The Effect of Plant Growth Promoting Rhizobacteria Isolated from Dryland Farming on Available Phosphorus and Some Physiological and Growth Traits of Wheat under Water-Deficit Stress

Document Type : Research Paper


Department of Soli Science and Engineering, Faculty of Agricultural Engineering & Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran.


Drought, salinity and essential plant nutrient stresses especially phosphorus (P) are the most important challenges for wheat production in dryland farming of Iran. The objective of this study was to investigate the effect of three plant growth promoting bacteria strains on soil available-P, as well as some of the physiological and growth traits of wheat under water-deficit stress. For this purpose, a pot experiment was carried out as factorial arrangement with three factors including: water deficit stress at two levels, application of P-fertilizer at six levels and strains of plant growth promoting bacteria at four levels, based on completely randomized design (CRD) with three replications within 125 days. The results show at the water deficit stress of 55% field capacity (FC) and without P-fertilizer application, bacterial treatment of Staphylococcus succinus compared to control increased available-P, P-uptake of root and grain by 2.4, 4.9 and 2.7 times respectively. At moisture treatment of 80% FC and without P-fertilizer application, treatments of Bacillus pumilus, B.safensis and S. succinus compared to control, increased available-P by 1.6, 1.6 and 1.6 times; P-uptake of root by 3.1, 3.1 and 2.9 times; P-uptake of grain by 2.2, 2.4 and 2.2 times, respectively. Maximum dry weight of root, shoot and grain (5.3, 18.2 and 4.6 g pot-1, respectively) were obtained at the maximum level of P-fertilizer treatment (F4). At the water deficit stress of 55% FC, bacterial treatment of S. succinus compared to control increased prolin, root dry weight, grain dry weight and P-uptake of shoot up to 8, 31.9, 20.4 and 25.5 percent, respectively. Generally, the use of Staphylococcus succinus strain R12N2 seems to be appropriate for increasing wheat production in dryland farming.


Adnan, M., Shah, Z., Fahad, S., Arif, M., Alam, M., Khan, I. A., Mian, I. A., Basir, A., Ullah, H. and Arshad, M. (2017). Phosphate-solubilizing bacteria nullify the antagonistic effect of soil calcification on bioavailability of phosphorus in alkaline soils. Scientific Reports, 7(1), 1-13.
Ahmed, M., Khan, S., Irfan, M., Aslam, M.A., Shabbir, G. and Ahmad, S. (2018). Effect of Phosphorus on Root Signaling of Wheat under Different Water Regimes. Chapter 1, Global Wheat Production, 1-31.
Ahmadi, K., Ebadzadeh, H. R., Abdshah, H., Kazemian, A. and Rafiey, M. (2018). Agricultural Statistics Vol. I: Crops. 1st ed., Publication of Ministry of Agriculture, Deputy of Planning and Economics, Tehran, Iran. (in Farsi)
Akram, M. S., Shahid, M., Tariq, M., Azeem, M., Javed, M. T., Saleem, S. and Riaz, S., (2016). Deciphering Staphylococcus sciuri SAT-17 mediated anti-oxidative defense mechanisms and growth modulations in salt stressed maize (Zea mays L.). Frontiers in Microbiology, 7, 1-14.
Alori, E. T., Glick, B. R. and Babalola, O. O. (2017). Microbial Phosphorus Solubilization and Its Potential for Use in Sustainable Agriculture. Frontiers in Microbiology, 8, 1-8.
Awais, M., Tariqa, M., Ali, A., Ali, Q., Khan, A., Tabassum, B., Nasir, I. A. and Husnain, T. (2017). Isolation, characterization and inter-relationship of phosphate solubilizingbacteria from the rhizosphere of sugarcane and rice. Biocatalysis and Agricultural Biotechnology, 11, 312–321.
Barnawal, D., Bharti, N., Pandey, S. S., Pandey, A., Chanotiya, C. S. and Kalra, A. (2017). Plant growth‚Äźpromoting rhizobacteria enhance wheat salt and drought stress tolerance by altering endogenous phytohormone levels and TaCTR1/TaDREB2 expression. Physiologia Plantarum, 161(4), 502-514.
Bates, S., Waldern, R. P. and Teare, I. D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil, 39(1), 205-207.
Blaise, D., Venugopalan, M. V. and Singh, G. (2018). Textbook of Plant Nutrient Management, Phosphorus Management, 93-121.
Canarini, A., Kaiser, C., Merchant, A., Richter, A. and Wanek, W. (2019). Root Exudation of Primary Metabolites: Mechanisms and Their Roles in Plant Responses to Environmental Stimuli. Frontiers in Plant Science, 10, 1-19.
