Effect of Selenium-Enriched Biochar on Some Chemical Properties of Calcareous Soil Under Salinity Stress

Document Type : Research Paper

Authors

1 Department of Soil Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Khuzestan

2 Department of Soil Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Khuzestan, Iran

10.22059/ijswr.2024.380534.669776

Abstract

A basic solution to reduce the harmful effects of salinity stress is to apply biochar, which can be improved by modifying and enriching it. For this purpose, a completely randomized design experiment with three replications was conducted with treatments including salinity (2 and 6 dS/m) and biochar prepared from landscape waste at four levels (zero and 5% without enrichment, 5% enrichment with selenium at the rate of 20 and 60 mg/kg). Biochar enrichment decreased soil pH. Enriched biochar at the level of 60 mg/kg increased soil salinity. The amount of soil organic carbon increased proportionally by increasing biochar. The lowest amount of phosphorus was observed in the treatment of saline soil without biochar at the rate of 2.47 mg/kg, and the highest amount was observed in the treatment of non-saline soil containing 5% enriched biochar at the level of 60 mg/kg at the rate of 3.55 mg/kg. The amount of available potassium showed an increasing trend with increasing salinity levels. Enriching biochar with selenium reduced the positive effects of biochar on increasing the amount of potassium, especially in saline soil. In non-saline soil, biochar enrichment increased soluble sodium by 16.6% compared to non enrichment biochar, and increasing the level of enrichment did not show a significant effect. The change in the amount of solutes in the soil changed its chemical characteristics, and these changes were manifested in different ways under the influence of biochar and the level of enrichment.

Keywords

Main Subjects


EXTENDED ABSTRACT

 

Introduction

Soil salinity is one of the main stresses, especially in arid and semi-arid regions. It significantly reduces available land, production, and quality of agricultural products. Khuzestan province, with five percent of the country's soil resources, is one of the most suitable regions of Iran for agricultural development. The presence of salts in the parent materials, the high level of underground water, high evaporation, and low rainfall have caused the lands of Khuzestan to need intensive management to deal with salinity problems. One basic strategy for reducing the negative effects of salinity stress on plants is to use biochar. selenium has antioxidant properties and neutralizes oxygen free radicals in stress conditions, including salt stress. Considering that most past research has investigated the effects of biochar and enriched biochar on growth and plant production under different stresses, this study was considered to investigate the effect of selenium-enriched biochar on some chemical properties of soil under salinity stress.

Materials and Methods

The experiment was conducted in a completely randomized design with three replications. For this purpose, a completely randomized design experiment with three replications was conducted with treatments including salinity (2 and 6 dS/m) and biochar prepared from landscape waste at four levels (zero (B0), 5% without enrichment (B5), 5% enrichment with selenium at the rate of 20 (B5Se20) and 60 mg/kg (B5Se60)). The required agricultural soil was collected from 0-30 cm depth. Basic physical and chemical properties were measured after passing the soil through a 2 mm sieve. At the end of the 120-day incubation period at 25°C, the moisture of field capacity, soil reaction in saturated paste, electrical conductivity in saturated extract, organic carbon, and concentration of phosphorus, potassium, and sodium were measured again. This research data analysis was done using SPSS software. Means were compared using Duncan's method at the 5% probability level. Excel software was used to draw graphs.

Results and Discussion

Enriching biochar with selenium reduced soil pH. Applying biochar enriched with selenium at 60 mg/kg increased soil salinity. The amount of organic carbon in the soil increased in proportion to the addition of biochar, which, according to the available reports, more consumption amounts cause a more significant increase in the content of organic carbon in the soil. Enrichment of biochar with selenium increased the effect of biochar on increasing the amount of phosphorus in the soil. With the increase in enrichment, a decreasing trend was observed in saline soil. The amount of available potassium showed an increasing trend with the increase in salinity level The enrichment of biochar with selenium reduced the positive effects of biochar on increasing the amount of potassium, especially in saline soil. In saline soil, adding biochar decreased the amount of soluble sodium, and the amount of enrichment changed the effectiveness of biochar.

Conclusion

Changing the amount of solutes in the soil can change its chemical properties, and these changes manifest in different ways under the influence of biochar and the level of enrichment of biochar with selenium. According to this research, biochar enrichment at 20 mg/kg is suggested to increase biochar's efficiency in reducing salinity's adverse effects on the considered chemical properties.

