Effect of application of salicylic acid and biofertilizers on physiological and biochemical characteristics of chickpea (Cicer arietinum) cultivars in rainfed conditions

Document Type : Research Paper

Authors

1 Ph.D. in Crop Physiology, Department of Agriculture and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran

2 Assistant Professor, Department of Agriculture and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran

Abstract

In order to investigate the effect of salicylic acid and biofertilizers on physiological and biochemical characteristics of rainfed chickpeas, a factorial split plot experiment was conducted in a randomized complete block design with three replications in the farm of Hamil Agricultural Jihad Center. Experimental treatments include: salicylic acid at three levels (non-use, half a millimeter and one millimolar) as the main factor of the experiment, biofertilizers at four levels (without the use of biofertilizer, Petabar 2, fertile phosphate 2 and the combined use of peta and fertile phosphate 2) And four chickpea cultivars (Adel, Mansour, Azkan and Goksu) were tested as factorial and as a sub-factor. The results of analysis of variance showed that all traits of chlorophyll a, chlorophyll b, total chlorophyll, proline, soluble protein, catalase, superoxide dismutase were affected by salicylic acid and biofertilizers at the level of 1%. Also, these traits except superoxide dismutase were Were also affected by the cultivar. The interaction of salicylic acid and biofertilizers had a significant effect on chlorophyll a, b, total, proline and catalase. The highest percentage of proline and soluble proteins was obtained in the simultaneous combination of salicylic acid and biofertilizers for Adel cultivar. With the use of 1 mM salicylic acid, the amount of proline increased by 21.6% compared to its non-use. Also, the highest amount of catalase enzyme was in the treatment of non-use of biofertilizers (0.2937 units per mg of protein) and the lowest amount was related to the combined use of fertile phosphate + 2 petabarur 2 (0.2290 units per mg of protein). . Therefore, it can be said that the simultaneous use of salicylic acid and biofertilizers increases the physiological activities of chickpea cultivars, also reduces the use of chemical fertilizers and is very environmentally beneficial.

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