Investigation of Biochar Application and Different Levels of Irrigation on Physico-chemical Properties and Microbial Respiration of Cadmium Contaminated Soil in Tomato Cultivation

Document Type : Research Paper


1 Faculty of Agriculture, Soil Science Department,, Lorestan University, Khoramabad, Iran

2 Assistant Professor, Soil Science Department, Faculty of Agriculture Lorestan University, Khoramabad, Iran

3 Assistant Professor, Department of Water Engineering, Faculty of Agriculture, Lorestan University, Iran

4 Associate Professor, Soil Science Department, Faculty of Agriculture, Lorestan University, Iran

5 Assistant Professor, Agronomy and Plant Breeding Department, Faculty of Agriculture, Lorestan University, Iran


Soil pollution with heavy metals and moisture stress are the main threats to food security in the world. The aim of this study was to investigate the effect of biochar on physico-chemical properties and soil microbial respiration in reducing cadmium (Cd) stress in tomatoes, and to determine the optimal irrigation level for plant growth. A factorial-based experiment in a randomized complete block design using three levels of rice bran (0 (B0), 3 (B3), and 6 (B6) ton/ha) and seven irrigation levels (50 (L50), 60 (L60), 70 (L70), 80 (L80), 90 (L90), 100 (L100), and 110 (L110) percent of full irrigation (L100) based on soil moisture depletion, respectively  were performed on tomato cultivation in the research greenhouse, Faculty of Agriculture, Lorestan University, in 2019. The results showed that the best treatments in improving soil physical properties (bulk density, total porosity, mean weight diameter, water-stable aggregates, and stability index), soil nutrients (nitrogen, phosphorus, potassium, iron, manganese, zinc, and copper), and increasing soil microbial respiration, were L90B6, L100B6, and L110B6 treatments. The best tomato fruit yield with 1459.00, 1588.70, and 1610.30 g plant-1 was observed in L90B6, L100B6, and L110B6 treatments, respectively, which have not significantly different. In addition, Cd concentration in tomato fruit in these treatments was 0.02 mg/kg, which is lower than that of the global average (FAO/WHO), while in treatments B0 and B3, severe toxicity of tomato fruit and reduced yield were observed. Therefore, in the irrigation level of %90 percent of soil moisture depletion (L100) with the application of 6 ton/ha rice husk biochar (B6), minimum Cd stress and maximum yield for the plant were observed, and more water consumption was prevented.


