Effect of Application of Salicylic Acid and Biofertilizers on Physiological and Biochemical Characteristics of Chickpea (Cicer arietinum) Cultivars in Rainfed Conditions

Document Type : Research Paper


1 Ph.D. in Crop Physiology, Department of Agriculture and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran

2 Assistant Professor, Department of Agriculture and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran


In order to investigate the effect of salicylic acid and bio fertilizers on the physiological and biochemical characteristics of chickpea cultivars in the dry conditions of Kermanshah province, an experiment was conducted in the form of split factorial design based on randomized complete blocks in three replications in 2018 in the farm of Homil Agricultural Jihad Center in Islam city. Abad-e gharb was implemented. The experimental treatments include three levels (without use, half mill molar and one mill molar) of salicylic acid foliar spraying before flowering and the application of biological fertilizer as a seed in four levels (control, fertile phosphate 2, Petabaror 2 and Petabaror 2 and fertile phosphate 2) and four chickpea varieties. (Adel, Mansour, Azkan and Gokso) and factorially placed in sub-plots. The results of the variance analysis of the data showed that all traits of chlorophyll a, chlorophyll b, total chlorophyll, proline, soluble protein, catalase, superoxide dismutase were affected by salicylic acid and bio fertilizers at the level of 1%, and these traits except the superoxide dismutase trait were also affected by the number. The interaction between salicylic acid and biofertilizers had a significant effect on chlorophyll a, chlorophyll b, total chlorophyll, proline and catalase. The highest percentage of proline and soluble proteins was obtained in the simultaneous combination of salicylic acid and biofertilizers for Adel variety. With the use of 1 millimolar salicylic acid, the amount of proline increased by 21.6% compared to its non-use. So that the highest amount of proline was 0.0884 mg/g fresh weight of the leaf related to the treatment of 1 millimolar salicylic acid. Also, the highest amount of catalase enzyme was in the treatment of not using biofertilizers (0.2937 enzyme units per milligram of protein) and the lowest value was related to the combined use of Fertilizer + Petaferyl phosphate (0.2290 enzyme units per milliliter of protein). So that the amount of catalase enzyme was 22% lower in the treatments using biofertilizers compared to the treatments using fertilizers. Therefore, it can be stated that the simultaneous use of salicylic acid and biofertilizers increase the mentioned physiological activities. What is evident from the results is that the combined use of salicylic acid and biofertilizers had the highest performance compared to the separate use of applied treatments.


Ahmadi, K., Ebadzadeh, H., Hatami, F., Abdshah, H. and Kazemian, A. (2019). Agricultural statistics for 1397-1998, Information and Communication Technology Center of the Ministry of Agricultural Jihad, Volume 1, 15-25. (In Persian).
Abou-Aly, H. E., and M.A. Mady. (2009). Complemented effect of humic acid and biofertilizers on wheat (Triticum aestivum L.) productivity. Annals of Agricultural Sciences. 47(1): 1-12.
Amani, N. Sohrabi, Y. And Heydari, Gh. (2017). Yield and some physiological characteristics of maize using biofertilizers and chemical fertilizers under drought. Journal of Agricultural Knowledge and Sustainable Production. Volume 27. Number 2. (In Persian).
Arnon, D. J. (1956). The chlorophyll absorption spectrum and quantitative determination. Biochemical and Biophysical Acta, 20: 449-461.
Ansari, M.F., Tipre, D.R. and S.R. Dave, a. (2015). Efficiency evaluation of commercial liquid bio-fertilizers for growth of (Cicer aeritinum (chickpea input and field study. Journal of Agriculture Science and Technology. 4(1): 17-24.
Arshadi, M.J., Parsa, M., Lekzian, A. and Kafi, M. (2020). The effect of coexistence of arbuscular mycorrhiza and internal mycorrhiza on grain yield and some physiological characteristics of chickpea (Cicer arietinum) genotypes. Iranian legumes research. Year (Volume) 12 issues, 2 second half. (In Persian).
