Effect of Mycorrhizal Fungi and Phosphate Solubilizing Bacteria on Corn Growth and Cadmium Uptake in Cadmium Spiked Soils

Document Type : Research Paper

Authors

1 MSc of Soil Biology, Department of Soil Science, Faculty of Agriculture, University of Zanjan

2 Professor of Soil Science, Faculty of Agriculture, Zanjan University

3 Ph.D. student of Agronomy, Department of Agronomy, Faculty of Agriculture, Zanjan University

Abstract

In order to investigate the effect of bio-fertilizers on contamination rate of plants to heavy metals, a factorial experiment based on completly randomized design with three replications was carried out in greenhouse of soil science department at agriculture faculty of Zanjan University in 2015. The proposed treatments were: contamination levels of soil to cadmium (0, 10, 25, 50 and 100 mg of cadmium per Kg of soil) and inoculation with bio-fertilizers including; phosphate solubilizing bacteria Pseudomonas putida, mycorhiza fungi Funneliformis mosseae and Rhizophagus intraradices. The measured factors were leaf chlorophyll index, plant height, shoot and root fresh and dry weight, phosphorus and potassium of shoot and root and cadmium concentration in plant. The results indicated that the use of bio-fertilizers increased the leaf chlorophyll index, plant height, shoot and root fresh and dry weight, phosphorus and potassium of shoot and root significantly as compare to the control. The treatment of Funneliformis mosseae and Pseudomonas putida (M+P) could improve leaf chlorophyll index and plant height by 11.93 and 21.89 % in comparing whit control respectively. The chlorophyll index was significantly decreased by increasing cadmium levels in the soil. Simultaneous application of Funneliformis mosseae and Pseudomonas putida (M+P) and cadmium increased the shoot and root dry weight by 6 and 7 % as compared to treatment of 100 mg cadmium per Kg of soil. Totally, the results indicated that the inoculation of soil whit bio-fertilizers could reduce the harmful effects of cadmium on plants growth and yield.

