Investigating the Effect of Coated Nanosilicon Oxide with Humic Acid on Yield, Ion Composition and Salinity Tolerance of Black Cumin(Nigella sativa L.)

Document Type : Research Paper


1 Research Instructor Greenhouse Group, Soil and Water Research Department, Isfahan Agricultural and Natural Resources Research and Education Center Agricultural Research Education and Extension Organization (AREEO), Isfahan, Iran,

2 Soil Science Department, Faculty of Agriculture, Lorestan University ,Lorestan, Iran

3 Assistant Professor, Department of Chemistry, Faculty of Applied Sciences, Lorestan University, Lorestan, Iran

4 Assistant Professor of Medicinal Plants group, Natural Resource Research Department, Isfahan Agricultural and Natural Resources Research and Education Center, Agricultural Research,Education and Extension Organization (AREEO), Isfahan, Iran


This study was conducted to investigate the effects of coated nanosilicon oxide with humic acid (coHA-nSi) on yield, ion composition and salt tolerance of black cumin (Nigella sativa L.) in 2017-2018. The experiment was performed in the form of split-plot based on Randomized Complete Block Design with four replicates under greenhouse condition. The main plot was salinity levels including 2 (control), 3.5 and 5 dS/m. The sub plots was SiO2-nanoparticles including Control (without nanosilicon oxide), uncoated nanosilicon oxide (0.5 g/l) and coated nanosilicon oxide (0.5 g/l) which were applied in multi leave stage (15 days after planting) and flowering stages (50 days after planting). The results demonstrated that the salinity stress decreased yield and its compouents significantly. The application of coated nano silicon oxide increased significantly the seed weight (18.9%) and biomass dry weight (8.9%), dry root weight (23.7), number of capsules (24%) and number of seed (15.9%), harvest index (10.5%) and relative leaf moisture content (20.7%) and decreased significantly the ionic leakage (IL, 18.1%) compared to the uncoated nanoparticles. Also, it increased the uptake of leaf nutrients such as nitrogen, potassium and calcium. Therefore, it is concluded that the silicon nanooxide coated with humic acid could increase the effect of this nanoparticle on salinity tolerance indices, quantitative and qualitative traits of black seed under saline conditions.