Author Contributions

All authors contributed equally to the conceptualization of the article and writing of the original and subsequent drafts.

Data Availability Statement

Data available on request from the authors.

Acknowledgements

The authors would like to thank all participants of the present study.

Ethical considerations

The authors avoided data fabrication, falsification, plagiarism, and misconduct.

Conflict of interest

The author declares no conflict of interest.

Al-Wabel, M.I., Al-Omran, A., El-Naggar, A.H., Nadeem, M. & Usman, A.R. (2013). Pyrolysis temperature induced changes in characteristics and chemical composition of biochar produced from conocarpus wastes. Bioresource Technology, 131, 374-379.‏ https://doi.org/10.1016/j.biortech.2012.12.165.
Anegbe, B., Okuo, J.M., Ewekay, E.O., & Ogbeifun, D.E. (2015). Fractionation of lead-acid battery soil amended with Biochar. Bayero Journal of Pure and Applied Sciences, 7(2), 36. https://doi.org/7. 36. 10.4314/bajopas.v7i2.8.
Chapman, H.D. (1965). Cation Exchange Capacity. In: Black, C.A., Ed., Methods of Soil Analysis. American Society of Agronomy, Madison, 891-901.
Chhabra, R. (2004). Classification of salt-affected soils. Arid Land Research and Management, 19, 61-79. https://doi.org/10.1080/15324980590887344.
Chia, CH., Singh, BP., Joseph, S., Graber, ER., & Munroe, P. (2014). Characterization of an enriched biochar. Journal of Analytical and Applied Pyrolysis, 108, 26-34. https://doi.org/10.1016/j.jaap.2014.05.021.
Chintala, R., Mollinedo, J., Schumacher, T.E., Papiernik, S.K., Malo, D.D., Clay, D.E., Kumar, S., & Gulbrandson, D.W. (2013). Nitrate sorption and desorption in biochars from fast pyrolysis. Microporous and Mesoporous Materials, 179, 250-257. https://doi.org/10.1016/j.micromeso.2013.05.023
Clark, J.S. (1964). An examination of the pH of calcareous soils. Soil Science, 98(3), 145-151. https://doi.org/10.1097/00010694-196409000-00001.
Cresser, M., Killham, K., & Edwards, Tony. (1993). Soil Chemistry and Its Applications. Cambridge University Press. 10.1017/CBO9780511622939.
Demir Kaya, M., Okçu, G., Atak, M., Yakup Çıkılı, Y., & Özer Kolsarıcı, O. (2006). Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24 (4), 291-295. https://doi.org/10.1016/j.eja.2005.08.001.
Duan, S., & Kaushal, S.S. (2015). Salinization alters fluxes of bioreactive elements from stream ecosystems across land use. Biogeosciences, 12, 7331-7347. https://doi.org/10.5194/bg-12-7331-2015.
Elzobair, KA., Stromberger, ME., Ippolito, JA., & Lentz, RD. (2016) Contrasting effects of biochar versus manure on soil microbial communities and enzyme activities in an Aridisol. Chemosphere, 142, 145-52. https://doi.org/10.1016/j.chemosphere.
Gee, G.W., & Bauder, J.W. (1986). Particle-Size Analysis. In: Klute, A., Ed., Methods of Soil Analysis, Part 1. Physical and Mineralogical Methods, Agronomy Monograph. No. 9, 2nd Edition, American Society of Agronomy/Soil Science Society of America, Madison, WI, 383-411.
Haefele, S. M., Konboon, Y., Wongboon, W., Amarante, S., Maarifat, A. A., Pfeiffer, E. M., & Knoblauch, C.J.F.C.R. (2011). Effects and fate of biochar from rice residues in rice-based systems. Field Crops Research, 121(3), 430-440. https://doi.org/10.1016/j.fcr.2011.01.014.
Hamam, Kh., & Negim, O. (2014). Evaluation of wheat genotypes and some soil properties under saline water irrigation. Annals of Agricultural Sciences. https://doi.org/59.10.1016/j.aoas.2014.11.002.
Hammer, EC., Forstreuter, M., Rillig, MC., & Kohler, J. (2015). Biochar increases arbuscular mycorrhizal plant growth enhancement and ameliorates salinity stress. Applied Soil Ecology, 96, 114-121. https://doi.org/10.1016/j.apsoil.2015.07.014.
