Investigation of Allicin and Flavonoid Variations and Selenium Daily Uptake in Garlic (Allium Sativum L.) By Selenium Fertilizer Treatments and Mycorrhizal Inoculation

Document Type : Research Paper


1 Ph.D. Candidate of soil chemistry, Department of Soil Science Engineering, Faculty of Agriculture, Lorestan University, Khorram Abad, Iran

2 Soil Science Department, Faculty of Agriculture, Lorestan University, Iran

3 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran


Today, numerous studies are being done to replace chemicals with natural substances in order to reduce synthetic compounds in food. Garlic (Allium sativum L.) is an important bulb vegetable because of its high concentration of allicin, flavonoids and selenium accumulation. This research was carried out to investigate the effects of two levels of mycorrhiza (inoculation and non-inoculated), three levels of selenium (0 as control, 100 and 200 mg/l) on two ecotypes (Ramhormoz and Dezful) in terms of flavonoid, allicin, daily intake of selenium and yield of garlic. The experiment was performed as factorial in the form of completely randomized design. The highest values of flavonoid (27.618 mg/g) and Allicin (5.17 mg/g) were observed in Dezful ecotype and the highest daily selenium uptake (11.08 mg/kg) was observed in Ramhormez ecotype. Mycorrhizal inoculation was significantly and positively correlated with flavonoid and garlic yield at 1% level and with allicin at 5% level, but it was reversed with daily selenium uptake (EDI) at 5 % level. Also, application of selenium fertilizer showed a positive and significant correlation with increasing daily selenium uptake and flavonoids in garlic Bulbs at 1 and 5% levels, respectively. But, it has inverse correlation with allicin content at 1 % level. Dezful ecotype had a better performance in terms of garlic weight and garlic cloves weight and there was a significant difference between two ecotypes at 1 % level. Finally, due to different effects of the treatments, if the purpose is to produce garlic enriched with Se, flavonoid or allicin, a decrease in some nutritional values and a change in ecotype must be accepted.


