Influence of Leaf Nutrition of Some Micronutrients on Absorption of Mentha Plant under Soil Phosphorus Deficiency

Document Type : Research Paper


1 MSc student, Department of Medicinal Plants, Shahid Bakeri High Education Center of Miandoab, Urmia University, Urmia, Iran

2 Assistant Professor, Department of Medicinal Plants, Shahid Bakeri High Education Center of Miandoab, Urmia University, Urmia, Iran

3 Assistant Professor, Department of Soil Science, Shahid Bakeri High Education Center of Miandoab, Urmia University, Urmia, Iran


Phosphorus is one of the most used fertilizer macronutrients and in calcareous soils of Iran, its absorption is limited. On the other hand, considering the problems caused by the soil use of micronutrients, it is possible to modify the effects of phosphorus deficiency by spraying micronutrients. This study was conducted as factorial in a completely randomized design in three replications to investigate the effect of leaf nutrition of micronutrients (iron, zinc and copper) on the absorption of nutrients of Mentha aquatica L. in phosphorus deficiency conditions. Experimental treatments consisted of no deficiency (control) and two levels of phosphorus deficiency and foliar application of Fe2+, Zn2+ and Cu2+ in concentration of 1 and 2%. Deficient plants showed a significant decrease in P content of root. Spraying Fe2+ and Cu2+ 2% caused increase in P content under deficiency. Foliar nutrition with iron, zinc and copper caused decrease in nitrogen and phosphorus content in deficient plants. Applying zinc and iron as spraying caused increase in NO3content in shoots and roots. In the present study, spraying zinc, iron and copper caused increase in their contents in shoots and roots of Mentha. Spraying Fe2+ 2% caused increase in P, NO3 and Fe2+ contents in shoots of Mentha plant. The orthogonal comparison results showed that there was a significant difference in nutrients content between foliar application of micronutrients and control and also between foliar application of iron compare to copper and zinc and foliar application of zinc compared to copper in shoots and roots of Mentha. Regarding the obtained results, leaf nutrition of micronutrients as spraying can be mentioned as a suitable suggestion to reduce the effects of macronutrients deficiency, especially phosphorus. It seems that spraying iron and zinc showed the highest effect in absorption of other nutrients in shoots, also spraying copper had the highest effect in roots.


