تأثیر نانوذرات دی‎اکسیدتیتانیوم در کاهش اثر آرسنیک بر تنفس و شاخص‎های اکوفیزیولوژیک در خاکی با سطوح مختلف آرسنیک

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم خاک، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران.

2 هیت علمی مرکز تحقیفات آذربایجان شرقی

3 گروه علوم خاک، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران.

چکیده

به‎موجب توسعه روز افزون نانوفناوری، استفاده از آن در همه زمینه‎ها به‎ویژه در زمینه آلودگی‎های زیست‎محیطی به‎عنوان جاذب افزایش یافته است. بدین منظور آزمایشی به‎صورت فاکتوریل در قالب طرح کاملاً تصادفی با دو فاکتور، فاکتور آرسنیک در چهار سطح (صفر، 25، 50 و mg/kg 100) و فاکتور نانوذره TiO2 در سه سطح (صفر، 25/0 و 5/0 درصد وزنی نانوذره) و با سه تکرار در آزمایشگاه و در اتاقی تاریک با ˚C25 و در مدت 8 ماه و با استفاده از ظروف تنفس L 3/1 به اجرا درآمد. نتایج درصد تنفس تجمعی نشان داد که بیش‎ترین مطابقت تنفس تیمار شاهد با تیمار mg/kg 50 آرسنیک به‎همراه 5/0 درصد وزنی نانوذره به‎دست آمد. بیش‎ترین و کم‎ترین میزان تنفس به‎ترتیب در ماه‎های اول و هشتم انکوباسیون از تیمارهای شاهد و mg/kg 25 آرسنیک با اختلاف 78/33 درصد حاصل شد. با افزایش سطوح نانوذره میزان تنفس نیز افزایش یافت به‎طوری که بیش‎ترین میزان تنفس در ماه اول و از تیمار 5/0 درصد وزنی نانوذره به‎دست آمد. بیش‎ترین و کم‎ترین کربن زیست‎توده میکروبی و هم‎چنین کسر میکروبی به‎ترتیب از تیمارهای mg/kg 100 آرسنیک به‎همراه 25/0 درصد وزنی نانوذره و mg/kg 50 آرسنیک به‎همراه 5/0 درصد وزنی نانوذره حاصل شد و برعکس، بیش‎ترین و کم‎ترین کسر متابولیکی به‎ترتیب از تیمارهای mg/kg 50 آرسنیک به‎همراه 5/0 درصد وزنی نانوذره و mg/kg 100 آرسنیک به‎همراه 25/0 درصد وزنی نانوذره به‎دست آمد. تحلیل خوشه‎ای متغیرها نشان داد که متغیرهای کربن زیست‎توده میکروبی و کسر میکروبی در خوشه اول قرار گرفتند و در خوشه‎های دوم، سوم و چهارم نیز به‎ترتیب متغیرهای تنفس پایه، کسر متابولیکی و تنفس تجمعی قرار گرفتند. مطابق با نتایج این پژوهش، کاربرد 5/0 درصد وزنی نانوذره توانست اثرات سمی آرسنیک را کاهش داده و تنفس پایه، درصد تجمعی تنفس و تنفس ماهیانه را بهبود بخشد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Titanium Dioxide Nanoparticles on the Reduction of Arsenic Effect on Respiration and Soil Ecophysiological Indices in a Soil with Different Levels of Arsenic

نویسندگان [English]

  • Nader Khadem Moghadam Igdelou 1
  • Ahmad Golchin 1
  • Ahmad bybordi 2
  • Ali Beheshti Ale Agha 3
1 department of soil science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.
2 Faculty of East Azarbaijan Research Center
3 Department of Soil Science, Faculty of Agriculture, University of Razi, Kermanshah, Iran.
چکیده [English]

