Effect of different levels of nitrogen and nitrate ammonium ratios on yield and essential oil content of Thyme daenensis (Thymus daenensis subsp. daenensis Celak) in saline and non-saline conditions

Document Type : Research Paper


1 MSc Student, Dept. of Soil science, Faculty of Agriculture, Urmia University, Iran.

2 Associate Professor, Dept. of Soil Science, Faculty of Agriculture, Urmia University, Iran.

3 Assistant Professor, Dept. of Agronomy, Faculty of Agriculture, Urmia University, Iran.


In order to investigate the effect of different levels of nitrogen and nitrate: ammonium ratios on yield and oil percentage of Thyme daenensis in saline and non-saline conditions, a factorial experiment was conducted in a randomized complete block design (RCBD) with 30 treatments and three replicates in perlite and sand medium. Nitrogen at three levels (5, 10 and 15 mM), nitrate: ammonium ratio at five levels (100:0, 75:25, 50:50, 25:75, 0:100) and salinity at two levels (0 and 50 mM sodium chloride) were applied. Results showed that the highest fresh and dry weight, percentage and yield of crop oil were obtained at 15 mM nitrogen and 75:25 nitrates: ammonium ratio in the non-saline conditions. In the saline condition the fresh and dry weight, percentage and yield of crop oil were reduced in all treatments and the highest yield was obtained in 15 mM nitrogen and 50:50 nitrates: ammonium ratio. Generally, it was concluded that the ratio of 75:25 nitrate: ammonium in non-saline condition and the ratio of 50:50 nitrate: ammonium in saline condition were the optimum ratios at 15 mM nitrogen level for obtaining the highest yield and oil percentages.


Main Subjects

Abdelgadir, E. M., Oka, M. and Fujiyama, H. (2005). Characteristics of nitrate uptake by plants under salinity. Journal of Plant Nutrition, 28(1), 33-46.
Archangi, A., Khodambashi, M. and Mohammadkhani, A. (2012). The effect of salt stress on morphological characteristics and Na+, K+ and Ca+ ion contents in medicinal plant fenugreek (Trigonella foenum graecum L.) under hydroponic culture. Ejgcst, 3(2), 33-41. (In Farsi).
Ashraf, M. (1999). Interactive effect of salt (NaCl) and nitrogen form on growth, water relations and photosynthetic capacity of sunflower (Helianthus annum L.). Annals of Applied Biology, 135(2), 509-513.
Aziza, E. E., Al-Amier, H. and Craker, L. E. (2008). Influence of salt stress on growth and essential oil production in peppermint, pennyroyal, and apple mint. Journal of Herbs, Spices and Medicinal Plants, 14(1-2), 77-87.
Azizi, K., Amini, M., Sabouri, B. and Shabani, G. (2008). Effect of Different Levels of Nitrogen Fertilizer and Plant Density on Quantitative and Qualitative Cumin Function in Lorestan. 10th Congress of Agronomy and Plant Breeding, Tehran, Abu Aryan University Campus, Tehran University. (In Farsi).
Basirat, M. (2011). Familiarity with nutritional abnormalities of greenhouse vegetables (cucumber, tomato and pepper), Soil and Water Researches Institute, Tehran. (In Farsi).
Borgognone, D., Colla, G., Rouphael, Y., Cardarelli, M., Rea, E. and Schwarz, D. (2013). Effect of nitrogen form and nutrient solution pH on growth and mineral composition of self-grafted and grafted tomatoes. Scientia Horticulturae, 149, 61-69.
Botella, M. A., Cerda, A., Martinez, V. and Lips, S. H. (1994). Nitrate and ammonium uptake by wheat seedlings as affected by salinity and light. Journal of Plant Nutrition, 17(5), 839-850.
Bourgeais-Chaillou, P., Perez-Alfocea, F. and Guerrier, G. (1992). Comparative effects of N sources on growth and physiological responses of soyabean exposed to NaCI–stress. Journal of Experimental Botany, 43(9), 1225-1233.
Bybordi, A. (2011). Effect of different ratios of nitrate and ammonium on seed yield, oil yield, physiological attributes and fatty acid composition of canola under conditions of salt stress. Journal of Food, Agriculture and Environment, 9(3and 4), 109-112.