Babaei, Kh., Seyed Sharifi, R. and Pirzad, A. R. (2019). Effect of biological fertilizers and iron and zinc nano oxides on quantum yield and wheat grain filling process under soil salinity. Agricultural knowledge and sustainable production (agricultural knowledge). Volume, Number 1; From page 73 to page 94. (In Persian).
Baghebani Arani, A., Secondary Teacher, S.A. M., Mashhadi Akbar Bojar, M., Adavi, Z., and Dehghanzadeh Jazi, H. (2019). Effect of Dehydration Stress on Chlorophyll Fluorescence Indices, Photosynthetic Pigments, Trigonline and Fenugreek Seed Yield in Reaction to Zeolite and Nitrogen. Journal of New Findings in Life Sciences. Volume 1, Number 2, Pages 229 to 240.(In Persian).
Bates LS, Waldern RP, and Teave ID. (1973). Rapid determination of free proline for water-stress studies. Plant Soil 39: 205-207
Brito, C., Dinis, L.T., Ferreira, H., Coutinho, J., Moutinho-Pereira, J., Correia, C.M., (2019). Salicylic acid increases drought adaptability of young olive trees by changes in redox status and ionome. Plant Physiol. Biochem. 141, 315–324.
Chance, B. and Maehly, A. C. (1995). Assay of catalase and peroxidase, In: Colowick, S. P., and N. D. Kaplan (eds.), Methods in Enzymology, Academic Press, New York, 2, 764–791.
Chavoshi, S. Normohammadi, GH. Madani, H. Heidari Sharifabad, H. And Alawi Fadhil, A. (2019). Evaluation of the effect of biofertilizers on plant growth stimulants on agronomic traits and physiological characteristics of red bean (Phaseolus vulgaris L) genotypes. Scientific Journal of Crop Physiology, Islamic Azad University, Ahvaz Branch, Volume 11, Number 41, Spring 2017, Pages 79-63. (In Persian).
Dadnia, M. (2018). The relationship between water deficit stress and biofertilizer on the activity of some antioxidant enzymes and their role in changes in barley yield (Hordeum vulgare). Scientific Journal of Plant Ecophysiology. Tenth year, number thirty-three. (In Persian).
Davoodi, A., Zeinalzadeh Tabrizi, H., Shirani Rad, A. (2019). The effect of selenium foliar application on some quantitative and qualitative characteristics of rapeseed cultivars under temperature stress conditions at the end of the season. Eleventh year. No. 32. 87 - 74. (In Persian).
de Vives-Peris, Ollas C., Gomez-Cadenas, A., Perez-Clemente, R.M., (2020). Root exudates: from plant to rhizosphere and beyond. Plant Cell Rep. 39 (1), 3–17.
Demin, I. N., Deryabin, A. N., Sinkevich, M. S. and Trunova, T. I. (2008) Insertion of the cyanobacterial deSa gene coding for Δ12-acyl-lipid deSaturase increases potato plant resistance to oxidative stress induced by hypothermia. Russian Journal of Plant Physiology 55: 710-720.
EL-Tayeb, M.A. (2005). Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation, v.45, p.215-224.
Ervin, E.H., Zhang, X. and Schmidt, R.E. (2005). Exogenous Salicylic acid enhances post-transplant success of heated Kentucky bluegrass and tall fescue sod. Crop Science. 45(1):240–244.
Fukami, J., Paula Cerezini, P., and Hungria, M. (2018). Azospirillum benefits that go far beyond biological nitrogen fixation. AMB Expr (2018) 8:73
Gou, J.Y., Suo, S.Z., Shao, K.Z., Zhao, O., Yao, D., Li, H.P., Zhang, J.L., Rensing, C., (2020). Biofertilizers with beneficial rhizobacteria improved plant growth and yield in chili (Capsicum annuum L.). World J. Microbiol. Biotechnol. 36, 86.
