تأثیر ریزوباکترهای محرک رشد گیاه (Enterobacter cloacae) بر جذب و کارایی جذب پتاسیم در گیاه نیشکر (Saccharum officinarum L.)

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانش آموخته دکترا، گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه شهید چمران، اهواز، ایران

2 استاد، گروه علوم و مهندسی خاک, دانشکده کشاورزی, دانشگاه شهید چمران، اهواز، ایران

3 دانشیار، گروه علوم و مهندسی خاک, دانشکده کشاورزی, دانشگاه شهید چمران، اهواز، ایران

چکیده

کشت متراکم و پیوسته نیشکر بدون مصرف کود پتاسیمی، منجر به کاهش منابع پتاسیم در خاک شده است. به منظور بررسی کارایی جذب پتاسیم در دو مدیریت کود دهی، مشابه با کشت جدید نیشکر (پلنت) و سنین بازرویی (راتون) و نیز تأثیر ریزوباکترهای محرک رشد گیاه بر کارایی جذب پتاسیم، یک آزمایش گلدانی با سه تکرار در شرایط گلخانه انجام شد. تیمارها شامل ترکیب دو سطح کود فسفر (شامل صفر (P0) و 200 کیلوگرم در هکتار (P200)) و سه سطح ریزوباکتر (شامل شاهد (B0)، انتروباکتر سویه R13 (B13) و انتروباکتر سویه R33 (B33)) بودند. خصوصیات مورفولوژیکی اندام هوایی و ریشه و تغییرات پتاسیم در ریزوسفر در سه زمان برداشت 60، 95 و 140 روز پس از کشت بررسی شدند. در شرایط مشابه با کشت نیشکر، بیشترین مقدار جریان ورودی (اینفلاکس) و جذب پتاسیم در تیمارهای P200B13 و P200B0 مشاهده شد در صورتی که بیشترین طول ریشه در تیمار P200B33 مشاهده شد. این در حالی است که در شرایط مشابه با بازرویی نیشکر، بدون مصرف کود شیمیایی، بیشترین مقدار جریان ورودی و جذب پتاسیم در تیمار P0B33 مشاهده شد. در فاصله دو برداشت آخر، جریان ورودی پتاسیم در تیمار شاهد (P0B0) کاهش شدیدی نشان داد که در تیمارهای تلقیح شده، کاهش جریان ورودی پتاسیم مشاهده نشد. بنابراین در شرایط مشابه کشت نیشکر، استفاده از ریزوباکتر سویه R13 سبب بهبود کارایی جذب پتاسیم توسط گیاه نیشکر شد در حالی که در شرایط مشابه با سنین بازرویی، استفاده از ریزوباکترها به‌ویژه R33 توانست جذب و کارایی جذب پتاسیم را افزایش دهد. همچنین نتایج نشان داد که جریان ورودی پتاسیم، مکانیزم اصلی جذب پتاسیم به‌ویژه در استفاده از ریزوباکترها در گیاه نیشکر بود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of Plant Growth-Promoting Rhizobacteria (Enterobacter cloacae) on Uptake and Uptake Efficiency of Potassium in Sugarcane (Saccharum officinarum L.)

نویسندگان [English]

  • Saeed Safirzadeh 1
  • Mostafa Chorom 2
  • Naeimeh Enayatizamir 3
1 Ph.D of Soil Science, Department of Soil Science, Faculty of Agriculture, Sahid Chamran University, Ahvaz, Iran.
2 Professor, Department of Soil Science, Faculty of Agriculture, Shahid Chamran University, Ahvaz, Iran.
3 Associate professor, Department of Soil Science, Faculty of Agriculture, Shahid Chamran University, Ahvaz, Iran.
چکیده [English]

