To study the effects of biofertilizers in their different levels of nitrogen (N) and phosphorus (P) on yield related parameters of barley (Sahra cultivar), a split plot experiment based upon a randomized complete block design of three replications was conducted. Main plots consisted of: nitrogen at three levels (0, 50 and 100 kg N ha-1 as urea), while sub plots were: phosphorus at two levels (0 and 50 kg P2O5 ha-1 as triple super phosphate). Sub sub plots consisted of: biofertilizers at four levels (control, inoculation of phosphate solubilizing bacteria or PSM, inoculation of Nitrogen Fixation Rhizobacteria or NFB along with co-inoculation of PSM and NFB). Results revealed that N, P biofertilizer and interaction of N as well as P were highly significant in terms of all the yield related parameters. Mean comparison indicated that increasing of N with inoculation of biofertilizers coud markedly improve grain yield in which maximum grain yield (364.5 gm2) was recorded when the application of 100 kg of Nha-1 plus co-inoculation of PSM and NFB biofertilizers were involved.