Response of Olive (Olea europaea L.) self-rooted cuttings to joint salinity and soil compaction stresses in soil hypoxia conditions

Document Type : Research Paper


1 University of Zanjan

2 Professor of Soil Science Department, Faculty of Agriculture, University of Zanjan, Zanjan, IRAN.

3 Assistant Professor of Soil and Water Research Department, Zanjan Agricultural and Natural Resources Research and Education Center, Zanjan, IRAN.

4 Research Staff of Seed and Plant Improvement Institute, Zanjan Agricultural and Natural Resources Research and Education Center, Zanjan, Iran

5 Assistant Professor of Soil Science Department, University of Tabriz, Tabriz, IRAN.


Responses of Olive self-rooted cuttings to irrigation water salinity and soil compaction in moisture of 0.9 saturation were studied. A loamy sand soil passed through a 4.75mm sieve, poured into PVC cylinders and compacted to achieve the desired compaction levels (bulk density of 1.3, 1.5 and 1.7 g cm-3). Then rooted olive cuttings were transplanted into the cylinders and were irrigated by waters with different salinities of 2, 5, 8 and 12 dS‌‌ m-1 for five months. Instantaneous water use efficiency, electrolyte leakage, SPAD index and number of leaves to salinity of 8, and parameters of photosynthesis, stomatal conductance, transpiration rate, temperature difference between leaf and environment to salinity of 5 dS m-1 had no statistically meaningfull differences. Soil hypoxia decreases salinity tolerance mechanisms. With respect to olive plant growth, the bulk density of 1.3 g cm-3 was determined the optimum level of soil compaction for a loamy sand soil.


