تأثیر کود ترکیبی فسفر و مواد آلی بر ویژگی‌های مورفولوژیکی و فیتوشیمیایی بادرشبو (Dracocephalum moldavica L.)

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه علوم خاک، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران

چکیده

تأمین عناصر غذایی به ویژه فسفر (P)، پارامترهای رشد و متابولیت‌‌های ثانویه را در گیاهان دارویی بهبود می‌‌بخشد. این پژوهش با هدف بررسی ویژگی‌‌های مورفولوژیکی، فیتوشیمیایی و میزان جذب عناصر فسفر، منیزیم و نیتروژن گیاه بادرشبو (Dracocephalum moldavica L.) در حضور چهار نمونه کود ترکیبی کندرها (OM0/P، OM25/P، OM50/P و OM75/P) در مقایسه با مونو پتاسیم فسفات (KH2PO4) و نمونه شاهد (بدون کود فسفره و ماده آلی) در خاک آهکی انجام شد. نتایج نشان داد حضور ماده آلی لئوناردیت در ترکیب کود فسفاته استرووایت موجب افزایش جذب عناصر فسفر، منیزیم و نیتروژن در گیاه در مقایسه با تیمار شاهد و KH2PO4 شد. بعلاوه ویژگی‌‌های موفولوژیکی (وزن خشک و تر، ارتفاع بوته، قطر ساقه، کلروفیل برگ و تعداد برگ) و ویژگی‌‌هایی شامل مقدار فسفر در بافت گیاهی، فنول، فلاونوئید و فعالیت آنتی اکسیدانتی FRAP و DPPH با افزایش مقدار ماده آلی در کود ترکیبی بیشتر شد و روند افزایش بدین صورت است: OM25/P < KH2PO4 < OM50/P < OM75/P < OM0/P. وزن خشک و غلظت فسفر گیاه بادرشبو در حضور OM75/P به ترتیب ۴۲ و ۷۸ درصد در مقایسه با تیمار شاهد افزایش یافت. میزان فلاونوئید گیاه به ترتیب ۳۳، ۴۷، ۷۱، ۱۰۰، و ۶۳ درصد در مقایسه با تیمار شاهد در حضور تیمارهای کودی شامل OM0/P، OM25/P، OM50/P و OM75/P و KH2PO4 افزایش یافت. بنابراین کاربرد کود ترکیبی فسفر و ماده آلی به عنوان کود فسفاته کندرها در خاک‌‌های آهکی موجب دسترسی گیاه به عناصر غذایی به ویژۀ فسفر و در نتیجه بهبود ویژگی‌‌های فیتوشیمیایی و مورفولوژیکی گیاه بادرشبو گردید.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of combined phosphorus-organic matter fertilizer on the morphological and phytochemical characteristics of Moldavian Balm (Dracocephalum moldavica L.)

نویسندگان [English]

  • Marziyeh Piri
  • Ebrahim Sepehr
  • mahrokh sharifmand
Department of Soil Science, Urmia University, Urmia, Iran
چکیده [English]

Nutrients supply, especially phosphorus (P) can improve the growth and the production of secondary metabolites in medicinal plants. This study aims to investigate the morphological, phytochemical characteristics and absorption of phosphorus, magnesium and nitrogen elements by the Moldavian balm (Dracocephalum moldavica L.) in the presence of slow-release combined fertilizer of P and organic matter (OM0/P، OM25/P، OM50/P and OM75/P) compared to mono-potassium phosphate (KH2PO4) and the control sample (without P-fertilizer) in a calcareous soil. The presence of leonardite in the struvite composition increased the uptake of P, magnesium and nitrogen elements by the plant compared to the control and KH2PO4 treatments. In addition, the morphological characteristics (dry and wet weight, plant height, stem diameter, chlorophyll and number of leaves) and characteristics including a value of P in plant, phenol, flavonoid and FRAP and DPPH antioxidant activity increased with increasing the amount of organic matter in combined fertilizer and the increasing trend was as follows: OM0/P < OM25/P <KH2PO4< OM50/P < OM75/P. The dry weight and value of P in Moldavian balm by application of OM75/P increased by 42 and 78%, respectively, compared to the check treatment. The flavonoid content of the plant increased by 33, 47, 71, 100, and 63%, respectively, compared to the control including OM0/P, OM25/P, OM50/P, OM75/P, and KH2PO4. Therefore, the application of combined fertilizer of P and organic matter as a slow-release-P fertilizer leading to plant availability to nutrients, especially P, as a result improve the phytochemical and morphological characteristics of Moldavian balm in calcareous soils.

کلیدواژه‌ها [English]

  • Calcareous soil
  • Struvite
  • Organic matter
  • Phytochemical compounds

The effect of combined fertilizer of phosphorus and organic matter on the morphological and phytochemical characteristics of Moldavian Balm (Dracocephalum moldavica L.)