Abdel-Shafey, H., Hegemann, W., & Teiner, A. (1994). Digestion with concentrated HNO3 and H2O2. Environment Management and Health, 5, 21-24.
Albert, H. A., Li, X., Jeyakumar, P., Wei, L., Huang, L., Huang, Q., Kamran, M., Shaheen, S. M., Hou, D., & Rinklebe, J. (2021). Influence of biochar and soil properties on soil and plant tissue concentrations of Cd and Pb: A meta-analysis. Science of the Total Environment, 755, 142582.
Almaroai, Y. A., & Eissa, M. A. (2020). Effect of biochar on yield and quality of tomato grown on a metal-contaminated soil. Scientia Horticulturae, 265, 109210.
Anderson, J. P. E. (1982). Soil respiration. In: A.L. and R. H. Mille (Ed.), Methods of Soil Analysis. Part 2, Chemical and Microbiological Properties. American Society of Agronomy. Madison, WI.
Bai, N., Zhang, H., Li, S., Zheng, X., Zhang, J., Zhang, H., Zhou, S., Sun, H., & Lv, W. (2019). Long-term effects of straw and straw-derived biochar on soil aggregation and fungal community in a rice–wheat rotation system. PeerJ, 6, e6171.
Bagheri, M.,  Javanmanrd, H. R., & Naderi, M. R. (2021). The growth of (Matricaria chamomilla L.) affected by cadmium and lead in greenhouse and field conditions. Bi-Quarterly Journal of Plant Production, 11 (1): 19-34.
Blake, G. R., & Hartge, K. (1986). Bulk density. In A. Klute (Ed.), Methods of soil analysis: Part 1 Physical and mineralogical methods (Vol. 5, pp. 363-375).
Biria, M., Moezzi, A., AmeriKhah, H. (2017). 'Effect of Sugercan bagasse,s biochar on maize plant growth, grown in lead and cadmium contaminated soil,s', Water and Soil, 31(2), pp. 609-626. doi: 10.22067/jsw.v31i2.55832.
Brennan R.F., Armour J.D., and Reuter D.J. (1993). Diagnosis of zinc deficiency. In A.D. Robson (ed.) Zinc in Soils and Plants, P206. Springer, Netherlands. p. 167-181.
Cantrell, K. B., Hunt, P. G., Uchimiya, M., Novak, J. M., & Ro, K. S. (2012). Impact of pyrolysis temperature and manure source on physicochemical characteristics of biochar. Bioresource technology, 107, 419-428.
Carter, M. R., & Gregorich, E. G. (2007). Soil sampling and methods of analysis. Boca Raton, Florida: CRC press.
Chen, L., Long, C., Wang, D., & Yang, J. (2020). Phytoremediation of cadmium (Cd) and uranium (U) contaminated soils by Brassica juncea L. enhanced with exogenous application of plant growth regulators. Chemosphere, 242, 125112.
Chen, Z., Lu, Z., Zhang, Y., Li, B., Chen, C., & Shen, K. (2021). Effects of biochars combined with ferrous sulfate and pig manure on the bioavailability of Cd and potential phytotoxicity for wheat in an alkaline contaminated soil. Science of the Total Environment, 753, 141832.
Dad, K., Nawaz, M., Hassan, R., Javed, K., Shaheen, A., Zhao, F., Imran, M., Shah, S., Anwar, M., & Aurangzaib, M. (2021). Impact of biochar on the growth and physiology of tomato grown in the cadmium contaminated soil. Pakistan Journal of Agricultural Research, 34(2), 454-462.
Dhaliwal, S. S., Singh, J., Taneja, P. K., & Mandal, A. (2020). Remediation techniques for removal of heavy metals from the soil contaminated through different sources: a review. Environmental Science and Pollution Research, 27(2), 1319-1333.
Domingues, R. R., Trugilho, P. F., Silva, C. A., Melo, I. C. N. d., Melo, L. C., Magriotis, Z. M., & Sanchez-Monedero, M. A. (2017). Properties of biochar derived from wood and high-nutrient biomasses with the aim of agronomic and environmental benefits. PloS one, 12(5), e0176884.
El Namas, A. (2020). Ameliorating effect of biochar on some physical properties of sandy soil and water use efficiency of tomato (Solanum lycopersicum L.) plant grown under drip irrigation. Journal of Soil Sciences and Agricultural Engineering, 11(7), 231-240.
Fu, B., Chen, L., Huang, H., Qu, P., & Wei, Z. (2021). Impacts of crop residues on soil health: A review. Environmental Pollutants and Bioavailability, 33(1), 164-173.
Gee, G.W., and Bauder, J.W. (1986). Particle size analysis. P 383-411, In: A. Klute (ed.), Methods of soil analysis, 2nd ed. Agron. Monogr. 9. ASA. Madision, WI.
Gharahi, N. (2022).Effect of Biochar and Zeolite on Cadmium Uptake in Green bell Pepper (Capsicum Annuum) and Leaching in Saline-alkaline Soil. Journal of water and soil resources conservation, 11(2), 69-78.
Ghorbani, M., Asadi, H., & Abrishamkesh, S. (2019). Effects of rice husk biochar on selected soil properties and nitrate leaching in loamy sand and clay soil. International soil and water conservation research, 7(3), 258-265.