Due to the increasing development of nanotechnology, its use has increased in all fields, especially in the field of environmental pollution as an absorbent. For this purpose, a factorial experiment was conducted in a completely randomized design with two factors; arsenic factor at four levels (0, 25, 50 and, 100 mg/kg) and TiO2 factor at three levels (0, 0.25 and, 0.5% by weight) and three replications in the laboratory and in a dark room at 25˚C for 8 months using the 1.3 L respiratory jars. Cumulative respiration percentile results showed that the highest respiration control treatment matched with 50 mg/kg arsenic plus 0.5% TiO2 (w/w). The highest and the lowest respiration rates were obtained in the first and eighth months of incubation, respectively, with control and 25 mg/kg arsenic with a difference of 33.78%. As the nanoparticle levels increased, the respiration rate increased, so that the highest respiration rate was obtained in the first month of 0.5% TiO2 treatment. The highest and the lowest MBC, as well as qmic, was obtained in 100 mg/kg arsenic treatments plus 0.25% TiO2 (w/w) and 50 mg/kg arsenic plus 0.5% TiO2 (w/w), respectively. Conversely, the highest and the lowest qCO2 were obtained from 50 mg/kg arsenic plus 0.5% TiO2 (w/w) and 100 mg/kg arsenic with 0.5% TiO2 (w/w), respectively. Cluster analysis of the variables showed that the MBC and qmic variables were the first cluster and the second, third, and fourth clusters were the BR, qCO2, and cumulative respiration, respectively. According to the results of this study, the application of 0.5% TiO2 (w/w) can reduce the toxic effects of arsenic and improved BR, cumulative respiration rate, and monthly respiration.

کلیدواژه‌ها [English]

  • cumulative diagram
  • Euclidean distance
  • hierarchical cluster analysis
  • metabolic quotient
  • microbial quotient

