Biodegradation of Kerosene by Native Bacteria Isolated from Vegetated and Kerosene Contaminated Fields' Soil in Khuzestan Province

Document Type : Research Paper

Authors

1 Department of Soil Science,Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Ahvaz, Iran.

2 Department of Plant Protection,Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Ahvaz, Iran.

3 Department of Biology; Research center of Biotechnology and biological Sciences, Shahid Chamran University of Ahvaz.

Abstract

The present study was conducted to investigate the possibility of growth of native bacteria isolated from some kerosene-contaminated soils in Khuzestan province for degradation of oil spills. After the primary stages of enrichment, isolation, and screening, bacteria were identified based on the gene sequences of 16S rRNA gene. The ability of native bacteria as the bacteria using hydrocarbons in the presence of oil products such as kerosene and crude oil at 1% concentration of solid and liquid minimum salt medium (MSM) was measured. A split-plot experiment with a factorial design was conducted with factors such as oil (kerosene and crude oil), bacteria, and time in three replicates, using the microbial inoculation inoculum (with a population of 108 CFU/ml) in both liquid and solid growth environments. The degradation ability of hydrocarbons in solid and liquid growth environments was investigated using colony diameter assessment and turbidity, respectively. In this research, five bacteria belonging to the genera of Microbacterium phyllosphaerae (ZS1.9), Bacillus megateriuns (ZS2.12), Staphylococcus epidermidis (ZS3.170), Streptomyces albogriseolus (ZS4.9), and Bacillus subtilis (ZS5. 210) were identified. The results revealed that the bacteria had the highest and lowest growth in the presence of kerosene and crude oil, respectively. Among the oil products used in this study, kerosene had the highest degradation rate. Furthermore, the ability of bacteria for degradation oil products increased by increasing incubation period. The findings of this study revealed the ability of different bacterial species in bioremediation of environmental pollution to various hydrocarbons.

Keywords

Main Subjects

References

Adeli, M., Shahian, M.A. And Karimi Nick, A. (2013). Isolation, Identification and Characterization of Two Shiquan Species of the Biosurfactant Producer from the Persian Gulf. The World of Germs, 6(1). (In Farsi)
Akpoveta, O.V., Egharevba, F. and Medjor, O.W. (2011). A pilot study on the biodegradation of hydrocarbon and its kinetics on kerosene simulated soil. International. Journal of Environmental Sciences., 2(1): 54-67.
Atlas, R.M. (1981). Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiol, Rev., 45: 180-209.
Bayat, Z., SHaheyan, M.H. and Askari, M. (2015). Isolation and identification of crude petroleum degrading bacteria from the Haustrum scobina, collected from the Persian Gulf (Bandar Abbas coastal area). Quarterly Journal of Biological Microorganisms., 5(17): 61- 72. (In Farsi)
Cabello, M.N. (1997). Hydrocarbon pollution: its effect on native arbuscular mycorrhizal fungi (AMF). FEMS Microbiology Ecology., 22: 233–236.
Das, R. and Tiwary, B.N. (2014). Production of indole acetic acid by a novel bacterial strain of Planomicrobium chinense isolated from diesel oil contaminated site and its impact on the growth of Vigna radiata. European Journal of Soil Biology., 62: 92-100.
Ebrahimi Pour, GH. H., Marzban, A.A., karkhanh, M. And Fakhary, C. (2012). Investigation of biological removal of oil hydrocarbon contaminants by isolated bacteria from oil contaminated soils. Journal of Microbiological Biotechnology, Islamic Azad University., 4 (13): 13-20. (In Farsi)
Ebrahimi, M., Sarikhani, M. R. And Fallah, A.R. (2013). Investigation of Biodegradation of Petroleum Gas, Toluene and Phenanthrene Oil by Three Pantoea P and 5 Pseudomonas putida P13, Pseudomonas fluorescens CHAO agglomerans. Journal of Water and Soil Science., 23(2): 29-41. (In Farsi)
Emtiazi, G., Shakarami, H., Nahvi, I. and Mirdamadian, SH. (2005). Utilization of petroleum hydrocarbons by Pseudomonas sp. and transformed Escherichia coli. African Journal of Biotechnology., 4(2): 172-176.
Hamman, S. (2004). Bioremediation capabilities of white rot fungi. BI570-review article.
Jafari Hafsjani, A., Asadi Koorrchal, S.H., Omaye, M. and Bayborde, M. (2016). Journal of Soil Management and Sustainable Production., 6(4): (In Farsi)
Khodaveisi, V. (2012). Effect of soil pollution on kerosene on physiological characteristics and growth of some vegetables. Master's degree in Agricultural and Natural Resources University Ramin Khuzestan. (In Farsi)
Mohsenzadeh, F. (2011). Investigating the Effluent Efficiency of Oil Pollutants by Native Bacterial Strains Isolated from Polluted Soils in Cooling Areas (Case Study: Tabriz Refinery) Journal of Cellular and Molecular Research. Journal of Biology., 27(3). (In Farsi)
Olalemi, A.S. and Arotupin, D.J. (2012). Effect of refined petroleum contamination on bacterial population and physicochemical characteristics of cultivated Agricultural soil. Journal of microbiology, Biotechnology and Food Sciences., 2(2): 684-700.
Rahman, K.S., Thahira-Rahman, J., Lakshmanaperumalsamy, P. and Banat, I.M.(2002). Towards efficient crude oil degradation by a mixed bacterial consortium. Bioresource technology., 85(3): 257- 261
Schaad, N.W., Joens, J.B. and Chun, W. (2001). Laboratory Guide for Identification of Plant Pathogenic Bacteria. A.P.S. USA: .373.
Sparks, D.L. (2003). Environmental Soil Chemistry. 2ed Academic Press. California. USA.
Subathra, M.K., Immanuel, G.and Suresh, A.H. (2013). Isolation and Identification of hydrocarbon degrading bacteria from Ennore creek. Bioinformation.,9:150
Udgire, M., Shah, N. and Jadhav, M. (2015). Enrichment, Isolation and Identification of Hydrocarbon Degrading Bacteria International. Journal of Current Microbiology and Applied Sciences.,4: 708-713.
Wang, J., Zhang, Z., Li, Y., Zhang, B.and Zhang, G. (2015). Cold‐adapted bacteria for bioremediation of crude oil‐contaminated soil. Journal of Chemical Technology and Biotechnology., 91: 2286-2297.
Weisburg, W.G., Barns, S.M. and Pelletier, D.A. (1991). 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology., 173: 697–703.
Winnepenninckx, B., Backeljau, T.and Dewachter, R. (1993). Extraction of high molecular weight DNA from molluscs. National Institutes of Health., 9: 407.
Wongsa, P., Tanaka, M., Ueno, A., Hasanuzzaman, M., Yumoto, I. and Okuyama, H. (2004). Isolation and Characterization of Nonel Strains of Pseudomonas aeruginosa and Serratia marcescens possessing High Efficiency to Degrade Gasoline, Kerosene, Diesel oil, and Lubricating Oil. Current Microbiology., 49: 415-422.
Iranian Journal of Soil and Water Research
Volume 50, Issue 7
December 2019
Pages 1747-1757

Files

Share

How to cite

Statistics