The Effect of Nitrate, Salinity, Pollutant Concentration and Cell Mass on BTEX Degradation in Microaerophilic Environment

Document Type : Research Paper

Authors

1 PhD Student, Irrigation and Reclamation Engineering Department, Faculty of Agricultural Engineering and Technology, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Professor, Irrigation and Reclamation Engineering Department, Faculty of Agricultural Engineering and Technology, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Associate Professor, Irrigation and Reclamation Engineering Department, Faculty of Agricultural Engineering and Technology, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

4 Associate Professor, Department of Soil Science, Faculty of Agricultural Engineering and Technology, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Abstract

Despite of numerous studies on BTEX biodegradation, a few researches has been conducted to optimize the environmental conditions for biodegradation of this pollutant considering the effective factors. The objective of this study was to investigate the effect of environmental factors such as nitrate, salinity and cell mass (the isolated bacterium from the soil) on BTEX degradation and to optimize the environmental conditions for biodegradation. In order to identify the appropriate microorganism for BTEX degradation, isolation of the bacterium from the oil contaminated soil was performed firstly. Then the nitrate, salinity, cell mass and BTEX concentrations were considered as independent variables to optimize BTEX degradation conditions by the isolated bacterium. Finally, a quadratic polynomial mathematical model was suggested by the Design Expert software (R2=0.85). This research showed that the isolated bacterium is able to degrade BTEX and the proper condition for degradation can be achieved by applying the above-mentioned model. Results showed that the effect of BTEX and Nitrate concentration on BTEX degradation is significant. So that increasing BTEX concentration to the extent of 200 ppm and decreasing nitrate concentration to the extent of 400 ppm reduced BTEX degradation to 4.2% and 9%, respectively.