Cerda, A. and Martinez, V. (1988). Nitrogen fertilization under saline conditions in tomato and cucumber plants. Journal of Horticultural Science, 63(3), 451-458.
Choudhary, R. K. and Choudhary, D. N. (2005). Effect of different levels of nitrogen and phosphorus on growth, yield and quality of hybrid cabbage. Haryana Journal of Horticultural Sciences, 34(1/2), 145.
Smith, C. A., Svoboda, K. P. and Noon, M. M. (1996), September. Controlling the growth and quality of hydroponically-grown basil (Ocimum basilicum L.)(REFEREED). In International Symposium Growing Media and Plant Nutrition in Horticulture, 450, 479-486.
Darnell, R. L. and Stutte, G. W. (2001). Nitrite concentration effects on NO3-N uptake and reduction, growth, and fruit yield in strawberry. Journal of the American Society for Horticultural Science, 126(5), 560-563.
Delshad, M., Babalar, M. and Kashi, A. K. (2000). Effect of NH4/NH4+ NO3 ratio of nutrient solutions on greenhouse tomato cultivars in hydroponic systems. Iranian Journal of Agricultural Sciences, 31(3), 613-625. (In Farsi).
Dow, A. I., Horning, E. V. and Cline, T. A. (1981). Salt tolerance studies on irrigated mint. Washington State University Agricultural Research Center.
Ehsanipour, V., Zinally, H. and Razjjoo, K. H. (2012). Effect of Nitrogen Fertilizer Amounts on qualitative characteristics and grain yield in different populations of fennel. Journal of Medicinal Plants, 11 (9), 37-47. (In Farsi).
Errebi, M. and Wilcox, G. F. (1990). Plant species response to ammonium-nitrate concentration ratios. Journal of Plant Nutrition, 13(8), 1021-1029.
Estaji, A., Roosta, H. R. and Raghami, M. (2017). Comparison of vegetative traits and root yield of licorice (Glycyrrhiza glabra) influenced by different sources of nitrogen in several soilless and soil culture systems. Ejgcst, 8(2), 105-117. (In Farsi).
Feigin, A. (1985). Fertilization management of crops irrigated with saline water. Plant and Soil. 89, 285-291.
Flores, P., Navarro, J., Carvajal, M., Cerdá, A. and Martínez, V. (2003). Tomato yield and quality as affected by nitrogen source and salinity. Agronomie, 23(3), 249-256.
Golcz, A., Politycka, B. and Seidler-Lozykowska, K. (2006). Effect of nitrogen fertilization and stage of plant development on the mass and quality of sweet basil leaves (Ocimum basilicum L.). Herba Polonica.
Gulser, F. (2005). Effects of ammonium sulphate and urea on NO3− and NO2− accumulation, nutrient contents and yield criteria in spinach. Scientia Horticulturae, 106(3), 330-340.
Habibi, H., Mazaheri, D., Majnonhosseini, N., Chachichi, M. R., Tabatabayi, M. and Bigdeli, M. (2007). Evaluation of the Effect of Organic (Biological) and Nitrogenous (Urea) Sources on yield and amount of secondary metabolites of two species of wild thyme (Thymus spp.). Ph. D. dissertation, Faculty of Agriculture, Department of Agriculture. University of Tehran. (In Farsi).
Hajer, A. S., Malibari, A. A., Al-Zahrani, H. S. and Almaghrabi, O. A., (2006). Responses of three tomato cultivars to sea water salinity 1. Effect of salinity on the seedling growth. African Journal of Biotechnology, 5, 855-861.
Hasani, A. and Omidbeygi, R. (2002). Effects of water stress on some morphological, physiological and metabolic properties of basil. Agricultural Knowledge, 12 (3), 47-59. (In Farsi).
Hasanimalayeri, S., Omidbaigi, R. and Sefidkon, F. (2004). Effect of N-fertilizer and plant density on growth, development, herb yield and active substance of feverfew (Tanacetum parthenium ct. Zardband) medicinal plant. In: Proceedings of 2th International Congress on Traditional Medicin and Materia Medica. Tehran, Iran. 2, pp. 65-65. (In Farsi).