مراجع

Anderson, T. H. and Domsch, K. H. (1990). Application of eco-physiological quotients (qCO2 and qD) on microbial biomasses from soils of different cropping histories. Soil Biology and Biochemistry, 22(2), 251-255.
Ansari, M. I. and Malik, A. (2007). Biosorption of nickel and cadmium by metal resistant bacterial isolates from agricultural soil irrigated with industrial wastewater. Bioresource Technology, 98(16), 3149-3153.
Aryabod, S., Fotovat, A., Khorasani, R. and Entezari, M. (2017). Cadmium adsorption on TiO2 Nanoparticles in soil suspensions. Iranian Journal of Soil and Water Research, 48(2), 349-358. (In Farsi)
Austin, A. T., Yahdjian, L., Stark, J. M., Belnap, J., Porporato, A., Norton, U., Ravetta, D. A. and Schaeffer, S. M. (2004). Water pulses and biogeochemical cycles in arid and semiarid ecosystems. Oecologia, 141(2), 221-235.
Bååth, E., Arnebrant, K. and Nordgren, A. (1991). Microbial biomass and ATP in smelter-polluted forest humus. Bulletin of Environmental Contamination and Toxicology, 47(2), 278-282.
Bremner, J. M. and Mulvaney, C. S. (1996). Kjeldhal Method. Method of Soil Analysis Part-2: Chemical & Microbiological Properties, American Society of Agronomy, Madison, 903-948.
Brookes, P. C. (1995). The use of microbial parameters in monitoring soil pollution by heavy metals. Biology and Fertility of Soils, 19(4), 269-279.
Brookes, P. C., Heijnen, C. E., McGrath, S. P. and Vance, E. D. (1986). Soil microbial biomass estimates in soils contaminated with metals. Soil Biology and Biochemistry, 18(4), 383-388.
Chander, K. and Brookes, P. C. (1991). Effects of heavy metals from past applications of sewage sludge on microbial biomass and organic matter accumulation in a sandy loam and silty loam UK soil. Soil Biology and Biochemistry, 23(10), 927-932.
Dai, J., Becquer, T., Rouiller, J. H., Reversat, G., Bernhard-Reversat, F. and Lavelle, P. (2004). Influence of heavy metals on C and N mineralisation and microbial biomass in Zn-, Pb-, Cu-, and Cd-contaminated soils. Applied Soil Ecology, 25(2), 99-109.
Dayani , L. and Raiesi, F. (2011). The role of compost in alleviating cadmium effects on microbial respiration and biomass, and phosphatase activity in soil. Journal of Water and Soil, 25(1), 161-173. (In Farsi)
Ding, W., Meng, L., Yin, Y., Cai, Z. and Zheng, X. (2007). CO2 emission in an intensively cultivated loam as affected by long-term application of organic manure and nitrogen fertilizer. Soil Biology and Biochemistry, 39(2), 669-679.
Duker, A. A., Carranza, E. and Hale, M. (2005). Arsenic geochemistry and health. Environment international, 31(5), 631-641.
Edvantoro, B. B., Naidu, R., Megharaj, M. and Singleton, I. (2003). Changes in microbial properties associated with long-term arsenic and DDT contaminated soils at disused cattle dip sites. Ecotoxicology and Environmental Safety, 55(3), 344-351.
Fang, C. and Moncrieff, J. B. (2005). The variation of soil microbial respiration with depth in relation to soil carbon composition. Plant and Soil, 268(1), 243-253.
Filip, Z. (2002). International approach to assessing soil quality by ecologically-related biological parameters. Agriculture, Ecosystems & Environment, 88(2), 169-174.
Frostegård, Å. and Bååth, E. (1996). The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil. Biology and Fertility of Soils, 22(1-2), 59-65.
Ge, Y., Schimel, J. P. and Holden, P. A. (2011). Evidence for negative effects of TiO2 and ZnO nanoparticles on soil bacterial communities. Environmental Science & Technology, 45(4), 1659-1664.
Gee, G. W. and Bauder, J. W. (1986). Physical and mineralogical methods. In: Klute, A. (Ed.), Methods of soil analysis, Part 1. Soil Science Society of America, Madison,WI, USA, pp. 383-411.
Ghosh, A. K., Bhattacharyya, P. and Pal, R. (2004). Effect of arsenic contamination on microbial biomass and its activities in arsenic contaminated soils of Gangetic West Bengal, India. Environment International, 30(4), 491-499.
Hemke, P. H. and Spark, D. L. (1996). Potassium. In: Method of soil analysis. Sparks, DL, Soil Science Society of America, Inc. American Society of Agronomy, Inc. Madison, Wisconsin, USA, 551-574.
Isermeyer, H. (1952). Eine einfache Methode zur Bestimmung der Bodenatmung und der Karbonate im Boden. Zeitschrift für Pflanzenernährung, Düngung, Bodenkunde, 56(1‐3), 26-38.
Jenkinson, D. S. (1981). Microbial biomass in soil: measurement and turnover. Soil Biochemistry, 5, 415-471.
Karimian-Shamsabadi, N., Ghorbani Dashtaki, Sh. and Raiesi, F. (2016). The effect of urban sewage sludge on chemical properties, soil basal respiration and microbial biomass carbon in a calcareous silty clay loam soil. Journal of Water and Soil Science, 21(1), 255-264. (In Farsi)
Khadem Moghadam Igdelou, N., Hatami, M., Rezaei, S., Bayat, M. and Lajayer, B. A. (2019). Induction of plant defense machinery against nanomaterials exposure. In M. Ghorbanpour and H. W. Shabir (Ed.), Advances in Phytonanotechnology. (pp. 241-263). Academic Press.
Khadem Moghdam Igdelou, N. and Golchin, A. (2018). The effect of titanium dioxide nanoparticles on soil heavy metals and plant yield. In: Proceedings of the first International Conference on Society and Environment, 11 Sept., Tehran University, Tehran, Iran. (In Farsi)
