Investigation of Morpho-physiological and Biochemical Adaptive Responses of Hovenia dulcis L. Affected by Salinity, Nitrate and Lead

Document Type : Research Paper


1 Horticultural Science Department,, Faculty of Agriculture, Islamic Azad University, Science and Research, Tehran, Iran,

2 Horticultural Science Department, Faculty of Agriculture, Islamic Azad University, Science and Research, Tehran, Iran

3 Department of Horticultural Science, Faculty of Agricultural Science and Engineering, College of Agriculture & Natural Resources, University of Tehran,

4 Soil Science Department, Faculty of Engineering and Technology, University of Tehran


This study was carried out to investigate the morpho-physiological and biochemical responses of Japanese raisin (Hovenia dulcis L.) seedlings to stress conditions of salinity (Electrical conductivity), nitrate and Pb. The experiment was conducted in a factorial experiment based on completely randomized design with three stress factors including nitrate (0, 30 and 60 mg L-1), salinity (0, 3 and 6 dSm-1) and Pb (0, 300 and 600 mg L-1) and three replications. Plants were grown under greenhouse conditions for four months. The highest shoot fresh weight was observed in treatments of 300 and 600 mg/kg Pb with zero salinity and nitrate level and the lowest fresh weight was belonged to the treatment of 6 dS/m salinity, 600 mg/kg Pb and 30 mg/L nitrate. The highest Pb concentration of shoot (72 mg kg-1 dry matter) was reported in treatment of 600 mg kg-1 Pb and with zero salinity and nitrate level. The highest value of lipids peroxidation occurred at treatment of 600 mg kg-1 Pb, indicating the plant defense mechanisms activity under these conditions. Furthermore, the synthesis of proline as a plant response to stress conditions significantly increased at 600 mg kg-1 Pb; whereas, nitrate application led to reduce malondialdehyde production in plant.