Main Subjects

Abdelazim, Y., Abdallah, E., Gaballah, M and Zaghloul, S. (2017). Role of Silicon Dioxide Nano Fertilizer in Mitigating Salt Stress on Growth, Yield and Chemical Composition of Cucumber (Cucumis sativus L.). Inte. J. Agric. Res.12(3): 130-135.
Aghaeifard, F., Babalar, M., Fallahi, E. and Ahmadi, A. (2015). Influence Of humicacid and salicylic acid on yield, fruit quality and leaf mineral elements of strawberry (FragariaananassaDuch.) cv.camarosa. Journal of Plant Nutrition,39(13): 1821-1829.
Agarie, S., Uchida, H., Agata, W., Kubota, F. and Kaufman, P.B. (1993). “Effect of silicon on growth, dry matter production and photosynthesis in rice plant (Oryza stiva). “Crop Prod. Improve, Technol, 34: 225-234.
Ashraf, M., Mukhtar, N., Rehman,S. and Rha, E. S. (2004). Salt induced changes in photosynthetic activity andgrowth in a potential medicinal plant Bishop`s weed (Ammi majus L.). Photosynthetica, 42(4): 543-550.
Aydin A and Metin T, 2012. Humic acid application alleviates salinity stress of bean (Phaseolus vulgaris L.) plants decreasing membrane leakage. African Journal of Agricultural Research, 7(7): 1073-1086.
Baghalian, K. Haghiry, A. Naghavi, M.R. and Mohammadi, A. (2008). Effect of saline irrigation on agronomical and phytochemical characters of chamomile (Matricaria recutita L.). Scientia Horticulturae, 116. 437-441.
Castiglione Monica, R. and Cremonini, R. (2009). Nanoparticles and higher plants. Caryologia, 62: 161-165, 2009.
 Chamani, A., Bonyadi, M. And Ghanbari, A. (2015). Effect of salicylic acid and humic acid on growth indicators of ornamental medicinal plant Parvanesh (Catharanthus roseus L.). Journal of Horticultural Sciences (Agricultural Sciences and Industries), Volume 29, Number 4: 641-631 (In Farsi).
Cimrin, K. M., Türkmen, O., Turan, M. and Tuncer, B. 2010. Phosphorus and humic acid application alleviate salin- ity stress of pepper seedlings. African Journal of Biotechnology, 9, 5845–5851.
Daneshmand, F., Arvin, M. J. and Keramat, B. 2014. Salicylic acid induced changes in safflower (Carthamus tinctorius L.) under salinity stress. Iranian Journal of Plant Biology, 27 (2): 204-215 (in Farsi).
Daur, I. and Bakhashwain, A. A. 2013. Effect of humic acid on growth and quality of maize fodder production. Pakistan Journal of Botany, 45:21-25.
Derosa, M. R., Monreal, C., Schmitzer, M., Walsh, R. and Sultan, Y. 2010. Nanotechnology in fertilizers. National Nanotechnology 1: 193-225.
Dkhil BB and Denden M. 2012. Effect of salt stress on growth, anthocyanins, membrane permeability and chlorophyll fluorescence of Okra (Abelmoschus esculentus L.) seedlings. Amer. J. Plant Physiol, 7: 174 - 83.
Epstein, E. 1999. Silicon. Plant Physiol. 50: 641-664.
Emami, A. 1996. Metod of leaf analies. Journal of Soil and Water Institiue, 982(2): 65-73(in Farsi).
Forgac, L. and Czimbalmos, R. 2011. The applied soilprotective cultivation system-a method to reduce and prevent the soil degradation processes. Novenytermeles, 60: 279-282.
Fazeli, A., Zarei, B. and Tahmasebi, Z. 2018. The effect of salinity stress and salicylic acid on some physiological and biochemical traits of Black cumin (Nigella sativa L.). Iranian Journal of Plant Biology, 9(4):69-83(In Farsi).
Gad El-Hak, S. H., Ahmed, A. M. and Moustafa, Y. M. M. 2012. Effect of Foliar Application with Two Antioxidants and Humic Acid on Growth, Yield and Yield Components of Peas (Pisum sativum L.). Journal of Horticultural Science and Ornamental Plants, 4 (3): 318-328.
Gholami, H., Samawat, S. And paper Ardabili, Z. 2012. Effects of humic acid and folic acid on the resistance of saffron to salinity stress. Master Thesis, Islamic Azad University, Garmsar Unit (In Farsi).
Ghorbani, S., H. R. Khazaei., M. Kafi and Banayan aval, M. 2010. Effect of application of humic acid on irrigation water on yield and yield components of corn (Zea mays L.) yield. Journal of Agricultural Ecology, 2(1):111-118(In Farsi).
Gohari, G., Rasouli, F. and Zahedi, S. M. 2017.”Evaluation of some growth traits and quantitativecharacteristics of basil (Ocimum basilicum L.)In conditions of salt stress and application of humic acid”, Journal of Agricultural Knowledge and Sustainable Production, 27(2): 159-168.
Heckman, J. 2013. Silicon: a beneficial substance. Better crops, 97(4): 14-16.
