Comparison the effect of ZnSO4 and Zn- lysine aminio chelate foliar application on growth indices of Behbahan onion

Document Type : Research Paper


1 Khuzestan Agricultural and Natural Resources Research and Education Center , AREEO, Ahwaz, Iran

2 Department of Soil Science, Isfahan University of Technology, Isfahan, Iran

3 Isfahan University of Technology

4 Seed and Plant Improvement Department, Khuzestan Agricultural and Natural Resources Research and Education Center , AREEO, Ahwaz, Iran


This field experiment was conducted to investigate the effect of foliar application of zinc (Zn) in the form of ZnSO4 and Zn-Lys amino chelate on growth indices and yield of Behbahan onion at the Agriculture Research Station of Behbahan, Iran. The experiment was conducted in a randomized complete block design with four treatments and three replications. Foliar application treatments were: ZnSO4, Zn chelated with lysine (Zn-Lys), and free amino acid of lysine (Lys). A treatment with no foliar application of Zn and amino acid was considered as the control. Foliar application was performed at two stages, once at the early rapid vegetative growth stage and the other at the early bulbing. The results of growth analysis indicated that at all fertilizer treatments, the slow growth period lasted to 125 days after seed germination followed by the rapid growth period. The maximum leaf area index (LAI) (4.19), bulb growth rate (30.49 g m-2 day-1), crop growth rate (39.66 g m-2 day-1) and total and marketable yield of bulb (63.07 and 60.54 t ha-1, respectively) was obtained at the Zn-Lys amino chelate. Among different treatments, the pattern of dry matter accumulation showed that Zn-Lys amino chelate resulted significant increase of plant dry mass (62% over the control treatment), and this trend continued until the end of the growing season. A positive and significant correlation (P<0.01) was found between relative growth rate and net assimilation rate.