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Main Subjects

References

Adewole, M. B. Awotoye, O. O. Ohiembor, M. O. and Salami, A. O. 2010. Influence of mycorrhizal fungi on phytoremediation potential and yield of sunflower in cd and Pb polluted soils. Jornal Agricultural Science. 55: 17-28.
Andrade, S. A. L. Abreu, C. A. De Abreu, M. F. and Silveira, A. P. D. 2004. Influence of lead additions on arbuscular mycorrhiza and Rhizobium symbioses under soybean plants. Applied Soil Ecology, 26(2), 123-131.
Azcon, R. and El-Atrash, F. 1997. Influence of Arbuscular mycorrhizae and phosphorus fertilization on growth , nodulation and fixation (N15) in Medicago sativa at four salinity levels. Biology and Fertility of Soils. 24: 81-86.
Bashan, Y. and Holguin, G. 1997. Azospirillum–plant relationships: environmental and physiological advances (1990–1996). Canadian Journal of Microbiology, 43(2), 103-121.
Brooks, R. R. Lee, J. Reeves, R. D. and Jaffre, T. 1977. Detection of nickeliferous rocks by analysis of herbarium specimens of indicator plants. Journal of Geochemical Exploration. 7: 49-57.
Cooper, E. M. Sims, J. T. Cunningham, S. D. Huang, J. W. and Berti. W. R. 1999. Chelate-Assisted Phytoextraction of lead from contaminated soils. Jornal of Environmental Quality. 28: 1709-1719.
Das, P. Samantaray, S. and Rout, G. R. 1997. Studies on cadmium toxicity in plants: a review. Environ. Poll. 98: 29-36.
Demir, S. 2004. Influence of arbuscular mycorrhiza on some physiological growth parameters of pepper. Turkish Journal of Biology, 28(2-4), 85-90.
Ehyaei, M., And A.S. Behbahani Zadeh,. 1993. Descriptions of Soil Chemical Analysis Methods, No. 893, Soil and Water Research Institute, Tehran.
Gamalero, E. Trotta, a. Massa, N. Copetta a, a. Martinotti, M. G. and Berta, G. 2004. Impact of two fluorescent pseudomonads and an arbuscular mycorrhizal fangus on tomato plant growth root architecture and P acquisition. Mycorrhiza 14:185-192.
Goyanrajlo. M. Cole, C. V. watanabe . F. S.  and Dean, L. A. 2005. Effect of cadmium toxicity on nitrogen and phosphorus uptake and some vegetative characteristics of shoots of seven rice cultivars. Science and technology of greenhouse cultivation. 4 (8): 125-142.
Gee, G. W. and Bauder, J. W. 1986. Particle-size analysis. Methods of soil analysis: Part 1—Physical and mineralogical methods, (methodsofsoilan1), 383-411.
Ghahramani, R. 2008. Investigation of the severity of pollutants in southern Tehran to cadmium and its absorption by spinach. Master thesis, Department of Soil Science, Faculty of Agriculture, Tarbiat Modarres University, Iran.
Glick, B. R. Penrose, D. M., and Li, J. 1998. A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria. Journal of theoretical biology, 190(1), 63-68.
Golchin, A. and shafiee, S. 2006. Study the effect of zanjan lead-zinc factor on the soil pollution up to 10 km from the factory. Soil, environment andsustainable development congress, Karaj, Iran. Pp: 452-454. (In Persian).
Golchin, A., Atashnama, K., and M., Takasi. 2006. Investigating the distribution of lead in various organs of sunflower and rapeseed as an oil producer. Pages 306-305. Proceedings of Soil Conference, Environment and Sustainable Development, Pardis Publishing House, Tehran University of Agriculture and Natural Resources, Karaj, Iran.
Gouia, H., Ghorbal M. H. and Meyer, C. 2001. Effect if cadmium on activity of nitrate reductase and on other enzymes of the nitrate assimiltion pathway in bean. Plant Physiology, 38: 629-638.
Haghiri, F. 1973. Cadmium uptake by plants. Journal of Environmental Quality, 2(1), 93-95.
Hall, J. L. 2002. Cellular mechanisms for heavy metal detoxification and tolerance. Journal of experimental botany, 53(366), 1-11.
Heggo, A. Angle, J. S. and Chaney, R. L. 1990. Effects of vesicular arbuscular mycorrhizal fungi on heavy metal uptake by soybeans. Soil Biology and Biochemistry. 22: 865-869.
Horst, V. 2004. Further root colonization by arbuscular mycorrhizal fungi in already mycorrhizal plants in suppressed after a critical level of root colonization. Journal of Plant Physiology. 161:339 -341.
Jeffries, P. 2001. Achievements in the past and autlook for the future of AMF. Research School of Biosciences, University Of kent. Canterbury. Kent.
Jeffries, P. Gianinazzi, S. Perotto, S. Turnau, K. Barea, J. M. 2003. The contribution of arbuscular mycorrhizal fungi in sustainable maintenance of plant health and soil fertility. Biology and Fertility of Soils. 37: 116.
Joner, E. and Leyval, C. (1997). Uptake of 109Cd by roots and hyphae of a Glomus mosseae/Trifolium subterraneum mycorrhiza from soil amended with high and low concentrations of cadmium. NewPhytologist, 135:353-360.
Joner, E. and Leyval, C. 2001. Time-course of heavy metal uptake in maize and clover as affected by root density and different mycorrhizal inoculation regimes. Biology and Fertility of Soils, 33(5), 351-357.
Karimian, N. 1998. Excessive consequences for the use of phosphate fertilizers. Journal of Soil and Water Sciences, Vol 12, pp. 1-14
Khan, A. G. 2006. Mycorrhizoremediation—an enhanced form of phytoremediation. Journal of Zhejiang University Science B, 7(7), 503-514.
Khan, N. Qasim, M. Ahmed, F. Khan, R. Khanzada, A. and Khan, B. 2002. Effects of sowing date on yield of maize under Agroclimatic condition of Kaghan Valley. Asian Journal of plant Science: 1(2): 140-147.
Kochaki, A.R., Tabrizi, L., and R., Ghorbani. 2008. Evaluation of the Effect of Biological Fertilizers on Growth, Function and Quality Characteristics of Hyssopus officinali. Iranian Journal of Crop Research, Vol. 6th, No. 1. Pages 137-127.