Hale, L., Curtis, D., Azeem, M., Montgomery, J., Crowley, D. E., & McGiffen Jr, M. E. (2021). Influence of compost and biochar on soil biological properties under turfgrass supplied deficit irrigation. Applied Soil Ecology, 168, 104134.
Hillel, D. (1980). Fundamentals of soil physics. New York: Academic press.
Hossain, M., Bahar, M., Sarkar, B., Donne, S., Ok, Y., Palansooriya, K., Kirkham, M., Chowdhury, S., & Bolan, N. (2020). Biochar and its importance on nutrient dynamics in soil and plant. Biochar, 2, 379-420.
Huang, H., Reddy, N. G., Huang, X., Chen, P., Wang, P., Zhang, Y., Huang, Y., Lin, P., & Garg, A. (2021). Effects of pyrolysis temperature, feedstock type and compaction on water retention of biochar amended soil. Scientific Reports, 11(1), 1-19.
Ibrahim, A., Marie, H., & Elfaki, J. (2021). Impact of biochar and compost on aggregate stability in loamy sand soil. Agricultural Research Journal, 58, 34-44.
Karimi, A., Moezzi, A., Chorom, M., & Enayatizamir, N. (2020). Application of biochar changed the status of nutrients and biological activity in a calcareous soil. Journal of Soil Science and Plant Nutrition, 20(2), 450-459.
Khosropour, E., Weisany, W., Tahir, N. A. R., & Hakimi, L. (2021). Vermicompost and biochar can alleviate cadmium stress through minimizing its uptake and optimizing biochemical properties in Berberis integerrima bunge. Environmental Science and Pollution Research, 1-11.
Liang, J., Li, Y., Si, B., Wang, Y., Chen, X., Wang, X., Chen, H., Wang, H., Zhang, F., & Bai, Y. (2021). Optimizing biochar application to improve soil physical and hydraulic properties in saline-alkali soils. Science of the Total Environment, 771, 144802.
Lindsay, W. L., & Norvell, W. (1978). Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil science society of America journal, 42(3), 421-428.
Liu, J., Huang, W., Li, Z., Hu, J., Zhu, Y., Xie, H., & Peng, C. (2020). Accumulation, subcellular distribution, and ecological risk assessment of Pb and Cd in Bellamya aeruginosa from the Xiangjiang River, China. Chemistry and Ecology, 36(4), 327-341.
Liu, Q., Liu, B., Zhang, Y., Hu, T., Lin, Z., Liu, G., Wang, X., Ma, J., Wang, H., & Jin, H. (2019). Biochar application as a tool to decrease soil nitrogen losses (NH3 volatilization, N2O emissions, and N leaching) from croplands: Options and mitigation strength in a global perspective. Global change biology, 25(6), 2077-2093.
Majeed, A., Niaz, A., Rizwan, M., Imran, M., Alsahli, A. A., Alyemeni, M. N., & Ali, S. (2021). Effects of biochar, farm manure, and pressmud on mineral nutrients and cadmium availability to wheat (Triticum aestivum L.) in Cd‐contaminated soil. Physiologia Plantarum, 173(1), 191-200.
Mandal, S., Pu, S., Adhikari, S., Ma, H., Kim, D.-H., Bai, Y., & Hou, D. (2021). Progress and future prospects in biochar composites: Application and reflection in the soil environment. Critical Reviews in Environmental Science and Technology, 51(3), 219-271.
Mehrab, N., & Chorom, M. (2014). Leaching of nitrogen in the presence of zeolite enriched with ammonium in two soil textures under wheat cultivation. Water and Soil Science, 24(2), 159-170. (In Farsi)
Mehrab, N., Chorom, M., Norouzi Masir, M., Fernandes de Souza, M., & Meers, E. (2021). Alteration in chemical form and subcellular distribution of cadmium in maize (Zea mays L.) after NTA-assisted remediation of a spiked calcareous soil. Arabian Journal of Geosciences, 14(21), 1-14.
Melomey, L. D., Danquah, A., Offei, S. K., Ofori, K., Danquah, E., & Osei, M. (2019). In S. T. Nyaku & A. Danquah (Ed.), Review on tomato (Solanum lycopersicum L.) improvement programmes in Ghana: Recent advances in tomato breeding and production (Vol. 49, pp. 49-69).
Meng, Q., Zhao, S., Geng, R., Zhao, Y., Wang, Y., Yu, F., Zhang, J., & Ma, X. (2021). Does biochar application enhance soil salinization risk in black soil of northeast China (a laboratory incubation experiment)? Archives of Agronomy and Soil Science, 67(11), 1566-1577.
Moradi, N., & Karimi, A. (2021). Fe-modified common reed biochar reduced cadmium (Cd) mobility and enhanced microbial activity in a contaminated calcareous soil. Journal of Soil Science and Plant Nutrition, 21(1), 329-340.
Nelson, D.W. and Sommers, L.E. (1996). Total carbon, organic carbon, and organic matter. In: Sparks, D.L. (Ed.), Methods of Soil Analysis, Part 3, Chemical Methods, SSSA and ASA, Madison, WI. PP. 961-1010.