Main Subjects

Alcazar, R., Altabella, T., Marco, F., Bortolotti, C., Reymond, M., Koncz, C., Carrasco, P. and Tiburcio, A. F. (2010). Polyamines: molecules with regulatory functions in plant abiotic stress tolerance. Planta 231, 1237–1249.
Alloway, B. J. (2008). Zinc in soils and crop nutrition (2th ed.).IZA and IFA Brussels, Belgium and Paris, France.
Anonymous. (2016). Agricultural statistics. Horticultural and agronomic crops. Ministry of Jihad-e- Agriculture. Tehran. Iran. Pp: 6-100. (In Farsi).
Arshadi, M. J., Khazaie, H. R. and Kafii, M. (2014). The effect of using the chlorophyll meter fertilizer       topdressing on yield, yield components and growth indices potatoes. Mashhad Ferdowsi University. Iranian Journal of Field Crops Research,11(4), 573-582. (In Farsi).
Bouyoucos, C. J. (1962). Hydrometer method improved for making particle size analysis of soil. Agron. J.54, 464-465.
Bremner, J. M. and Mulvancy, C. S. (1982). Nitrogen-Total. In: page, A. L., et al. (Eds.), Methods of Soil Analysis. Part 2. Agron. Monogr. 9. ASA and SSSA, Madison, WI, pp. 595–624.
Brewster, J. L. (1990). Physiology of crop growth and bulbing In: J. L. Brewster and H. D. Rabinowwitch (Eds). Onions and Allid Crops. Volume l. Botany. Physiology and Genetic. CRC, Press. Boca Raton. Pp. 53-58.
Brewster, J. and Rabiniwitch, H. D. (1997). Onions. Lecture notes on selected topics in vegetable production. (27th ed.). International Course on Vegetable Production. lAC, Wageningen, Netherlands.
Brewster, J. L. and Suterland, R. A. (1993). The rapid determination in controlled environments of parameters for predicting seedling growth rate in natural conditions. Ann. Appl. Biol. 122, 123-133.
Bybordi, A. and Melekoti, M. J. (1998). The necessary of optimum application of  fertilizer for increasing quantitative  and qualitative of yield and decreasing nitrate concentration in onion bulbs. Agricultural education publication. Karaj. Iran. 20 p. (In Farsi).
Corzo-Martinez, M., Corzo, N. and Villamiel. M. (2007). Biological properties of onionsand garlic. Trends Food Sci Tech. 18, 609–625.
Currah, L. (2002). Onion in the tropics: cultivars and country reports. PP. 379-407. in: Rabinowich, H. D. and L. Currah (eds). Allium Crop Science: Recent Advanceds. CABI pulishing. U. K.
Darabi, A., Kashi, A., Babalar, M. and Khodadadi, M. (2008). Changes of simple non-structural carbohydrates during bulb formations and growth analysis of four onion cu;tivars in behbahan. Water, Soil & Plant in Agriculture.  8. 1(B), 37-50.
Darabi, A. (2009). Study of Bulbing physiology in important local population of Iranian onion in behbahan and karaj provinces. Ph. D. thesis. Tehran University. Tehran. Iran 162 p. (In Farsi).
Darabi, A., Kashi, A., Babalar, M. and Khodadadi, M. (2009). Growth analysis and evaluation of change in simple non-structural carbohydrates during bulb formation of onion cultivars in Karaj climatic condition. Ferdowsi University of Mashhad, 23(1), 36-45. (In Farsi).
Darabi, A., Kashi, A., Babalar, M. and Khodadadi, M. (2010). Determination the threshold of photoperiodism and cumulative thermal time related to bulbing and growth analysis of onion cultivars. Iranian Journal of Agricultural Sciences, 40(4), 23-33. (In Farsi).
Darabi, A. and Khodadadi, M. (2012). Determination of bulbing time and growth analysis of onion landraces in climatic conditions of Karaj. Scientific Journal of Agriculture, 34(2-A), 1-14. (In Farsi).
Darabi, A. (2016). Physiology and production of onion (1th ed.). Agricultural Research, Education and Extension Organization. Tehran. Iran (In Farsi). 276 p.
El-Bassiouny, H. M. S., Mostafa, H. A., El-Khawas, S. A., Hassanein, R. A., Khalil, S. I. and Abd El-Monem, A. A. (2008). Physiological responses of wheat plant to foliar treatments with arginine or putrescine. Australian Journal of Basic and Applied Sciences, 2, 1390-1403.
Gamelli, H. H. (2000). The effect of some foliar fertilizers application on growth, bulbplant biomass, quality and storage ability of Giza 20 onion cultivar (Allium cepaL.). Ann. Agric. Sci. Moshtohor, 38, 1727–1737.
Gardner, F. P., Pearce, R. B. and Mitchel, R. L. (1985). Physiology of Crop Plant. Iowa State University Press. USA. pp. 186-208.
Ghasemi, S., Khoshgoftarmanesh, A. H., Afyuni, M. and Hadadzadeh, H. (2013). The effectiveness of foliar applications of synthesized zinc-amino acid chelates in comparison with zinc sulfate to increase yield and grain nutritional quality of wheat. Eur. J. Agr. 45, 68-74.