 

EXTENDED ABSTRACT

 

Introduction

Phosphorus (P) is one of the essential nutrients in plants. Suppling of nutrients from different sources of fertilizers can change the growth and the production of secondary metabolites in medicinal plants. Recently, the recycle of P from secondary sources such as sewage, as slow-release-fertilizer have been noticed all over the world. The low solubility of P fertilizers in calcareous soils caused a limitation in the supply of plant phosphorus, and the presence of organic matter in the stage of its formation can increase the release of P from its composition. This study purposes to recycle of P from sewage sludge as struvite combined with different amount of organic matter and application of them as P fertilizers. The morphological, phytochemical characteristics and absorption of phosphorus, magnesium and nitrogen elements by the Moldavian Balm (Dracocephalum moldavica L.) investigated in the presence of four samples of slow-release combined fertilizer of phosphorus and organic matter (OM0/P، OM25/P، OM50/P and OM75/P) compared to mono-potassium phosphate (KH2PO4) and the check sample (without using phosphate fertilizer) in a calcareous soil.

Methods

      The Stuttgart Process method was used to make the combined fertilizer of P and organic matter (OM0/P، OM25/P، OM50/P and OM75/P). Briefly, fertilizers were prepared in molar ratios of NH4+/Mg2+/PO43- 1:1:1 at a pH of 8.5. The experiment was conducted in the form of a completely randomized design in a calcareous soil to investigate the morphological and phytochemical characteristics of Moldavian balm. The morphological characteristics of plants including dry/wet weight, plant height, stem diameter, leaf chlorophyll and number of leaves were measured. Also, the phytochemical characteristics (flavonoid, phenol, DPPH, FRAP) and the values of P, Mg and N nutrients were measured in Moldavian balm in the presence of P-fertilizers treatments.

Results

The results of this study showed that the use of combined fertilizers (OM0/P، OM25/P، OM50/P and OM75/P) and KH2PO4 resulted in a significantly increase in the morphological characteristics (dry and wet weight, plant height, stem diameter, chlorophyll and number of leaves) and phytochemical characteristics including phenol, flavonoid and FRAP and DPPH antioxidant activity especially with the high amount of organic matter in combined fertilizer and the increasing trend was as follows: OM0/P < OM25/P <KH2PO4< OM50/P < OM75/P.

Conclusion

The use of combined phosphorus/organic matter fertilizers, especially with a high amount of organic matter, are effective to increase secondary metabolites (phenols and flavonoids), antioxidant activities, and the growth of Moldavian balm in calcareous soils. Further research is also needed to determine the economics of using OM/P components under large scale cultivation of Moldavian balm and different plants in various conditions.

