Use of Arbuscular Mycorrhizal fungi for more effective bioremediation of contaminated soil using both corn and sunflower

Document Type : Research Paper

Authors

Scientific Staff of Soil Biology Department

Abstract

One of the method to clean up contaminated soil is growing a hyper accumulator plant which is named phytoremediation. Some of  hyper accumulator plants are proper host for mycorrhizal fungi.In this relationship, fungus, consume a part of organic carbon from the plant and by developing its mycelium network help plant to uptake more mineral nutrients from the soil. By doing a greenhouse research tried to determine the mycorrhizal efficiency to increase plant ability in mineral nutrient and heavy metal uptake from a contaminated soil with a high level of Cr, Ni  and Cd .In this exam two hyper accumulator plants , sunflower and corn and also 4 species of mycorrhizal fungi, including, Glomus mosseae , Glomus intraradices ,Glomus etunicatum and Glomus sp and one treatment without fungi as blank, with 5 replications per treatment were used. Results showed that mycorrhizal inoculation in two plants , sunflower and corn by increasing mycorrhizal colonization enhanced aerial part growth of plant and also macro and micro nutrient uptake. T4 treatment, including G.mosseae and G.intraradices in corn growth had the highest capacity for Cd, Ni and Cr uptake from the soil, so that it increased the uptake of these three elements in corn stalks react, 1.57, 3.79 and 4.18 micrograms per pot. In sunflower this treatment was able to increase Cd and Ni uptake, react 0.21 and 0.23 micrograms per pot. Finally if we want to use these two plants for phytoremediation, the use of these two fungi will be proposed.

