Evaluation of Shear Strength of Soil Stabilized by Microbiological Method

Document Type : Research Paper

Authors

University of Shiraz

Abstract

Biogrout is a new method in soil improvement on microbial induced calcium carbonate precipitation (MICP). In this study was utilized Sporosarcina pasteurii bacteria, an aerobic bacterium which exists pervasive in natural soil deposits, in order to control soil erosion. The undrained shear strength of the soil was measured using an in situ miniature van shear apparatus. The evaluation of shear strength are done for three types of sandy soil (Siliceous and carbonate), in four OD= 1, 1.5, 2, 2.5 levels, at different the curing time of 3, 7, 14, 20, 28 days, and, the re- injection of the bacteria solution and cementation in the interval of 6 days. Also, the influence of environmental conditions on the performance of the bacteria and the amount of shear strength are investigated. The results show that the shear strength were improved with the time. The highest shear strength was obtained in the carbonate sand and fine silica sand equals to 0.64 and 0.39 kg/cm2 in the periods of 28 days, respectively. The optimal balance between nutrients and the number of bacteria was observed at OD=1.5. Also, the results showed that the re- injection of the bacteria solution and cementation was more effective than once rejection in the shear strength, especially in the silica sand equals to 55% in a curing period of 28 days.

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Achal, V., Mukherjee, A., Basu, P. C., and Reddy, M. S. (2009). Lactose mother liquor as an alternative nutrient source for microbial concrete production by Sporosarcina Pasteurii. Journal of Industrial Microbiology and Biotechnology, 36, 433-438.
Achal, V., Mukherjee, A., and Reddy, M. S. (2010). Microbial concrete: A way to enhance the durability of building structures. Journal of Materials in Civil Engineering (ASCE), 1943-5533.
Ahmadi, H., Ekhtessasi, M.R., Feiznia, S., and Ghanei Bafghi, M.J. (2002). Control Methods of Wind Erosion for Railroads Protection (Case Study: Bafgh Region). Iranian Journal of Natural Resources, 55(3), 327-342. (In Farsi)
Al Qabany, A. (2011). Microbial Carbonate Precipitation in Soils. Ph. D, Dissertation, University of Cambridge, UK.
Amin, M. (2014). Effect of biogrouting in reduction erosion rate of sand. MS Thesis, Shiraz University, Shiraz, Iran. (In Farsi)
Bang, S.S., Leibrock, C., Smith, B., Pinkelman, R.J., Frutiger, S., Nehl, L.M., Comes, B.L., Coleman, D., and Bang, S. (2009). Geotechnical values of microbial calcite in dust suppression. Proc. of NSF Engineering Research and Innovation Conference (CD-ROM), Honolulu, HI.
Bang, S., Min, S.H., and Bang, S.S. (2011). Application of Microbiologically Induced Soil Stabilization Technique for Dust Suppression. International Journal of Geo-Engineering, 3(2), 27-37.
Benini, S., Rypniewski, W.R., Wilson, K.S., Miletti, S., Ciurli, S., and Mangani, S. (1999). A new proposal for urease mechanism based on the crystal structures of the native and inhibited enzyme from Bacillus pasteurii: why urea hydrolysis costs two nickels. Journal of Structure, 7(2), 205–216.
Bergado, D.T., Long, P. V., Balasubramaniam, A.S. (1996). Compressibility and flow parameters form PVD improved soft Bangkok clay. Geotech. Eng’g. Journal, 27(1), 1-20.
Blauw, A. N., Los, F.J., Huisman, J., Peperzak, L. (2010). Nuisance foam events and Phaeocystis globosa blooms in Dutch coastal waters analyzed with fuzzy logic. Journal of Marine Systems, 83 (2010) 115–126.
Chou, C.W., Seagren, E.A., Aydilek, A.H., and Lai, M. (2011). Biocalcification of Sand through Urelysis. Journal of Geotechnical and Geoenvironmental Engineering (ASCE),127(12), 1179-1189.
DeJong, J. T., Fritzges, M. B., and Nüsslein, K. (2006). Microbially induced cementation to control sand response to undrained shear. Journal of Geotechnical and Geoenvironmental Engineering, 132(11), 1381-1392.
DeJong, J.T., Mortensen, B.M., Martinez, B.C., and Nelson, D.C. (2008). Bio-mediated soil improvement. Journal of Ecological Engineering, 197-210.
DeJong, J.T. (2010). Bio-mediate soil improvement. Journal of Ecological Engineering, (36), 197-210.
Grini, A. G., Myhre Zender, C. S., Sundet, J. K., and Isaksen, I. S. A. (2003). Model simulations of dust source and transport in the global troposphere: Effects of soil erodibility and wind speed variability. Institute Report. No. 124, Norway, University of Oslo, Department of Geosciences.
Henry, D.F. (2006) Fundamentals of Soil Science. (8th ed). New York: Wiley.