مراجع

Adam, C., Peplinski, B., Michaelis, M., Kley, G., & Simon, F. (2009). Thermochemical treatment of sewage sludge ashes for phosphorus recovery. Waste Management, 29, 1122–1128.
Ahmadi, F., Samadi, A., & Rahimi, A. (2020). Improving growth properties and phytochemical compounds of Echinacea purpurea (L.) medicinal plant using novel nitrogen slow release fertilizer under greenhouse conditions. Scientific Reports, 10, 13842. doi: 10.1038/s41598-020-70949-4.
Aminifard, M. H., & Amiri, M. B. (2021). Growth and yield of saffron (Crocus sativus L.) affected by different levels of fulvic acid and cow manure in the second growing season, Journal of Horticulture and Postharvest Research, 4(3), 57-67.
Bakshi, S., Banik, C., Laird, D., Smith, R., & Brown, R. (2021). Enhancing Biochar as Scaffolding for Slow Release of Nitrogen Fertilizer. ACS Sustainable Chemistry & Engineerin, 9 (24), 8222–8231. https://doi.org/10.1021/acssuschemeng.1c02267.
Bertrand, I. R., Holloway, E., Armstrong, R. D., & McLaughlin, M. J. (2003). Chemical Characteristics of phosphorus in alkaline soils from southern Australia. Aust. Journal of Soil Research, 41, 61-76.
Emami, A. (1996). Methods of plant analysis. Soil and Water Research Institute, technical publication No. 982, Tehran, Iran, 128p. (In Persian).
Farahmnad. S., Shiralizadeh, S., Talebian, M. T., Bagheri-Hariri, S., Arbab, M., Basirghafouri, H., Saeedi, M., Sedaghat, M., & Mirzababai, H. (2014). Nebulized fentanyl vs intravenous morphine for ED patients with acute limb pain: a randomized clinical trial. American Journal of Emergency Medicine, 32(9), 1011-5. doi: 10.1016/j.ajem.2014.05.051. 
Gee, G. W., & Bauder, J. W. (1986). Particle-size analysis.  2nd ed.  Methods  of  soil  analysis  Part  1  Physical  and miner-alogical  methods,  383–411. 2nd ed.  American Society of Agronomy/Soil Science Society of America.
Grotz, N., & Guerinot, M. L. (2002). Limiting nutrients: An old problem with new solutions. Plant Biology, 5, 158-163.
Guney, E., Menche, J., Vidal, M. et al. (2016). Network-based in silico drug efficacy screening. Nature Communications, 7, 10331. https://doi.org/10.1038/ncomms10331.
Gürgan Eser, M., & Adiloglu, S. (2020). The correlation of some nutrient elements and antibacterial activity of the basil (Ocimum basilicum). Tekirdag Ziraat Fakültesi Derg. 17, 381–391. https://doi.org/10.33462/jotaf.699792.
Herzberger, V., Bäz, E., Kunze, M., Markfeld-Erol, F., & Juhasz-Böss, I. (2022). Exercise during pregnancy—effects on birth weight and on the risks of gestational diabetes and preterm delivery. Deutsches Ärzteblatt International, 119, 793–7. DOI: 10.3238/arztebl.m2022.0305.
Hu, Y., Yuan, B., Cheng, F., & Hu, X. (2019). NaOH etching and resin pre-coating treatments for stronger adhesive bonding between CFRP and aluminium alloy. Compos. Bachelor of Engineering, 178, 107478 https://doi.org/10.1016/j.compositesb.2019.107478.
Jahandideh, A., Barani Motlagh, M., Dordipur, E., & Ghorbani Nasrabad, R. (2020). The Effect of Humic Acid on the Availability of Phosphorus Fertilizer and some Physiological Traits of Rapeseed (Canola). Journal of Water and Soil, 33(6), 837-884. DOI: 10.22067/jsw.v33i6.77449 (In Persian).
Jama-Rodzenska, A., Chohura, P., Gałka, B., Szuba-Trznadel, A., Falkiewicz, A., & Białkowska, M. (2022).  Effect  of different  doses  of  phosgreen  fertilization  on  chlorophyll,  K,  and  Ca  content  in  butter  head  lettuce  (Lactuca  sativa  L.) grown in peat substrate. Agriculture, 12(6), 788.doi:10.3390/agriculture12060788.
Joshi, R. (2014). Chemical composition and antimicrobial activity of the essential oil of Ocimum basilicum L. (sweet basil) from Western Ghats of North West Karnataka, India. Ancient Science of Life, 33, 149. https://doi.org/10.4103/0257-7941.144618.
Krueger, J., & Mueller, R. A. (2000). Unskilled, unaware, or both? The better-than-average heuristic and statistical regression predict errors in estimates of own performance. Journal of Personality and Social Psychology, 82(2), 180-8.
Li, M., Zhang, H., Sun, H., Mohammed, A., Liu, Y., & Lu, Q. (2022).  Effect  of  phosphate  and  ammonium  concentrations,  total  suspended  solids  and  alkalinity  on  lignin-induced  struvite  precipitation.  Scientific Reports, 12 (1), 2901.  doi:  10.1038/s41598-022-06930-0.
Mazaraie, A., Mousavi-Nik, S. M., & Leila, F. (2018). Assessments of phenolic, flavonoid and antioxidant   activity   of aqueous, alcoholic, methanol and acetone extracts of thirteen medicinal plants. Nova Biologica Reperta, 4(4), 299-309.
Mehra, P., Pandey, B. K. & Giri, J. (2015). Genome-wide DNA polymorphisms in low Phosphate tolerant and sensitive rice genotypes. Scientific Reports, 5, 13090.