Main Subjects

Alloway, B.J. (1990) Heavy metals in soils. John Wiley & Sons, Inc . New York. USA.
Ashiri, S. and Safari, J. (2012) Nano-Au and Ag in plant beds and their application, Iranian Nanotechnology bulletin, 1(186): 1-4.
Bates, I.S., Waldern, R.P. and Tear, I.D. (1973) Rapid determination of free proline for water stressstudies.Journal of Plant and Soil, 39: 205-207.
Blum, A. (1997) Improving wheat grain filling under stress by stem reserve mobilization. In Wheat: Prospects for Global Improvement, Springer Netherlands, 135-141.
Cuisinier, O., Le Borgne, T., Deneele, D. and Masrouri, F. (2011) Quantification of the effects of nitrates, phosphates and chlorides on soil stabilization with lime and cement. Engineering Geology, 117(3-4), pp.229-235.
Emami, A. (1996) Plant analysis methods, Bulliten No.  982. Taat Publications, Tehran, Iran.
Farhangiyan-Kashani S. )2009(. The study of the effect of salinity stress on chlorophyll content in species of Medicago and onobrichis, Plant and Ecosystem, 5(18): 77-89.
Gheshlaghi, Z., Khorasani, R., Haghnia, Gh., & Kafi, M. (2015). Effect of nitrate and harvest time on yield and concentration of iron, zinc and copper in lettuce. Production and Processing of Crops and Horticulture, 5 (16), 315-330 (In Persian)
Haider G, Steffens D, Moser G, Müller C, Kammann CI. )2017( Biochar reduced nitrate leaching and improved soil moisture content without yield improvements in a four-year field study. Agriculture, Ecosystems & Environment, 237, pp.80-94.
Houda, Z., Bejaoui, Z., Albouchi, A., Gupta, D.K. and Corpas, F.J. (2016) Comparative study of plant growth of two poplar tree species irrigated with treated wastewater, with particular reference to accumulation of heavy metals (Cd, Pb, As, and Ni). Environmental monitoring and assessment, 188(2), p.99.
Husejnovic, M.S., Bergant, M., Jankovic, S., Zizek, S., Smajlovic, A., Softic, A., Music, O. and Antonijevic, B., (2018) Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2 and HaCaT). Environmental geochemistry and health, pp.1-16.
Kabata-Pendias A, Pendias H. )2000( Trace elements in soils and plants. CRC Press, Boca Raton, Florida.
Kafi, M., Borzoee, A., Salehi, M., Kamandi, A., Masoumi, A. and Nabati, J. (2015) Physiology of environmental stresses in plants, Jehat University of Ferdousi Press, 455 pp, (In Persian).
Kalhor, H., Ganji, H. and Ayati, B. (2016) Simultaneous Removal of Salinity and Organic Loading Rate using Phytoremediation, Journal of Environmental Studies, 42(3): 531-550. doi: 10.22059/jes.2016.60065
Karimi, A., Khodaverdiloo, H. and Rasouli Sadaghiani, M.H. (2017) Characterisation of growth and biochemical response of Onopordum acanthium L. under lead stress as affected by microbial inoculation, Chemistry and Ecology, 33:10, 963-976, DOI: 10.1080/02757540.2017.1391798
Karimi, A., Khodaverdiloo, H. and Rasouli Sadaghiani, M.H. (2018) Microbial‐Enhanced phytoremediation of lead contaminated calcareous soil by Centaurea cyanus L., Clean Soil Air Water, 46: 1-9:
Khadem Moqadam, N, Motesharezadeh, B. and Alikhani, H.A. (2019) The effect of zinc and potassium treatments on the antioxidant activities and physiological responses of Canola in a saline soil, Soil and Water Research, 50(60): 1409-1420, DOI: 2019.261768.667964ijswr./10.22059
Khajavi-Shojaei, S., Moezzi, A., Norouzi Masir, M., Taghavi zahedkolaei, M. (2019) Study of Ammonium and Nitrate adsorption kinetics and isotherm by common reed (Phragmites australis) biochar from aqueous solution, Iranian Journal of Soil and Water Research, 50(8), pp. 2009-2021.
Khalilpour M. and Jafarinia. A. )2017( Investigation the effects of salinity and nitric oxide on the changes of chlorophyll a fluorescence in Oat (Avena sativa L.) plant probed by JIP-Test, Iranian Journal of Plant Biology, 31: 87-98.
Khamdi F, Bakhshandeh AM  and Khamdi N. )2017( Effect of salt stress on proline content and the amount of ions, the initial growth of mung bean varieties, Quarterly Journal of Plant Production Science, 4(1): 1-4.
Khoshgoftarmanesh, A.H. (2010) Advanced concepts in plant nutrition, Isfahan University of Technology Press, No. 74, 383 p. pp: 9-12. (In Persian).
Khoshgoftar, A.H., Shariatmadari, H., Karimian, N., Kalbasi, M., van der Zee, S.E.A.T.M. and Parker, D.R. (2004) Salinity and zinc application effects on phytoavailability of cadmium and zinc. Soil Science Society of America Journal, 68: 1885-1889. doi:10.2136/sssaj2004.1885.
Matichenkov V. and Kosobrukhov, A. )2004( Si effect on the plant resistance to salt toxicity. 13th International Soil Conservation Organization Conference- Brisbane, Australia.
Momeni, A. (2011) Geographical distribution and salinity levels of soil resources of Iran, Iranian Journal of Soil Research, 24(3): 203-215,