Ingaki, Y., Tsukamoto, M., Mori, H., Nakajiman, S., Sasaki, T., and Kawasaki, S. (2011). A Centrifugal Model Test of Microbial Carbonate Precipitation as Liquefaction Countermeasure. Jiban Kogaku Janaru (Journal of Japanese Geotechnical), 6(2), 157-167.
Ivanov, V., Chu, J. (2008). Applications of microorganisms to geotechnical engineering for bioclogging and biocementation of soil in situ. Rev. Environ. Sci. Biotechnol, 7, 139–153.
Jalalian, A., Ghahsareh, A.M., and Karimzadeh, H.R. (1995). Soil erosion estimates for some watersheds in Iran. land degradation and desertification newsletter of the International Task Force on Land Degradation, NRCS.
Karol, R.H. (2003) Chemical grouting and soil stabilization. 3rd edn. M. Dekker, New York.
Kazemi, M.M. (2013). Investigating the parameters affecting the biological fixation using microalgae Microalgae chlorella vulgaris in Fine-grained soil. MS Thesis, Shiraz University, Shiraz, Iran. (In Farsi)
Laity, J. (2008) Deserts and desert environments. (1th ed). Willey-Blackwell.
Martinez, B.C., Barkouki, T.H., DeJung, J.T., and Ginn, T.R. (2011). Upscaling of Microbial Induced Calcite Precipitation in 0.5m Columns: Experimental and Modeling Results. ASCE Geo-Institute Annual Conference: Geo-Frontiers 2011. Dallas, Texas, 2011, pp. 4049-4059.
Mitchell, J.K., and Santamarina, J.C. (2005). Biological considerations in geotechnical engineering. Journal of Geotechnical and Geoenvironmental Engineering, 131(10), 1222–1233.
Montoya, B. M., DeJong, J. T., and Boulanger, R. W. (2013). Dynamic response of liquefiable sand improved by microbial-induced calcite precipitation. Journal of Geotechnique, 63(4), 302-312.
Moravej, S., Habib Agahi, Gh., Niazi, A. (2013). Stabilization of Divergent soil using Bacillus Asfarykvs. The first national conference of Iranian Geotechnical Engineering, School of Engineering, University of Ardabil, Iran. (In Farsi)
Pimental, D., Allen, J., Beers, A., Guinand, L., Linder, R., Mc- Laughlin, P., Meer, B., Musonda, D., Perdue, D., Poisson, S., Siebert, S., Stoner, K., Salazar, R., and Hawkins, A. (1987). World agriculture and soil erosion. School of Biological Sciences, 37, 277-282.
Ramachandran, S.K., Ramakrishnan, V., and Bang, S.S. (2001). Remediation of concrete using micro-organisms. Journal of ACI Materials, 98(1), 3-9.
Rebata-Landa, V. (2007). Microbial Activity in Sediments: Effects on Soil Behavior. Ph .D. dissertation, Georgia Institute of Technology, Atlanta, GA, USA.
Rusu, C., Cheng, X., and Li, M. (2011). Biological Clogging in Tangshan Sand Columns under Salt Water Intrusion by Sporosarcina Pasteurii. Journal of Advanced Material Research,250-253, 2040-2046.
Saffari, R., Habib Agahi, Gh., Nekoei, E., Niazi, A. (2015).  Effect of biological stabilized soil in soil - water retention curve.10th International Congress of Civil Engineering, Tabriz, Tabriz University, Department of Civil Engineering, Iran. (In Farsi)
Sajjadi, M., Habib Agahi, Gh., Niazi, A., Nekoei, E. (2013). Sowelling controll of expansive soil through the microbial calcite deposition. National Conference of applied Civil Engineering with Modern achievements, Kavir Sazeh Company, Karaj, Iran. (In Farsi)
Shahrokhi-Shahraki, R., Zomorodian, S.M.A., Niazi, A., and O'Kelly, B.C. (2014). Improving sand with microbial-induced carbonate precipitation. Journal of Proceedings of the Institution of Civil Engineers, Ground Improvement, 168(3), 217- 230. 
Stocks-Fischer, S., Galinat, J.K., and Bang, S.S. (1999). Microbiological precipitation of CaCO3. Journal of Soil Biology and Biochemistry, 31(11), 1563-1571.
Troeh, F.R., Hobbs, J.A., and Donahue, R.L. (1980). Soil and Water Conservation for Productivity and Environmental protection. Prentice - Hall, Englewood Cliffs, NJ.
United Nations Environment Program (UNEP) and International Soil Research Information Center (ISRIC). (1990). World map of the status of human induced soil degradation.
Van Paassen, L. A. (2011). Bio-mediated ground improvement: from laboratory experiment to pilot applications. Proc.
GeoFrontiers: Advances in Geotechnical Engineering, Dallas, TX, ASCE Geotechnical Special Publication, 211, 4099– 4108.
Whiffin, V.S. (2004). Microbial CaCO3 precipitation for the production of biocement. School of biological sciences and biotechnology, Murdoch University, Western Australia.
Whiffin, V.S., van Paassen, L.A., and Harkes, M.P. (2007). Microbial carbonate precipitation as a soil improvement technique. Journal of Geomicrobiology, 24(5), 417-423.
Xuan, J., Sokolik, I., Hao, J., and Guo, F. (2004). Identification and characterization of source of atmospheric mineral dust in East Asia. Journal of Atmospheric Enviromental, 38(36), 6239-6252.