Olsen S.R., Cole C.V., Watanabe F.S. and Dean L.A. (1954). Estimation of available phosphorus in soils by extraction with sodium bicarbonate. U.S. Dept. of Agric. Circ. 939p.
pourmansour, S., Razzaghi, F., Sepaskhah, A., Moosavi, A. (2019). 'Wheat growth and yield investigation under different levels of biochar and deficit irrigation under greenhouse conditions', Water and Irrigation Management, 9(1), pp. 15-28. doi: 10.22059/jwim.2019.278053.665
Pieri, C. J. (1992). Fertility of soils: A future for farming in the West African savannah (1 st ed). Berlin: Springer Verlag.
Ren, T., Li, J., Feng, H., Yun, F., Chen, N., Wang, H., Yin, Q., Liu, H., Yek, P. N. Y., & Lam, S. S. (2021). Micro-particle biochar for soil carbon pool management: Application and mechanism. Journal of Analytical and Applied Pyrolysis, 157, 105229.
Safian, M., Motaghian, H., & Hosseinpur, A. (2020). Effects of sugarcane residue biochar and P fertilizer on P availability and its fractions in a calcareous clay loam soil. Biochar, 2(3), 357-367.
Salam, A., Bashir, S., Khan, I., Hussain, Q., Gao, R., & Hu, H. (2019). Biochar induced Pb and Cu immobilization, phytoavailability attenuation in Chinese cabbage, and improved biochemical properties in naturally co-contaminated soil. Journal of Soils and Sediments, 19(5), 2381-2392.
Sanjari, S., Boroomand, N., and Moghbeli, M. (2021). The Concentration of Lead and Cadmium in Some Greenhouse Products and its Effect on Human Health. Journal of Environmental Science and Technology, 23(8), 95-106.
Singh, B., Camps-Arbestain, M., & Lehmann, J. (2017). Biochar: a guide to analytical methods. Boca Raton, Florida: CRC Press.
Sumner M.E., and Miller W.P. (1996). Cation exchange capacity, and exchange coefficients. In D.L. Sparks (ed.), Methods of soil analysis. p1320. Part 2: Chemical properties, (3rd ed.) ASA, SSSA, CSSA, Madison, WI. p. 1201-1231.
Thomas, G. W. (1996). Soil pH and soil Acidity. In: sparks, D. L. (Ed). Methods of soil analysis. Part 3- Chemical Methods. Soil Sci. Soc. Am. Inc. Book series, Madison, WI. No. 5. pp: 475-490.
Tian, X., Li, Z., Wang, L., Wang, Y., Li, B., Duan, M., & Liu, B. (2020). Effects of biochar combined with nitrogen fertilizer reduction on rapeseed yield and soil aggregate stability in upland of purple soils. International Journal of Environmental Research and Public Health, 17(1), 279.
Walkley, A., & Black, I. A. (1934). An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil science, 37(1), 29-38.
Wang, Y., Zheng, K., Zhan, W., Huang, L., Liu, Y., Li, T., Yang, Z., Liao, Q., Chen, R., & Zhang, C. (2021). Highly effective stabilization of Cd and Cu in two different soils and improvement of soil properties by multiple-modified biochar. Ecotoxicology and Environmental Safety, 207, 111294.
Xu, C., Zhao, J., Yang, W., He, L., Wei, W., Tan, X., Wang, J., Lin, A. (2020). Evaluation of biochar pyrolyzed from kitchen waste, corn straw, and peanut hulls on immobilization of Pb and Cd in contaminated soil. Environmental Pollution, 261, 114133.
Xu, X., Cao, X., & Zhao, L. (2013). Comparison of rice husk-and dairy manure-derived biochars for simultaneously removing heavy metals from aqueous solutions: role of mineral components in biochars. Chemosphere, 92(8), 955-961.
Zeeshan, M., Ahmad, W., Hussain, F., Ahamd, W., Numan, M., Shah, M., & Ahmad, I. (2020). Phytostabalization of the heavy metals in the soil with biochar applications, the impact on chlorophyll, carotene, soil fertility and tomato crop yield. Journal of Cleaner Production, 255, 120318.
Zhang, C., Huang, X., Zhang, X., Wan, L., & Wang, Z. (2021). Effects of biochar application on soil nitrogen and phosphorous leaching loss and oil peony growth. Agricultural Water Management, 255, 107022.
Zhang, C., Li, X., Yan, H., Ullah, I., Zuo, Z., Li, L., & Yu, J. (2020b). Effects of irrigation quantity and biochar on soil physical properties, growth characteristics, yield and quality of greenhouse tomato. Agricultural Water Management, 241, 106263.
Zhang, Q., Song, Y., Wu, Z., Yan, X., Gunina, A., Kuzyakov, Y., & Xiong, Z. (2020a). Effects of six-year biochar amendment on soil aggregation, crop growth, and nitrogen and phosphorus use efficiencies in a rice-wheat rotation. Journal of Cleaner Production, 242, 118435.
Zwolak, A., Sarzyńska, M., Szpyrka, E., & Stawarczyk, K. (2019). Sources of soil pollution by heavy metals and their accumulation in vegetables: A review. Water, air, & soil pollution, 230(7), 1-9