Hashim, B.A., & Mohammed, H.A. (2023). Effect of adding potassium and selenium on the concentration of nutrients in climbing bean plant (Phaseolus vulgaris) affected by humidity stress. In IOP Conference Series: Earth and Environmental Science, 1262 (8), 082027. IOP Publishing. https://doi.org/10.1088/1755-1315/1262/8/082027.
Hawrylak-Nowak, B. (2008). Effect of Selenium on selected macronutrients in maize plants. Journal of Elementology, 13, 513-519.
Hejazizadeh, A., Gholamalizadeh Ahangar, A., & Ghorbani, M. (2016). Effect of Biochar on Lead and Cadmium Uptake from Applied Paper Factory Sewage Sludge by Sunflower (Heliantus annus L.). Water and Soil Science, 26 (1-2), 259-271. (In Persian).
Hinsinger, P., Plassard, C., Tang, C., & Jaillard, B. (2003). Origins of root-mediated pH changes in the rhizosphere and their responses to environmental constraints: A review. Plant and Soil, 248, 43-59. https://doi.org/10.1007/978-94-010-0243-1_4.
International Biochar Initiative. (2015). Standardized Product Definition and Product Testing Guidelines for Biochar That Is Used in Soil. IBI-STD-2.1. https://biochar-international.org/wp-content/uploads/2020/06/IBI_Biochar_Standards_V2.1_Final2.pdf.
Ippolito, J., Laird, D., & Busscher, W. (2012). Environmental benefits of biochar. Journal of Environmental Quality, 41, 967-72. https://doi.org/10.2134/jeq2012.0151.
Jackson, M.L. (1973). Soil Chemical Analysis. Prentice Hall of India Pvt. Ltd., New Delhi, 498.
Jalali, M. (2008). Effect of sodium and magnesium on kinetics of potassium release in some calcareous soils of western Iran. Geoderma, 145(3-4), 207-215. https://doi.org/10.1016/j.geoderma.2008.03.005.
Jalali, M., & Ranjbar, F. (2009). Effects of sodic water on soil sodicity and nutrient leaching in poultry and sheep manure amended soils, Geoderma, 153 (1-2), 194-204. https://doi.org/10.1016/j.geoderma.2009.08.004.
Kaur, S., & Nayyar, H. (2015).Selenium fertilization to salt-stressed mungbean (Vigna radiata L. Wilczek) plants reduces sodium uptake, improves reproductive function, pod set and seed yield. Scientia Horticulturae, 197, 304-317. https://doi.org/10.1016/j.scienta.2015.09.048.
Keling, H., Ling, Z., JiTao, W., & Yang, Y. (2013). Influence of selenium on growth, lipid peroxidation and antioxidative enzyme activity in melon (Cucumis melo L.) seedlings under salt stress. Acta Societatis Botanicorum Poloniae, 82(3), 193-197. https://doi.org/10.5586/asbp.2013.023.
Khan, K.T., Chowdhury, M.T.A., & Huq, S.I. (2014). Application of biochar and fate of soil nutrients. Bangladesh Journal of Scientific Research, 27(1), 11-25. https://doi.org/10.3329/bjsr.v27i1.26221.
Kim, H.S., Kim, K.R., Yang, J.E., Ok, Y.S., Owens, G., Nehls, T., Wessolek, G., & Kim, K.H. (2016). Effect of biochar on reclaimed tidal land soil properties and maize (Zea mays L.) response. Chemosphere, 142, 153-159. https://doi.org/10.1016/j.chemosphere.2015.06.041.
Lai R., & Stewart BA. (1990). Salt-affected soils. In Soil Degradation. Springer-Verlag: New York; 224-247.
Lashari, M. S., Liu, Y., Li, L., Pan, W., Fu, J., Pan, G., & Yu, X. (2013). Effects of amendment of biochar-manure compost in conjunction with pyroligneous solution on soil quality and wheat yield of a salt-stressed cropland from Central China Great Plain. Field Crops Research, 144, 113-118. https://doi.org/10.1016/j.fcr.2012.11.015.
Lashari, M.S., Bakht-un-Nisa Mangan, I.R., Ji, H., Pan, G., Lashari, A.A., & Nan, J. (2018). Improvement of soil fertility and crop yield through biochar amendment from salt affected soil of central china. Journal of Agricultural Science and Technology, 8, 209. https://doi.org/10.17265/2161-6264/2018.04.002.
Lehmann, L., Matthias, C., Rillig, JT., Caroline, A., Masiello, W.C., & Hockaday, D.C. (2011). Biochar effects on soil biota - A review. Soil Biology and Biochemistry, 43(9), 1812-1836. https://doi.org/10.1016/j.soilbio.2011.04.022.