Hadi, M. R. Jafari Nia, M. Balali, Gh. R. (2016). The effect of salicylic acid on the activity of peroxidase, catalase and polyphenol oxidase enzymes in potato plant infected with Rhizoctonia solani. Journal of Cellular and Molecular Research (Iranian Journal of Biology). Volume 29, Number 2. (In Persian)
Heshmati, S. Amini Dehqi, M. And Fathi Amirkhiz, K. (2016). Effect of chemical and biological phosphorus fertilizer on the activity of antioxidant enzymes and some biochemical traits of spring safflower (Carthamus tinctorius L.) under water stress. Journal of production and processing of agricultural and horticultural products. The sixth year. Number 19 (In Persian).
Janda T, Szalai G, Tari I, et al. (2000). hydroponic treatment with Salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants. Planta 1999;208: 175-80.
Jiang W., Zhou H., Bi H., Fromm M., Yang B., Weeks D.P. (2013). Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum, and rice. Nucleic Acids Research, 41 (20): e188 12 páginas.
Keshavarz, H., and Modarres Sanavy, S.A.M. (2014). Effect of salicylic acid on chlorophyll, some growth characteristics, and yield of two canola varieties. Journal Crop Production 7(4): 167-178. (In Persian).
Kumar patel, P., A. Hemantaranjan., AND B.K. Sarma. (2012). Effect of Salicylic acid on growth and metabolism of chickpea under drought stress. Indian J. Plant Physiol., Vol. 17, No. 2, (N.S.) pp. 151-157.
Mittler, R. (2006). Abiotic stress, the field environment, and stress combination. Trends in Plant Science. 11: 15–19.
Momeni, F., Abdali Mashhadi, A., Siadat, S. A., Pakdaman Sardrood, B., Mokhtar Ghobadi, M. (2020). Effect of application of biofertilizers and salicylic acid on biochemical characteristics and grain elements of chickpea cultivars (Cicer arietinum L.) under dryland conditions of Kermanshah. Scientific Journal of Crop Physiology, Islamic Azad University, Ahvaz Branch, year, 12 issues, 47. Pages 25- 5. (In Persian)
Movahedi Dehnavi, M., Niknam, M., Behzadi, Y., Mohtashami, R., and Bagheri, R. (2017). Comparison of physiological responses of flax (Linum usitatissimum L.) to drought and salinity stress and foliar application with salicylic acid. Iranian Journal of Plant Biology, Year 9, Issue 33. Pages 62 - 39. (In Persian)
Noorzad, Sh., Ahmadian, A. And Moghaddam, m. (2015). Evaluation of proline content, chlorophyll index, carbohydrate and nutrient uptake in coriander under drought stress and fertilizer treatment. Iranian Journal of Crop Research. 13 (1): 139-131. (In Persian)
Padash, A., Ghanbari, A. And Asgharipour, M. R. (2016). The effect of salicylic acid on the concentration of some end elements, proteins, and antioxidant enzymes of basil under lead stress. Plant Biology of Iran. 8 (27:) 32-17. (In Persian).
Pakar, N., Pirasteh -Anosheh, H. and Emam, Y. (2016). Barley growth, yield, antioxidant enzymes, and ion accumulation as affected by PGRs under salinity stress conditions. Journal of Plant Nutrition. 39(10): 1372 -1379.
Pezeshkpour, P., ardakani, M.R. Pakenjad, F. vazan, S. (2014). Effect of application of vermicompost, mycorrhizal symbiosis, and biphosphate solvent on physiological traits and yield of chickpea. Journal of Crop Physiology. Volume 6, number 53. (in Persian).
Pirasteh-Anosheh, H., Ranjbar, G., Pakniyat, H. and Emam, Y. (2016). Physiological Mechanisms of Salt Stress Tolerance in Plants; an Overview. p. 141-160. In: Azooz, M. M. and P. Ahmad (Eds.). Plant Environment Interaction: Responses and Approaches to Mitigate Stress. John Wiley & Sons, London.