Main Subjects

Ameziane, R., Cassan, L., Duffosse, C., Ruffy, T. W. and Limami, A. M. (1997). Phosphate availability in combination with nitrate availability affects root yield and chicon yield and quality of Belgain endive (Cicorium intybus). Plant and Soil, 191, 269-277.
Asgari Lajayer, H. Savaghebi Firoozabadi, G. R. Motesharezadeh, B. and Hadiyan, J. (2015). Change in uptake of micronutrient and macronutrient in pennyroyal (Mentha pulegium L.) at greenhouse condition under copper and zinc application. Journal of Soil Management and Sustainable Production, 5(2), 197-210.
Auld, D. S. (2001). Zinc coordination sphere in biochemical zinc sites. Biometals. 14, 271–313.
Bremner, J. M. (1965). Inorganic forms of nitrogen. In C.A. Blank (Ed.). Methods of soil analysis, (Part. 2). (pp. 1179–1232). ASA and SSSA, Wisconsin: Madison.
Bron, J. C., Amblers, J. E., change, R. L. and Foy, C. D. (2003). Differential response of plant genotype to micronutrients. In J. Mortvedt et al (Ed.). Micronutrients in an agriculture soil science society of America in corporation, (NO. 2). (pp. 389-418). Wisconsin: Medison.
Cataldo, D. A., Haroon, M., Schrader, L. E. and Youngs, V. L. (1975). Rapid colorimetric determination in plant tissues by nitration of salisylic acid. Communications in Soil Science and Plant Analysis, 6, 71–80.
Chrysargyris, A., Panayiotou, C. and Tzortzakis N. (2016). Nitrogen and phosphorus levels affected plant growth, essential oil composition and antioxidant status of lavender plant (Lavandula angustifolia Mill.). Industrial Crops and Products, 83, 577-586.
Esmaeili, A., Rustaiyan, A., Masoudi, S. and Nadji, K. (2006). Composition of the Essential Oils of Mentha aquatica L. and Nepeta meyeri Benth from Iran. Journal of Essential oil Research, 18(3), 263-265.
Gee, G. W. and Baulder, J. W. (1986). Particle- size analysis. In C.A. Blank (Ed.). Methods of soil analysis, (Part. 1). (pp. 383-411). ASA and SSSA, Wisconsin: Madison.
Gupta, V. ­K. and Singh, B. (1985). Residual effect of zinc and magnesium on maize crop. Journal of the Indian Society of Soil Science, 23, 204 -207.
Hawkesford, M., Horst, W., Kichey, T., Lambers, H., Schjoerring, J., Skrumsager Moller, I. and White, P. (2012). Functions of macronutrients. In H. Marschner (Ed.). Mineral nutrition of higher plants, (No. 2). (pp. 135-189)..Academic Press: London.
 Hoagland, D. R. and Arnon, D. I. (1950). The water culture method for growing plant without soil. University of California Berkley Press, College of Agriculture, Agricultural Experiment Station, p. 347.
Jacob, J. and Lawlor, D.W. (1992). Dependence of photosynthesis of sunflower and maize on phosphate supply, ribulose-1, 5-biphosphate carboxylase/ oxygenase activity, and ribulose1,5-biphosphate pool size. Plant Physiol. 98: 801–807.
Jamzad, Z. (2012). Iran flora (Lamiaceae family). Research Institute of Forests and Rangelands (RIFR): Iran. (In Farsi)
Lindsay, W. L. and Norvell, W. A. (1979). Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Science Society American Journal, 42, 421–428.
Lotfollahi, M. (2003). The role of micronutrients in increasing yield and micronutrients with a large impact. Soil Science Department, Tarbiat Modarres University Press, Tehran, Iran. (In Farsi)
Malakoti, M. J. and Homaei, M. (2004). The Arid Soil Fertility "Solve Problems and Ways". Second edition. Tarbiat Modarres University Press.
Malakouti, M. J. and Tehrani, M. M. (1999). The role of micronutrients on increasing the yield and quality of agricultural products. Tarbiat Modarres University Press, Tehran, Iran. (In Farsi)
Marrin, M. Koko, V., Duletic-Lausevic, S., Marin, P. d., Rancic, D. and Dajic-Stevanovi, C. (2007). Glandular trichomes on the leaves of Rosmarinus officinalis: morphology, stereology and histochemistry. South African Journal of Botany, 72, 378–382.
Marschner, H. (1986). Mineral Nutrition of Higher Plants. Academic Press, London.
Marschner, H., (1995). Mineral Nutrition of Higher Plants. Second edition, Academic Press. Harcourt Brace and Company, Publishers, London, pp. 347-364.
Marschner, H. (2011). Mineral Nutrition of Higher Plants. Academic Press, New York, p. 899.
McLean, E.O. (1982). Soil pH and Lime requirement. In. C. A. Blank (Ed.). Methods of soil analysis, (Part 2). (pp. 199-224). ASA and SSSA, Wisconsin: Madison.
Moghimi pour, Z., Mahmoudi Sourestani, M. and Alamzadeh Ansari, N. (2016). The influence of foliar application of nano zinc chelate and zinc sulfate on morphological traits of holy basil (Ocimum sanctum), indices and photosynthetic pigments. Journal of Horticultural Science, 2, 242-250. (In Farsi)
Mohammadi pour, R., Sedaghathoor, S. and Mahboub Khomami, A. (2013). Effect of application of iron fertilizers in two methods 'foliar and soil application' on growth characteristics of Spathyphyllum illusion. Iranian Journal of Horticulture Science Technology, 3(1), 232-240.