Lentz, R.D., & Ippolito, J.A. (2012). Biochar and manure affect calcareous soil and corn silage nutrient concentrations and uptake. Journal of Environmental Quality, 41(4), 1033-1043. https://doi.org/10.2134/jeq2011.0126.
Li, Y., Xing, B., Ding, Y., Han, X., & Wang, Sh. (2020). A critical review of the production and advanced utilization of biochar via selective pyrolysis of lignocellulosic biomass. Bioresource Technology, 312, 123614. https://doi.org/10.1016/j.biortech.2020.123614.
Lin, X.W., Xie, Z.B., Zheng, J.Y., Liu, Q., Bei, Q.C., & Zhu, J.G. (2015). Effects of biochar application on greenhouse gas emissions, carbon sequestration and crop growth in coastal saline soil. European Journal of Soil Science, 66(2), 329-338. https://doi.org/10.1111/ejss.12225.
Lindsay, W.L., & Norvell, W.A. (1978). Development of a DTPA Soil Test for Zinc, Iron, Manganese, and Copper. Soil Science Society of America Journal, 42, 421-428. https://doi.org/10.2136/sssaj1978.03615995004200030009x.
Liu, W., Huo, R., Xu, J., Liang, S., Li, J., Zhao, T., & Wang, S. (2017). Effects of biochar on nitrogen transformation and heavy metals in sludge composting. Bioresource Technology, 235, 43-49. https://doi.org/10.1016/j.biortech.2017.03.052.
Liu, X.H., & Zhang, X.C. (2012). Effect of biochar on pH of alkaline soils in the loess plateau: results from incubation experiments. International Journal of Agriculture & Biology, 14, 745-750.
Luo, X., Liu, G., Xia, Y., Chen, L., Jiang, Z., Zheng, H., & Wang, Z. (2017). Use of biochar-compost to improve properties and productivity of the degraded coastal soil in the Yellow River Delta, China. Journal of Soils and Sediments, 17, 780-789. https://doi.org/10.1007/s11368-016-1361-1.
Ma, N., Zhang, L., Zhang, Y., Yang, L., Yu, C., Yin, G., Doane, T.A., Wu, Z., Zhu, P., & Ma, X. (2016). Biochar improves soil aggregate stability and water availability in a mollisol after three years of field application. PLOS ONE, 11(5), p.e0154091. https://doi.org/10.1371/journal.pone.0154091.
Mahrous, F.N., Mikkelsen, D.S., & Hafez, A.A. (1983). Effect of soil salinity on the electro-chemical and chemical kinetics of some plant nutrients in submerged soils. Plant and Soil, 75, 455-472. https://doi.org/10.1007/BF02369980.
Masto, R.E., Ansari, M.A., George, J., Selvi, V.A., & Ram, L.C. (2013). Co-application of biochar and lignite fly ash on soil nutrients and biological parameters at different crop growth stages of Zea mays. Ecological Engineering, 58, 314-322. https://doi.org/10.1016/j.ecoleng.2013.07.011.
McGeorge,W.T. (1938). Factor contributing to the reaction of soil sand their pH measurement. Arizona Agricultural Experimental Station Technical Bulletin, 78, 95-126.
Mia, S., Dijkstra, F.A., & Singh, B. (2017). Long-term aging of biochar: a molecular understanding with agricultural and environmental implications. Advances in Agronomy, 141, 1-51. https://doi.org/10.1016/bs.agron.2016.10.001.
Mukherjee, A., & Zimmerman, A.R. (2013). Organic carbon and nutrient release from a range of laboratory-produced biochars and biochar-soil mixtures. Geoderma, 193-194, 122-130. https://doi.org/10.1016/j.geoderma.2012.10.002.
Naeem, M.A., Khalid, M., Aon, M., Abbas, G., Amjad, M., Murtaza, B., Khan, W.U.D., & Ahmad, N. (2018). Combined application of biochar with compost and fertilizer improves soil properties and grain yield of maize. Journal of Plant Nutrition, 41(1), 112-122. https://doi.org/10.1080/01904167.2017.1381734.
Naidu, R., Syers, J.K., Tillman, R.W., & Kirkman, J.H. (1991). Assessment of plant-available phosphate in limed, acid soils using several soil-testing procedures. Fertilizer Research, 30, 47-53. https://doi.org/10.1007/BF01048826.