Porabethaj, M. Habibi, d. Pakenjad, F. Davoodifar, M. And Farahani Pod, p. (2012). The effect of growth-promoting bacteria and foliar application of silicic acid and amino acids on the activity of biochemical biomarkers in drought stress conditions in barley (Hordeum vulgar L.). Journal of Agriculture and Plant Breeding. Volume 8, Number 3, pp. 138 - 127. (In Persian)
Qalavand, A., Mohammadi, Kh., Agha Alikhani, M., Sohrabi, Y. (2012). The effect of different organic and biological fertilizers on yield and yield components of chickpea (Cicer arietinum L.) Journal of Agriculture. Volume 94: 49 - 41. (in Persian).
Ram Rao, D.M., Kodandaramaiha, J., Reddy, M.P., Katiyar R.S. and Rahmathulla, V.K. (2007). Effect of VAM fungi and bacterial biofertilizers on mulberry leaf quality and silk worn cocoon characters under semi-arid conditions. Caspian Journal of Environmental Science. 5(2): 111-117.
Rezaei Alulu, A. Khairy, A. Sany khani, M. Arghavany, M. (2020). The effect of foliar application of salicylic acid, glycine betaine, and gamma-aminobutyric acid on the antioxidant activity of Carla under dehydration stress. Scientific Journal of Plant Ecophysiology, Twelfth Year, No. 40. (In Persian).
Roussos, P. A., Gasparatos, D., Kechrologou, K., Katsenos, P. and Bouchagier, P. (2017). Impact of organic fertilization on soil properties, plant physiology, and yield in two newly planted olive (Olea europaea L.) cultivars under Mediterranean conditions. Scientia Horticulturae, 220: 11- 19.
Rudresh DL., MK. Shivaprakash., and RD. PraSad, (2005). Effect of combined application of Rhizobium, phosphate solubilizing bacterium, and Trichoderma spp. on growth, nutrient uptake, and yield of chickpea (Cicer aritenium L.). Applied Soil Ecology 28: 139-146.
Sabaghpour, S.H. (2015). Strategic Framework for Food Legume Research. Chap & Entesharat
Organization, 412 pp (In Persian).
Sandhya, V., S. K. Z. Ali, M. Grover, G. Reddy and B. Venkateswarlu. (2010). Effect of plant growth-promoting Pseudomonas spp. on compatible solutes, antioxidant status, and plant growth of maize under drought stress. Plant Growth Regulation 62:21-30.
Saydi, Z., Abbasi, N.A. and Zare, M.J. (2021). The effect of nitroxin biofertilizer on physiological characteristics of four black seed ecotypes (Nigella sativa L.) under drought stress. Journal of Soil-Plant Relations. Year 12. Issue 2 Pages 32 - 19. (In Persian).
Seadatmand, M. And Publishing, Sh. (2012). Effect of silicon pretreatment time on salinity tolerance in Iranian borage. Greenhouse science and technology. third year. Number twelve. 56 - 45. (In Persian).
Shinde, M. Khade, Sh. (2019). Effect of Biofertilizers on Chlorophyll contents of Maize (Zea mays L.) Variety Eco-92. International Journal of Life Sciences Research. Vol. 7, Issue 2, pp: (304-307), Month: April - June 2019,
Shuriabi, M., Ganjali, A. Abrisham chi, p. (2012). The effect of salicylic acid on the activity of enzymes and antioxidant compounds of chickpea cultivars (Cicer arietinum L) in the face of drought stress. Journal of Environmental Stresses in Crop Sciences. 5 (1): 54 - 51. (In Persian).
Yazdanpanah, S., Baghizadeh, A., Abbassi, F. (2011). The interaction between drought stress and salicylic and ascorbic acids on some biochemical characteristics of Satureja hortensis. Afr. J. Agron. 6 798–807