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References

 Atlas, R., Hazen. T.C.(2011). Oil Biodegradation and Bioremediation; A Tale of the Two Worst Spills in U.S. History. Environmental Science& Technology. 45(16), 6709-6715
Corseuil, H.X., Gomez, D. E., Schambeck, C. M., Ramos, D. T., Alvarez. P.J.J. (2015). Nitrate addition to groundwater impacted by ethanol-blended fuel accelerates ethanol removal and mitigates the associated metabolic flux dilution and inhibition of BTEX biodegradation. Journal of Contaminant Hydrology. 174, 1–9
Coates, J.D., Chakraborty, R., Lack, J.G., Achenbach, L.A. (2001). Anaerobic benzene oxidation coupled to nitrate reduction in pure culture by two strains of Dechloromonas. Nature 411: 1039–1043.
Cunningham, J.A., Rahme, H., Hopkins, G.D., Lebron, C. and Reinhard, M. (2001). Enhanced in situ bioremediation of BTEX-contaminated groundwater by combined injection of nitrate and sulfate. Environmental Science & Technology 35(8), 1663-1670
Dou. J., Liu. X., Hu. Z., Deng. D.( 2008). Anaerobic BTEX biodegradation linked to nitrate and sulfate reduction, J. Hazard. Mater. 151, 720–729.
Fathepure, B. (2014). Recent studies in microbial degradation of petroleum hydrocarbons in hypersaline environments. Review article.: Frontiers in Microbiology. doi: 10.3389/fmicb.00173
Ferreira, S.C., Bruns, R. Ferreira, H., Matos, G., David, J., Brandao, G. (2007). Box-Behnken design: An alternative for the optimization of analytical methods. Analytica Chimica Acta. 597(2), 179-86.
Gandolfi, I., Sicolo, M., Franzetti, A., Fontanarosa, E., Santagostino, A., Bestetti, G. (2010). Influence of compost amendment on microbial community and ecotoxicity of hydrocarbon-contaminated soils. Bio resource. Technol. 101, 568–575.
Jin, H.M., Kim, J.M., Lee, H.J., Madsen, E.L., Jeon, C.O.M. (2012). Alteromonas as a key agent of polycyclic aromatic hydrocarbon biodegradation in crude oil contaminated coastal sediment. Environ. Sci. Technol. 46, 7731–7740
Jeon, C.O., Park, W., Padmanabhan, P., DeRito, C., Snape, J.R., Madsen, E.L. (2003). Discovery of a previously undescribed bacterium with distinctive dioxygenase that is responsible for in situ biodegradation in contaminated sediment. Proc. Natl. Acad. Sci. USA 100, 13591–13596.
Khajeh, M., Mosavi Zadeh, F. (2012). Response Surface Modeling of Ultrasound- Assisted Dispersive Liquid- Liquid Microextraction for Determination of Benzene, Toluene and Xylene in water Sample. Bull Environ Contam Toxicol. 89, 38-43.
Knezevich, V., Koren, O., Ron, E.Z., Rosenberg, E. (2006). Petroleum bioremediation in seawater using guano as the fertilizer. Biorem. J. 10, 83–91.
Kim, J.M., Jeon, C.O. (2009). Isolation and characterization of a new benzene, toluene, and ethyl benzene degrading bacterium, Acinetobacter sp. B113. Curr. Microbiol.58, 70–75.
Maxwell, C.R., Baqai, H.A. (1995). Remediation of petroleum hydrocarbons by inoculation with laboratory-cultured microorganisms. In: Hinchee RE, Fredrickson J, Alleman BC Bioaugmentation for site remediation. Battelle Press, Columbus.
Mi Jin, H., Choi, E. J., Jeon, C.O. (2013). Isolation of a BTEX-degrading bacterium, Janibacter sp. SB2, from a sea-tidal flat and optimization of biodegradation conditions. Bioresource Technology. 145, 57–64.
Myers, R.H., Montgomery, D.C., Anderson-Cook, C.M. (2009). Response Surface Methodology: Process and Product Optimization Using Designed Experiments, third ed. New York.
Newell, C.J., McLeod, R.K., Gonzales, J.R., and Wilson, J.T. (1996). Bioscreen Natural Attenuation Decision Support System, User’ s Manual-Version 1.3, National Risk Management Research Laboratory Office of Research and Development U.S. Environmental Protection Agency, Cincinnati, Ohio, EPA/600/R-96/087.
Ruiz, M., Pasadakis, N., Kalogerakis, N. (2006). Bioremediation and toxicity determination of natural seawater polluted with weathered crude oil by salt tolerant consortia in a SBR. Mar. Pollut. Bull. 52, 1490–1493.
Saeki, H., Sasaki, M., Komatsu, K., Miura, A., Matsuda, H. (2009). Oil spill remediation by using the remediation agent JE1058BS that contains a biosurfactant produced by Gordonia sp. strain JE-1058. Bioresour. Technol. 100, 572–577.
Shahriari, M.H. (2013). Investigation of bioavailability and biodegradation kinetics of phenantherene in saline soils. PhD Thesis, University of Tehran.
Shim, H., Hwang,B., Lee,S. S., and Kong,S.H. (2005). Kinetics of BTEX biodegradation by a coculture of Pseudomonas putida and Pseudomonas fluorescens under hypoxic conditions. Biodegradation 16, 319–327.
Shinoda, Y., Sakai, Y., Uenishi, H., Uchihashi, Y., Hiraishi, A., Yukawa, H., Yurimoto, H., Kato, N. (2004). Aerobic and anaerobic toluene degradation by a newly isolated denitrifying bacterium, Thauera sp. strain DNT-1. Appl. Environ. Microbiol. 70, 1385–1392.
Sunita, J. (2017). Microbial degradation of petroleum hydrocarbons. Bioresource Technology. 223, 277–286.
Ucankus, T. (2005). Modeling natural attenuation of petroleum hydrocarbons (BTEX) in heterogeneous aquifers.MSc. thesis, Middle East Technical University.
Vogt, C., Kleinsteuber, S., Richnow H.H. (2011). Anearobic benzene degradation by bacteria. Microbial Biotechnology 4(6), 710–724
Iranian Journal of Soil and Water Research
Volume 50, Issue 2
March and April 2019
Pages 401-409

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