Main Subjects

Arzanlou, M., Bohlooli, S. (2010). Introducing of green garlic plant as a new source of allicin. Food Chemistry, 6, 12–15.
Baghalian, K., Ziaei, S. A., Naghavi, M.R., Naghdiabadi, H., and khalighi, A. (2005). Evalution of allicin contant and botanical traits in Iranian garlic (Allium sativum L.) echotypes. Sci. hort,103, 155-166.
Benkeblia, N. (2005). Free-radical scavenging capacity and antioxidant properties of some selected onions (Allium cepa L.) and garlic (Allium sativum L.) extracts. Brazilian Archives of Biology and Technology, 48, 753–759.
Braca, A., Sortino, C. Politi, M. Morelli I. and Mendez, J. (2002) Antioxidant activity of flavonoids from Licania licaniaeflora. Journal of Ethnopharmacology, 79, 379-381.
Chamannejadian, A., Sayyad, G., Moezzi, A., and Jahangiri, A. (2013). Evaluation of estimated daily intake (EDI) of cadmium and lead for rice (Oryza sativa L.) in calcareous soils. Iranian J. Environ. Health Sci. Eng, 10 (28), 1-5.
Conversa, G., Lazzizera, C., Chiaravalle A. E., Miedico, O., Bonasia, A., La Rotonda, P., Elia, Antonio. (2019). Selenium fern application and arbuscular mycorrhizal fungi soil inoculation enhance Se content and antioxidant properties of green asparagus (Asparagus officinalis L.) spears, Scientia Horticulturae, 252, 176-191.
Cuderman, P., Kreft, I., Germ, M., Kovacevic, M. and Stibilj, V. (2008). Selenium species in selenium-enriched and drought-exposed potatoes. Journal of Agricultural and Food Chemistry, 56, 9114-9120.
Evelin, H., Kapoor, R. and Giri, B. (2009). Arbuscular mycorrhizal fungi in alleviation of salt stress: A review. Annals of Botany,104. 1263–1280.
Feng, R., Wei, C. and Tu, S. (2013). The roles of selenium in protecting plants against abiotic stresses. Environmental and Experimental Botany, 87, 58-68.
Ghasemi, K., Bolandnazara, S., Tabatabaeia, S.J., Pirdashtib, H., Arzanlouc, M., Ebrahimzadehd, M. A., and Fathid, H. (2014). Antioxidant properties of garlic as affected by selenium and humic acid treatments. New Zealand Journal of Crop and Horticultural Science, 43(3), 173–181.
Golubkina, N. A., Nadezhkin, S.M., Agafonov, A.F., Kosheleva, O.V., Molchanova, A.V., Russo, G., Cuciniello, A., Caruso, G. (2019). Seed oil content, fatty acids composition and antioxidant properties as affected by genotype in Allium cepa L. and perennial onion species. Adv. Hort. Sci, 29, 199–206.
Gupta, M., and Gupta, S. (2017). An Overview of Selenium Uptake, Metabolism, and Toxicity in Plants. Frontiers in Plant Science, 7, 1-14. doi: 10.3389/fpls.2016.02074
Halliwell, B., and Gutteridge, J. M. (1990). The antioxidants of human extracellular fluids. Arch Biochem Biophys, 280(1), 1-8.
Hlusek, J., Richter, R. and Rigerova, L. (2002). Sulphur in the nutrition and fertilization of vegetables. Chemia i Inżynieria Ekologiczna, 11, 1383-1390.
Huyut, Z., Beydemir, ¸S., Gülçin, I. (2017). Antioxidant and Antiradical Properties of Selected Flavonoids and Phenolic Compounds. Biochem. Res. Int., 2017, 1-10.
Jessie Rebecca, A., Surendra Babu, P., Chandini Patnaik, M. (2018). Effect of Sulphur and Selenium on Yield, Selenium Content and Antioxidant Properties in Sunflower (Helianthus annuus L.), Int.J.Curr.Microbiol.App.Sci., 7(04): 283-289.
Juana, P., Miguel, N., Herminia, L. & Maria, C. L. (1994). Determination of Selenium Levels in vegetables and Fruits by Hydride Generation Atomic Absorption Spectrometry. J. Agric. Food Chem., 42 (12), 2848–2851.
Lawrence, R., and Lawrence, K. (2011). Antioxidant activity of garlic essential oil (Allium sativum) grown in north Indian plains. Asian Pacific Journal of Tropical Biomedicine, 1, 51–54.
Liu, C., Cooper, R.J., Bowman, D.C., (1998). Humic acid application affects photosynthesis, root development and nutrient content of creeping bentgrass, Hortscience, 33, 1023–1025.
Marrelli, M., Amodeo, V., Statti, G., and Conforti F. (2019). Biological Properties and Bioactive Components of Allium cepa L.: Focus on Potential Benefits in the Treatment of Obesity and Related Comorbidities. Molecules, 24, 119; doi:10.3390/molecules24010119.
Mollavali, M., Bolandnazar, S., Nazemieh, H., Zare, F., Aliasgharzad, N. (2015). The effect of mycorrhizal fungi on antioxidant activity of various cultivars of onion (Allium cepa L), Int J Biosci, 6:66–79.
Pizzino, G., Irrera, N., Cucinotta, M.P., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D., and Bitto, A. (2017). Oxidative Stress: Harms and Benefits for Human Health. Oxid. Med. Cell. Longev, 2017, 1–13.
Pokorný, J. (2007). Are natural antioxidants better – and safer – than synthetic antioxidants? European Journal of Lipid Science and Technology, 109(8), 883-883.
Poldma, P., Tonutare, T., Viitak, A., Luik, A. and Moor, U. (2011). Effect of selenium treatment on mineral nutrition, bulb size and antioxidant properties of garlic (Allium sativum). Journal of Agriculture and Food Chemistry, 59, 5498-5503.
Reid, M. E., Stratton, M. S., Lillico, A. J., Fakih, M., Natarajan, R., Clark, L. C. and Marshal, J. R. (2004). A report of high-dose selenium supplementation: response and toxicities. Journal of Trace Elements in Medicine and Biology, 18, 69-74.
Slusarenko, A.J., Patel, A., and Portz, D. (2008). Control of plant diseases by natural products: Allicin from garlic as a case study. Eur. J. Plant Pathol., 121, 313.
Spallholz, J. E. and Hoffman, D. J. 2002. Selenium toxicity: cause and effects in aquatic birds. Aquatic Toxicology, 57: 27-37.
Suleria, H. A. R., Butt, M. S., Khalid, N., Sultan, S., Raza, A., Aleem, Abbas, M., and Abbas M. (2015). Garlic (Allium sativum): diet based therapy of 21st century-a review. Asian Pacific journal of tropical disease, 5(4), 271-278. doi: 10.1016/S2222-1808(14)60782-9.
Varma, A., Bakshi, M., Lou, B., Hartmann, A. and Oelmueller, R. (2012). Piriformospora indica: A novel plant growth-promoting mycorrhizal fungus. Agric. Res., 1, 117-131
Wallock-Richards, D., Doherty, C. J., Doherty, L., Clarke, D. J., Place, M., Govan, J. R. W.,and Campopiano, D. J. (2014). Garlic Revisited: Antimicrobial Activity of Allicin-Containing Garlic Extracts against Burkholderia cepacia Complex. PLOS ONE, 9(12), 1-13. doi:10.1371/journal.pone.0112726.
Whanger, P. D. (2002). Selenocompounds in plants and animals and their biological significance. Journal of the American Collage and Nutrition, 21, 223-232.
White, P. J. (2016). Selenium accumulation by plants. Annals of Botany, 117(2), 217–235. doi:10.1093/aob/mcv180,
White, P. J., Bowen, H. C., Parmaguru, P., Fritz, M., Spracklen, W. P., Spidy, R. E., Meachamn, M. C., Mead, A., Harriman, M., Trueman, L. J., Smith, B. M., Thomas, B. and Broadley, M. R. (2004). Interactions between selenium and sulphur nutrition in Astragalus thaliana. Journal of Experimental Botany, 55, 1927-1937.
Zayova, E., Stancheva, I., Geneva, M., Hristozkova, M., Dimitrova, L., Petrova, M., Sichanova, M., Salamon, I., Mudroncekova, S. (2018). Arbuscular mycorrhizal fungi enhance antioxidant capacity of in vitro propagated garden thyme (Thymus vulgaris L.). Symbiosis, 74(3), 177–187.
Zhan, F., Li, B., Jiang, M., Yue, X., He, Y., Xia, Y. (2018). Arbuscular mycorrhizal fungi enhance antioxidant defense in the leaves and the retention of heavy metals in the roots of maize. Environ Sci Pollut Res Int, 25(24), 24338- 24347.