Motesharezadeh, B., Savaghebi, G. (2012) Interaction between Cadmium and Lead and the effects of these on the concentration of Zinc and Manganese in sunflower, International Journal of Environmental Research, 6(3): 793-800. Doi: 10.22059/ijer.2012.550
Motesharezadeh, B. and Savaghebi, G.R. (2016) Phytoremediation or green remediation, University of Tehran Press, 284 pp.
Motesharezadeh, B., Navabzadeh, M., Liyaghat, A. (2016) Modeling Phytoremediation of Cadmium Contaminated Soil with Sunflower (Helianthus annus) Under Salinity Stress. International Journal of Environmental Research, 10(1): 109-118. doi: 10.22059/ijer.2016.56893
Mosleh Arani A, Rafiei A, Tabandeh A, Azimzadeh HR. (2018) Morphological and physiological responses of root and leave in Gleditschia caspica to salinity stress, Iranian Journal of Plant Biology, 9(4): 1-12.
Najafi, N., and Sarhangzadeh E. (2012) Effect of NaCl salinity and soil waterlogging on growth characteristics of forage corn in greenhouse conditions. Journal of Science and Technology of Greenhouse Culture Soilless Culture Research Center 3(2): 1-15
Poortabrizi S., pourseyedi S., Abdoshahi R., Nadernejad N. (2018) Effect of cadmium stress on morphological and physiological traits of milk thistle (Silybum marianum), Journal of Plant Process and Function, 7 (26) :185-198, URL:
Purohit, S.S. and A.K. Agrrawal. (2006) Environmental Pollution. Agrobios Pub. India.
Safarnejad A, Hamidi H. (2008) Study of morphological characters of Foeniculum vulgare under salt stress, Iranian Journal of Rangelands and Forests plants Breeding and Genetic Research, 16(1): 125-140.
Salimi M, Amin M, Ebrahimi A, Ghazifard A, Najafi P, Amini H. (2011) Influence of Salinity on Phytoremediation of Cadmium in Contaminated Soils. Journal of Health System Research, 7 (6), 1130-1137 (In Persian with abstract in English)
Saxena, G., Purchase, D., Mulla, S.I., Saratale, G.D., and Bharagava, R.N. (2019) Phytoremediation of Heavy Metal-Contaminated Sites: Eco-environmental Concerns, Field Studies, Sustainability Issues, and Future Prospects. Reviews of Environmental Contamination and Toxicology, DOI 10.1007/398_2019_24
Shabani E, Sajjadinia A, Tabatabaee S. (2015) Investigating the amount of lead tolerance in petroleum using ecophysiological properties. Journal of Science and technology of greenhouse planting. 6(23), 89-94 (In Persian with abstract in English)
Shooshtariyan, S. and Tehranifar, A. (2010) A review on phytoremediation for improvement of wastewater use, 2 nd National Congress on Water re-use and Wastewater, 10-21 (In Persian)
Siosemardeh, A., Ahmadi, A., Poustini, K. and Ebrahimzadeh, H. (2003)  Stomatal and nonstomatal limitations to photosynthesis and their relationship with drought resistance in wheat cultivars, Iranian Journal of Agricultural Science, 34(4): 93-106,
Tafvizi, M. and Motesharezadeh, B. (2014) Effects of Lead on Iron, Manganese, and Zinc Concentrations in Different Varieties of Maize (Zea mays), Communications in Soil Science and Plant Analysis,  45(14): 1853-1865,
Türkdoğan MK, Kilicel F, Kara K, Tuncer I, Uygan I. (2003) Heavy metals in soil, vegetables and fruits in the endemic upper gastrointestinal cancer region of Turkey. Environ Toxicol Pharmacol. 2003 Apr;13(3):175-9. doi: 10.1016/S1382-6689(02)00156-4.
Ullah, A., Heng, S., Munis, M.F.H., Fahad, S. and Yang, X. (2015) Phytoremediation of heavy metals assisted by plant growth promoting (PGP) bacteria: a review. Environmental and Experimental Botany, 117, pp.28-40.
Valentovic, P., Luxova, M., Kolarovi, L. and Gasparikora, O. (2006) Effect of osmotic stress oncompatible solutes content, memberane stability and water relation in two maize.Plant SoilEnvironment, 52 (4): 186-191.
Velikova,V., Yordanov, I., Edreva, A. (2000) Oxidative stress and some antioxidant
systems in acid rain-treated bean plants. Plant Science, 151(1): 59-66.
Volkmer, B.G., Ernst, B., Simon, J., Kuefer, R., Bartsch Jr., G., Bach, D. and Gschwend, J.E. (2005) Influence of nitrate levels in drinking water on urological malignancies: A community- ased cohort study. British Journal of Urology International, 95(7):
Yang, W., Wang, Y., Liu, D., Hussain, B., Ding, Z., Zhao, F. and Yang, X. (2020). Interactions between cadmium and zinc in uptake, accumulation and bioavailability for Salix integra with respect to phytoremediation, International Journal of Phytoremediation, 22(6): 628-637,
Weisany W, Sohrabi Y, Ahmadi H Abasi H. (2013) The effect of salinity stress and the application of zinc on the chlorophyll content, soluble proteins, growth, yield, and the mineral nutrients of soybean (Glycine Max L.), Plant and Ecosystem, 9(34): 75-96.
Yousefinia M, Ghasemiyan AR. (2016) Salinity stress effect assessment on photosynthesis and chlorophyll florescence a in Hordeum Vulgaris, Journal of Developmental Biology, 8(1): 35-44.