Intensive cropping of sugarcane and no application of potassium (K) fertilizer resulted a reduction of K pools in the soil. In order to evaluate the potassium uptake efficiency in two different fertilizer managements, new plantation of sugarcane and ratoon, and to investigate the effect of indigenous plant growth-promoting rhizobacteria on potassium uptake, a pot experiment with three replications was carried out in greenhouse condition. Treatments were the combination of two P rates (including: blank (P0) and 200 kg/ha (P200)), and two plant growth-promoting rhizobacteria strains (including: Enterobacter cloacae R13 (B13) and Enterobacter cloacae R33 (B33)). The morphological characteristics of sugarcane and root and potassium in the rhizosphere were evaluated at three harvesting times (60, 95 and 140 days after planting (DAP)). In management similar to plantation of sugarcane, higher K uptake and influx were found in P200B0 and P200B13 treatments, while the highest root length was observed in P200B33 treatment. In management like to ratoon, the greatest K uptake and influx were observed in P0B33 treatment. At the second harvesting period (between 95 and 140 DAP), K influx decreased severely in P0R0 treatment in comparison with the first harvesting period, while it was not occurred in the inoculated treatments. Therefore, in new sugarcane plantation condition, Enterobacter cloacae R13 improved K uptake efficiency, while in ratoon stage, Enterobacter cloacae R33 abled to increase uptake and uptake efficiency of K. Also, the result showed that the influx was the main mechanism of K uptake in sugarcane in inoculated treatments.

کلیدواژه‌ها [English]

  • Potassium uptake
  • Influx
  • Enterobacter cloacae
  • Root length
  • Sugarcane

Bagyalakshmi, B., Ponmurugan, P., and Marimuthu, S. (2012). Influence of potassium solubilizing bacteria on crop productivity and quality of tea (Camellia sinensis). African Journal of Agricultural Research, 7(30), 4250-4259.

Bakhshandeh, E., Pirdashti, H., and Shahsavarpour Lendeh, K. (2017). Phosphate and potassium-solubilizing bacteria effect on the growth of rice. Ecological Engineering, 103, 164-169.

Baranimotlagh, M., and Savaghebi Firozabadi, G. (2005). Investigation of potassium discharge from Sugar cultivated soils of Khuzestan. Iranian Journal of Agricultural Science, 36(5), 1185-1196. (In Farsi)

Basak, B. B., and Biswas, D. R. (2009). Influence of potassium solubilizing microorganism (Bacillus mucilaginosus) and waste mica on potassium uptake dynamics by sudan grass (Sorghum vulgare Pers.) grown under two Alfisols. Plant and Soil, 317, 235–255.

Bouyoucos, G. J. (1962). Hydrometer method improved for making particle size analyses of soils. Agronomy Journal, 54, 464–465.

El-Tilib, M. A., Elnasikh, M. H., and Elamin, E. A. (2004). Phosphorus and potassium fertilization effects on growth attributes and yield of two sugarcane varieties grown on three soil series. Journal of Plant Nutrition, 27(4), 663-669.

Etesami, H., Emami, S., and Alikhani, H. A. (2017). Potassium solubilizing bacteria (KSB): Mechanisms, promotion of plant growth, and future prospects - a review. Journal of Soil Science and Plant Nutrition, 17(4), 897-911.

Filho, J. O. (1985). Potassium nutrition of sugarcane. In: Potassium in agriculture. (Ed. Munson, R.D.). American Society of Agronomy, Crop Science Society of America, Soil Science Society of America, Madison, 1045-1062.

Flores, R. A., de Mello Prado, R., Pancelli, M. A., Almeida, H. J., Moda, L. R., Nogueira Borges, B. M. M., and de Souza Junior, J. P. (2014). Potassium nutrition in the first and second ratoon sugarcane grown in an Oxisol by a conservationist system. Chilean Journal of Agricultural Research, 74(1), 83-88.

Föhse, D., Claassen, N., and Jungk, A. (1991). Phosphorus efficiency of plants, II. Significance of root radius, root hairs and cation-anion balance for phosphorus influx in seven plant species. Plant and Soil, 132, 261-272.

Gupta, G., Parihar, S. S., Ahirwar, N. K., Snehi, S. K., and Singh, V. (2015). Plant growth promoting rhizobacteria (PGPR): current and future prospects for development of sustainable agriculture. Journal of Microbial & Biochemical Technology, 7, 96-102.

Helmke, P. A., and Sparks, D. L. (1996). Lithium, Sodium, Potassium, Rubidium and Cesium. In D. L. Sparks (Ed.), Methods of Soil Analysis (Part 3). (pp. 551-574). Soil Science Society of America Publishing: Madison, Wisconsin, USA.

