University of Tehran Press Iranian Journal of Soil and Water Research 2008-479X 56 1 2025 03 21 Evaluating the role of plant growth-promoting bacteria in biological control of Salmonella typhimurium pathogen Evaluating the role of plant growth-promoting bacteria in biological control of Salmonella typhimurium pathogen 265 280 101499 10.22059/ijswr.2024.379505.669759 FA Motahare Abedinzadeh Department of Soil science, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran. 0009-0008-9983-4609 Naeimeh Enayatizamir Department of Soil Science, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran 0000-0001-5041-1471 Ehsan Shokri Department of Nanotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran 0000-0001-7417-5637 Shahla Kianamiri Department of Nanotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran 0000-0002-6521-1437 Journal Article 2024 07 20 Human pathogens such as Salmonella enter agricultural soils from various sources. Salmonella is one of the most common food-borne microorganisms and is a causative agent of infections shared between humans and animals. In the current study, some plant growth-promoting bacteria were used to control the pathogen Salmonella typhimurium. The results showed that, in order, 1) Bacillus rugosus CS5, 2) Bacillus vallismortis AS4, 3) Priestia aryabhattai CL1 and 4) Bacillus sp. SS4 had the highest inhibitory effect on the grrowth of Salmonella in liquid medium. However, in solid medium, the bacteria 1) B. vallismortis AS4, 2) B. rugosus CS5, 3) P. aryabhattai CL1 and 4) Bacillus sp. SS4 exhibited the greatest inhibition of this pathogen's growth. The ability to produce siderophores, proteases, lipases, and hydrogen cyanide was investigated in these four bacteria. All four bacteria were capable of producing siderophore. The highest protease production index, with values of 2.5 and 2.9, belonged to P. aryabhattai CL1 and B. rugosus CS5, respectively. B. vallismortis AS4 was capable of producing lipase. Both P. aryabhattai strain CL1 and B. rugosus strain CS5 had the ability to produce HCN, with values of 0.48 mg/mL and 0.2 mg/mL, respectively. The simple impact of four separate bacteria and a microbial consortium on the Salmonella population in soil was investigated. The results showed that the Salmonella population decreased 15 days after inoculation due to the treatments. The greatest reduction in the the population of this pathogen was related to the microbial consortium and the Bacillus sp. strain SS4 treatment, with a 92 % and 98 % reduction in population compared to the control, respectively. The findings indicate a positive effect of plant growth-promoting bacteria in soil for controlling S. typhimurium, which could reduce the negative impacts of the pathogen's presence in soil, thereby enhancing human health and food security. Human pathogens such as Salmonella enter agricultural soils from various sources. Salmonella is one of the most common food-borne microorganisms and is a causative agent of infections shared between humans and animals. In the current study, some plant growth-promoting bacteria were used to control the pathogen Salmonella typhimurium. The results showed that, in order, 1) Bacillus rugosus CS5, 2) Bacillus vallismortis AS4, 3) Priestia aryabhattai CL1 and 4) Bacillus sp. SS4 had the highest inhibitory effect on the grrowth of Salmonella in liquid medium. However, in solid medium, the bacteria 1) B. vallismortis AS4, 2) B. rugosus CS5, 3) P. aryabhattai CL1 and 4) Bacillus sp. SS4 exhibited the greatest inhibition of this pathogen's growth. The ability to produce siderophores, proteases, lipases, and hydrogen cyanide was investigated in these four bacteria. All four bacteria were capable of producing siderophore. The highest protease production index, with values of 2.5 and 2.9, belonged to P. aryabhattai CL1 and B. rugosus CS5, respectively. B. vallismortis AS4 was capable of producing lipase. Both P. aryabhattai strain CL1 and B. rugosus strain CS5 had the ability to produce HCN, with values of 0.48 mg/mL and 0.2 mg/mL, respectively. The simple impact of four separate bacteria and a microbial consortium on the Salmonella population in soil was investigated. The results showed that the Salmonella population decreased 15 days after inoculation due to the treatments. The greatest reduction in the the population of this pathogen was related to the microbial consortium and the Bacillus sp. strain SS4 treatment, with a 92 % and 98 % reduction in population compared to the control, respectively. The findings indicate a positive effect of plant growth-promoting bacteria in soil for controlling S. typhimurium, which could reduce the negative impacts of the pathogen's presence in soil, thereby enhancing human health and food security.

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