Quantitative and Qualitative Evaluation of Auxin (IAA) Production Potential of Cyanobacteria, Isolated from Guilan Paddy Fields

Document Type : Research Paper

Authors

1 Ph.D. Candidate, of soil biology and biotechnology, Tabriz University

2 Professor, soil biology, department of soil sciences, Tabriz University

3 Professor, biotechnology, Agriculture and Natural Resources, University of Sari and Genetic and Biotechnology Institute of Tabarestan, Mazandaran

4 Associate professor, Department of Biology, Research Institute of Applied Sciences, Shahid Beheshti University, Tehran

Abstract

Cyanobacteria represent a less investigated group of prokaryotes, in terms of their effect on plant growth, especially in relation with the production of phytohormones. The present research was aimed at evaluating Indole Acetic Acid (IAA) production potential of cyanobacteria strains isolated from Guilan paddy fields through the  two quantitative and qualitative methods, their potential being determined in terms of rice seed germination indices. The results obtained indicated that some cyanobacteria isolates could produce auxin hormone IAA.GGuCy-34 and GGuCy-42 isolates respectively produced 14.98 and 10.83 [µg IAA/(ml. Chl. a)] in no L-Trp treatment, GGuCy-34, GGuCy-15 and GGuCy-42 isolates respectively produced 23.7, 17.46 and 15.81 [µg IAA/(ml. Chl. a)] in 100 (mg L-Trp/ml) treatment and GGuCy-15 and GGuCy-16 isolates respectively produced 29.16 and 21.61 [µg IAA/(ml. Chl. a)] in 500 (mg L-Trp/ml) treatment. The results finally revealed that IAA production is highly correlated with the type of isolate and as well with the culture medium. Germination energy and germination rates increased in the cases of GGuCy-25, GGuCy-42, GGuCy-41, GGuCy-26 and GGuCy-50 isolates, and while dry radical weight as well as dry plumule weight increased in the cases of GGuCy-42, GGuCy-50, GGuCy-25 isolates. 