Delfim, J., Schoebitz, M., Paulino, L., Hirzel, J. and Zagal, E. (2018). Phosphorus Availability in Wheat, in Volcanic Soils Inoculated with Phosphate-Solubilizing Bacillus thuringiensis. Sustainability, 10, 1-15.
Etesami, H., and Maheshwari, D. K. (2018). Use of plant growth promoting rhizobacteria (PGPRs) with multiple plant growth promoting traits in stress agriculture: Action mechanisms and future prospects. Ecotoxicology and Environmental Safety, 156, 225–246.
Huang, B., North, G. and Nobel P. S. (1993). Soil sheaths, photosynthate distribution to roots and rhizosphere water relations for Opuntia ficus-indica. International Journal of Plant Sciences, 154(3), 425-431.
Hunter, P. J., Teakle, G. R. and Bending, G. D. (2014). Root traits and microbial community interactions in relation to phosphorus availability and acquisition, with particular reference to Brassica. Frontiers in Plant Science, 5, 1-18.
Inwati, D. K., Yadav, J., Yadav, J. S., Pandey, G. and Pandey, A. (2018) Effect of different levels, sources and methods of application of nitrogen on growth and yield of wheat (Triticum aestivum L.). Int J Curr Microbiol App Sci, 7, 2398-2407.
Jeshni, M. G., Mousavinik, M., Khammari, I. and Rahimi, M. (2017). The changes of yield and essential oil components of German Chamomile (Matricaria recutita L.) under application of phosphorus and zinc fertilizers and drought stress conditions. Journal of the Saudi Society of Agricultural Sciences, 16, 60–65.
Johnston, A. E. and Syers, J. K. (1998). Nutrient management for sustainable crop production in Asia. Wallingford, UK, CAB International, 394 pp.
Kadmiri, I. M., Chaouqui, L., Azaroual, S. E., Sijilmassi, B., Yaakoubi, K. and Wahby, I. (2018). Phosphate-Solubilizing and Auxin-Producing Rhizobacteria Promote Plant Growth under Saline Conditions. Arabian Journal for Science and Engineering, 43(7), 3403–3415.
Kaur, G. and Reddy, M. S. (2015). Effects of Phosphate-Solubilizing Bacteria, Rock Phosphate and Chemical Fertilizers on Maize-Wheat Cropping Cycle and Economics. Pedosphere, 25(3), 428–437.
Kaushal, M. and Wani, S. P. (2016). Rhizobacterial-plant interactions: Strategies ensuring plant growth promotion under drought and salinity stress. Agriculture, Ecosystems and Environment, 231, 68-78.
Klute, A. (Ed), (1986). Methods of Soil Analysis. Part 1: Physical and Mineralogical Methods. 2nd ed. Agronomy, ASA and SSSA, Wisconsin, USA: Madison.
Lorck, H. (1948). Production of hydrocyanic acid by bacteria. Physiol Plant, 1, 142–146.
Malekutey, M. J. and Gheybi, M. N. (1997). Determination of the critical level of the nutritional elements in strategic products and the correct recommendation of fertilizer in the country. Agriculture education publication, Karaj. (in Farsi)
McBeath T. M., McLaughlin M. J., Kirby J. K., and Armstrong R. D. 2012. The effect of soil water status on fertiliser, topsoil and subsoil phosphorus utilisation by wheat. Plant and Soil, 358(2), 337–348.
Michel, B. E. and Kaufmann, M. R. (1973). The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51(5), 914–916.
Moreira, H., Pereira, S. I. A., Marques, A. P. G. C., Rangel, A. O. S. S. and Castro, P. M. L. (2019). Effects of soil sterilization and metal spiking in plant growth promoting rhizobacteria selection for phytotechnology purposes. Geoderma, 334, 72–81.
Oksinska, M. P., Wright, S. A. I. and Pietr, S. J. (2011). Colonization of wheat seedlings (Triticum aestivum L.) by strains of Pseudomonas spp. with respect to their nutrient utilization profiles. European Journal of Soil Biology, 47(6), 364-373.
Ova, E. A., Kutman, U. B., Ozturk, L. and Cakmak, I. (2015). High phosphorus supply reduced zinc concentration of wheat in native soil but not in autoclaved soil or nutrient solution. Plant and Soil, 393(2),147–162.
Ozturk, L., Eker, S., Torun, B. and Cakmak, I. (2005). Variation in phosphorus efficiency among 73 bread and durum wheat genotypes grown in a phosphorus-deficient calcareous soil. Plant and Soil, 269(2), 69-80.