Jafari, S., and Baghernejad, M. (2007). Effects of wetting and drying and culture systems on potassium fixation in some soils and clays of Khuzestan. Journal of Agricultural Science and Technology, 41, 75-89. (In Farsi)

Jafarnejady, A. (2013). Study of the reaction of sugarcane (Saccharum officinarum L.) to different of potassium fertilizers. Journal of Plant Physiology, 19, 61-71. (In Farsi)

Khorassani, R. (2010). Phosphorus uptake efficiency in corn, sugar beet and groundnut. Journal of water and soil, 24(1), 180-188. (In Farsi)

Lamizadeh, E., Enayatizamir, N., and Motamedi, H. (2016). Isolation and identification of plant growth-promoting rhizobacteria (PGPR) from the rhizosphere of sugarcane in saline and non-saline soil. International Journal of Current Microbiology and Applied Sciences, 5, 1072-1083.

Loeppert, H. L., and Suarez, D. L. (1996). Carbonate and gypsum. Methods of Soil Analysis. In D. L. Sparks (Ed.), Methods of Soil Analysis (Part 3). (pp. 437-474). Soil Science Society of America Publishing: Madison, Wisconsin, USA.

Meena, V. S., Maurya, B. R., Verma, J. P., Aeron, A., Kumar, A., Kim, K., and Bajpai, V. K. (2015). Potassium solubilizing rhizobacteria (KSR): Isolation, identification, and K-release dynamics from waste mica. Ecological Engineering, 81, 340-347.

Miller, R. O. (1998). Determination of dry matter content of plant tissue: gravimetric moisture. Handbook of Methods for Plant Analysis. CRC Press.

Nelson, D. W., and Sommers, L. E. (1996). Total carbon, organic carbon and organic matter. In D. L. Sparks (Ed.), Methods of Soil Analysis (Part 3). (pp. 961-1010). Soil Science Society of America Publishing: Madison, Wisconsin, USA.

Olsen, S. R., Cole, C. V., Watanabe, E. S., and Dean, L. A. (1954). Estimation of available phosphorus in soils by extraction with sodium bicarbonate. United States Department of Agriculture Circular, 939, 1-18.

Ostonen, I., Pu¨ ttsepp, U¨., Biel, C., Alberton, O., Bakker, M. R., Lo˜ hmus, K., Majid, H., Metcalfe, D., Olsthoorn, A. F. M., Pronk, A., Vanguelova, E., Weih, M., and Brunner, I. (2007). Specific root length as an indicator of environmental change. Plant Biosystems, 141(3), 426.442.

Prajapati, K., and Modi, H. (2016). Growth promoting effect of potassium solubilizing Enterobacter hormaechei (KSB-8) on cucumber (Cucumis sativus) under hydroponic conditions. International Journal of Advanced Research in Biological Sciences, 3(5), 168-173.

Samadi, A. (2012). Impact of continuous sugar beet cropping on potassium quantity-intensity parameters in calcareous soils. Journal of Plant Nutrition, 35, 1154–1167.

Samal, D., Kovar, J. L., Steingrobe, B., Sadana, U. S., Bhadoria, P. S., and Claassen, N. (2010). Potassium uptake efficiency and dynamics in the rhizosphere of maize (Zea mays L.), wheat (Triticum aestivum L.), and sugar beet (Beta vulgaris L.) evaluated with a mechanistic model. Plant and Soil, 332, 105–121.  

Sangeeth, K. P., Bhai, S. R., and Srinivasan, V. (2012). Paenibacillus glucanolyticus, a promising potassium solubilizing bacterium isolated from black pepper (Piper nigrum L.) rhizosphere. Journal of Spices and Aromatic Crops, 21(2), 118–124.

Sumner, M. E., and Miller, W. P. (1996). Cation exchange capacity and exchange coefficients. In D. L. Sparks (Ed.), Methods of Soil Analysis (Part 3). (pp. 1201-1229). Soil Science Society of America Publishing: Madison, Wisconsin, USA.

Tennant, D. (1975). A test of a modified line intercepts method of estimating root length. Journal of Ecology, 63, 995-1001.

Wood, R. A., and Meyer, J. H. (1986). Factors affecting potassium nutrition of sugarcane in south africa. Proceedings of the South African Sugar Technologists' Association, 198-204.