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Ali –Ehyaiee, M. (1997). Methods of chemical analysis of soil. Technical publication, No. 1024, (Vol.2), Soil and Water Research Institute, Tehran. (In Farsi).
Al-Karaki, G. N. and Al-Raddad, A. (1997). Effects of arbuscular mycorrhizal fungi and drought stress on growth and nutrient uptake of two wheat genotypes differing in drought resistance, Mycorrhiza, 7, 83-88.
Al-Karaki, G. N., Al-Raddad, A. and Clark, R. B. (1998). Water stress and mycorrhizal isolates effects on growth and nutrient acquisition of wheat, Journal of Plant Nutrition, 21, 891-902.
Baker, A. J. M. Reeves, R. D. Hajar, A. S. M. (1994). Heavy metal accumulation and tolerance in British populations of the metallophyte Thlaspi caerulescens J.&C. Presl (Brassicaceae). New Phytol, 127, 61-68.
Baker, D. E., Amachar, M. C. (1982). Nickel, copper, zinc and cadmium. In: Page A. L., Miller, R. H., Keeney, D. R., editors. Methods of soil analysis, part 2.  American Society of Agronomy, Madison, 323–338.
Blaylock, M. Huang, J. (2000). Phytoextraction of Metals. In: Raskin I, Ensley B (eds) Phytoremediation of toxic metals: Using plants to clean up the environment. Wiley Interscience, New York, pp 53-70.
Cassel, D. K., and Nielsen, D. R. (1986). Field capacity and available water capacity, Pp. 901-926. In: Klute A (ed). Methods of Soil Analysis. Part 1: Physical and Mineralogical Methods, 2nd. American Society of Agronomy and Soil Science Society of America, Madison, WI.
Chapman, H. D., and Pratt, P. F. (1961). Methods of analysis for soils, plants and waters. Division of Agricultural Sciences, University of California, Riverside.
Chaudhry, T. M. Hill, L. Khan, A. G. Kuek, C. (1999). Colonization of iron and zinc-contaminated dumped ®ltercake waste by microbes, plants and associated mycorrhizae. In: Wong, M.H. Wong, J.W.C. Baker, A.J.M. (Eds.), Remediation and Management of  Degraded Land. CRC Press LLC, Boca Raton. pp. 275-283.
Clark, R. B. (1997). Arbuscular mycorrhizal adaptation, spore germination, root colonization, and host plant growth and mineral acquisition at low pH. Plant and Soil, 192, 15-22.
Clark, R. B., and Zeto, S. K. (1996). Mineral acquisition by mycorrhizal maize grown on acid and alkaline soil. Soil Biology and Biochemistry, 28, 1405-1503.
Cottenie, A. (1998). Soil and plant testing as a basis of fertilizer recommendations F.A.O soil Bulletin, 38/2.
Cunningham, S. D. Berti, W. R., Huang, J. W. (1995). Phytoremediation of contaminated soils. Tibitech. 13, 393–397.
Emami, A. (1996). Methods of chemical analysis of plant. Technical publication, No. 982, (Vol.1), Soil and Water Research Institute, Tehran. (In Farsi). 
Forstner, U. (1995). Land contamination by metals: global scope and magnitude of problem. In: Allen, H.E. Huang, C.P. Bailey, G.W. Bowers, A.R. editors. Metal speciation and contamination of soil. Boca Raton, FL: CRC Press, p. 1–33.
Garbisu, C. Alkorta, I. (2003). Basic concepts on heavy metal soil bioremediation. Eur J. Min Proc. Environ. Protect. 13, 58–66.
Gee, G. W., and Bauder, J. W. (1986). Particle-size analysis. In: Klute A(ed) Methods of soil Analysis, Part 1. 2 ed., Agronomy Monograps. American Society of Agronomy and Soil science Society, Madison, 9: 383-411
Giovannetti, M., Mosse, B. (1980). An evaluation of techniques for measuring vesicular-arbuscular infection in roots. New Phytologis,t 84, 489-500.
Glass, D. J. (1999). U.S. and International Markets for Phytoremediation, 1999–2000. Needham, MA: D. Glass A.
Halim, M. Conte, P. Piccolo, A. (2003). Potential availability of heavy metals to phytoextraction from contaminated soils induced by exogenous humic substance, Chemosphere, 52(1), 265–75.
Heggo, A., Angle, J. S., Chaney, R. L. (1990). Effects of vesicular-arbuscular mycorrhizal fungi on heavy metal uptake by soybeans. Soil Biology & Biochemistry , 22, 865–869.
Isac , A. (1990). Associate chapter editor, Methods of plant analysis, official Methods of Analysis of the A.O.A.C.
Jackson, M. L. (1967). Soil Chemical analysis. Prentice Hall of India, Private limited, New Delhi, p. 498.
Khan, A. G. (2003). Vetiver grass as an ideal phytosymbiont for Glomalian fungi for ecological restoration of derelict land. In: Truong, P. Hanping, X. editors. Proceedings of the third international conference on vetiver and exhibition: vetiver and water, Guangzou, China, p.466–74.
Khan, A. G. Kuek, C. Chaudhry, T. M. Khoo, C. S. Hayes, W. J. (2000). Role of plants, mycorrhizae and phytochelators in heavy metal contaminated land remediation. Chemosphere, 41, 197– 207.
Knasmuller, S., Gottmann, E., Steinkellner, H., Fomin, A., Pickl, C., God, R. and Kundi, M. (1998). Detection of genotoxic effects of heavy metal contaminated soils with plant bioassays. Mutation Research. 420, 37-48.
Knudsen, D., Peterson, G. A., and Pratt, P. F. (1982). Lithium, sodium, and potassium. In A. L. Page, R. H. Miller, and D. R. Keeney, editors. Methods of soil analysis, part 2. Chemical and microbiological properties. American Society of Agronomy, Madison, Wisconsin, USA. 225–246