Heydari, M. and Jahantighi, H. (2012). Effect of Drought Stress and Nitrogen Fertilizer on Yield, Yield Components, Essential Oil, and Thymokinone Levels of Nigella sativa (Nigella sativa L.). Journal of Environmental Tensions in Crop Science, 5 (1), 33-40. (In Farsi).
Heydari, M., Abdulzadeh, A. and Farzaneh, F. (2011). Effect of different levels of salinity and three types of nitrogen nutrition on growth and biochemical reactions of the psyllium. Journal of Crop Sciences in Iran, 42(1), 199-207. (In Farsi).
Hoagland, D. R. and Arnon, D. S. (1950). The water culture method for growing plants without soil. California Agriculture, 374, 1-32.
Hohjo, M., Kuwata, C., Yoshikawa, K. and Ito, T. (1995). Effects of nitrogen form, nutrient concentration and Ca concentration on the growth, yield and fruit quality in NFT-tomato plants. Hydroponics and Transplant Production, 396, 145-152.
Ikeda, H. and Osawa, T. (1983). Effects of ratios of NO3:NH4 and concentrations of each N source in the nutrient solution on growth and leaf N constituents of vegetable crops and solution pH. Journal of the Japanese Society for Horticultural Science, 52, 363-380.
Jackson, G. D. and Dubbs, A. L. (1987). Spring wheat and barley response to urea fertilizer placement and nitrogen rate. Montana Agriculture, 4, 10-13.
Kafkafi, U., Valoras, N. and Letey, J. (1982). Chloride interaction with nitrate and phosphate nutrition in tomato (Lycopersicon esculentum L.). Journal of Plant Nutrition, 5(12), 1369-1385. 
Karray-Bouraoui, N., Rabhi, M., Neffati, M., Baldan, B., Ranieri, A., Marzouk, B., Lachaal A. and Smaoui, A. (2009). Salt effect on yield and composition of shoot essential oil and trichome morphology and density on leaves of Mentha pulegium. Industrial Crops and Products30(3), 338-343.
Kerepesi, I., and Galiba, G. (2000). Osmotic and salt stress-induced alteration in soluble carbohydrate content in wheat seedlings. Crop Science, 40(2), 482-487.
Khorsandi, A., Hasani, A., Sefidkan, F., Shirzad, H. and Khorssandi, A. (2010). The effect of sodium chloride salinity stress on growth, yield and essential oil composition Agastache feoniculum kunts. Iranian Journal of Medicinal Plants and Herbs Research, 26(3), 438-451. (In Farsi).
Kurvits, A. and Kirkby, E. A. (1980). The growth and mineral composition of sunflower plant, utilizing nitrate- or ammonium- N. Acta Horticultuerae, 98,139-147.
Lobit, P., Lopez-Perez, L., Cardenas-Navarro, R., Castellanos-Morales, V. C. and Ruiz-Corro, R. (2006). Effect of ammonium/nitrate ratio on growth and development of avocado plants under hydroponic conditions. Canadian Journal of Plant Science, 87, 99-103.
Malakooti, M. (2000). Balanced nutrition of wheat, the way to self-sufficiency and community health. Articles Collections, Publication of Agricultural Education. Karaj. (In Farsi).
Malakooti, M. J. and Homayi, M. (2004). Fertility of arid and semi-arid soils. Tarbiat Modares University Press. Tehran. (In Farsi).
Marschner, H. (1995). Mineral nutrition of higher plants. Academic Press:  London.
Mengel, K. and Kirkby, E. A. (2001). Principles of plant Nutrition. Kluwer Academic pub London UK.
Moniruzzaman, M., Rahman, S. M. L., Kibria, M. G., Rahman, M. A. and Hossain, M. M. (2007). Effect of boron and nitrogen on yield and hollowstem of broccoli. Journal of Soil Nat, 1(3): 24-29.
Mozaffarian, V. (1997). Iran Plants Dictionary. Farhang Musavar Publishing. Tehran: Iran. (In Farsi).
Munns, R., James, R. A. and Läuchli, A. (2006). Approaches to increasing the salt tolerance of wheat and other cereals. Journal of Experimental Botany, 57(5), 1025-1043.
Najafi, N., Parsazadeh, M., Tabatabaei, S. J. and Ostan, S. (2010a). Effect of pH and nitrate/ ammonium ratio of nutrient solution on growth and yield characteristics of spinach. Iranian Journal of Soil and Water Research, 41(2): 273-282. (In Farsi).