Heidari Sharifabad, H. 2001. Plant & Salinity. Institute of Forests and Rangelands Research, P 199 (in Farsi).
Haghighi, M. and Pessarakli, M. 2013. Influence of silicon and nano-silicon on salinity tolerance of cherrytomatoes (Solanum lycopersicum L.) at early growth stage. Scientia Horticulturae, 161: 111–117.
Hajar, A. S., Zidan, M. A. and Al-zahrani, H. S. 1996. Effect of salinity stress on the germination, growth and physiological activities of Black cumin (Nigella sativa L.)”, The Arab Gulf Journal of Science Reseach, 14(2): 445-454.
Hoagland, D. R. and Arnon, D. I. 1950. The waterculturefor growing plants without soil. CaliforniaAgriculture Experimental Statistics Circular.
Jaleel, C. A., Sankar, B., Sridharan, R. and Panneerselvam, R. (2013) Soil salinity alters growth, chlorophyll content and
secondary metabolite accumulation in Catharanthus roseus. Turkish Journal of Biology 32: 79-83.
Jarošová M, Klejdus B, Kovacik J, Babula P and Hedbavny J. 2016. Humic acid protects barley against salinity. Acta Physiologiae Plantarum, 38(6): 1-9.
Kalteh, M., Alipour, Z. T., Ashraf, S., Marashi-A, M. and Falah-N, A. 2014. Effect of silica Nanoparticles on Basil (Ocimum basilicum) Under Salinity Stress. Journal of Chemical Health Risks, 4(3): 49 –55.
Karakurt, Y., Huvnlu, H., Unla, H. and Padem, P. 2009. The influence of foliar and soil fertilization of humic acid on yield and quality of pepper. Acta Agric. Scand, 59: 233-237.
Kaya C., Kirnak H., Higgs D. and Saltali K. 2002. Supplementary calcium enhances plant growth at fruit yield instrawberry cultivars grown at high (NaCl) salinity. J. Scientia Horticulturae, 93:65-74.
Kaya, M. D., Okci, G., Atak, M., Cikili, Y. and Kolsarici, O. 2006. Seed treatment to overcome salt anddrought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24: 291- 295.
Khalid, A. 2015. Seed yield, fixed oil, fatty acids and nutrient content of nigella sativa L.cultivated under salt stress conditions. Journal of Agronomy, 14 (4): 241-246.
Khattak, R. A., Haroon, K. and Muhammad, D. 2013. Mechanism(s) of humic acid induced beneficial effects in saltaffected soils. Scientific Research and Essays - AcademicJournal, 8: 932-939.
Khorasaninejad, S., Mousavi, A., Soltanloo, H., Hemmati, Kh. and Khalighim, A. 2010.”The Effect of Salinity Stress on Growth Parameters, Essential oil Yield and Constituent of eppermint (Mentha piperita L)”,World Applied Sciences Journal, 11(11): 1403-1407.
Khoshgoftarmanesh.H, 2010. Advanced topics in plant nutrition. Isfahan University of Technology Publishing Center, 369 p. (In Farsi).
Koocheki, A., Fallahi, H.R., Amiri, M.B. and Ehyaei,H.R. 2016. Effects of humic acid application and
mother corm weight on yield and growth of Saffron.Journal of Agroecology, 7(4): 425-442.
Liang, L., Luo, L. and Zhang, S.2011. Adsorption and desorption of humic and fulvic acids on SiO2 particles at nano and micro-scales. Colloids and Surfaces A: Physicochem English Aspects, 384:126– 130.
 Lutts, S., J. M. Kient and J. Bauharmont. 1996. NaCl induced senescence in leaves of rice (Oryza sativa) cultivars differing in salinity resistance. Annals of Botany, 78: 389-398.
Ma, J.F. and Takahashi, E. 1993. Interaction between calcium and silicon in water-culyured rice plants. Plant soil, 148: 107-113.
Mahmoudi, M., Samavat, S., Mostafavi, M., Khalighi, A.and Cherati, A. 2013. The effects of proline and humic acid on quantitative properties of kiwi fruit. International ResearchJournal Appliedand Basic Science, 6 (8): 1117-1119.
Marschner, H. 1995. Mineral nutrition of higher plant, Academic Liang press, London.
Mishra, A. and Choudhuri, M. A.1999. Effects ofsalicylic acid on heavy metal induced membranedeterioration mediated by lipoxygenase in rice.Biologia Plantarum, 42: 409-415.
Mohaghegh, P., Shirvani, M., and Ghasemi, S. 2010. Silicon application effects on yield and growth of two cucumber genotypes in hydroponics system. Journal of Science and Technology of Greenhouse Culture-Isfahan University of Technology, 1(1), 35-40.
Momeni, N., Arvin, M., Khagoei negad, Gh. Keramat, B. and Daneshmand, F.2013. Effects of sodium chloride and
salicylic acid on some photosynthetic parameters and mineral nutrition in maize (Zea mays L.) plants. PlantBiology 5(15): 15-30 (in Farsi).