Aparicio, C., Urrestarazu, M. and Cordovilla, M. D. (2014) Comparative physiological analysis of salinity effects in six olive genotypes. HortScience 49(7), 901-904.
Askary, M., Maghsoudi Moud, A. A. and Saffari, V. R. (2013) Investigation of Some Physiological Characteristics and Grain Yield of Corn) Zea mays L.) Hybrids under Salinity Stress. Journal of Crop Production and Processing, 3(9), 93-104. (In Farsi)
Azizi, S., Tabari, M. and Striker, G. G. (2017) Growth, physiology, and leaf ion concentration responses to long-term flooding with fresh or saline water of Populus euphratica. South African Journal of Botany, 108, 229-236.
Azizian, A. and Sepaskhah, A. R. (2014) Maize response to water, salinity and nitrogen levels: physiological growth parameters and gas exchange. International Journal of Plant Production, 8(1), 131-162.
Azzarello, E., Muganai, S., Pandolfi, C., Masi, E. and Marone, E. (2009) Comparing image (fractal analysis) and electrochemical (impedance spectroscopy and electrolyte leakage) techniques for the assessment of the freezing tolerance in olive. Trees 23, 159– 67.
Ben Ahmed, C., Ben Rouina, B. and Boukhris, M. (2007) Effects of water deficit on olive trees cv. Chemlali under field conditions in arid region in Tunisia. Scientia Horticulturae, 113, 267–277.
Ben Ahmed, C., Ben Rouina, B. and Boukhris, M. (2008) Changes in water relations, photosynthetic activity and proline accumulation in one-year-old olive trees (Olea europaea L. cv. Chemlali) in response to NaCl salinity. Acta Physiologiae Plantarum, 30,553-560.
Ben Rouina, B., Trigui, A., d’Andria, R., Boukhris, M. and Chaïeb, M. (2007) Effects of water stress and soil type on photosynthesis, leaf water potential and yield of olive trees (Olea europaea L. cv Chemlali Sfax). Australian Journal of Experimental Agriculture, 47(12), 1484-1490.
Bhattarai, S. P., Pendergast, L. and Midmore, D. J. (2006) Root aeration improves yield and water use efficiency of tomato in heavy clay and saline soils. Scientia Horticulturae, 108, 278-288.
Bolaños, J. A. and Longstreth, D. J. (1984) Salinity effects on water potential components and bulk elastic modulus of alternanthera philoxeroides (Mart.) Griseb. Plant Physiology, 75, 281-284.
Campos, P. S., Quartin, V., Ramalho, J. C. and Nunes, M. A. (2003) Electrolyte leakage and lipid degradation account for cold sensitivity in leaves of Coffea sp. Plants. Journal of Plant Physiology, 160, 283-292.
Chartzoulakis, K. (2005) Salinity and olive: growth, salt tolerance, photosynthesis and yield. Agricultural Water Management, 78, 108-121.
Chartzoulakis, K., Loupassaki, M., Bertaki, M. and Androulakis, I. (2002) Effects of NaCl salinity on growth, ion content and CO2 assimilation rate of six olive cultivars. Scientia Horticulturae, 96, 235–247.
Chavez, M. M., Flexas. J. and Pinheiro, C. (2009) Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell, review. Annals of Botany, 103, 551-560.
Cimato, A., Castelli, S., Tattini, M. and Traversi, M. L. (2010) Anecophysiological analysis of salinity tolerance in olive. Environmental and Experimental Botany, 68, 214-221.
Colmer, T. D. and Voesenek, L. A. J. C. (2009) Flooding tolerance: suites of plant traits in variable environments. Functional Plant Biology, 36, 665-681.
Dag, A., Tugendhaft, Y., Yogev, U. and Shatzkin, N. (2008) Commercial cultivation of olive (Olea europaea L.) with saline water under extreme desert conditions. Acta Horticulturae, 791, 279-284.
Devitt, D., Jarrell, W. M. and Steven, K.L. (1981) Sodium–potassium ratios in soil solution and plant response under saline conditions. Soil Science Society of America Journal, 34, 80–86.
Duarte, H. H. F. and Souza, E. R. (2016) Soil water potentials and capsicum annuum L. under salinity. Revista Brasileira de Ciência do Solo, 40, 1-12.
Ghoily Kylaneh, M., Tabatabaie, S. J. and Bolandnazar, S. (2014) Effects of Light Intensity and NaCl Salinity on Yield and Uptake of Some Elements in French Tarragon (Artemisia dracunculus L.). Water and Soil Science, 24(2), 147-159. (In Farsi)
Giorio, P., Soventino, G. and d’Andria, R. (1999) Stomatal behavior, leaf water status and photosynthetic response in field—grown olive trees under water deficit. Agricultural Water Management, 42, 95-104.
Gomez, G. A., Singer, M. J., Powers, R. F. and Horwath, W. R. (2002) Soil compaction effects on water status of ponderosa pine assessed through 13C/12C composition. Tree Physiology, 22, 459-467.
González, A., Tezara, W., Rengifo, E. and Herrera, A. (2012) Ecophysiological responses to drought and salinity in the cosmopolitan invader Nicotiana glauca. Brazilian Journal of Plant Physiology, 24(3), 213-222.
Grattan, S. R. (2002) Irrigation Water Salinity and crop production. Retrieved March 7, 2017, Agriculture and Natural Resources Department. University of California. USA. Publication No. 8066. From http://
Gucci, R., Lombardini, L. and Tattini, M. (1997) Analysis of leaf water relations in leaves of two olive (Olea europaea) cultivars differing in tolerance to salinity. Tree Physiology, 17, 13–21.
Jackson, W. A. and Volk, R. J. (1997) Role of potassium in photosynthesis and respiration. In Kilmer, V. J., Younts, S. E. and Brady, N. C. (Eds), The role of potassium in agriculture. (pp. 109–188). American Society of Agronomy.
