2013). Lead tolerance in plants: strategies for phytoremediation. Environmental science and pollution research, 20, 2150-2161.
Hattab, S., Hattab, S., Flores-Casseres, M.L., Boussetta, H., Doumas, P., Hernandez, L.E. and Banni, M. (2016). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environmental and experimental botany, 123, 1-12.
Jack, E., Hakvoort, H.W., Reumer, A., Verkleij, J.A., Schat, H. and Ernst, W.H. (2007). Real-time PCR analysis of metallothionein-2b expression in metallicolous and non-metallicolous populations of Silene vulgaris (Moench) Garcke. Environmental and experimental botany, 59, 84-91.
Kazakou, E., Dimitrakopoulos, P., Baker, A., Reeves, R. and Troumbis, A. (2008). Hypotheses, mechanisms and trade‐offs of tolerance and adaptation to serpentine soils: from species to ecosystem level. Biological Reviews, 83, 495-508.
Kozhevnikova, A., Seregin, I., Bystrova, E., Belyaeva, A., Kataeva, M. and Ivanov, V. (2009). The effects of lead, nickel, and strontium nitrates on cell division and elongation in maize roots. Russian Journal of Plant Physiology, 56, 242-250.
Kumar, A. and Prasad, M.N.V. (2018). Plant-lead interactions: transport, toxicity, tolerance, and detoxification mechanisms. Ecotoxicology and environmental safety, 166, 401-418.
Li, Y., Zhou, C., Huang, M., Luo, J., Hou, X., Wu, P. and Ma, X. (2016). Lead tolerance mechanism in Conyza canadensis: subcellular distribution, ultrastructure, antioxidative defense system, and phytochelatins. Journal of plant research, 129, 251-262.
Macnair, M.R. (1993). The genetics of metal tolerance in vascular plants. New phytologist, 124, 541-559.
Mahdavian, K., Ghaderian, S.M. and Schat, H. (2016). Pb accumulation, Pb tolerance, antioxidants, thiols, and organic acids in metallicolous and non-metallicolous Peganum harmala L. under Pb exposure. Environmental and experimental botany, 126, 21-31.
Małecka, A., Piechalak, A., Morkunas, I., Tomaszewska, B., 2008. Accumulation of lead in root cells of Pisum sativum. Acta Physiologiae Plantarum 30, 629-637.
Mitra, A., Chatterjee, S., Voronina, A.V., Walther, C. and Gupta, D.K. (2020). Lead toxicity in plants: a review. Lead in plants and the environment, 99-116.
Mohtadi, A., Ghaderian, S.M. and Schat, H. (2012). Lead, zinc and cadmium accumulation from two metalliferous soils with contrasting calcium contents in heavy metal-hyperaccumulating and non-hyperaccumulating metallophytes: a comparative study. Plant and soil, 361, 109-118.
Obiora, S.C., Chukwu, A., Toteu, S.F. and Davies, T.C. (2016). Assessment of heavy metal contamination in soils around lead (Pb)-zinc (Zn) mining areas in Enyigba, southeastern Nigeria. Journal of the geological society of India, 87(4), 453-462.
Park, B. and Son, Y. (2017). Ultrasonic and mechanical soil washing processes for the removal of heavy metals from soils. Ultrasonics sonochemistry, 35,640-645.
Patra, M., Bhowmik, N., Bandopadhyay, B. and Sharma, A. (2004). Comparison of mercury, lead and arsenic with respect to genotoxic effects on plant systems and the development of genetic tolerance. Environmental and experimental botany, 52, 199-223.
Pourrut, B., Shahid, M., Dumat, C., Winterton, P. and Pinelli, E. (2011). Lead uptake, toxicity, and detoxification in plants. Reviews of environmental contamination and toxicology, 213, 113-136.
Pourrut, B., Shahid, M., Douay, F., Dumat, C. and Pinelli, E. (2013). Molecular mechanisms involved in lead uptake, toxicity and detoxification in higher plants. Heavy metal stress in plants, 121-147.
Rascio, N. and Navari-Izzo, F. (2011). Heavy metal hyperaccumulating plants: how and why do they do it? And what makes them so interesting? Plant science, 180, 169-181.
Rucińska-Sobkowiak, R. (2016). Water relations in plants subjected to heavy metal stresses. Acta Physiologiae Plantarum, 38(11), 1-13.
Salehi-Eskandari, B., Ghaderian, S.M. and Schat, H. (2017). The role of nickel (Ni) and drought in serpentine adaptation: contrasting effects of Ni on osmoprotectants and oxidative stress markers in the serpentine endemic, Cleome heratensis, and the related non-serpentinophyte, Cleome foliolosa. Plant and soil, 417, 183-195.
Salehi-Eskandari, B., Ghaderian, S.M.and Schat, H. (2018). Differential interactive effects of the Ca/Mg quotient and PEG-simulated drought in Alyssum inflatum and Fortuynia garcinii. Plant and soil, 428, 213-222.
Salehi-Eskandari, B., Gahrouei, M.S., Boyd, R.S., Rajakaruna, N. and Ghasemi, R. (2022). Physiological responses to lead and PEG-simulated drought stress in metallicolous and non-metallicolous Matthiola (Brassicaceae) species from Iran. South African Journal of Botany, 150,1011-1021.
Shahid, M., Pinelli, E., Pourrut, B. and Dumat, C. (2014). Effect of organic ligands on lead-induced oxidative damage and enhanced antioxidant defense in the leaves of Vicia faba plants. Journal of Geochemical Exploration, 144, 282-289.
Verbruggen, N., Hermans, C. and Schat, H. (2009). Molecular mechanisms of metal hyperaccumulation in plants. New phytologist, 181, 759-776.
Wani, A., Ara, A. and Usmani, J. (2015). Lead toxicity: a review. Interdisciplinary toxicology, 8(2), 55–64.
Weryszko‐Chmielewska, E. and Chwil, M. (2005). Lead‐induced histological and ultrastructural changes in the leaves of soybean (Glycine max (L.) Merr.). Soil Science and Plant Nutrition, 51, 203-212.
Wu, W., Wu, P., Yang, F., Sun, D.L., Zhang, D.X. and Zhou, Y.K.  (2018). Assessment of heavy metal pollution and human health risks in urban soils around an electronics manufacturing facility. Science of the Total Environment, 630, 53-61.
Xu, X., Zhou, Y., Mi, P., Wang, B. and Yuan, F. (2021). Salt-tolerance screening in Limonium sinuatum varieties with different flower colors. Scientific reports, 11, 1-14.
Zhou, C., Huang, M., Li, Y. and Luo, J.  (2016). Changes in subcellular distribution and antioxidant compounds involved in Pb accumulation and detoxification in Neyraudia reynaudiana. Environmental science and pollution research, 23, 21794-21804.
Zulfiqar, U., Farooq, M., Hussain, S., Maqsood, M., Hussain, M., Ishfaq, M., Ahmad, M. and Anjum, M.Z. (2019). Lead toxicity in plants: Impacts and remediation. Journal of environmental management, 250, 109557.