Najafi, N., Parsazadeh, M., Tabatabaei, S. J. and Ostan, S. (2010b). Effect of nitrogen form and pH of nutrient solution on the uptake of Fe, Zn, Cu and Mn by spinach plant in hydroponic culture. Iranian Journal of Soil and Water Research, 41(2): 283-295. (In Farsi).
Neffati, M. and Marzouk, B. (2008). Changes in essential oil and fatty acid composition in coriander (Coriandrum sativum L.) leaves under saline conditions. Industrial Crops and Products, 28(2), 137-142.
Niakan, M., Khavrnejad, R. A., and Rezaei, M. B. (2004). Effect of different ratios of nitrogen: phosphorus: potassium on fresh weight of leaf, dry weight, leaf area and essential oil content in Mentha piperita L. Journal of Research in Iranian Medicinal and Aromatic Plants, 20(2), 131-148. (In Farsi).
Nielsen, D. C., and Halvorson, A. D. (1991). Nitrogen fertility influence on water stress and yield of winter wheat. Agronomy Journal, 83(6), 1065-1070.
Nikavar, B., Mojab, F. and Dolatabadi, R. (2004). Investigation of Thyme Components of Essential Flowers of Thyme. Medicinal Plants, 1(13), 45-49. (In Farsi).
Ozturk, A. H. M. E. T., Unlukara, A., Ipek, A. R. İ. F. and Gurbuz, B. İ. L. A. L. (2004). Effects of salt stress and water deficit on plant growth and essential oil content of lemon balm (Melissa officinalis L.). Pak. Journal of Bot, 36(4), 787-792.
Petersen, K.K., Willumsen, J. and Kaack, K. (1998). Composition and taste of tomatoes as affected by increased salinity and different salinity sources. The Journal of Horticultural Science and Biotechnology, 73(2), 205-215.
Rahayu, Y. S., Walch-Liu, P., Neumann, G., Römheld, V., von Wirén, N. and Bangerth, F. (2005). Root-derived cytokinins as long-distance signals for NO3−-induced stimulation of leaf growth. Journal of Experimental Botany, 56(414), 1143-1152.
Rahmani, N., Taherkhani, T. and Daneshian, J. (2009). Effects of water deficit stress and application of nitrogen on yield physiological indices of Calendula (Calendula officinalis L.). Journal of New Finding in Agriculture, 3(4), 355-365. (In Farsi).
Saeedigharaghani, H., Yazdanibeyki, R., Saeedigargaghani, N. and Soodaedzadeh, H. (2014). Effect of Sources and Nitrogen Different Quantities on Quantitative and Qualitative Properties of Parsley (Petroselinum crispum Mill) in Jiroft Region. Iranian Journal of Crop Research, 12(2), 316-327. (In Farsi).
Safarzadeh-Shirazi, S., Ronaghi, A. M., Gholami, A. S. and Zahedifar, M. (2010).The Influence of salinity and nitrogen on tomato fruit quality and micronutrients concentration in hydroponic culture. Ejgcst, 1 (3), 11-22. (In Farsi).
Safikhani, F. (2007). The effect of stress drought on the quantitative and qualitative performance of the medicinal plant Badrashbu Under farm conditions. Ph. D. dissertation on agriculture, University of Ramin, Ahwaz. (In Farsi).
Salami, M., Safarinezhad, A. and Hamidi, H. (2006). Effect of Salinity on Morphological Characteristics of Cumin and Valerian. Research and Construction in Natural Resources, 72, 77-83. (In Farsi).
Sharafzadeh, S., Khosh-Khui, M., Javidnia, K., Alizadeh, O. and Ordookhani, K. (2010). Identification and comparison of essential oil components in leaf and stem of garden thyme grown under greenhouse conditions. Advances in Environmental Biology, 4(3), 520-523. (In Farsi).
Sharifi, P. and Amiryousefi, M. (2017). Effect of Nitrogen and Azotobacter on Yield and Yield Components of Bright Wheat Cultivars. Journal of Agricultural Knowledge and Sustainable Production, 27(2), 125-144. (In Farsi).