Kchaou, H., Larbi, A., Gargouri, K., Chaieb, M., Morales, F. and Msallem, M. (2010) Assessment of tolerance to NaCl salinity of five olive cultivars based on growth characteristics and Na+ and Cl exclusion mechanisms. Scientia Horticulturae, 124, 306-315.
Kaya, C., Higgs, D. and Kirnak, H. (2001) The effects of high salinity (NaCl) and supplementary phosphorus and potassium on physiology and nutrition development of spinach. Bulgarian Journal of Plant Physiology, 27, 47-59.
Kaya, C., Kirnak, H., Higgs, D. and Satali, K. (2002) Supplementary calcium enhances plant growth and fruit yield in strawberry cultivars grown at high (NaCl) salinity. Scientia Horticulturae, 93, 65-74.
Ling, Q., Huang, W. and Jarvis, P. (2011) Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana. Photosynthesis Research, 107(2), 209-214.
Liu, S., Liu, J., Cao, J., Bai, C. and Shi, R. (2006). Stomatal distribution and character analysis of leaf epidermis of jujube under drought stress. Journal of Anhui Agricultural Sciences, 34, 1315–1318.
Loreto, F., Centritto, M. and Chartzoulakis, K. (2003). Photosynthetic limitations in olive cultivars with different sensitivity to salt stress. Plant Cell and Environment, 26, 595–601.
Lutts, S., Kinet, J. M. and Bouharmont, J. (1996) NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Annals of Botany, 78, 389-398.
Meng, L., Li, L., Chen, W., Xu, Z. and Liu, L. (1999) Effect of water stress on stomatal density, length, width and net photosynthetic rate in rice leaves. Journal of Shenyang Agricultural University, 30, 477-480.
Moreno, F., Conejero, W., Martin-palomo, M. J., Giron, I. E. and Torecillas, A. (2006) Maximum daily trunk shrinkage reference values for irrigation scheduling in olive trees. Agricultural Water Management, 84, 290–294.
Motesharezadeh, B., Navabzadeh, M. and Liyaghat, A. M. (2016) Modeling phytoremediation of cadmium contaminated soil with sunflower (Helianthus annus) under salinity stress. International Journal of Environmental Research, 10(1), 109-118.
Mozaffari, V., Asadollahi, Z., Tajabadi Pour, A. and Akhgar, A. R. (2015) Effects of Salinity and Manganese on Physiological and Echophysiological Characteristics of Pistachio Seedlings (in Sand Culture). Iranian Journal of Soil and Water Research, 44(1), 81-94. (In Farsi)
Munns, R. (2002) Comparative physiology of salt and water stress, Plant Cell Environment, 20, 239–250.
Najafi, N. and Sarhangzadeh, E. (2014) Effects of soil alinization and waterlogging on the concentrations of some macronutrients and sodium in corn shoot. Water and Soil Science, 24(3), 259-275. (In Farsi)
Olyaii, F., Baninasab, B. and Ghobadi, S. (2015) Effect of salinity on gas exchange parameters in four olive cultivars. Journal of Plant Process and Function, 4(12), 51-59. (In Farsi)
Ouzounidou, G., Giannakoula, A., Ilias, I. and Zamanidis, P. (2016) Alleviation of drought and salinity stresses on growth, physiology, biochemistry and quality of two Cucumis sativus L. cultivars by Si application. Brazilian Journal of Botany, 39(2), 531-539.
Pallas, Jr. J. E., Michel, B. E. and Harris, D. G. (1967) Photosynthesis, transpiration, leaf temperature, and stomatal activity of cotton plants under varying water potentials. Plant Physiology, 42, 76-88.
Pezeshki, S. R. (2001) Wetland plant responses to soil flooding. Environmental and Experimental Botany, 46, 299–312.
Qiu, D. L., Lin, P. and Guo, S. Z. (2007) Effects of salinity on leaf characteristics and CO2/H2O exchange of Kandelia candel (L.) Druce seedlings. Journal of Forest Science, 53(1), 13-19.
Rivelli, A. R., Lovelli, S. and Perniola, M. (2002) Effects of salinity on gas exchange, water relations and growth of sunflower (Helianthus annuus). Functional Plant Biology, 29, 1405-1415.
Rolny, N., Costa, L., Carrión, C. and Guiamet, J. J. (2011) Is the electrolyte leakage assay an unequivocal test of membrane deterioration during leaf senescence? Plant Physiology and Biochemistry, 49, 1220-1227.
Rossi, L., Francini, A., Minnocci, A. and Sebastiani, L. (2015) Salt stress modifies apoplastic barriers in olive (Olea europaea L.): a comparison between a salt-tolerant and a salt-sensitive cultivar. Scientia Horticulturae, 192: 38-46.
Rush, D. W. and Epstein, E. (1978) Genotypic response to salinity difference between salt-sensitive and salt tolerance genotypes of tomato. Plant Physiology. 57, 162–166.
Tabatabaei, S. J. (2006) Effects of salinity and N on the growth, photosynthesis and N status of olive (Olea europaea L.) trees. Scientia Horticulturae, 108, 432-438.
Tattini, M., Gucci, R., Coradeschi, M. A., Ponzio, C. and Everard, J. D. (1995) Growth, gas exchange and ion content in Olea europaea plants during salinity stress and subsequent relief. Physiologia Plantarum, 95, 203–210.
Xu, Z. and Zhou, G. (2008) Responses of leaf Stomatal density to water status and its relationship with photosynthesis in a grass. Journal of Experimental Botany, 59(12), 3317-3325.
Zarehaghghi, D., Neyshabouri, M. R., Gorji, M., Monirifar, H. and Shorafa, M. (2012) Determination of Non-Limiting Water Range for Seedling Growth of Pistachio at Two Levels of Soil Compaction. Water and Soil Science, 22(3), 59-71. (In Farsi)
Zhang, Y. P., Wang, Z. M., Wu, Y. C. and Zhang, X. (2006) Stomatal characteristics of different green organs in wheat under different irrigation regimes. Acta Agronomica Sinica, 32, 70-75.