Khadem Moghdam Igdelou, N. and Golchin, A. (2019). Risk assessment of contamination of the country's soil and water resources with arsenic. Iranian Journal of Soil and Water Research, 50(7), 1595-1617. (In Farsi)
Landi, L., Renella, G., Moreno, J. L., Falchini, L. and Nannipieri, P. (2000). Influence of cadmium on the metabolic quotient, L-: D-glutamic acid respiration ratio and enzyme activity: microbial biomass ratio under laboratory conditions. Biology and Fertility of Soils, 32(1), 8-16.
Lata, S. and Samadder, S. R. (2016). Removal of arsenic from water using nano adsorbents and challenges: a review. Journal of Environmental Management, 166, 387-406.
Liao, X. Y., Chen, T. Bin, Xie, H. and Liu, Y. R. (2005). Soil As contamination and its risk assessment in areas near the industrial districts of Chenzhou City, Southern China. Environment International, 31(6), 791–798.
Lindsay, W. L. and Norvell, W. A. (1978). Development of a DTPA Soil Test for Zinc, Iron, Manganese, and Copper. Soil Science Society of America Journal, 42(3), 421-428.
Loeppert, R. H. and suarez, D. L. (1996). Carbonate and gypsum, in: 'Sparks, D. L., Page, A. L., Sumner, M.E., Tabatabai, M. A. and Helmke, P. A. (Ed.), Methods of Soil Analysis, Part3-Chemical Methods. Soil Science Society of America Inc., Madison, WI, USA. (pp. 437-474).
Mansouri, T. and Golchin, A. (2018). The effects of hematite nanoparticles on the concentrations of arsenic and some micronutrients of corn plant grown in contaminated soils. Journal of Water and Soil Conservation, 25(1), 1-34. (In Farsi)
Mansouri, T., Golchin, A. and Babaakbari Sari, M. (2016). The effect of arsenic on phosphorus, iron, zinc and manganese concentrations in soil and corn plant. Journal of Water and Soil, 31(2), 627-643. (In Farsi)
Marabottini, R., Stazi, S. R., Papp, R., Grego, S. and Moscatelli, M. C. (2013). Mobility and distribution of arsenic in contaminated mine soils and its effects on the microbial pool. Ecotoxicology and Environmental Safety, 96, 147-153.
Moscatelli, M. C., Lagomarsino, A., Marinari, S., De Angelis, P. and Grego, S. (2005). Soil microbial indices as bioindicators of environmental changes in a poplar plantation. Ecological Indicators, 5(3), 171-179.
Nelson, D. W. and Sommers, L. E. (1982). Total carbon, organic carbon, and organic matter. In: Sparks, D. L., Page, A. L., Helmke, P. A., Loeppert, R. H., Soltanpour, P. N., Tabatabai, M. A., Johnston, C. T. and Sumner, M. E. (Eds.), Methods of Soil Analysis, Part 2. Soil Science Society of America, Inc. Madison, Wisconsin, USA, pp. 539-579.
Olsen, S. R. (1954). Estimation of available phosphorus in soils by extraction with sodium bicarbonate. United States Department of Agriculture. Washington.
Prasad, P., George, J., Masto, R. E., Rout, T. K., Ram, L. C. and Selvi, V. A. (2013). Evaluation of microbial biomass and activity in different soils exposed to increasing level of arsenic pollution: a laboratory study. Soil and Sediment Contamination: An International Journal, 22(5), 483-497.
Rezaie, R. and Raiesi, F. (2016). Effect of superabsorbent polymers on soil microbial respiration and biomass under drought stress condition. Journal of Sol Biology, 3(2), 151-162. (In Farsi)
Shirzadeh, N., Ali-Asgharzad, N. and Najafi, N. (2013). Changes in microbial biomass carbon, ecophysiological indices, basal induced respiration of soil after incubation with -respiration and substrate levels different lead. Water and Soil Science, 23(2), 111-124. (In Farsi)
Shrestha, B., Acosta-Martinez, V., Cox, S. B., Green, M. J., Li, S. and Cañas-Carrell, J. E. (2013). An evaluation of the impact of multiwalled carbon nanotubes on soil microbial community structure and functioning. Journal of Hazardous Materials, 261, 188-197.
Sumner, M. E. and Miller, W. P. (1996). Cation exchange capacity and exchange coefficients. In: Sparks, D. L., Page, A. L., Helmke, P. A., Loeppert, R. H., Soltanpour, P. N., Tabatabai, M. A., Johnston, C. T., Sumner, M. E. (Eds.), Methods of Soil Analysis, Part 3. Soil Science Society of America, Inc., Madison, USA, pp. 1201-1229.
Wardle, D. A. and Ghani, A. (1995). A critique of the microbial metabolic quotient (qCO2) as a bioindicator of disturbance and ecosystem development. Soil Biology and Biochemistry, 27(12), 1601-1610.
Yang, Y., Campbell, C. D., Clark, L., Cameron, C. M. and Paterson, E. (2006). Microbial indicators of heavy metal contamination in urban and rural soils. Chemosphere, 63(11), 1942-1952.
Yazdan Panah, N., Fotovat, A., Lakzian, A. and Hagniya, Gh. H. (2008). The effect of heavy metals (Cd and Zn) on microbial respiration in calcareous and non-calcareous soils. Agricultural Sciences and Technology Journal, 22(1), 145-135. (In Farsi)
Zhang, J., Hao, Z., Zhang, Z., Yang, Y. and Xu, X. (2010). Kinetics of nitrate reductive denitrification by nanoscale zero-valent iron. Process Safety and Environmental Protection, 88(6), 439-445.
تحقیقات آب و خاک ایران
دوره 52، شماره 1
فروردین 1400
صفحه 1-14

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