Rice (Oryza sativa L.) is one of the Iran's most strategic crops with water shortage as the most important abiotic stress limiting its yield. In the present research the effects of: variety, water regime, Nitrogen (N) and Phosphorus (P) levels on N, P and K uptake were studied under greenhouse conditions. The experiment design was a factorial one based upon a randomized complete blocks of three replications. The factors included two varieties of rice (Hashemi and Ali Kazemi), two water regimes (flooded vs. periodic saturation), three nitrogen levels as urea (0, 75, 150 mg kg^-1) and three P levels as mono calcium phosphate (0, 25, 50 mg kg^-1). After 90 days past of growth period, plants were harvested, and total content as well as concentration of N, P, and K in shoot and root determined. The results revealed that K concentrations in shoot and root, K content in shoot and N translocation factor in Ali Kazemi were greater than those in Hashemi variety. But P concentration in root, P translocation factor and P content of the root (in Hashemi) became higher that those in Ali Kazemi variety. P concentration in shoot and root, shoot K concentration, N content of shoot, P and K contents of the shoot and root (under flooded conditions) were significantly (P<0.01) higher that those under periodic saturation conditions. Interaction between N and P led to significant effects on N and K concentrations in shoot and root, P concentration in root, N and P translocation factors, and as well on N, P and K contents of the shoot and root.