Keywords

Main Subjects

References

Agarwal, R. L. (2003). Seed technology. Publication Company Limited New Delhi, India. 550pp.
Ahmed, M., Stal, L. J., and Hasnain, S. (2010). Association of non-heterocystous cyanobacteria with crop plants. Plant and Soil. 336:363–375.
Arshad, M. and Frankenberger, W. T. (1998). Plant growth substances in the rhizosphere: microbial production and functions. Advanced Agronomy, 62: 46-151.
Asghar, H. N., Zahir, Z. A., and Arshad, M. (2004). Screeninig rhizobacteria for improving the growth, yield, and oil content of canola (Brassica napus L.). Australian Journal of Agricultural research. 55: 187-194.
Begum, Z. N. H., Mandal, R., and Islam, S. (2011). Effect of cyanobacterial biofertilizer on the growth and yield components of two HYV of rice. Journal of Algal Biomass and Utln., 2(1): 1-9.
Bric, J. M., Bostok, R. M., and Silverston, S. A. (1991). Rapid in situ assay for indoleacetic production by bacteria immobilized on a nitrocellulose membrance. Applied Environmental Microbiology, 57(2): 535-538.
Desikhachary, T. V. (1959). Cyanophyta. Indian Council of Agricultural Research Publishers pp. 565.
Glick, B. R. (1995). The enhancement of plant growth by free – living bacteria. Canadian Journal of Microbiology .41: 109 –117.
Johansson, C. and Bergman, B. (1994). Reconstitution of the symbiosis of Gunnera manicata Linden: cyanobacterial specificity. New Phytology. 126:643–652.
John, D. M., Whitton, B. A., and Brook, A. J. (2003). The freshwater algal flora of the British Isles, an identification guide to freshwater and terrestrial algae. Cambridge University Press.
Karthikeyan, N., Prasanna, L. R., and Kaushik, B. D. (2007). Evaluating the potential of plant growth promoting cyanobacteria as inoculants for wheat. European Journal of Soil and Biology. 43: 23-30.
Kaushik, B. D. (1987). Laboratory Methods for Blue-green Algae. Associated Publishing Company. Pp. 171.
Madigan, M. T., Martinko, J. M., Stahl, D. A., and Clark, D. P. (2012). Brock Biology of Microorganisms (13th ed).pp. 532-536. Publishing as Benjamin Cummings, San Francisco. Manufactured in the U.S.A.
Maguire, J. D. (1962). Speed of germination-aid in selection and evaluation for seedling emergence and vigour. Crop Science. 2: 176-177.
Mazhar, S. and Hasnain, S. (2011). Screening of native plant growth promoting cyanobacteria and their impact on Triticum aestivum var. Uqab 2000 growth. African Journal of Agricultural Research, 6(17):3988-3993.
Mishra, Y., Bhargava, P., Chaurasia, N., and Rai, L. C. (2009). Proteomic evaluation of the non-survival of Anabaena doliolum (Cyanophyta) at elevated temperatures. European Journal of  Phycology, 44(4): 551–565.
Porra, R. J., Thompson, W. A., and Kriedemann, P. E. (1989). Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents; verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimical and Biophysical Acta. 975:384–394.
Prasanna, R., Jaiswal, P., Nayak, S., Sood, A., and Kaushik, B. D. (2009). Cyanobacterial diversity in the rhizosphere of rice and its ecological significance. Indian Journal of  Microbiology, 49: 89-97.
Prasanna, R., Sharma, E., Sharma, P., Kumar, A., Kumar, R., Gupta, V., Pal, R. K., Shivay, Y. S., and Nain, L. (2013). Soil fertility and establishment potential of inoculated cyanobacteria in rice crop grown under non-flooded conditions. Paddy Water and Environment, 11:175–183.
Prescott, G. W. (1970). Algae of The Western Great Lakes Area. W.M.C. Brown Company Publishers. 977 pp.
Rodriguez, A. A., Stella, A. A., Storni, M. M., Zulpa, G., and Zaccaro, M. C. (2006). Effects of cyanobacterial extracellular products and gibberellic acid on salinity tolerance in Oryza sativa L. Saline System, 2: 7.
Saadatnia, H. and Riahi, H. (2009). Cyanobacteria from paddy fields in Iran as a biofertilizer in rice plants. Plant and Soil Environment, 55 (5): 207–212
Sergeeva, E., Liaimer, A., and Bergman, B. (2002). Evidence for production of the phytohormone indole-3-acetic acid by cyanobacteria. Planta. 215: 229–238.
Shrivastava, U. P. and Kumar, A. (2011). A simple and rapid plate assay for the screening of indole-3-acetic acid (IAA) producing microorganisms. International Journal of Applied Biology and Pharmaceutical Technology, 2(1): 120-124.
Soltani, N., Khavari-Nejad, R., Tabatabaie, M., Shokravi, S. H., and Valiente, E. F. (2006). Variation of Nitrogenase Activity, photosynthesis and pigmentation of cyanobacterium Fischerella ambigua strain FS18 under different irradiance and pH. World Journal of Microbiology and Biotechnology. 22 (6): 571-576.
Stanier, R. Y., Kunisawa, R., Mandal, M., and Cohen-Bazire, G. (1971). Purification and properties of unicellular blue green algae (Order: Chroococcales), Bacteriological Reviwe. 35: 171-305.
Szkop, M. and Bielawski, W. (2013). A simple method for simultaneous RP-HPLC determination of indolic compounds related to bacterial biosynthesis of indole-3-acetic acid. Antonie van Leeuwenhoek. 103:683–691.
Thajuddin, N. and Subramanian, G. (2005). Cyanobacterial biodiversity and potential applications in biotechnology. Current Science. 89: 47–57.
Tien, T. M., Gaskins, M. H., and Hubbell, O. H. (1979). Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet. Applied Environmental Microbiology. 37: 1016-1024.
Torres-Rubio, M. G., Astrid, S., Castillo, J., and Martiners, P. (2000). Isolation of Enterobacteria, Azotobacter sp. And Pseudomonas sp., producers of indole-3-acetic acid and Siderophores, from colombian rice rhizosphere. Revista Latinoamericana de Microbiologia. 42: 171-176.
Varalakshmi, P. and Malliga, P. (2012). Evidence for production of Indole-3-acetic acid from a fresh water cyanobacteria (Oscillatoria annae) on the growth of H. annus. International Journal of Scientific and Research Publications, 2(3):1-15.
Iranian Journal of Soil and Water Research
Volume 46, Issue 3 - Serial Number 3
October 2015
Pages 589-596

Files

Share

How to cite

Statistics