ارزیابی کمی و کیفی توان تولید اکسین (IAA) برخی سویه‌های سیانوباکتر جداسازی شده از شالیزارهای استان گیلان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری بیولوژی و بیوتکنولوژی خاک دانشگاه تبریز

2 استاد بیولوژی خاک گروه علوم خاک دانشگاه تبریز

3 استاد دانشگاه علوم کشاورزی و منابع طبیعی ساری و پژوهشکدة ژنتیک و زیست فناوری طبرستان

4 دانشیار پژوهشکدة علوم پایة کاربردی جهاد دانشگاهی دانشگاه شهید بهشتی

چکیده

سیانوباکترها گروهی از پروکاریوت‌ها هستند که از نظر پتانسیل تولید مواد محرک رشد گیاه، به‌ویژه در زمینة تولید هورمون‏های گیاهی، کمتر دربارة آن‌ها تحقیق و مطالعه شده است. در این تحقیق توان تولید ایندول استیک اسید (IAA) سویه‏های سیانوباکتریایی جداسازی‌شده از شالیزارهای استان گیلان به طور کمّی و کیفی ارزیابی و پتانسیل آن‌ها بر شاخص‏های جوانه‏زنی بذرهای برنج بررسی شد. نتایج نشان داد توان تولید هورمون IAA در چند جدایة سیانوباکترهای شناسایی‌شده وجود دارد. در تیمار بدون اسید آمینة ال‌ـ تریپتوفان جدایه‏های GGuCy-34 و GGuCy-42 به ترتیب 98/14 و 83/10 میکروگرم IAA بر میلی‏لیتر بر کلروفیل a، در تیمار 100 میلی‏گرم بر میلی‏لیتر ال‌ـ تریپتوفان جدایه‌های GGuCy-34 و GGuCy-15 و GGuCy-42 به ترتیب 7/23 و 46/17 و 81/15 میکروگرم IAA بر میلی‏لیتر بر کلروفیل a، و در تیمار 500 میلی‏گرم بر میلی‏لیتر ال‌‌ـ تریپتوفان جدایه‏های GGuCy-15 و GGuCy-16 به ترتیب 16/29 و 61/21 میکروگرم IAA بر میلی‏لیتر بر کلروفیل a بیشترین مقادیر اکسین (IAA) را تولید کردند. نتایج نشان می‌دهد تولید میزان IAA به نوع جدایه و شرایط محیط کشت بستگی کامل دارد. انرژی و سرعت جوانه‏زنی بذرها تحت تأثیر سویه‏های GGuCy-25، GGuCy-42، GGuCy-41، GGuCy-26، و GGuCy-50 و وزن خشک ریشه‏چه و ساقه‏چه در بذر‌های تلقیح‌شده با سویه‏های GGuCy-42، GGuCy-50، و GGuCy-25 بیشترین افزایش را داشته است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Quantitative and Qualitative Evaluation of Auxin (IAA) Production Potential of Cyanobacteria, Isolated from Guilan Paddy Fields

نویسندگان [English]

  • Saheb Soodaee Mashaee 1
  • Naser Aliasgharzad 2
  • Ghorban Ali Nehmatzade 3
  • Neda Soltani 4
1 Ph.D. Candidate, of soil biology and biotechnology, Tabriz University
2 Professor, soil biology, department of soil sciences, Tabriz University
3 Professor, biotechnology, Agriculture and Natural Resources, University of Sari and Genetic and Biotechnology Institute of Tabarestan, Mazandaran
4 Associate professor, Department of Biology, Research Institute of Applied Sciences, Shahid Beheshti University, Tehran
چکیده [English]

Cyanobacteria represent a less investigated group of prokaryotes, in terms of their effect on plant growth, especially in relation with the production of phytohormones. The present research was aimed at evaluating Indole Acetic Acid (IAA) production potential of cyanobacteria strains isolated from Guilan paddy fields through the  two quantitative and qualitative methods, their potential being determined in terms of rice seed germination indices. The results obtained indicated that some cyanobacteria isolates could produce auxin hormone IAA.GGuCy-34 and GGuCy-42 isolates respectively produced 14.98 and 10.83 [µg IAA/(ml. Chl. a)] in no L-Trp treatment, GGuCy-34, GGuCy-15 and GGuCy-42 isolates respectively produced 23.7, 17.46 and 15.81 [µg IAA/(ml. Chl. a)] in 100 (mg L-Trp/ml) treatment and GGuCy-15 and GGuCy-16 isolates respectively produced 29.16 and 21.61 [µg IAA/(ml. Chl. a)] in 500 (mg L-Trp/ml) treatment. The results finally revealed that IAA production is highly correlated with the type of isolate and as well with the culture medium. Germination energy and germination rates increased in the cases of GGuCy-25, GGuCy-42, GGuCy-41, GGuCy-26 and GGuCy-50 isolates, and while dry radical weight as well as dry plumule weight increased in the cases of GGuCy-42, GGuCy-50, GGuCy-25 isolates. 

کلیدواژه‌ها [English]

  • IAA
  • rice
  • Tryptophan
  • germination
  • Cyanobacteria

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