Meyer, C., Preyl, V., Steinmetz H., Maier W., Mohn RE. & Schönberger H. (2019). The Stuttgart Process (Germany). In: Ohtake, H., Tsuneda, S. (Eds) Phosphorus Recovery and Recycling. Springer, Singapore. https://doi.org/10.1007/978-981-10-8031-9_19.
Mrabti, H. N., El Hachlafi, N., Al-Mijalli, S. H., Jeddi, M., Elbouzidi, A., et al. (2023). Phytochemical Profile, Assessment of Antimicrobial and Antioxidant properties of Essential Oils of Artemisia herba-alba Asso., and Artemisia dracunculus L.: Experimental and Computational Approaches. Journal of Molecular Structure, DOI:10.1016/j.molstruc.2023.136479.
Olsen, S. R., & Sommers, L. E. (1982).  Phosphorus. In Methods of soil analysis.  Part 2. Chemical and microbiological properties, 2nd ed., eds.  A.  L.  Page, R.  H.  Miller, and D.  R.  Keeney, 403–30.  Agron. Monogr. 9; Madison, WI: ASA and SSSA.
Piri, M., & Sepehr, E. (2022).  Phosphorus  recovery  from  domestic  sewage  sludge  in  the  presence  of  waste  grape pruning  biochar.  Global Journal of Environmental Science and Management, 8(4), 575–88.
Piri, M., Sepehr, E., & Ghavidel, S.Z. (2023). Dosing of leonardite/struvite compounds as phosphorus fertilizers increased biomass and nutrient uptake in a calcareous soil. Journal of Cleaner Production, 430, 139723. 
Polatoglua, K., Karakoc, O.C., & Goren, N. (2013). Phytotoxic, DPPH scavenging, insecticidal activities and essential    oil composition of Achillea vermicularis, A. teretifolia and proposed chemotypes of A. biebersteinii (Asteraceae).    Industrial Crops and Products, 51(1), 35-45.
 Rahman, M. M., Salleh, M. M., Rashid, U., Ahsan, A., Hossain, M. M., & Ra, C. S. (2013). Production of slow release crystal fertilizer from wastewaters through struvite crystallization – A review. Arabian Journal of Chemistry, 7:139–155.
Rayment, G. E., & Higginson. F. R. (1992).  Australian laboratory handbook of soil and water chemical methods. Melbourne, in kata Press.
Ryu, H. D., & Lee, S. I. (2016). Struvite recovery from swine wastewater and its assessment as a fertilizer. Environmental Engineering Research, 21(1), 29–35. doi:10.4491/eer.2015.066.
Samadi, A., (2006). Phosphorus Sorption Characteristics in Relation to Soil Properties in Some Calcareous Soils of Western Azarbaijan Province. Journal of Agricultural Science and Technology, 8, 251-264.
Shi, Y., Yu, Y., Chang, E., Wang, R., Hong, Z., Cui, J., Zhang, F., Jiang, J., & Xu, R. (2023).  Effect  of  biochar incorporation  on  phosphorus  supplementation  and  availability  in  soil:  A  review. Journal of Soils and Sediments, 23 (2), 672–86.  doi:  10.1007/s11368-022-03359-w.
Shiba, C. N., & Freeman, N. (2016). Extraction and precipitation of phosphorus from sewage sludge. Waste Management, 60, 191–200.
Stevenson, F. J. (1982). Humus Chemistry: Genesis, Composition, Reactions. John Wiley & Sons, New York.
Szymanska, M., Comte, A., Tio, G., Vidal, C., Monnin, J., Smith, C. C., Nezelof, S., & Vulliez-Coady, L. (2019). The Besançon affective picture set-adult (BAPS-Adult): Development and validation. Psychiatry Research, 271, 31-38. doi: 10.1016/j.psychres.2018.11.005
Turkmen, N., Sari, F., & Velioglu, Y.S. (2005) The Effect of Cooking Methods on Total Phenolics and Antioxidant Activity of Selected Green Vegetables. Food Chemistry, 93, 713-718. http://dx.doi.org/10.1016/j.foodchem.2004.12.038
Valle, S. F., Giroto, A. S., Guimaraes, G. G. F., Nagel, K. A., Galinski, A., Cohnen, J., Jablonowski, N. D., & Ribeiro, C.  (2022). Co-fertilization  of  sulfur  and  struvite-phosphorus  in  a  slow-release  fertilizer  improves  soybean cultivation.  Frontiers in Plant Science, 13, 861574. doi:  10.3389/fpls.2022.861574.
Walkley, A. & Black, I. A. (1934). An examination of the Degtjare off method for determining soil organic matter, and a proposed modification of the chromic acidtitration method. Soil Science, 37 (1): 29-38.
Wang, C. Q., Luo, D., Zhang, X., Huang, R., Cao, Y. J., Liu, G. G., Zhang, Y. S., & Wang, H. (2022). Biochar-based slow-release of fertilizers for sustainable agriculture: A mini review.  Environmental Science and Ecotechnology, 10, 100167.  doi: 10.1016/j.ese.2022.100167.
Yoo, H. J., An, H. G., Park, S. Y., Ryu, O. H., Kim, H. Y., Seo, J. A., Hong, E. G., Shin, D. H., Kim, Y. H., Kim, S. G., Choi, K. M., Park, I. B., Yu, J. M., & Baik, S. H. (2008). Use of a real time continuous glucose monitoring system as a motivational device for poorly controlled type 2 diabetes. Diabetes Research and Clinical Practice, 82(1), 73-9. doi: 10.1016/j.diabres.2008.06.015
Zheng, J., Li. Y., Xu. D. P., Li. S., & Li. H. B. (2016). Spices for prevention and treatment of cancers. Nutrients, 8(8), 495.
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دوره 55، شماره 3
خرداد 1403
صفحه 401-413

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