Page A. L., Miller, R. H. and Keeney, D. R. (Eds.), (1982). Methods of Soil Analysis. Part 2: Chemical and Microbiological Properties. 2nd ed. Agronomy, ASA and SSSA, Wisconsin, USA: Madison.
Patten, C. L. and Glick, B. R. (2002). Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Appl. Environ. Microbiol., 3795–3801.
Penrose, D. M. and Glick, B. R. (2003). Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiol Plants, 118, 10-15.
Pinton, R., Varanini, Z. and Nannipieri, P. (2007). The Rhizosphere Biochemistry and Organic Substances at the Soil-Plant Interface. CRC Press Taylor & Francis Group, LLC.
Rai, A., Cherif, A., Cruz, C. and Nabti, E. (2018). Extracts from Marine Macroalgae and Opuntia cus-indica Cladodes Enhance Halotolerance and Enzymatic Potential of Diazotrophic Rhizobacteria and Their Impact on Wheat Germination under Salt Stress. Pedosphere, 28, 241–254.
Rashid, M., Khalil, S., Ayub, N., Alam, S. and Latif, F. (2004). Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms (PSM) under in vitro conditions. Pakistan Journal of Biological Sciences, 7(2), 187–196.
Razzaghi, B. K., Alikhani, H. A., Etesamia, H. and Khoshkholgh-Sima, N. A. (2019). Improved growth and salinity tolerance of the halophyte Salicornia sp. by co–inoculation with endophytic and rhizosphere bacteria. Applied Soil Ecology, 138, 160–170.
Rodriguez, D. and Goudriaan, J. 1995. Effects of phosphorus and drought stresses on dry matter and phosphorus allocation in wheat. Journal of Plant Nutrition, 18(11), 2501-2517.
Rodriguez, D., Goudriaan, J., Oyarzabal, M. and Pomar, M. C. 2008. Phosphorus nutrition and water stress tolerance in wheat plants. Journal of Plant Nutrition, 19(1), 29-39.
Salem, G., Stromberger, M. E., Byrne, P. F., Manter, D. K., El-Fekid, W. and Weir, T. L. (2018). Genotype-specific response of winter wheat (Triticum aestivum L.) to irrigation and inoculation with ACC deaminase bacteria. Rhizosphere, 8, 1–7.
Saleemi, M., Kiani, M. Z., Sultan, T., Khalid, A. and Mahmood, S. (2017) Integrated effect of plant growth-promoting rhizobacteria and phosphate-solubilizing microorganisms on growth of wheat (Triticum aestivum L.) under rainfed condition. Agri Food Secur, 6:46.
Sandhya, V., Ali, S. K. Z, Minakshi, G., Reddy, G. and Venkateswarlu, B. (2009). Alleviation of drought stress effects in sunflower seedlings by the exopolysaccharides producing Pseudomonas putida strain GAP-P45. Biology and Fertility of Soils, 46(1), 17–26.
Sarikhani, M. R., Khoshru, B. and Oustan, S. (2016). Efficiency of some bacterial strains on potassium release from micas and phosphate solubilization under in-vitro conditions. Geomicrobiol. J., 33, 832–838.
Schwyn, B. and Neilands, J. B. (1987). Universal chemical assay for the detection and determination of siderophores. Anal Biochem, 160, 47-56.
Sharma, S. B., Sayyed, R. Z., Trivedi, M. H. and Gobi, T. A. (2013). Phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils. Springer Plus, 2, 587.
Sperber, J. I. (1958). The incidence of apatite-solubilizing organisms in the rhizosphere and soil. Australian Journal of Agricultural Research, 9(6), 778 – 781.
Stamford, N. P., Santos, P. R., Moura, A. M. M. F. and Freitas, A. D. S. (2003). Biofertilizers with natural phosphate, sulphur and Acidithiobacillus in a soil with low available-P. Scientia Agricola, 60(4), 767–773.
Sultenfuss, J. H. and Doyle, W. J. (1999). Phosphorus for Agriculture. Better Crops, 83, 1-40.
Upadhyay S. K., Singh, J. S., and Singh, D. P. (2011). Exopolysaccharide-Producing plant growth-promoting rhizobacteria under salinity condition. Pedosphere, 21(2), 214– 222.
USDA. (2019). World Agricultural Production. Foreign Agricultural Service. Circular Series WAP, 5-19.
Vurukonda, S. S. K. P., Vardharajula, S., Shrivastava, M. S. Z. A. (2016) Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiological Research, 184, 13-24.