Nawaz, F., Ahmad, R., Ashraf, MY., Waraich, EA., & Khan SZ. (2015). Effect of selenium foliar spray on physiological and biochemical processes and chemical constituents of wheat under drought stress. Ecotoxicology Environmental Safety, 113, 191-200. doi: 10.1016/j.ecoenv.2014.12.003.
Nelson, R.E., (1982). Carbonate and Gypsum. Agronomy Journal, 9, 181-197.
O’toole, A., Moni, C., Weldon, S., Schols, A., Carnol, M., Bosman, B., & Rasse, D.P. (2018). Miscanthus biochar had limited effects on soil physical properties, microbial biomass, and grain yield in a four-year field experiment in Norway. Agriculture, 8(11), 171. https://doi.org/10.3390/agriculture8110171.
Obia, A., Mulder, J., Martinsen, V., Cornelissen, G., & Børresen, T. (2016). In situ effects of biochar on aggregation, water retention and porosity in light-textured tropical soils. Soil and Tillage Research, 155, 35-44. https://doi.org/10.1016/j.still.2015.08.002.
Olsen, S.R., Cole, C.V., & Watanabe, F.S. (1954). Estimation of Available Phosphorus in Soils by Extraction with Sodium Bicarbonate. USDA Circular No. 939, US Government Printing Office, Washington DC.
Pietikäinen, J., Kiikkilä, O., & Fritze, H. (2000). Charcoal as a habitat for microbes and its effect on the microbial community of the underlying humus. Oikos, 89. 231-242. https://doi.org/10.1034/j.1600-0706.2000.890203.x.
Quevauviller, PH., (1998). Operationally defined extraction procedures for soil and sediment analysis I. Standardization. Trends in Analytical Chemistry, 17(5), 289-298. https://doi.org/10.1016/S0165-9936(97)00119-2.
Rafique, M., Ortas, I., Rizwan, M., Chaudhary, H.J., Gurmani, A.R., & Munis, M.F.H. (2020). Residual effects of biochar and phosphorus on growth and nutrient accumulation by maize (Zea mays L.) amended with microbes in texturally different soils. Chemosphere, 238, 124710. https://doi.org/10.1016/j.chemosphere.2019.124710.
Rajkovich, S., Enders, A., Hanley, K., Hyland, C., Zimmerman, A. R., & Lehmann, J. (2012). Corn growth and nitrogen nutrition after additions of biochars with varying properties to a temperate soil. Biology and Fertility of Soils, 48, 271-284. https://doi.org/10.1007/s00374-011-0624-7.
Rajkovich, S., Enders, A., Hanley, K., Hyland, C., Zimmerman, A.R., & Lehmann, J. (2011). Corn Growth and Nitrogen Nutrition after Additions of Biochars with Varying Properties to a Temperate Soil. Biology and Fertility of Soils, 48, 271-284. http://dx.doi.org/10.1007/s00374-011-0624-7.
Sajedi, N.A., Ardakani, M.R., Naderi, A., Madani, H., & Mashhadi Akbar Boojar, M. (2008). Effect of nutrition elements application on agronomical characters of hybrid maize (ksc.704) under water deficit stress at different growth stages. Iranian Journal of Agronomy and Plant Breeding, 4(1), 89-102. SID. https://sid.ir/paper/190385/en.
Shahbaz, M., & Ashraf, M. (2013). Improving Salinity Tolerance in Cereals. Critical Reviews in Plant Sciences, 32(4), 237–249. https://doi.org/10.1080/07352689.2013.758544.
Shahriyari, E. (2013). Investigating the spatial changes of some soil characteristics in the lands of Atabieh (Khuzestan province) using geostatistics, interpolation and GIS methods. Master’s Thesis, Shahid Chamran University of Ahvaz, Ahvaz. (In Persian).
Singh, M., & Malhotra, P.K. (1976). Selenium availability in berseem (Trifolium alexandrinum) as affected by selenium and phosphorus application. Plant and Soil, 44, 261-266. https://doi.org/10.1007/BF00016977.
Singla, A., Dubey, S.K., Singh, A., & Inubushi, K. (2014). Effect of biogas digested slurry-based biochar on methane flux and methanogenic archaeal diversity in paddy soil. Agriculture, Ecosystems & Environment, 197, 278-287. https://doi.org/10.1016/j.agee.2014.08.010.