Kumar, P. B., Nanda, A., Dushenkov, V., Motto, H.,  Raskin, I. (1995). Phytoextraction: The use of plants to remove heavy metals from soils. Environ. Sci. Technol, 29(5), 1232-1238.
Leyval, C., Turnau, L. C.,   Haselwandter, K. (1997). Effect of heavy metal pollution on mycorrhizal colonization and function: physiological, ecological and applied aspects, Mycorrhiza, 7, 139–53.
Liao, J. P., Lin, X. G., Cao, Z. H., Shi, Y. Q. and Wong, M. H. (2003). Intraction between arbuscular mycorrhizae and heavy metals under sand culture experiment. Chemosphere, 50, 847-853.
Liao, J. P., Lin, X. G., Cao, Z. H., Shi, Y. Q., Wong, M. H. (2003). Intraction between arbuscular mycorrhizae and heavy metals under sand culture experiment, Chemosphere, 50, 847-853.
Linger, P., Mussing, J., Fischer, H., Kobert, J. (2002). Industrial hemp (Cannabis sativa L.) growing on heavy metal contaminated soil: fibre quality and phytoremediation potential, Ind Crops Prod, 16, 33–42.
Long, X. X., Yang, X. E., Ni, W. Z. (2002). current status and perspective on phytoremediation of heavy metal polluted soils. Journal of Applied Ecology, 13, 757–62.
Manteghi,  N. (1365). Describe methods and  laboratory studies on soil and water samples, No. 168, Soil and Water Research Institute, Tehran. (In Farsi). 
Marschner, H. (1995). Mineral nutrition of higher plants. San Diego, CA: Academic Press.
McIntyre, T. (2003). Phytoremediation of heavy metals from soils. Adv Biochem Eng Biotechnol, 78, 97–123.
Nelson, D. W., and Sommers. L. E. (1982). Total carbon, organic carbon and organic matter. In: page AL, Miller RH, Keenney DR (eds) Methods of soil analysis, part 2, 2nd edn. American Society of Agronomy, Madison, Wisconsin, 539-573.
Nelson, D. W., Sommers, L. E. (1973). Determination of total nitrogen in plant material. Agron. J., 65: 109- 112.
Newman E. I. (1966). A method of estimating the total length of root in a sample. Journal of Applied Ecology, 3, 139-145.
Olsen, R. S. (1954). Estimation of available phosphorus in soils by extraction with sodium bicarbonate. US Department of Agriculture, p. 939.
Perkin, E. (1982). Analytical Methods for Atomic Absorption Spectrophotometry, Norwalk, CT, January.
Pilon-Smits, E., Pilon, M. (2000). Breeding mercury-breathing plants for environmental clean-up, Trends Plant Sci., 5, 235-236.
Pivetz, B. (2001). Phytoremediation of Contaminated Soil and Ground Water at Hazardous Waste Sites. Ground Water Issue. United States Environmental Protection Agency.
Raskin, I., Kumar, P. B. A. N., Dushenkov, V., Salt, D. E. (1994). Bioconcentration of heavy metals by plants. Current Opin. Biotech, 5, 285-290.
Rejali, F. (2004). Identification of indigenous arbuscular mycorrhizal fungi and determination of their ability to colonize dry land wheat. Final report, no. 1515. Soil and Water Research Institute, Karaj. (In Farsi).
Rhoades, J. D. (1982). Cation exchange capacity.  in A. L. Page, R. H. Miller, and D. R. Keeney(ed), Methods of soil analysis, part 2. Chemical and microbiological properties. American Society of Agronomy, Madison, Wisconsin, USA. 149–158.
Saggin, O. J., and Siqueira, J. O. (1995). Evaluation of the symbiotic effectiveness of endomycorrhizal fungi for coffee tree. Brazil Journal of Soil Science, 19, 221-228.
Salt, D. E., Blaylock, M., KumarNanda, P. B. A., Dushenkov, V., Ensley, B. D., Chet, I., Raskin, I. (1995). Phytoremediation: A novel strategy for the removal of toxic metals from the environment using plants. Biotechnol, 13, 468-474.
Sharma, A. K. and Johri, B. N. (eds.). (2002). Arbuscular Mycorrhizae, Interaction in Plants, Rhizosphere and Soils. Oxford and IBH Publishing, New Delhi, p. 308.
Shetty, K. G., Bank, M. K., Hertrik, B. A., Schwab, A. P. (1995). Effects of mycorrhizae and fertilizer amendments on Zinc tolerance of plants. Environmental Pollution, 88, 307-314.
Sieverding, E., and Toro, T. S. (1988). Influence of soil water regimes on VA mycorrhiza. V. Performance of different VAM fungal species with cassava. Journal of Agronomy and Crop Science, 161, 322-332.
Tennant, D. (1975). A test of a modified line intersection method of measuring root length. Journal of Ecology, 63, 995-1001.
Tessier, A. P. G., Campbell, C. and Bisson, M. (1979). Sequential extraction  procedure for the speciation of particulate trace metals. Anal. Chemosphere, 51, 844-850.
Waling, I., Van Vark, W., Houba, V. J. G., and Van der lee, J. J. (1989). Soil and plant analysis, a series of syllabi part7. Plant Analysis procedures. Wageningen Agriculture University.
Weissenhorn, I., Leyval, C. (1995). Root colonization of maize by a Cd-sensitive and a Cd-tolerant Glomus mosseae and cadmium uptake in sand culture. Plant Soil, 175, 233-238.
Xiong, L. M. (1993). Vesicular- arbuscular mycorrhizae decrease cadmium uptake by plant. Journal of plant Resources and Environment, 2 (3), 58-60.