Shaviv, A., Hazan, O., Neumann, P. and Hagin, J. (1990). Increasing salt tolerance of wheat by mixed ammonium nitrate nutrition. Journal of Plant Nutrition, 13(10), 1227-1239.
Shekofte, H., Salari, N. and Abdi, S. (2015). Effect of different ratios of nitrate to ammonium and superabsorbent polymer on the yield of the medicinal plant (Anethum graveolens L). Two Chapters on Plant Production Technology, 7(2), 55-68. (In Farsi).
Shima, K. (1999). Role of Ammonium and Nitrate as Nitrogen Source on Salt Tolerance in Nerium oleander L. Journal of the Japanese Society of Revegetation Technology, 23(4), 237-248.
Silberbush, M. and Lips, S. H. (1991). Potassium, nitrogen, ammonium/nitrate ratio, and sodium chloride effects on wheat growth: I. Shoot and root growth and mineral composition. Journal of Plant Nutrition, 14(7), 751-764.
Sobhani, G., Golchin, A. and Shekari, F. (2014). Effects of different levels of nitrogen and induced-NaCl stress on yield and growth indices of tomato. Ejgcst, 5(3), 49-63. (In Farsi).
Tabatabaei, S. J., Fatemi, L. S. and Fallahi, E. (2006). Effect of ammonium: nitrate ratio on yield, calcium concentration, and photosynthesis rate in strawberry. Journal of Plant Nutrition, 29(7), 1273-1285. (In Farsi).
Taheri, M., Talaei, A., Babalar, M., Taghavi, T. and Ebadi, A. (2009). Effect of nitrogen source, planting time on photosynthesis rate, growth and macro in two varieties of olive sapling. Journal of Horticultural Science and Technology, 10 (1), 29-42. (In Farsi).
Talayi, A. (1998). Physiology of Fruit Trees in Temperate Areas. (Compilation of Michels Favost). Tehran University Press. (In Farsi).
Tandisebana, S., Astarayi, A. and Emami, H. (2016). Effect of soil salinity, light and nitrogen fertilizer on yield and biochemical characteristics of mustard shoots. Iranian Crop Research, 14(4), 575-586. (In Farsi).
Torbatinezhad, N. M., chayichi, M. R. and Sharifi, S. (2002). Effect of Nitrogen Levels on Yield and Yield Components of Three Forage Sorghum Cultivars in Gorgan Region. Journal of Agricultural Science and Natural Resources. 9(2), 205-220. (In Farsi).
Traore, A. and Maranville, J. W. (1999). Effect of nitrate/ammonium ratio on biomass production, nitrogen accumulation, and use efficiency in sorghums of different origin. Journal of Plant Nutrition, 22(4-5), 813-825.
Tyson, R. V., Simonne, E. H., Davis, M., Lamb, E. M., White, J. M. and Treadwell, D. D. (2007). Effect of nutrient solution, nitrate-nitrogen concentration, and pH on nitrification rate in perlite medium. Journal of Plant Nutrition, 30(6): 901- 913.
Veen, B. W. and Kleinedorst, A. (1985). Nitrate accumulation and osmotic regulation in Italian ryegrass (lolium multiflorum Lam). Journal of Experimental Botany, 36, 211-218.
Wang, L. J., Liu Y. J., Ma, K., Wang, J. Z. and Liu, X. N. (1998). Effect of NaCl treatment on free radical metabolism of fig (Ficus cartica L. Calli). Advanced Horticulture, 2, 235-241.
Woodson, W. R. and Boodley, J. W. (1982). Effects of nitrogen form and potassium concentration on growth, flowering and nitrogen utilization of greenhouse roses. Journal American Society for Horticultural Science, 107, 275-278.
Zahedifar, M., Ronaghi, A. M., Moosavi, S. A. A. and Safarzadeh-Shirazi, S. (2010). The effect of salinity and nitrogen on nutrient distribution, citric acid and vitamin C in hydroponics. Ejgcst, 1(3), 23-30. (In Farsi).
Zaremehrjardi, M., Nabati, J., Masoumi, A., Bagheri, A. and Kafi, M. (2011). Investigation of root and shoot salinity tolerance of eleven genotypes tolerant and sensitive to drought stress in hydroponic conditions. Research of Iranian Beans, 2(2), 83-96. (In Farsi).