Song, W., & Guo, M. (2012). Quality variations of poultry litter biochar generated at different pyrolysis temperatures. Journal of Analytical and Applied Pyrolysis, 94, 138-145. https://doi.org/10.1016/j.jaap.2011.11.018.
Taghavimehr, J. (2015). Effect of biochar on soil microbial communities, nutrient availability, and greenhouse gases in short rotation coppice systems of central Alberta. Master’s Thesis, University of Alberta, Edmonton. https://doi.org/10.7939/R32Z1311P.
Tenic, E., Rishikesh G., & Amit, D. (2020) Biochar-A Panacea for Agriculture or Just Carbon? Horticulturae, 6(3), 37. https://doi.org/10.3390/horticulturae6030037.
Thies, J.E., & Rillig, M.C. (2012). Characteristics of biochar: biological properties. Biochar for Environmental Management, 117-138. Routledge.
Ullah, S., Dahlawi, S., Naeem, A., Rangel, Z. & Naidu, R. (2018). Biochar application for the remediation of salt-affected soils: challenges and opportunities. Science of the Total Environment. 625. https://doi.org/10.1016/j.scitotenv.2017.12.257.
US Salinity Laboratory Staff. (1954). Diagnosis and improvement of saline and alkali soils. US Department of Agriculture Handbook 60, Washington, DC.
van Dijk, G., Lamers, L. P., Loeb, R., Westendorp, P. J., Kuiperij, R., van Kleef, H. H., ... & Smolders, A. J. (2019). Salinization lowers nutrient availability in formerly brackish freshwater wetlands; unexpected results from a long-term field experiment. Biogeochemistry, 143, 67-83. https://doi.org/10.1007/s10533-019-00549-6.
Verheijen, F., Jeffery, S., Bastos, A.C., Van Der Velde, M., & Diafas, I. (2010). Biochar application to soils: a critical scientific review of effects on soil properties processes and functions. Processes and Functions. EUR 24099 EN. Luxembourg (Luxembourg): European Commission. JRC55799.
Wakeel, A. (2013). Potassium–sodium interactions in soil and plant under saline‐sodic conditions. Journal of Plant Nutrition and Soil Science, 176(3), 344-354. https://doi.org/10.1002/jpln.201200417.
Walkley, A.J., & Black, I.A. (1934). Estimation of soil organic carbon by the chromic acid titration method. Soil Science, 37, 29-38.
Wang, J., & Wang Sh. (2019). Preparation, modification and environmental application of biochar: A review. Journal of Cleaner Production, 227, 1002-1022. https://doi.org/10.1016/j.jclepro.2019.04.282.
Xu, G., Zhang, Y., Sun, J., & Shao, H. (2016). Negative interactive effects between biochar and phosphorus fertilization on phosphorus availability and plant yield in saline sodic soil. Science of the Total Environment, 568, 910-915. https://doi.org/10.1016/j.scitotenv.2016.06.079.
Xue, T., Hartikainen, H. & Piironen, V. (2001). Antioxidative and growth-promoting effect of selenium on senescing lettuce. Plant and Soil, 237, 55–61. https://doi.org/10.1023/A:1013369804867.
Yanardag, İ. H., Yanardağ, A. B., Mermut, A. R., & Cano, A. F. (2022). Carbon storage potential and its distributions in the particle size fractions in harran plain, Turkey. Journal of Agricultural Sciences, 28(3), 501-510. https://doi.org/10.15832/ankutbd.907173.
Yuan, J.H., & Xu, R.K. (2010). Effects of rice-hull-based biochar regulating acidity of red soil and yellow brown soil. Journal of Ecology and Rural Environment, 26(5), 472-476. https://doi.org/10.5555/20103316564.
Zhang, H., Liao, W., Zhou, X., Shao, J., Chen, Y., Zhang, S., & Chen, H. (2022). Co-effect of pyrolysis temperature and potassium phosphate impregnation on characteristics, stability, and adsorption mechanism of phosphorus-enriched biochar. Bioresource Technology, 344 (B), 126273. https://doi.org/10.1016/j.biortech.2021.126273.
Zhao, R., Coles, N., Kong, Zh., & Wu, J. (2015). Effects of aged and fresh biochars on soil acidity under different incubation conditions. Soil and Tillage Research, 146 (B), 133-138. https://doi.org/10.1016/j.still.2014.10.014.