Lindsay, W. L. and Norvell, W. A. 1978. Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil science society of America journal, 42(3), 421-428.
Liu, D. Zou, J. Meng, Q. Zou, J. Jiang, w. 2009.Uptake and accumulation and oxidative stress in garlic (Allium sativum L.) under lead phytotoxicity.Ecotoxicology.18:134-143.
Luo, C. Shen, Z. Lou, S. and Li, X. 2005. Enhanced phytoextraction of Cu, Pb, Zn and Cd with EDTA and EDDS. Chemosphere. 59:1–11.
McGrath, S. P. Zhao, F. J. and Lombi, E. 2001. Plant and rhizosphere processes involved in phytoremediation of metal-contaminated soils. Plant and soil, 232(1), 207-214.
Nelson, E. O. 1982. soil pH and lime requirment. Amercian society of Agronomy, Madison, 199-224.
Olsen, S. R. Cole, C. V. watanabe . F. S.  and Dean, L. A. 1954. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. United States Department Of Agriculture; Washington.
Oskuei. P, Aliasgharzadeh. N, Shariatmadari, H and Baghban. Sh. 2008. Effect of two species of arbuscular mycorrhizal fungi on reducing cadmium toxicity in tomato plants with different levels of phosphorus. Journal of Soil Research. 23 (3): 217-228.
Perner, H. Schwarz, D. Krumbein, A. and George, E. 2011. Influence of sulfur supply, ammonium nitrate ratio, and arbuscular mycorrhizal colonization on growth and composition of Chinese chive. Scientia horticulturae, 130(3), 485-490.
Prasad, M. N. V. and Strzałka, K. 1999. Impact of heavy metals on photosynthesis. In Heavy Metal Stress in Plants (pp. 117-138). Springer Berlin Heidelberg.
Premsekhar, M. and Rajashree, V. 2009. Influence of bio-fertilizers on the growth characters, yield attributes, yield and quality of tomato. American-Eurasian Journal of Sustainable Agriculture, 3(1), 68-70.
Rajai, M., and N. Karimian. 2005. Added cadmium effect and time of bedding on chemical forms of cadmium, growth and chemical composition of spinach in two soil texture. Proceedings of Soil Conference, Environment and Sustainable Development, Karaj, College of Agriculture and Natural Resources, University of Tehran. Page 322-321.
Razileh, S. Rovera, M. Andres, J. and Correa, N. 2007. Effect of phosphorous solubilizing bacteria on the rhizobialegume symbiosis. Proceedings of the 15th International Meeting on Microbial phosphate Solubilization. Salamanca University, 16-19 July, Salamanca, Spain.
Rejali, F., Alizadeh, A., Melkotti, J. And N., Saleh Rastin. 2007. Effect of Arbuscular Mycorrhizal Synergistic Interaction on Increase, Performance, and Absorption of Mineral Ingredients in Wheat Plants under Drought Stress. Journal of Soil and Water Sciences, Volume 21, 2.
Rokni, N., 1999. Principles of Food Health, Third Edition, Publications and Printing of Tehran University. Page 1-154.
Salt, D. E. Smith, R. D. and Raskin, I. 1998. Phytoremediation. Annual Review of Plant Biology. 49: 643-668.
Sanitadi Toppi, L. and R. Gabrielli. 1999. Response to cadmium in higher plants. Environmental and experimental botany, 41(2), 105-130.
Sensoy, S. Demir, S. Turkmen, O. Erdinc, C. and Savur, O. B. 2007. Responses of some different pepper (Capsicum annuum L.) genotypes to inoculation with two different arbuscular mycorrhizal fungi. Scientia Horticulturae, 113(1), 92-95.
Shirazi, S.P., Ronaghi, A., Karimian, N., Yasrebi, J., and U., Imami. 2012. Effect of cadmium toxicity on nitrogen and phosphorus uptake and some vegetative characteristics of shoots of seven rice cultivars. Science and technology of greenhouse cultivation. 3 (9): 75-72.
Singh, H. P. and Singh, T. A. 1993. Effect of VA mycorrhizae in chickpea, Mycorrhizae. 3: 37-39.
Varvara, P. Grichko. Brendan, F. Bernard and Glick, R. 2000. Increased ability of transgenic plants expressing the bacterial enzyme ACC deaminase to accumulate Cd, Co, Cu, Ni, Pb, and Zn. Jornal of Biotechnology. 81: 45-53.
Vassilev, A. Vangronsveld, J. and Yordanov, I. 2002. Cadmium phytoextraction: Present state, biological backgrounds and research needs. Bulgarian Journal of Plant Physiology. 28: 68–95.
Weller, D. Linda, M. and Thomashow, S. 2003. Use of rhizobacteria for biocontrol. Current Opinion Biotechnology. 4: 306-311.
Wu, Q. S. and Xia, R. X. 2006. Arbuscular mycorrhizal fungi influence growth, osmotic adjustment and photosynthesis of citrus under well-watered and water stress conditions. Journal of plant physiology, 163(4), 417-425.
Wu, S. C. Cheung, K. C. Luo, Y. M. and Wong, M. H. 2006. Effects of inoculation of plant growth-promoting rhizobacteria on metal uptake by Brassica juncea. Journal of Environmental Pollution, 140: 124-135.
Yaghubzade, F. Eradatmand, D. and Yusefirad, M. 2011. Comparison of sunflower and maize plants in phytoremediation of cadmium in soils. First National Congress on Science and Technology of Agriculture, University of Zanjan. Pp: 1-4. (In Persian).
Yusefirad, M. and Karbalaei Esmaeil, M. R. 2010. Effects of mycorrhizal fungi on uptake of macronutrients and some morphological traits in sunflower cultivars (Heliantus annus L.). Second National Conference on Agriculture and Sustainable Development: Opportunities and Challenges Facing. Pp: 1-13. (In Persian).
Zhang, H. H. Tang, M. Chen, H. Zheng, C. L. and Niu, Z. C. 2010. Effect of inoculation with AM fungi on lead uptake, translocation and stress alleviation of Zea mays L. seedlings planting in soil with increasing lead concentrations. European Journal of Soil Biology, 46(5), 306-311.
Iranian Journal of Soil and Water Research
Volume 50, Issue 10
March 2020
Pages 2413-2423

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