Mortvedt, G. G., Giordana, P. M. and Lindsay, W. L. (1972). Micronutrients in agriculture. Soil Science Society of American, Madison: Willey.
Movahhedy-Dehnavi, M., Modarres Sanavy, S. A. and Mokhtassi Bidgoli, A. (2009). Foliar application of zinc and manganese improves seed yield and quality of safflower (Carthamus tinctorius L.) grown under water deficit stress. Industrial Crop Production, 30(1), 82-92.
Mousa, G. T., El-Sallami, I. H. and Ali, E. F. (2003). Response of Nigella sativa L. to foliar application of gibberellic acid, benzyladenine, iron and zinc. Assist Journal of Agricultural of Science, 32, 141-156.
Murphy, J. and Riley, J. P. (1962). A modified single solution method for the determination of phosphate in natural waters. Analytica Chimica, 27, 31-36.
Nourgholipour, F., Mirseyed Hosseini, H., Tehrani, M.M., Motesharezadeh, B. and Moshiri, F. (2018). Response to phosphorus deficiency stress among winter rapeseed (Brassica napus L.) cultivars. Iranian Journal of Soil and Water Research, 94(3), 515-525.
Olamaei, V., Ronaghi, A. M., Karimian, N. A., Yasrebi, J., Hamidi, R. and Tavajjoh, M. (2013). Comparison of yield, yield components and seed quality (oil and protein content) of two rapeseed cultivars as affected by different levels of soil-applied nitrogen and zinc. Journal of Science and Technology Greenhouse Culture, 4­(16), 83-98. (In Farsi)
Olsen, S. R., Cole, C. V., Watanabe, F. S. and Dean, L. A. (1954). Estimation of available phosphorus in soil by extraction with sodium bicarbonate. USDA Circ. Government Print Office: Washington.
Öncel, I., Keleş, Y. and Üstün, A. (2000). Interactive effects of temperature and heavy metal stress on the growth and some biochemical compounds in wheat seedlings. Environmental Pollution, 107(3), 315-320.
Pahlavan Rad, M., Keykha, G. and Naroui Rad, M. (2008). Effects of application of Zn, Fe and Mn on yield, yield components, nutrient concentration and absorption in wheat grain. Pajouhesh and Sazandegi, 79, 142-150. (In Farsi)
Purnomo, E. and Black, A. S. (1994). Phosphorus fertilizer as affected by time and method of application in soil with acidic subsurface layer. Fertilizer Research, 39, 77-82.
Ravi, S., Channal, H. T., Hebsur, N. S., Patil, B. N. and Dharamtti, P. R. (2008). Effect of sulfur, zinc and iron nutrition on growth, yield, nutrient uptake and quality of safflower (Carthamus tinctorius L.). Karnataka Journal of Agricultural Sciences, 21, 382-385.
Roades, J. D. (1990). Soluble salts. In C. A. Blank (Ed.). Methods of soil analysis. (Part. 2). (pp. 167-179). ASA and SSSA, Wisconsin: Madison.
Roosta, H. and Mohsenian Y. (2012). Effects of foliar spray of different Fe sources on pepper (Capsicum annum L.) plants in aquaponic system. Scientia Horticulturae, 146,182–191.
Salim, N. 2000. Symptoms of nutrient deficiency in fruit trees. Management of Agricultural Promotion, First Edition. 10-13p.
Seadh, S. E., El-Abady, M. I., El-Ghamry, A. M. and Farouk, S. (2009). Influence of micronutrients foliar application and nitrogen fertilization on wheat yield and quality of grain and seed. Journal Biological Sciences, 9­(8), 851-858.
Sial, N. B., Rajpar, I. and Solangi, S. (2005). Effects of foliar application of Zn on growth, yield and fiber characters of two cotton (Gossypium Hirsutum L.) varieties. Pakistan Journal of Agricultural Engineering and Veterinary Sciences, 21, 11-16.
Taylor, M. D., Nelson, P. V., Frantz, J. M. and Rufty, T. W. (2010).Phosphorus deficiency in pelargonium: effects on nitrate and ammonium uptake and acidity generation. Journal of Plant Nutrition, 33, 701-712.
Thiruppathi, M. K., Thanunathan, K., Ganapathy, M., Prakash, M. and Imayavaramban, V. (2001). Nutrient uptake and quality characters of sesame (Sesam unindicum L.) as influenced by micronutrients, biofertilizer and phytohormones. Sesame and Safflower News, 16, 51-56.
Walker, R. R., Blackmore, D. H., Clingeleffer, P. R. and Correll, R. L. (2004). Rootstock effects on salt tolerance of irrigated field-grown grapevines (Vitis vinifera L. cv. Sultana) 2. Ion concentrations in leaves and juice. Australian Journal of Grape and Wine Research, 10, 90-99.
Walkley, A. and Black, L. A. (1934). Examination of the Degtjareff method for determining soil organic matter and a proposed modification of chromic acid titration method. Journal of Soil Science, 37, 29-38.
Yassen, A., Abou El-Nour, E. A. A. and Shedeed, S. (2010). Response of wheat to foliar spray with urea and micronutrients. Journal of American Science, 6, 14-22.
Zhang, F. S. (1993). Effect of Cu deficiency on Zn uptake rate of wheat plants­. Acta-Pedologica Sinica, 235, 129-134.