Narimani, R; Moghadam, M; Nemati, H; Ghasemi Pir Bolouti, AS, 2018,  Evaluation of salinity stress modification using humic acid and ascorbic acid in Badrshabi medicinal plant ", Iranian Journal of Biology, Volume 31, Number 2, (In Farsi).
Naseri, M., Arvii, H., Nemati, S. H. And Kafi, M. 2011. The effect of different levels of salinity and silicon on biomass production, amount of sodium and potassium in the aerial parts of fenugreek. Journal of Water and Soil, Volume 26, Number 2, 514-508 (In Farsi).
Nikbakht, AS; Kafi, M., Bablar, M., Etemadi, N. Ebrahimzadeh, H. Piang Shia, M. 2007. Effect of iomimetic Acid on Calcium and Physiological Behavior after Harvesting Gerbera Flowers. Iranian Journal of Horticultural Science and Technology, 8(4), 248-237.
Parida, A. K. and Das, A. B. 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60, 324-349.
Pirasteh Anousheh, H., Rousta, M. J. And Imam Y. 2015. Different methods of treating crops with salicylic acid in saline research. National Research Center of Salinity, Yazd, pages 1 to 20.(In Farsi)
Qados. A., Moftah, A. E. 2015. “Influence of silicon and nano-silicon on germination growth and yield of faba bean (Vicia faba L.) under salt stress conditions”, Am J Soc Hortic Sci, 5(6): 509-524.
Rahimi, A., Shamsodin Saeed, M. and Etemadi, F. 2010.”Effects of salt stress on germination, growth and ion contents of Cumin (Nigella sativa L.)”, Arid Biom Scientific and Research Journal, 1(2): 20-31.
Rahimi, Z., Kafi, M., Nezami, A. And Khazaei, H. R. 2010. Effect of salinity and silicon levels on yield and yield of portulaca oleracea L. Iranian Journal of Agricultural Research, Volume 8, Number 3: 488-481(In Farsi).
Taarit, M.B., Msaada, K., Hosni, K., Hammami, M., Kchouk, M.E. and Marzouk, B. 2009.”Plant growth, essential oil yield and composition of sage (Salviaofficinalis L.) fruits cultivated under salt stressconditions, Industrial Crops and Products”, 30: 333-337.
Turkmen, O., S. Demir, S. Sensoy and A. Dursun. 2005. Effect of arbuscular mycorrhizal fungus and humic acidon the seedling development and nutrient content of pepper grown under saline soil conditions. J.Biol. Sci, 5(5): 565-574.
Sabaghnia, N. and Janmohammadi, M. 2015.”Effect of nano-silicon particles application on salinity tolerance in early growth of some lentil genotypes / Wpływ nanocząstek krzemionki na tolerancję zasolenia we wczesnym rozwoju niektórych genotypów soczewicy”, Annal UMCS Biol, 69(2): 39–55.
Said-Alahl, H. A. H. and Omer, E. A. 2011. Medicinal and aromatic plants production under salt stress. A review, Herba Polonica, 57 (1): 72-86.
Salimi, F., Shekari, F., Azimi, M. R. and Zangani, E. 2012 Role of methyl jasmonate on improving salt resistance through some physiological characters in German chamomile (Matricaria chamomilla L.). Iranian Journal of Plant Biology, 27: 700-711 (in Farsi).
Samavat, S. and Malakoti, M., 2006. Necessitates the use of organic acids (Humic and Fulvic) to increase the quantity and quality of agricultural products.Technical Bulletin, No. 463. Tehran.
Sangeetha, M., Singaram, P. and Uma Devi, R. 2006. Effect of lignite humic acid and fertilizer on yield of onion and nutrient availability. International Union of Soil Science.
Sato. S., Sakaguchi. S., Furukawa. H. and Ikeda. H. 2006. Effects of NaCl application to hydroponic nutrient solution on fruit characteristics of tomato (lycopersicon esculentummill.). Science Horticulture, 109, 248-253
Savvas, D., and Ntatsi, G. 2015. Biostimulant activity of silicon in horticulture. Scientia Horticulturae, 196, 66-81.
Shaaban, M., Abid, M. and Abou-Shanab, R. A. I. 2013. Amelioration of salt affected soils in rice paddy system by application of organic and inorganic amendments. Plant Soil Environment, 59: 227–233.
Shi, D. and Sheng, Y. 2004. Effect of various Salt-alkaline mixed stress conditions on sunflower seedling and analysis of their stress factors. Environmental and Experimental Botany, 49:107-120.
Valentovic, P., Luxova, M., Kolarovic, L. and Gasparikova, O. 2006. Effect of osmotic stress on compatible solutes content, membrane stability and water relations in two maize cultivars. Plant Soil and Environment, 52(4): 186–191.
Zhu, Z, Wei, G., Li, J., Qian, Q., and Yu, J. 2004. Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt-stressed cucumber (Cucumis sativus L.). Plant Science, 167:527-533.