Griffiths, G., Trueman, L., Crowther, T., Thomas, B. and Smith, B. (2002). Onions-a globalbenefit to health. Phytother. Res. 16, 603–615.
Haluschak, P. (2006). Laboratory methods of soil analysis. Canada-Manitoba soil survey, 3-133.
Hunt, R. (1982). Plant growth curves. The functional approach to plant growth analysis. Enwards Arnold Publlication, London, UK. pp. 248.
Iortsuun, D. N. and Khan, A. A. (1989). The pattern of dry matter distribution during development in onion. J. Agron. Crop Sci.  162, 127-134.
Kahane, R., Vaillle-Guerin, E., Boukema, I., Tzanoudakis, D., Bellamy, C., Chamaux, C. and Kik, C. (2001). Changes in non- structural carbohydrate composition during bulbing in sweet and high-solid onions in field experiments. Env. Exp. Bot. 45, 72-83.
Khoshgoftarmanesh, A. H., Schulin, R., Chaney, R. L., Daneshbakhsh, B. and Afyuni, M. (2010). Micronutrient-efficient genotypes for crop yield and nutritional quality in sustainable agriculture. A review. Agron. Sustain. Dev. 30, 83–107.
Koocheki, A., RashedMohassel, M. H., Nasiri, M. and SadarAdadi, R. (1995). Physiological of plant growth and development. Emam Reza University. Mashhad. Iran. 404 p. (In Farsi).
Koocheki, A. and Sarmadnia, G. (1999). Physiology of crop plants. Jahad- e- Daneshgahi publication. Mashhad University. Iran. 400 p. (In Farsi).
Lancaster, J. E., Trigs, C. M., De Ruiter J. M. and Gander. P. W. (1996). Bulbing in onions: photoperiod and temperature requirements and prediction of bulb size and maturity. Ann. Bot. 78, 423-430.
Lindsay, W. L. and Norvell, W. A. (1978). Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Sci. Soc. Am. J. 42, 421–428.
Lonnerdal, B. (2000). Dietary factors influencing zinc absorption. J. Plant Nutr. 130, 1378–1383.
Marschner, H. (1995). Mineral nutrition of higher plants. Academic Press, London, U. K.
Matsui, S., Maedia, M., Shirial, M. and Kamur, Y. (1994). Growth analysis of onion (Allium cepa L.) plants treated with (s) - (+) – abscicic acid. Research bulletin of the Faculty of Agriculture Gifu University 59, 57-62.
Mishra, H. P., Singh, K. P. and Yadav, J. P. (1990). Influence of Zn, Fe, B and Mn and their uptake on onion grown in calcareous soil. Haryana J. Hort. Sci., 19, 153-59.
Mohammadi, P. and Khoshgoftarmanesh, A. H. (2014). The effectiveness of syntheticzinc(Zn)-amino chelates in supplying Zn and alleviating salt-induced damageson hydroponically grown lettuce. Sci. Hortic.-Amsterdam 172, 117–123.
Mortvedt, J. (2014). Efficient Fertilizer Use-Micronutrients, Available 10 October 2014 from http : // cyber. collegeshawinigan. qc. ca/ cpedneault/ controlequalite/ EFU% 20 manual analyse % 20 des % 20 sols /micronutrients. pdf.
Nasreen, S., Imamul, S. M. and AltabHossain, M. (2003). Sulphur effects on growth responses and yield of onion. Asi. J. of Plant Sci.  897-902.
Olsen, S. R. and Sommers, L. E. (1982). Phosphorus. In: Page, A. L., et al. (Eds.), Methods of Soil Analysis, Part 2. , 2nd edn. ASA and ASSA, Madison WI, pp. 403–430, Agron Monogr 9.
Rao, N. K. S. (1988). Physiological analysis of growth and yield in onion (Allium cepa- L.) Indan J. Agr. Sci. 58(6), 489-491.
Rastegar, J., Khodadadi, M. and Mousipoor Gorgi, A. (2006). Investigation on growth pattern of Iranian onion cultivars and landraces based on Physiological indices. Khorasan research center (Mashhad). Iran. 37 p. (In Farsi).
Sedighi Dehkordy, F. and Alemzadeh Ansari, N. (2001). The determination of growth curve for three tomato cultivars in spring production in Ahvaz condition Climate. Journal Shahid Chamran University. Ahvaz. Iran. 95 p. (In Farsi).
Sidlauskas, G. and Bernotas, S. (2003). Some factors affecting seed yield of spring oilseed rape (Brassica napusL.). Agron. Res. 1(2), 229-243.
Singh, J. and Dankhar, B. S. (1991). Effect of nitrogen, potash and zinc on storage loss of onion bulb. Vegetable science. 18, 16-23.
Singh, D. P. and Tiwari, R. S. (1995). Effect of micronutrients on growth and yield of onion variety pusa Red. Recent Horticulture. 2(2), 70-77.
Tei, F., scaife, A. and Aikman, D. P. (1996). Growth of lettuce, onion and red beet. Growth analysis, light interception and radiation use efficiency. Ann. Bot. 78, 633-644.
Tekalign, T. and Hammes, P. S. (2005). Growth and productivity of potato as influenced by cultivar and reproductive growth. II. Growth analysis, tuber yield and quality. Sci. Hort. 105, 29-44.
Walkley, A. and Black, I. A. (1934). An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Science. 37, 29-38.